(Circular) (Six-base) MAPSORT of: pFN21AB5487.seq Check: 520 from: 1 to: 5449 pFN21AB5487 5449 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3259 278 Size: 53 83 186 936 1723 2468 Fragments arranged by size: 2468 1723 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2574 2792 1937 Size: 637 218 4594 Fragments arranged by size: 4594 637 218 Acc65I G'GTAC_C Cuts at: 2770 2770 Size: 5449 AclI AA'CG_TT Cuts at: 1563 3576 3949 1563 Size: 2013 373 3063 Fragments arranged by size: 3063 2013 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2237 2714 3512 4896 5164 1632 Size: 605 477 798 1384 268 1917 Fragments arranged by size: 1917 1384 798 605 477 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5252 AflIII A'CryG_T Cuts at: 1203 2022 4348 5325 1203 Size: 819 2326 977 1327 Fragments arranged by size: 2326 1327 977 819 AgeI A'CCGG_T Cuts at: 4280 4280 Size: 5449 AhdI GACnn_n'nnGTC Cuts at: 4178 4178 Size: 5449 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5449 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2453 1184 Size: 1269 4180 AlwNI CAG_nnn'CTG Cuts at: 2342 2692 3192 4764 2342 Size: 350 500 1572 3027 Fragments arranged by size: 3027 1572 500 350 ApaLI G'TGCA_C Cuts at: 2026 3506 4662 2026 Size: 1480 1156 2813 Fragments arranged by size: 2813 1480 1156 ApoI r'AATT_y Cuts at: 1493 2758 2984 3136 5245 1493 Size: 1265 226 152 2109 1697 Fragments arranged by size: 2109 1697 1265 226 152 AseI AT'TA_AT Cuts at: 160 1032 4003 160 Size: 872 2971 1606 Fragments arranged by size: 2971 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5449 AvaI C'yCGr_G Cuts at: 1870 1943 2636 2774 1870 Size: 73 693 138 4545 Fragments arranged by size: 4545 693 138 73 BamHI G'GATC_C Cuts at: 2779 3146 2779 Size: 367 5082 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2084 2123 2429 2770 4225 Size: 325 217 627 297 39 306 341 1455 Cuts at: 4225 618 Size: 1842 Fragments arranged by size: 1842 1455 627 341 325 306 297 217 39 BanII G_rGCy'C Cuts at: 1391 2768 1391 Size: 1377 4072 BbeI G_GCGC'C Cuts at: 2088 2127 2088 Size: 39 5410 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4478 BbvCI CC'TCA_GC Cuts at: 2496 2496 Size: 5449 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3640 3674 1903 Size: 34 50 34 1619 34 3678 Fragments arranged by size: 3678 1619 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3341 4551 3341 Size: 1210 4239 BclI T'GATC_A Cuts at: 1548 1548 Size: 5449 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4060 136 Size: 107 122 71 631 605 2388 1525 Fragments arranged by size: 2388 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5444 5444 Size: 5449 BlpI GC'TnA_GC Cuts at: 2866 2866 Size: 5449 Bme1580I G_kGCm'C Cuts at: 1790 2030 2432 3510 4666 1790 Size: 240 402 1078 1156 2573 Fragments arranged by size: 2573 1156 1078 402 240 BmgBI CAC'GTC Cuts at: 1318 2590 1318 Size: 1272 4177 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3180 4138 442 Size: 416 53 83 399 24 1763 958 1753 Fragments arranged by size: 1763 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2516 1056 Size: 1460 3989 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2253 4109 1239 Size: 1014 1856 2579 Fragments arranged by size: 2579 1856 1014 Bpu10I CC'TnA_GC Cuts at: 2297 2496 2297 Size: 199 5250 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3574 4439 4737 3574 Size: 865 298 4286 Fragments arranged by size: 4286 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4112 882 Size: 617 2613 2219 Fragments arranged by size: 2613 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2410 3240 493 Size: 711 1206 830 2702 Fragments arranged by size: 2702 1206 830 711 BsaBI GATnn'nnATC Cuts at: 5443 5443 Size: 5449 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2085 2124 3256 3638 Size: 53 83 186 936 552 39 1132 382 Cuts at: 3638 4273 275 Size: 635 1451 Fragments arranged by size: 1451 1132 936 635 552 382 186 83 53 39 BsaWI w'CCGG_w Cuts at: 2157 3882 4280 4554 4701 5112 2157 Size: 1725 398 274 147 411 2494 Fragments arranged by size: 2494 1725 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5224 Fragments arranged by size: 5224 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2713 1186 Size: 1527 3922 BseYI C'CCAG_C Cuts at: 2331 4652 5148 2331 Size: 2321 496 2632 Fragments arranged by size: 2632 2321 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2432 1163 Size: 573 696 4180 Fragments arranged by size: 4180 696 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2095 2899 3660 4688 1540 Size: 275 186 94 804 761 1028 2301 Fragments arranged by size: 2301 1028 804 761 275 186 94 BsiHKAI G_wGCw'C Cuts at: 2030 3510 3595 4666 2030 Size: 1480 85 1071 2813 Fragments arranged by size: 2813 1480 1071 85 BsmI GAATG_Cn' Cuts at: 2325 2960 3053 2325 Size: 635 93 4721 Fragments arranged by size: 4721 635 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2432 2768 3510 3595 4666 1391 Size: 399 240 402 336 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 402 399 336 240 85 BspEI T'CCGG_A Cuts at: 5112 5112 Size: 5449 BspHI T'CATG_A Cuts at: 3338 5068 3338 Size: 1730 3719 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2192 2804 844 Size: 1348 612 3489 Fragments arranged by size: 3489 1348 612 BsrBI CCG'CTC Cuts at: 1394 1823 3336 1394 Size: 429 1513 3507 Fragments arranged by size: 3507 1513 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3944 4118 65 Size: 1183 2696 174 1396 Fragments arranged by size: 2696 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2090 4093 4280 1700 Size: 110 280 2003 187 2869 Fragments arranged by size: 2869 2003 280 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5449 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5449 BssSI C'ACGA_G Cuts at: 3509 4521 3509 Size: 1012 4437 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5449 BstBI TT'CG_AA Cuts at: 4253 4253 Size: 5449 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5449 BstXI CCAn_nnnn'nTGG Cuts at: 2134 2134 Size: 5449 BstYI r'GATC_y Cuts at: 1972 2779 3146 3531 3548 5000 5086 5098 5444 Size: 807 367 385 17 1452 86 12 346 Cuts at: 5444 1972 Size: 1977 Fragments arranged by size: 1977 1452 807 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2061 2115 513 Size: 552 996 54 3847 Fragments arranged by size: 3847 996 552 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2017 487 Size: 683 56 791 3919 Fragments arranged by size: 3919 791 683 56 BtsI GCAGTG_nn' Cuts at: 2311 2965 3759 3779 2311 Size: 654 794 20 3981 Fragments arranged by size: 3981 794 654 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5449 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5414 DraI TTT'AAA Cuts at: 2753 2753 Size: 5449 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4456 809 Size: 507 3140 1802 Fragments arranged by size: 3140 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2092 2454 2896 Size: 54 1425 50 275 18 262 362 442 Cuts at: 2896 3785 5414 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 442 362 275 262 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2092 2896 1537 Size: 275 280 804 4090 Fragments arranged by size: 4090 804 280 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2256 2690 3379 1353 Size: 903 434 689 3423 Fragments arranged by size: 3423 903 689 434 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4036 4410 4556 387 Size: 1064 138 2447 374 146 1280 Fragments arranged by size: 2447 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2237 2253 2714 3512 4109 4896 5164 Size: 393 605 16 461 798 597 787 268 Cuts at: 5164 1239 Size: 1524 Fragments arranged by size: 1524 798 787 605 597 461 393 268 16 EcoO109I rG'GnC_Cy Cuts at: 2689 2689 Size: 5449 EcoRI G'AATT_C Cuts at: 2758 3136 2758 Size: 378 5071 FspI TGC'GCA Cuts at: 3955 3955 Size: 5449 HaeII r_GCGC'y Cuts at: 1678 2088 2127 2583 4596 1678 Size: 410 39 456 2013 2531 Fragments arranged by size: 2531 2013 456 410 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4090 4122 4164 4196 1373 Size: 32 135 32 2518 32 42 32 2626 Fragments arranged by size: 2626 2518 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2491 2793 3039 669 Size: 988 281 553 302 246 3079 Fragments arranged by size: 3079 988 553 302 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5449 HpaI GTT'AAC Cuts at: 3039 3039 Size: 5449 KasI G'GCGC_C Cuts at: 2084 2123 2084 Size: 39 5410 KpnI G_GTAC'C Cuts at: 2774 2774 Size: 5449 MfeI C'AATT_G Cuts at: 3048 3048 Size: 5449 MluI A'CGCG_T Cuts at: 5325 5325 Size: 5449 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2094 4563 4747 1305 Size: 789 2469 184 2007 Fragments arranged by size: 2469 2007 789 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2456 10 Size: 54 1425 343 624 3003 Fragments arranged by size: 3003 1425 624 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3407 3766 3925 518 Size: 648 2241 359 159 2042 Fragments arranged by size: 2241 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2063 2130 2314 2865 3542 4690 4935 1606 Size: 457 67 184 551 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 551 457 245 184 67 NaeI GCC'GGC Cuts at: 2092 2092 Size: 5449 NarI GG'CG_CC Cuts at: 2085 2124 2085 Size: 39 5410 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4897 NdeI CA'TA_TG Cuts at: 387 387 Size: 5449 NgoMIV G'CCGG_C Cuts at: 2090 2090 Size: 5449 NheI G'CTAG_C Cuts at: 1052 2512 1052 Size: 1460 3989 NotI GC'GGCC_GC Cuts at: 2896 2896 Size: 5449 NspI r_CATG'y Cuts at: 1132 1229 2026 2524 2686 2807 4352 1132 Size: 97 797 498 162 121 1545 2229 Fragments arranged by size: 2229 1545 797 498 162 121 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5449 PciI A'CATG_T Cuts at: 2022 4348 2022 Size: 2326 3123 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5449 PmeI GTTT'AAAC Cuts at: 2753 2753 Size: 5449 PmlI CAC'GTG Cuts at: 2410 2410 Size: 5449 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3518 3550 5031 5063 3518 Size: 32 1481 32 3904 Fragments arranged by size: 3904 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2689 2689 Size: 5449 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5449 PsiI TTA'TAA Cuts at: 2565 3019 2565 Size: 454 4995 PstI C_TGCA'G Cuts at: 830 2306 2511 2801 830 Size: 1476 205 290 3478 Fragments arranged by size: 3478 1476 290 205 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5449 PvuII CAG'CTG Cuts at: 2314 2314 Size: 5449 SacII CC_GC'GG Cuts at: 2064 2064 Size: 5449 SalI G'TCGA_C Cuts at: 1936 2791 1936 Size: 855 4594 SapI GCTCTTCn'nnn_ Cuts at: 2256 2256 Size: 5449 SbfI CC_TGCA'GG Cuts at: 2801 2801 Size: 5449 ScaI AGT'ACT Cuts at: 3697 5409 3697 Size: 1712 3737 SfcI C'TryA_G Cuts at: 826 1045 2302 2507 2575 2797 3932 4613 4804 Size: 219 1257 205 68 222 1135 681 191 Cuts at: 4804 826 Size: 1471 Fragments arranged by size: 1471 1257 1135 681 222 219 205 191 68 SfoI GGC'GCC Cuts at: 2086 2125 2086 Size: 39 5410 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5449 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5449 SmaI CCC'GGG Cuts at: 1872 2776 1872 Size: 904 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3553 4454 4716 820 Size: 197 926 1610 901 262 1553 Fragments arranged by size: 1610 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3240 493 Size: 2747 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5449 SphI G_CATG'C Cuts at: 1132 2524 2807 1132 Size: 1392 283 3774 Fragments arranged by size: 3774 1392 283 SspI AAT'ATT Cuts at: 5 52 3373 5 Size: 47 3321 2081 Fragments arranged by size: 3321 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5449 StyI C'CwwG_G Cuts at: 513 1065 2888 513 Size: 552 1823 3074 Fragments arranged by size: 3074 1823 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2692 3476 3493 3646 3831 4276 325 Size: 1255 1112 784 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1112 784 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2630 3695 5407 Size: 274 80 33 51 1443 653 1065 1712 Cuts at: 5407 96 Size: 138 Fragments arranged by size: 1712 1443 1065 653 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2139 2171 5409 5441 2139 Size: 32 3238 32 2147 Fragments arranged by size: 3238 2147 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5449 XbaI T'CTAG_A Cuts at: 2785 2785 Size: 5449 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5449 XmaI C'CCGG_G Cuts at: 1870 2774 1870 Size: 904 4545 XmnI GAAnn'nnTTC Cuts at: 3578 3578 Size: 5449 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3257 276 Size: 53 83 186 936 1723 2468 Fragments arranged by size: 2468 1723 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SrfI SwaI XcmI