(Circular) (Six-base) MAPSORT of: pFN21AB8364.seq Check: 4288 from: 1 to: 6154 pFN21AB8364 6154 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3254 3254 Size: 6154 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3444 3964 278 Size: 53 83 186 936 1908 520 2468 Fragments arranged by size: 2468 1908 936 520 186 83 53 AccI GT'mk_AC Cuts at: 1937 3497 1937 Size: 1560 4594 Acc65I G'GTAC_C Cuts at: 3475 3475 Size: 6154 AclI AA'CG_TT Cuts at: 1563 4281 4654 1563 Size: 2718 373 3063 Fragments arranged by size: 3063 2718 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2047 2501 3343 4217 5601 5869 1632 Size: 415 454 842 874 1384 268 1917 Fragments arranged by size: 1917 1384 874 842 454 415 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5957 AflIII A'CryG_T Cuts at: 1203 2418 3407 5053 6030 1203 Size: 1215 989 1646 977 1327 Fragments arranged by size: 1646 1327 1215 989 977 AgeI A'CCGG_T Cuts at: 4985 4985 Size: 6154 AhdI GACnn_n'nnGTC Cuts at: 4883 4883 Size: 6154 AleI CACnn'nnGTG Cuts at: 1166 2732 1166 Size: 1566 4588 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6154 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2405 2437 2405 Size: 32 6122 AlwNI CAG_nnn'CTG Cuts at: 2218 2515 2878 3133 3897 5469 2218 Size: 297 363 255 764 1572 2903 Fragments arranged by size: 2903 1572 764 363 297 255 ApaI G_GGCC'C Cuts at: 2091 2091 Size: 6154 ApaLI G'TGCA_C Cuts at: 4211 5367 4211 Size: 1156 4998 ApoI r'AATT_y Cuts at: 1493 2579 3463 3689 3841 5950 1493 Size: 1086 884 226 152 2109 1697 Fragments arranged by size: 2109 1697 1086 884 226 152 AseI AT'TA_AT Cuts at: 160 1032 4708 160 Size: 872 3676 1606 Fragments arranged by size: 3676 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6154 AvaI C'yCGr_G Cuts at: 1870 1943 2055 2110 2121 3479 1870 Size: 73 112 55 11 1358 4545 Fragments arranged by size: 4545 1358 112 73 55 11 BamHI G'GATC_C Cuts at: 3484 3851 3484 Size: 367 5787 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3475 4930 618 Size: 325 217 627 1688 1455 1842 Fragments arranged by size: 1842 1688 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2091 2228 3278 3326 3350 3473 1391 Size: 700 137 1050 48 24 123 4072 Fragments arranged by size: 4072 1050 700 137 123 48 24 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2399 3251 928 Size: 971 500 852 3831 Fragments arranged by size: 3831 971 852 500 BbvCI CC'TCA_GC Cuts at: 2787 2832 3308 2787 Size: 45 476 5633 Fragments arranged by size: 5633 476 45 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2795 2829 4345 4379 1903 Size: 34 858 34 1516 34 3678 Fragments arranged by size: 3678 1516 858 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4046 5256 4046 Size: 1210 4944 BclI T'GATC_A Cuts at: 1548 1548 Size: 6154 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2157 2979 4765 Size: 107 122 71 631 605 485 822 1786 Cuts at: 4765 136 Size: 1525 Fragments arranged by size: 1786 1525 822 631 605 485 122 107 71 BglII A'GATC_T Cuts at: 2718 2922 6149 2718 Size: 204 3227 2723 Fragments arranged by size: 3227 2723 204 BlpI GC'TnA_GC Cuts at: 2222 3571 2222 Size: 1349 4805 Bme1580I G_kGCm'C Cuts at: 1790 2091 4215 5371 1790 Size: 301 2124 1156 2573 Fragments arranged by size: 2573 2124 1156 301 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6154 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3885 4843 442 Size: 416 53 83 399 24 2468 958 1753 Fragments arranged by size: 2468 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6154 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2258 2360 2381 2477 2507 2654 2939 3017 Size: 1019 102 21 96 30 147 285 78 Cuts at: 3017 3092 3209 3230 3310 3334 3361 4814 1239 Size: 75 117 21 80 24 27 1453 2579 Fragments arranged by size: 2579 1453 1019 285 147 117 102 96 80 78 75 30 27 24 21 21 Bpu10I CC'TnA_GC Cuts at: 2787 2832 3308 2787 Size: 45 476 5633 Fragments arranged by size: 5633 476 45 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4279 5144 5442 4279 Size: 865 298 4991 Fragments arranged by size: 4991 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2357 4817 882 Size: 617 858 2460 2219 Fragments arranged by size: 2460 2219 858 617 BsaAI yAC'GTr Cuts at: 493 1204 3410 3945 493 Size: 711 2206 535 2702 Fragments arranged by size: 2702 2206 711 535 BsaBI GATnn'nnATC Cuts at: 6148 6148 Size: 6154 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3441 3961 4343 4978 Size: 53 83 186 936 1908 520 382 635 Cuts at: 4978 275 Size: 1451 Fragments arranged by size: 1908 1451 936 635 520 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2937 4587 4985 5259 5406 5817 2937 Size: 1650 398 274 147 411 3274 Fragments arranged by size: 3274 1650 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2354 2384 2696 2726 2744 Size: 30 165 30 951 30 312 30 18 Cuts at: 2744 2774 1178 Size: 30 4558 Fragments arranged by size: 4558 951 312 165 30 30 30 30 30 18 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2098 2708 2879 3212 3364 1186 Size: 912 610 171 333 152 3976 Fragments arranged by size: 3976 912 610 333 171 152 BseYI C'CCAG_C Cuts at: 2882 5357 5853 2882 Size: 2475 496 3183 Fragments arranged by size: 3183 2475 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5581 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3604 4365 5393 1540 Size: 275 186 1603 761 1028 2301 Fragments arranged by size: 2301 1603 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3278 4215 4300 5371 3278 Size: 937 85 1071 4061 Fragments arranged by size: 4061 1071 937 85 BsmI GAATG_Cn' Cuts at: 3665 3758 3665 Size: 93 6061 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2091 2228 3278 3326 3350 3473 4215 Size: 399 301 137 1050 48 24 123 742 Cuts at: 4215 4300 5371 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 1050 742 399 301 137 123 85 48 24 BspEI T'CCGG_A Cuts at: 5817 5817 Size: 6154 BspHI T'CATG_A Cuts at: 4043 5773 4043 Size: 1730 4424 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2910 3254 3509 844 Size: 2066 344 255 3489 Fragments arranged by size: 3489 2066 344 255 BsrBI CCG'CTC Cuts at: 1394 1823 2073 2760 4041 1394 Size: 429 250 687 1281 3507 Fragments arranged by size: 3507 1281 687 429 250 BsrDI GCAATG_nn' Cuts at: 65 1248 2781 3006 4649 4823 65 Size: 1183 1533 225 1643 174 1396 Fragments arranged by size: 1643 1533 1396 1183 225 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2949 3204 4798 4985 1700 Size: 110 1139 255 1594 187 2869 Fragments arranged by size: 2869 1594 1139 255 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6154 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6154 BssSI C'ACGA_G Cuts at: 2080 4214 5226 2080 Size: 2134 1012 3008 Fragments arranged by size: 3008 2134 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6154 BstBI TT'CG_AA Cuts at: 4958 4958 Size: 6154 BstEII G'GTnAC_C Cuts at: 1184 2017 1184 Size: 833 5321 BstXI CCAn_nnnn'nTGG Cuts at: 2182 3385 2182 Size: 1203 4951 BstYI r'GATC_y Cuts at: 1972 2697 2718 2922 3484 3851 4236 4253 5705 Size: 725 21 204 562 367 385 17 1452 Cuts at: 5705 5791 5803 6149 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 725 562 385 367 346 204 86 21 17 12 Bsu36I CC'TnA_GG Cuts at: 2444 2675 2444 Size: 231 5923 BtgI C'CryG_G Cuts at: 513 1065 2745 3384 513 Size: 552 1680 639 3283 Fragments arranged by size: 3283 1680 639 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5415 Fragments arranged by size: 5415 683 56 BtsI GCAGTG_nn' Cuts at: 3670 4464 4484 3670 Size: 794 20 5340 Fragments arranged by size: 5340 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6154 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6119 DraI TTT'AAA Cuts at: 3458 3458 Size: 6154 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2026 5161 809 Size: 507 710 3135 1802 Fragments arranged by size: 3135 1802 710 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3036 3601 4490 Size: 54 1425 50 275 18 1206 565 889 Cuts at: 4490 6119 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1206 889 565 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3601 1537 Size: 275 1789 4090 Fragments arranged by size: 4090 1789 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2685 4084 1353 Size: 1332 1399 3423 Fragments arranged by size: 3423 1399 1332 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2507 4741 5115 5261 387 Size: 1064 138 918 2234 374 146 1280 Fragments arranged by size: 2234 1280 1064 918 374 146 138 EcoICRI GAG'CTC Cuts at: 3276 3276 Size: 6154 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2047 2258 2360 2381 2477 2501 2507 Size: 393 415 211 102 21 96 24 6 Cuts at: 2507 2654 2939 3017 3092 3209 3230 3310 3334 Size: 147 285 78 75 117 21 80 24 Cuts at: 3334 3343 3361 4217 4814 5601 5869 1239 Size: 9 18 856 597 787 268 1524 Fragments arranged by size: 1524 856 787 597 415 393 285 268 211 147 117 102 96 80 78 75 24 24 21 21 18 9 6 EcoNI CCTnn'n_nnAGG Cuts at: 3449 3449 Size: 6154 EcoO109I rG'GnC_Cy Cuts at: 2971 3289 2971 Size: 318 5836 EcoRI G'AATT_C Cuts at: 3463 3841 3463 Size: 378 5776 FspI TGC'GCA Cuts at: 2267 4660 2267 Size: 2393 3761 FspAI rTGC'GCAy Cuts at: 2267 2267 Size: 6154 HaeII r_GCGC'y Cuts at: 1678 5301 1678 Size: 3623 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2621 2653 2696 2728 4795 Size: 32 135 32 1049 32 43 32 2067 Cuts at: 4795 4827 4869 4901 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2067 1049 135 43 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2209 3498 3744 669 Size: 988 281 271 1289 246 3079 Fragments arranged by size: 3079 1289 988 281 271 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6154 HpaI GTT'AAC Cuts at: 3744 3744 Size: 6154 KpnI G_GTAC'C Cuts at: 3479 3479 Size: 6154 MfeI C'AATT_G Cuts at: 3753 3753 Size: 6154 MluI A'CGCG_T Cuts at: 6030 6030 Size: 6154 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5268 5452 1305 Size: 3963 184 2007 Fragments arranged by size: 3963 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3038 10 Size: 54 1425 343 1206 3126 Fragments arranged by size: 3126 1425 1206 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2015 2732 2895 4112 4471 4630 518 Size: 648 849 717 163 1217 359 159 2042 Fragments arranged by size: 2042 1217 849 717 648 359 163 159 MspA1I CmG'CkG Cuts at: 1606 2178 2455 3088 3115 3255 3570 4247 5395 Size: 572 277 633 27 140 315 677 1148 Cuts at: 5395 5640 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 633 572 315 277 245 140 27 NaeI GCC'GGC Cuts at: 2951 3206 2951 Size: 255 5899 NcoI C'CATG_G Cuts at: 513 1065 2745 513 Size: 552 1680 3922 Fragments arranged by size: 3922 1680 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6154 NgoMIV G'CCGG_C Cuts at: 2949 3204 2949 Size: 255 5899 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6154 NotI GC'GGCC_GC Cuts at: 3601 3601 Size: 6154 NspI r_CATG'y Cuts at: 1132 1229 2422 2509 2848 3512 5057 1132 Size: 97 1193 87 339 664 1545 2229 Fragments arranged by size: 2229 1545 1193 664 339 97 87 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6154 PciI A'CATG_T Cuts at: 2418 5053 2418 Size: 2635 3519 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6154 PfoI T'CCnGG_A Cuts at: 2235 2337 2700 2235 Size: 102 363 5689 Fragments arranged by size: 5689 363 102 PmeI GTTT'AAAC Cuts at: 3458 3458 Size: 6154 PmlI CAC'GTG Cuts at: 3410 3410 Size: 6154 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4223 4255 5736 5768 4223 Size: 32 1481 32 4609 Fragments arranged by size: 4609 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6154 PsiI TTA'TAA Cuts at: 3724 3724 Size: 6154 PspOMI G'GGCC_C Cuts at: 2087 2087 Size: 6154 PspXI vC'TCGA_Gb Cuts at: 2110 2110 Size: 6154 PstI C_TGCA'G Cuts at: 830 2385 2712 2874 3087 3506 830 Size: 1555 327 162 213 419 3478 Fragments arranged by size: 3478 1555 419 327 213 162 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6154 PvuII CAG'CTG Cuts at: 2455 3115 2455 Size: 660 5494 SacI G_AGCT'C Cuts at: 3278 3278 Size: 6154 SalI G'TCGA_C Cuts at: 1936 3496 1936 Size: 1560 4594 SbfI CC_TGCA'GG Cuts at: 3506 3506 Size: 6154 ScaI AGT'ACT Cuts at: 4402 6114 4402 Size: 1712 4442 SfcI C'TryA_G Cuts at: 826 1045 2381 2708 2870 3083 3175 3313 3502 Size: 219 1336 327 162 213 92 138 189 Cuts at: 3502 4637 5318 5509 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1336 1135 681 327 219 213 191 189 162 138 92 SfiI GGCCn_nnn'nGGCC Cuts at: 2979 2979 Size: 6154 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6154 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6154 SmaI CCC'GGG Cuts at: 1872 2057 3481 1872 Size: 185 1424 4545 Fragments arranged by size: 4545 1424 185 SmlI C'TyrA_G Cuts at: 820 1017 1943 2110 4258 5159 5421 820 Size: 197 926 167 2148 901 262 1553 Fragments arranged by size: 2148 1553 926 901 262 197 167 SnaBI TAC'GTA Cuts at: 493 3945 493 Size: 3452 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6154 SphI G_CATG'C Cuts at: 1132 3512 1132 Size: 2380 3774 SspI AAT'ATT Cuts at: 5 52 4078 5 Size: 47 4026 2081 Fragments arranged by size: 4026 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6154 StyI C'CwwG_G Cuts at: 513 1065 2745 3450 3593 513 Size: 552 1680 705 143 3074 Fragments arranged by size: 3074 1680 705 552 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2285 2672 4181 4198 4351 4536 4981 Size: 1255 705 387 1509 17 153 185 445 Cuts at: 4981 325 Size: 1498 Fragments arranged by size: 1509 1498 1255 705 445 387 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4400 6112 96 Size: 274 80 33 51 1443 2423 1712 138 Fragments arranged by size: 2423 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2354 2386 6114 6146 2354 Size: 32 3728 32 2362 Fragments arranged by size: 3728 2362 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6154 XbaI T'CTAG_A Cuts at: 3490 3490 Size: 6154 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3100 3100 Size: 6154 XhoI C'TCGA_G Cuts at: 1943 2110 1943 Size: 167 5987 XmaI C'CCGG_G Cuts at: 1870 2055 3479 1870 Size: 185 1424 4545 Fragments arranged by size: 4545 1424 185 XmnI GAAnn'nnTTC Cuts at: 4283 4283 Size: 6154 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3442 3962 276 Size: 53 83 186 936 1908 520 2468 Fragments arranged by size: 2468 1908 936 520 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI KasI NarI NruI NsiI PacI PpuMI PsrI RsrII SacII SanDI SapI SexAI SfoI SrfI SwaI