Restriction map for
pFN21AB3876
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# NO CUT ENZYMES ( 185 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
AspEI AspHI AvaII AviII AvrII
BamHI BanII BbeI BbrPI BbsI
BbvCI BbvI BcgI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaHI BsaI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DpnII
DraI DraII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRV EcoT14I
EcoT22I FauI FbaI Fnu4HI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
ItaI KasI Ksp632I KspI MaeI
MaeIII MamI MboI MfeI MflI
MluI MroI MslI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
Sau3AI Sau96I SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TaqI TfiI TseI
Tsp45I TspRI TthHB8I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 206 GGTACC G^GTACC
AciI 461 GCGG CCGC(-3/-1)
AcsI 445 GAATTC [GA]^AATT[CT]
AfaI 207 GTAC GT^AC
AlwNI 296 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 445 GAATTC [GA]^AATT[CT]
Asp718I 206 GGTACC G^GTACC
AspI 476 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 161 CCCGAG C^[CT]CG[GA]G
BalI 65 TGGCCA TGG^CCA
BciVI 224 GTATCC GTATCC(6/5)
BcnI 96 CCCGG CC^[GC]GG
BmyI 118 GTGCCC G[AGT]GC[ACT]^C
BsaAI 169 TACGTG [CT]AC^GT[GA]
BsaWI 20 ACCGGA [AT]^CCGG[AT]
BsmFI 159 GTCCC GGGAC(10/14)
BsoBI 161 CCCGAG C^[CT]CG[GA]G
Bsp1286I 118 GTGCCC G[AGT]GC[ACT]^C
BspHI 134 TCATGA T^CATGA
BspMI 339 ACCTGC ACCTGC(4/8)
DraIII 398 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 65 TGGCCA [CT]^GGCC[GA]
EcoNI 42 CCTTTTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 445 GAATTC G^AATTC
HphI 331 GGTGA GGTGA(8/7)
KpnI 206 GGTACC GGTAC^C
MboII 15 GAAGA GAAGA(8/7)
MluNI 65 TGGCCA TGG^CCA
MscI 65 TGGCCA TGG^CCA
MseI 47 TTAA T^TAA
NciI 96 CCCGG CC^[GC]GG
NspI 83 ACATGC [GA]CATG^[CT]
PflFI 476 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 295 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 134 TCATGA T^CATGA
RsaI 207 GTAC GT^AC
SexAI 399 ACCTGGT A^CC[AT]GGT
SfaNI 6 GATGC GCATC(5/9)
SfcI 378 CTACAG C^T[GA][CT]AG
SmlI 154 CTCAAG C^T[CT][GA]AG
StuI 307 AGGCCT AGG^CCT
Tru9I 47 TTAA T^TAA
Tsp509I 446 AATT ^AATT
Tth111I 476 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 295 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AluI 101 AGCT AG^CT
363 AGCT
BanI 206 GGTACC G^G[CT][GA]CC
403 GGTGCC
BpmI 189 CTGGAG CTGGAG(16/14)
325 CTGGAG
BstF5I 276 CATCC GGATG(2/0)
75 GGATG
BstNI 188 CCTGG CC^[AT]GG
400 CCTGG
EcoRII 188 CCTGG ^CC[AT]GG
400 CCTGG
FokI 276 CATCC GGATG(9/13)
75 GGATG
HapII 21 CCGG C^CGG
97 CCGG
HinfI 56 GAGTC G^A[ACGT]TC
347 GACTC
HpaII 21 CCGG C^CGG
97 CCGG
MaeII 170 ACGT A^CGT
477 ACGT
MspI 21 CCGG C^CGG
97 CCGG
MvaI 188 CCTGG CC^[AT]GG
400 CCTGG
MwoI 93 GCACCCGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
430 GCTGGGTCAGC
NlaIV 206 GGTACC GG[ACGT]^[ACGT]CC
403 GGTGCC
PleI 347 GACTC GAGTC(4/5)
56 GAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
#########################################
BsaJI BsiYI BslI BsmAI BsrI
BssKI Cac8I DdeI HaeIII MnlI
NlaIII ScrFI