(Circular) (Six-base) MAPSORT of: pFN21AB9783.seq Check: 2510 from: 1 to: 5458 pFN21AB9783 5458 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2020 2650 2020 Size: 630 4828 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2550 3268 278 Size: 53 83 186 936 1014 718 2468 Fragments arranged by size: 2468 1014 936 718 186 83 53 AccI GT'mk_AC Cuts at: 1937 2801 1937 Size: 864 4594 Acc65I G'GTAC_C Cuts at: 2779 2779 Size: 5458 AclI AA'CG_TT Cuts at: 1563 3585 3958 1563 Size: 2022 373 3063 Fragments arranged by size: 3063 2022 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2613 3521 4905 5173 1632 Size: 981 908 1384 268 1917 Fragments arranged by size: 1917 1384 981 908 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5261 AflIII A'CryG_T Cuts at: 1203 4357 5334 1203 Size: 3154 977 1327 Fragments arranged by size: 3154 1327 977 AgeI A'CCGG_T Cuts at: 4289 4289 Size: 5458 AhdI GACnn_n'nnGTC Cuts at: 4187 4187 Size: 5458 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5458 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2285 1184 Size: 1101 4357 AlwNI CAG_nnn'CTG Cuts at: 2254 3201 4773 2254 Size: 947 1572 2939 Fragments arranged by size: 2939 1572 947 ApaI G_GGCC'C Cuts at: 2393 2487 2628 2393 Size: 94 141 5223 Fragments arranged by size: 5223 141 94 ApaLI G'TGCA_C Cuts at: 3515 4671 3515 Size: 1156 4302 ApoI r'AATT_y Cuts at: 1493 2767 2993 3145 5254 1493 Size: 1274 226 152 2109 1697 Fragments arranged by size: 2109 1697 1274 226 152 AseI AT'TA_AT Cuts at: 160 1032 4012 160 Size: 872 2980 1606 Fragments arranged by size: 2980 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5458 AvaI C'yCGr_G Cuts at: 1870 1943 2783 1870 Size: 73 840 4545 Fragments arranged by size: 4545 840 73 BamHI G'GATC_C Cuts at: 2788 3155 2788 Size: 367 5091 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2368 2398 2779 4234 618 Size: 325 217 627 581 30 381 1455 1842 Fragments arranged by size: 1842 1455 627 581 381 325 217 30 BanII G_rGCy'C Cuts at: 1391 2279 2345 2393 2487 2520 2628 2777 1391 Size: 888 66 48 94 33 108 149 4072 Fragments arranged by size: 4072 888 149 108 94 66 48 33 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4487 BbvCI CC'TCA_GC Cuts at: 2724 2724 Size: 5458 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3649 3683 1903 Size: 34 1712 34 3678 Fragments arranged by size: 3678 1712 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3350 4560 3350 Size: 1210 4248 BclI T'GATC_A Cuts at: 1548 1548 Size: 5458 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2331 2482 2615 Size: 107 122 71 631 605 659 151 133 Cuts at: 2615 4069 136 Size: 1454 1525 Fragments arranged by size: 1525 1454 659 631 605 151 133 122 107 71 BglII A'GATC_T Cuts at: 5453 5453 Size: 5458 BlpI GC'TnA_GC Cuts at: 2875 2875 Size: 5458 Bme1580I G_kGCm'C Cuts at: 1790 2327 2371 2393 2403 2487 2628 3519 4675 Size: 537 44 22 10 84 141 891 1156 Cuts at: 4675 1790 Size: 2573 Fragments arranged by size: 2573 1156 891 537 141 84 44 22 10 BmgBI CAC'GTC Cuts at: 1318 2322 1318 Size: 1004 4454 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3189 4147 442 Size: 416 53 83 399 24 1772 958 1753 Fragments arranged by size: 1772 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5458 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2513 4118 1239 Size: 1274 1605 2579 Fragments arranged by size: 2579 1605 1274 Bpu10I CC'TnA_GC Cuts at: 2373 2724 2373 Size: 351 5107 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2638 3583 4448 4746 2638 Size: 945 865 298 3350 Fragments arranged by size: 3350 945 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2187 2733 4121 882 Size: 617 688 546 1388 2219 Fragments arranged by size: 2219 1388 688 617 546 BsaAI yAC'GTr Cuts at: 493 1204 2127 3249 493 Size: 711 923 1122 2702 Fragments arranged by size: 2702 1122 923 711 BsaBI GATnn'nnATC Cuts at: 5452 5452 Size: 5458 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2547 3265 3647 4282 Size: 53 83 186 936 1014 718 382 635 Cuts at: 4282 275 Size: 1451 Fragments arranged by size: 1451 1014 936 718 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2052 3891 4289 4563 4710 5121 2052 Size: 1839 398 274 147 411 2389 Fragments arranged by size: 2389 1839 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2631 2661 1178 Size: 30 165 30 1228 30 3975 Fragments arranged by size: 3975 1228 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2192 2201 2612 1186 Size: 1006 9 411 4032 Fragments arranged by size: 4032 1006 411 9 BseYI C'CCAG_C Cuts at: 4661 5157 4661 Size: 496 4962 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4885 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2908 3669 4697 1540 Size: 275 186 907 761 1028 2301 Fragments arranged by size: 2301 1028 907 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2145 2279 2520 3519 3604 4675 2145 Size: 134 241 999 85 1071 2928 Fragments arranged by size: 2928 1071 999 241 134 85 BsmI GAATG_Cn' Cuts at: 2969 3062 2969 Size: 93 5365 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2145 2279 2327 2345 2371 2393 2403 Size: 399 355 134 48 18 26 22 10 Cuts at: 2403 2487 2520 2628 2777 3519 3604 4675 1391 Size: 84 33 108 149 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 355 149 134 108 85 84 48 33 26 22 18 10 BspEI T'CCGG_A Cuts at: 2052 5121 2052 Size: 3069 2389 BspHI T'CATG_A Cuts at: 3347 5077 3347 Size: 1730 3728 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2020 2277 2457 2650 2813 844 Size: 1176 257 180 193 163 3489 Fragments arranged by size: 3489 1176 257 193 180 163 BsrBI CCG'CTC Cuts at: 1394 1823 2419 3345 1394 Size: 429 596 926 3507 Fragments arranged by size: 3507 926 596 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3953 4127 65 Size: 1183 2705 174 1396 Fragments arranged by size: 2705 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2536 4102 4289 1700 Size: 110 726 1566 187 2869 Fragments arranged by size: 2869 1566 726 187 110 BsrGI T'GTAC_A Cuts at: 96 2256 96 Size: 2160 3298 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5458 BssSI C'ACGA_G Cuts at: 3518 4530 3518 Size: 1012 4446 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5458 BstBI TT'CG_AA Cuts at: 4262 4262 Size: 5458 BstEII G'GTnAC_C Cuts at: 1184 2662 1184 Size: 1478 3980 BstYI r'GATC_y Cuts at: 1972 2788 3155 3540 3557 5009 5095 5107 5453 Size: 816 367 385 17 1452 86 12 346 Cuts at: 5453 1972 Size: 1977 Fragments arranged by size: 1977 1452 816 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2346 2346 Size: 5458 BtgI C'CryG_G Cuts at: 513 1065 2004 2040 2479 513 Size: 552 939 36 439 3492 Fragments arranged by size: 3492 939 552 439 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4719 Fragments arranged by size: 4719 683 56 BtsI GCAGTG_nn' Cuts at: 2752 2974 3768 3788 2752 Size: 222 794 20 4422 Fragments arranged by size: 4422 794 222 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5458 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5423 DraI TTT'AAA Cuts at: 2762 2762 Size: 5458 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4465 809 Size: 507 3149 1802 Fragments arranged by size: 3149 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2616 2905 3794 Size: 54 1425 50 275 18 786 289 889 Cuts at: 3794 5423 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 786 289 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2905 1537 Size: 275 1093 4090 Fragments arranged by size: 4090 1093 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2133 2210 2525 2589 3388 1353 Size: 780 77 315 64 799 3423 Fragments arranged by size: 3423 799 780 315 77 64 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4045 4419 4565 387 Size: 1064 138 2456 374 146 1280 Fragments arranged by size: 2456 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2277 2518 2277 Size: 241 5217 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2513 2613 3521 4118 4905 5173 1239 Size: 393 881 100 908 597 787 268 1524 Fragments arranged by size: 1524 908 881 787 597 393 268 100 EcoNI CCTnn'n_nnAGG Cuts at: 2618 2618 Size: 5458 EcoO109I rG'GnC_Cy Cuts at: 2389 2390 2624 2625 2389 Size: 1 234 1 5222 Fragments arranged by size: 5222 234 1 1 EcoRI G'AATT_C Cuts at: 2767 3145 2767 Size: 378 5080 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2326 2410 2326 Size: 84 5374 FspI TGC'GCA Cuts at: 2583 3964 2583 Size: 1381 4077 HaeII r_GCGC'y Cuts at: 1678 4605 1678 Size: 2927 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2186 2218 2502 2534 4099 Size: 32 135 32 614 32 284 32 1565 Cuts at: 4099 4131 4173 4205 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1565 614 284 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2802 3048 669 Size: 988 281 864 246 3079 Fragments arranged by size: 3079 988 864 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5458 HpaI GTT'AAC Cuts at: 3048 3048 Size: 5458 KpnI G_GTAC'C Cuts at: 2783 2783 Size: 5458 MfeI C'AATT_G Cuts at: 3057 3057 Size: 5458 MluI A'CGCG_T Cuts at: 5334 5334 Size: 5458 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4572 4756 1305 Size: 3267 184 2007 Fragments arranged by size: 3267 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3636 Fragments arranged by size: 3636 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3416 3775 3934 518 Size: 648 2250 359 159 2042 Fragments arranged by size: 2250 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2251 2337 2481 2723 2874 3551 4699 4944 Size: 645 86 144 242 151 677 1148 245 Cuts at: 4944 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 645 245 242 151 144 86 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3967 Fragments arranged by size: 3967 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5458 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5458 NotI GC'GGCC_GC Cuts at: 2905 2905 Size: 5458 NspI r_CATG'y Cuts at: 1132 1229 2816 4361 1132 Size: 97 1587 1545 2229 Fragments arranged by size: 2229 1587 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5458 PciI A'CATG_T Cuts at: 4357 4357 Size: 5458 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5458 PmeI GTTT'AAAC Cuts at: 2762 2762 Size: 5458 PmlI CAC'GTG Cuts at: 2127 2127 Size: 5458 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2018 2050 3527 3559 5040 5072 2018 Size: 32 1477 32 1481 32 2404 Fragments arranged by size: 2404 1481 1477 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5458 PsiI TTA'TAA Cuts at: 3028 3028 Size: 5458 PspOMI G'GGCC_C Cuts at: 2389 2483 2624 2389 Size: 94 141 5223 Fragments arranged by size: 5223 141 94 PstI C_TGCA'G Cuts at: 830 2250 2529 2556 2810 830 Size: 1420 279 27 254 3478 Fragments arranged by size: 3478 1420 279 254 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5458 PvuII CAG'CTG Cuts at: 2723 2723 Size: 5458 SacI G_AGCT'C Cuts at: 2279 2520 2279 Size: 241 5217 SacII CC_GC'GG Cuts at: 2482 2482 Size: 5458 SalI G'TCGA_C Cuts at: 1936 2800 1936 Size: 864 4594 SapI GCTCTTCn'nnn_ Cuts at: 2133 2525 2133 Size: 392 5066 SbfI CC_TGCA'GG Cuts at: 2529 2556 2810 2529 Size: 27 254 5177 Fragments arranged by size: 5177 254 27 ScaI AGT'ACT Cuts at: 3706 5418 3706 Size: 1712 3746 SfcI C'TryA_G Cuts at: 826 1045 2246 2525 2552 2806 3941 4622 4813 Size: 219 1201 279 27 254 1135 681 191 Cuts at: 4813 826 Size: 1471 Fragments arranged by size: 1471 1201 1135 681 279 254 219 191 27 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5458 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5458 SmaI CCC'GGG Cuts at: 1872 2785 1872 Size: 913 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2653 3562 4463 4725 820 Size: 197 926 710 909 901 262 1553 Fragments arranged by size: 1553 926 909 901 710 262 197 SnaBI TAC'GTA Cuts at: 493 3249 493 Size: 2756 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5458 SphI G_CATG'C Cuts at: 1132 2816 1132 Size: 1684 3774 SspI AAT'ATT Cuts at: 5 52 3382 5 Size: 47 3330 2081 Fragments arranged by size: 3330 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5458 StyI C'CwwG_G Cuts at: 513 1065 2004 2289 2394 2897 513 Size: 552 939 285 105 503 3074 Fragments arranged by size: 3074 939 552 503 285 105 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2211 2687 3485 3502 3655 3840 4285 Size: 1255 631 476 798 17 153 185 445 Cuts at: 4285 325 Size: 1498 Fragments arranged by size: 1498 1255 798 631 476 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2118 2256 3704 Size: 274 80 33 51 1443 141 138 1448 Cuts at: 3704 5416 96 Size: 1712 138 Fragments arranged by size: 1712 1448 1443 274 141 138 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2631 2663 5418 5450 2631 Size: 32 2755 32 2639 Fragments arranged by size: 2755 2639 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5458 XbaI T'CTAG_A Cuts at: 2794 2794 Size: 5458 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5458 XmaI C'CCGG_G Cuts at: 1870 2783 1870 Size: 913 4545 XmnI GAAnn'nnTTC Cuts at: 3587 3587 Size: 5458 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2548 3266 276 Size: 53 83 186 936 1014 718 2468 Fragments arranged by size: 2468 1014 936 718 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI XcmI