(Circular) (Six-base) MAPSORT of: pFN21AB3618.seq Check: 9342 from: 1 to: 5590 pFN21AB3618 5590 bp With 182 enzymes: SgfI * October 20, 2009 10:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3400 278 Size: 53 83 186 936 1864 2468 Fragments arranged by size: 2468 1864 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2933 1937 Size: 996 4594 Acc65I G'GTAC_C Cuts at: 2911 2911 Size: 5590 AclI AA'CG_TT Cuts at: 1563 3717 4090 1563 Size: 2154 373 3063 Fragments arranged by size: 3063 2154 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2114 2639 2693 3653 5037 5305 1632 Size: 482 525 54 960 1384 268 1917 Fragments arranged by size: 1917 1384 960 525 482 268 54 AfeI AGC'GCT Cuts at: 2726 2726 Size: 5590 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5393 AflIII A'CryG_T Cuts at: 1203 4489 5466 1203 Size: 3286 977 1327 Fragments arranged by size: 3286 1327 977 AgeI A'CCGG_T Cuts at: 4421 4421 Size: 5590 AhdI GACnn_n'nnGTC Cuts at: 4319 4319 Size: 5590 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5590 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5590 AlwNI CAG_nnn'CTG Cuts at: 3333 4905 3333 Size: 1572 4018 ApaI G_GGCC'C Cuts at: 2886 2886 Size: 5590 ApaLI G'TGCA_C Cuts at: 3647 4803 3647 Size: 1156 4434 ApoI r'AATT_y Cuts at: 1493 2899 3125 3277 5386 1493 Size: 1406 226 152 2109 1697 Fragments arranged by size: 2109 1697 1406 226 152 AseI AT'TA_AT Cuts at: 160 1032 4144 160 Size: 872 3112 1606 Fragments arranged by size: 3112 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5590 AvaI C'yCGr_G Cuts at: 1870 1943 2915 1870 Size: 73 972 4545 Fragments arranged by size: 4545 972 73 BamHI G'GATC_C Cuts at: 2920 3287 2920 Size: 367 5223 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2911 4366 618 Size: 325 217 627 1124 1455 1842 Fragments arranged by size: 1842 1455 1124 627 325 217 BanII G_rGCy'C Cuts at: 1391 2444 2886 2909 1391 Size: 1053 442 23 4072 Fragments arranged by size: 4072 1053 442 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2121 2647 928 Size: 971 222 526 3871 Fragments arranged by size: 3871 971 526 222 BbvCI CC'TCA_GC Cuts at: 2381 2381 Size: 5590 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2416 2450 3781 3815 1903 Size: 34 479 34 1331 34 3678 Fragments arranged by size: 3678 1331 479 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3482 4692 3482 Size: 1210 4380 BclI T'GATC_A Cuts at: 1548 2634 1548 Size: 1086 4504 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2771 4201 136 Size: 107 122 71 631 605 1099 1430 1525 Fragments arranged by size: 1525 1430 1099 631 605 122 107 71 BglII A'GATC_T Cuts at: 2545 5585 2545 Size: 3040 2550 BlpI GC'TnA_GC Cuts at: 3007 3007 Size: 5590 Bme1580I G_kGCm'C Cuts at: 1790 2886 3651 4807 1790 Size: 1096 765 1156 2573 Fragments arranged by size: 2573 1156 1096 765 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5590 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2179 2513 2698 Size: 416 53 83 399 24 762 334 185 Cuts at: 2698 3321 4279 442 Size: 623 958 1753 Fragments arranged by size: 1753 958 762 623 416 399 334 185 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5590 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2102 2288 2804 4250 1239 Size: 863 186 516 1446 2579 Fragments arranged by size: 2579 1446 863 516 186 Bpu10I CC'TnA_GC Cuts at: 2069 2381 2069 Size: 312 5278 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2845 3715 4580 4878 2845 Size: 870 865 298 3557 Fragments arranged by size: 3557 870 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4253 882 Size: 617 2754 2219 Fragments arranged by size: 2754 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3381 493 Size: 711 2177 2702 Fragments arranged by size: 2702 2177 711 BsaBI GATnn'nnATC Cuts at: 2765 5584 2765 Size: 2819 2771 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2736 3397 3779 4414 Size: 53 83 186 936 1203 661 382 635 Cuts at: 4414 275 Size: 1451 Fragments arranged by size: 1451 1203 936 661 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4023 4421 4695 4842 5253 4023 Size: 398 274 147 411 4360 Fragments arranged by size: 4360 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2000 2030 2264 2294 1178 Size: 30 165 30 597 30 234 30 4474 Fragments arranged by size: 4474 597 234 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2285 1186 Size: 1099 4491 BseYI C'CCAG_C Cuts at: 2300 2539 2836 4793 5289 2300 Size: 239 297 1957 496 2601 Fragments arranged by size: 2601 1957 496 297 239 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2382 1163 Size: 573 646 4371 Fragments arranged by size: 4371 646 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2024 3040 3801 4829 1540 Size: 275 186 23 1016 761 1028 2301 Fragments arranged by size: 2301 1028 1016 761 275 186 23 BsiHKAI G_wGCw'C Cuts at: 2070 3651 3736 4807 2070 Size: 1581 85 1071 2853 Fragments arranged by size: 2853 1581 1071 85 BsmI GAATG_Cn' Cuts at: 2192 2292 3101 3194 2192 Size: 100 809 93 4588 Fragments arranged by size: 4588 809 100 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2444 2886 2909 3651 3736 4807 Size: 399 280 374 442 23 742 85 1071 Cuts at: 4807 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 442 399 374 280 85 23 BspEI T'CCGG_A Cuts at: 5253 5253 Size: 5590 BspHI T'CATG_A Cuts at: 3479 5209 3479 Size: 1730 3860 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2945 844 Size: 2101 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2026 3477 1394 Size: 429 203 1451 3507 Fragments arranged by size: 3507 1451 429 203 BsrDI GCAATG_nn' Cuts at: 65 1248 4085 4259 65 Size: 1183 2837 174 1396 Fragments arranged by size: 2837 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4234 4421 1700 Size: 110 2424 187 2869 Fragments arranged by size: 2869 2424 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5590 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5590 BssSI C'ACGA_G Cuts at: 3650 4662 3650 Size: 1012 4578 BstAPI GCAn_nnn'nTGC Cuts at: 794 2549 794 Size: 1755 3835 BstBI TT'CG_AA Cuts at: 4394 4394 Size: 5590 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5590 BstYI r'GATC_y Cuts at: 1972 2545 2920 3287 3672 3689 5141 5227 5239 Size: 573 375 367 385 17 1452 86 12 Cuts at: 5239 5585 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 573 385 375 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4099 Fragments arranged by size: 4099 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4851 Fragments arranged by size: 4851 683 56 BtsI GCAGTG_nn' Cuts at: 3106 3900 3920 3106 Size: 794 20 4776 Fragments arranged by size: 4776 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5590 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5555 DraI TTT'AAA Cuts at: 2894 2894 Size: 5590 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4597 809 Size: 507 3281 1802 Fragments arranged by size: 3281 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2569 3037 3926 Size: 54 1425 50 275 18 739 468 889 Cuts at: 3926 5555 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 739 468 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3037 1537 Size: 275 1225 4090 Fragments arranged by size: 4090 1225 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3520 1353 Size: 2167 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2795 4177 4551 4697 387 Size: 1064 138 1206 1382 374 146 1280 Fragments arranged by size: 1382 1280 1206 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2114 2288 2639 2693 2804 3653 Size: 393 470 12 174 351 54 111 849 Cuts at: 3653 4250 5037 5305 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 849 787 597 470 393 351 268 174 111 54 12 EcoNI CCTnn'n_nnAGG Cuts at: 2786 2807 2786 Size: 21 5569 EcoO109I rG'GnC_Cy Cuts at: 2073 2201 2515 2883 2073 Size: 128 314 368 4780 Fragments arranged by size: 4780 368 314 128 EcoRI G'AATT_C Cuts at: 2899 3277 2899 Size: 378 5212 FspI TGC'GCA Cuts at: 4096 4096 Size: 5590 HaeII r_GCGC'y Cuts at: 1678 2728 4737 1678 Size: 1050 2009 2531 Fragments arranged by size: 2531 2009 1050 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2000 2032 2264 2296 4231 Size: 32 135 32 428 32 232 32 1935 Cuts at: 4231 4263 4305 4337 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1935 428 232 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2934 3180 669 Size: 988 281 996 246 3079 Fragments arranged by size: 3079 996 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5590 HpaI GTT'AAC Cuts at: 3180 3180 Size: 5590 KpnI G_GTAC'C Cuts at: 2915 2915 Size: 5590 MfeI C'AATT_G Cuts at: 3189 3189 Size: 5590 MluI A'CGCG_T Cuts at: 5466 5466 Size: 5590 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2232 4704 4888 1305 Size: 927 2472 184 2007 Fragments arranged by size: 2472 2007 927 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2571 10 Size: 54 1425 343 739 3029 Fragments arranged by size: 3029 1425 739 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3548 3907 4066 518 Size: 648 2382 359 159 2042 Fragments arranged by size: 2382 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2092 2461 3006 3683 4831 5076 1606 Size: 486 369 545 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 545 486 369 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4099 Fragments arranged by size: 4099 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5590 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5590 NotI GC'GGCC_GC Cuts at: 3037 3037 Size: 5590 NspI r_CATG'y Cuts at: 1132 1229 2948 4493 1132 Size: 97 1719 1545 2229 Fragments arranged by size: 2229 1719 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5590 PciI A'CATG_T Cuts at: 4489 4489 Size: 5590 PflMI CCAn_nnn'nTGG Cuts at: 1108 2815 1108 Size: 1707 3883 PfoI T'CCnGG_A Cuts at: 2781 2781 Size: 5590 PmeI GTTT'AAAC Cuts at: 2894 2894 Size: 5590 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3659 3691 5172 5204 3659 Size: 32 1481 32 4045 Fragments arranged by size: 4045 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2073 2515 2073 Size: 442 5148 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5590 PsiI TTA'TAA Cuts at: 3160 3160 Size: 5590 PspOMI G'GGCC_C Cuts at: 2882 2882 Size: 5590 PstI C_TGCA'G Cuts at: 830 2346 2942 830 Size: 1516 596 3478 Fragments arranged by size: 3478 1516 596 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5590 PvuII CAG'CTG Cuts at: 2461 2461 Size: 5590 SalI G'TCGA_C Cuts at: 1936 2932 1936 Size: 996 4594 SbfI CC_TGCA'GG Cuts at: 2942 2942 Size: 5590 ScaI AGT'ACT Cuts at: 2687 3838 5550 2687 Size: 1151 1712 2727 Fragments arranged by size: 2727 1712 1151 SexAI A'CCwGG_T Cuts at: 2452 2452 Size: 5590 SfcI C'TryA_G Cuts at: 826 1045 2342 2938 4073 4754 4945 826 Size: 219 1297 596 1135 681 191 1471 Fragments arranged by size: 1471 1297 1135 681 596 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5590 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5590 SmaI CCC'GGG Cuts at: 1872 2917 1872 Size: 1045 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2824 3694 4595 4857 820 Size: 197 926 881 870 901 262 1553 Fragments arranged by size: 1553 926 901 881 870 262 197 SnaBI TAC'GTA Cuts at: 493 3381 493 Size: 2888 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5590 SphI G_CATG'C Cuts at: 1132 2948 1132 Size: 1816 3774 SspI AAT'ATT Cuts at: 5 52 3514 5 Size: 47 3462 2081 Fragments arranged by size: 3462 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5590 StyI C'CwwG_G Cuts at: 513 1065 2004 3029 513 Size: 552 939 1025 3074 Fragments arranged by size: 3074 1025 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2359 3617 3634 3787 3972 4417 325 Size: 1255 779 1258 17 153 185 445 1498 Fragments arranged by size: 1498 1258 1255 779 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2685 3836 5548 Size: 274 80 33 51 1443 708 1151 1712 Cuts at: 5548 96 Size: 138 Fragments arranged by size: 1712 1443 1151 708 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5550 5582 5550 Size: 32 5558 Tth111I GACn'n_nGTC Cuts at: 1316 2454 1316 Size: 1138 4452 XbaI T'CTAG_A Cuts at: 2926 2926 Size: 5590 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5590 XmaI C'CCGG_G Cuts at: 1870 2915 1870 Size: 1045 4545 XmnI GAAnn'nnTTC Cuts at: 3719 3719 Size: 5590 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3398 276 Size: 53 83 186 936 1864 2468 Fragments arranged by size: 2468 1864 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI