Restriction map for
pFN21AB9792
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaII AviII AvrII
BalI BamHI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BsrI Bst1107I BstBI BstEII BstF5I
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DdeI DraI
DraII DrdI EaeI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FokI FseI FspI HincII
HindII HindIII HinfI HpaI HphI
KpnI Ksp632I KspI MaeI MaeIII
MfeI MluNI MroI MscI MseI
MslI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmeI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfaNI SfcI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp45I
Tsp509I Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AflIII 275 ACGCGT A^C[GA][CT]GT
AvaI 309 CTCGGG C^[CT]CG[GA]G
BanI 304 GGCGCC G^G[CT][GA]CC
BanII 517 GGGCTC G[GA]GC[CT]^C
BbeI 304 GGCGCC GGCGC^C
BbrPI 118 CACGTG CAC^GTG
BbsI 576 GAAGAC GAAGAC(2/6)
BcnI 561 CCGGG CC^[GC]GG
BpmI 595 CTGGAG CTGGAG(16/14)
BpuAI 576 GAAGAC GAAGAC(2/6)
BsaBI 57 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 444 GGTCTC GGTCTC(1/5)
BsmAI 445 GTCTC GTCTC(1/5)
BsoBI 309 CTCGGG C^[CT]CG[GA]G
BspMI 105 GCAGGT ACCTGC(4/8)
BssSI 507 CTCGTG CACGAG(-5/-1)
BstAPI 6 GCACTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 56 AGATCC [GA]^GATC[CT]
Cfr13I 191 GGCCC G^G[ACGT]CC
DraIII 7 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 581 CCATGG C^C[GA][CT]GG
FauI 160 GCGGG CCCGC(4/6)
HapII 561 CCGG C^CGG
HpaII 561 CCGG C^CGG
KasI 304 GGCGCC G^GCGCC
MamI 57 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 56 AGATCC [GA]^GATC[CT]
MluI 275 ACGCGT A^CGCGT
MspI 561 CCGG C^CGG
NarI 304 GGCGCC GG^CGCC
NciI 561 CCGGG CC^[GC]GG
NcoI 581 CCATGG C^CATGG
NlaIII 582 CATG CATG^
PmaCI 118 CACGTG CAC^GTG
PmlI 118 CACGTG CAC^GTG
Sau96I 191 GGCCC G^G[ACGT]CC
SexAI 440 ACCAGGT A^CC[AT]GGT
SfoI 304 GGCGCC GGC^GCC
TspRI 7 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 441 CCAGGTCTCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 56 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 304 GGCGCC G[GA]^CG[CT]C
384 GGCGTC
AfaI 426 GTAC GT^AC
546 GTAC
AlwI 27 GATCC GGATC(4/5)
57 GATCC
AlwNI 37 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
142 CAGGAGCTG
Aor51HI 236 AGCGCT AGC^GCT
356 AGCGCT
BsaAI 118 CACGTG [CT]AC^GT[GA]
423 CACGTA
BsaHI 304 GGCGCC G[GA]^CG[CT]C
384 GGCGTC
BsgI 4 CTGCAC GTGCAG(16/14)
13 GTGCAG
BsmFI 387 GTCCC GGGAC(10/14)
551 GTCCC
BssHII 18 GCGCGC G^CGCGC
20 GCGCGC
Eco47III 236 AGCGCT AGC^GCT
356 AGCGCT
HgaI 277 GCGTC GACGC(5/10)
385 GCGTC
Hin1I 304 GGCGCC G[GA]^CG[CT]C
384 GGCGTC
MaeII 119 ACGT A^CGT
424 ACGT
MspA1I 158 CAGCGG C[AC]G^C[GT]G
168 CAGCGG
NlaIV 304 GGCGCC GG[ACGT]^[ACGT]CC
497 GGTTCC
RsaI 426 GTAC GT^AC
546 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AccII AciI AluI BbvI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstNI BstUI Cac8I CfoI DpnII
EcoRII EcoT14I Fnu4HI HaeII HaeIII
HhaI HinP1I ItaI MboI MboII
MnlI MvaI MvnI MwoI NdeII
PflMI Sau3AI ScrFI StyI TaqI
TseI TthHB8I Van91I