(Circular) (Six-base) MAPSORT of: pFN21AB9943.seq Check: 5770 from: 1 to: 6196 pFN21AB9943 6196 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4006 278 Size: 53 83 186 936 2470 2468 Fragments arranged by size: 2470 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2667 3539 1937 Size: 730 872 4594 Fragments arranged by size: 4594 872 730 Acc65I G'GTAC_C Cuts at: 3517 3517 Size: 6196 AclI AA'CG_TT Cuts at: 1563 4323 4696 1563 Size: 2760 373 3063 Fragments arranged by size: 3063 2760 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2127 2153 2284 2402 2708 4259 5643 5911 Size: 495 26 131 118 306 1551 1384 268 Cuts at: 5911 1632 Size: 1917 Fragments arranged by size: 1917 1551 1384 495 306 268 131 118 26 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5999 AflIII A'CryG_T Cuts at: 1203 5095 6072 1203 Size: 3892 977 1327 Fragments arranged by size: 3892 1327 977 AgeI A'CCGG_T Cuts at: 5027 5027 Size: 6196 AhdI GACnn_n'nnGTC Cuts at: 4925 4925 Size: 6196 AleI CACnn'nnGTG Cuts at: 1166 3002 1166 Size: 1836 4360 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6196 AlwNI CAG_nnn'CTG Cuts at: 3428 3939 5511 3428 Size: 511 1572 4113 Fragments arranged by size: 4113 1572 511 ApaLI G'TGCA_C Cuts at: 4253 5409 4253 Size: 1156 5040 ApoI r'AATT_y Cuts at: 1493 2151 2210 2231 2717 2967 3242 3315 3505 Size: 658 59 21 486 250 275 73 190 Cuts at: 3505 3731 3883 5992 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 658 486 275 250 226 190 152 73 59 21 AseI AT'TA_AT Cuts at: 160 1032 4750 160 Size: 872 3718 1606 Fragments arranged by size: 3718 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6196 AvaI C'yCGr_G Cuts at: 1870 1943 3521 1870 Size: 73 1578 4545 Fragments arranged by size: 4545 1578 73 BamHI G'GATC_C Cuts at: 3526 3893 3526 Size: 367 5829 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2747 3517 4972 618 Size: 325 217 627 960 770 1455 1842 Fragments arranged by size: 1842 1455 960 770 627 325 217 BanII G_rGCy'C Cuts at: 1391 3515 1391 Size: 2124 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2564 928 Size: 971 665 4560 Fragments arranged by size: 4560 971 665 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4387 4421 1903 Size: 34 2450 34 3678 Fragments arranged by size: 3678 2450 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4088 5298 4088 Size: 1210 4986 BclI T'GATC_A Cuts at: 1548 2764 1548 Size: 1216 4980 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4807 136 Size: 107 122 71 631 605 3135 1525 Fragments arranged by size: 3135 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6191 6191 Size: 6196 BlpI GC'TnA_GC Cuts at: 3613 3613 Size: 6196 Bme1580I G_kGCm'C Cuts at: 1790 4257 5413 1790 Size: 2467 1156 2573 Fragments arranged by size: 2573 2467 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6196 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3124 3927 4885 Size: 416 53 83 399 24 1707 803 958 Cuts at: 4885 442 Size: 1753 Fragments arranged by size: 1753 1707 958 803 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6196 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3490 4856 1239 Size: 2251 1366 2579 Fragments arranged by size: 2579 2251 1366 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2635 4321 5186 5484 2635 Size: 1686 865 298 3347 Fragments arranged by size: 3347 1686 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4859 882 Size: 617 3360 2219 Fragments arranged by size: 3360 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3987 493 Size: 711 2783 2702 Fragments arranged by size: 2783 2702 711 BsaBI GATnn'nnATC Cuts at: 2763 6190 2763 Size: 3427 2769 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2105 4003 4385 5020 Size: 53 83 186 936 572 1898 382 635 Cuts at: 5020 275 Size: 1451 Fragments arranged by size: 1898 1451 936 635 572 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4629 5027 5301 5448 5859 4629 Size: 398 274 147 411 4966 Fragments arranged by size: 4966 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5971 Fragments arranged by size: 5971 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6196 BseYI C'CCAG_C Cuts at: 3219 5399 5895 3219 Size: 2180 496 3520 Fragments arranged by size: 3520 2180 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5623 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3646 4407 5435 1540 Size: 275 186 1645 761 1028 2301 Fragments arranged by size: 2301 1645 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4257 4342 5413 4257 Size: 85 1071 5040 Fragments arranged by size: 5040 1071 85 BsmI GAATG_Cn' Cuts at: 3707 3800 3707 Size: 93 6103 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3515 4257 4342 5413 1391 Size: 399 1725 742 85 1071 2174 Fragments arranged by size: 2174 1725 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5859 5859 Size: 6196 BspHI T'CATG_A Cuts at: 4085 5815 4085 Size: 1730 4466 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3551 844 Size: 2707 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4083 1394 Size: 429 2260 3507 Fragments arranged by size: 3507 2260 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4691 4865 65 Size: 1183 3443 174 1396 Fragments arranged by size: 3443 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2090 4840 5027 1700 Size: 110 280 2750 187 2869 Fragments arranged by size: 2869 2750 280 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6196 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6196 BssSI C'ACGA_G Cuts at: 4256 5268 4256 Size: 1012 5184 BstAPI GCAn_nnn'nTGC Cuts at: 794 2317 794 Size: 1523 4673 BstBI TT'CG_AA Cuts at: 5000 5000 Size: 6196 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6196 BstYI r'GATC_y Cuts at: 1972 3358 3526 3893 4278 4295 5747 5833 5845 Size: 1386 168 367 385 17 1452 86 12 Cuts at: 5845 6191 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1386 385 367 346 168 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2046 513 Size: 552 939 42 4663 Fragments arranged by size: 4663 939 552 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5457 Fragments arranged by size: 5457 683 56 BtsI GCAGTG_nn' Cuts at: 3712 4506 4526 3712 Size: 794 20 5382 Fragments arranged by size: 5382 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6196 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6161 DraI TTT'AAA Cuts at: 2236 2512 3500 2236 Size: 276 988 4932 Fragments arranged by size: 4932 988 276 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5203 809 Size: 507 3887 1802 Fragments arranged by size: 3887 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2040 2079 Size: 54 1425 50 275 18 177 33 39 Cuts at: 2079 2388 3643 4532 6161 8 Size: 309 1255 889 1629 43 Fragments arranged by size: 1629 1425 1255 889 309 275 177 54 50 43 39 33 18 EagI C'GGCC_G Cuts at: 1537 1812 3643 1537 Size: 275 1831 4090 Fragments arranged by size: 4090 1831 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2511 2532 2547 2691 2694 3141 3484 4126 Size: 1158 21 15 144 3 447 343 642 Cuts at: 4126 1353 Size: 3423 Fragments arranged by size: 3423 1158 642 447 343 144 21 15 3 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4783 5157 5303 387 Size: 1064 138 3194 374 146 1280 Fragments arranged by size: 3194 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2127 2153 2284 2402 2708 3490 4259 Size: 393 495 26 131 118 306 782 769 Cuts at: 4259 4856 5643 5911 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 782 769 597 495 393 306 268 131 118 26 EcoRI G'AATT_C Cuts at: 2151 3505 3883 2151 Size: 1354 378 4464 Fragments arranged by size: 4464 1354 378 FspI TGC'GCA Cuts at: 4702 4702 Size: 6196 HaeII r_GCGC'y Cuts at: 1678 2114 3184 5343 1678 Size: 436 1070 2159 2531 Fragments arranged by size: 2531 2159 1070 436 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2358 2390 4837 4869 4911 Size: 32 135 32 786 32 2447 32 42 Cuts at: 4911 4943 1373 Size: 32 2626 Fragments arranged by size: 2626 2447 786 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3540 3786 669 Size: 988 281 1602 246 3079 Fragments arranged by size: 3079 1602 988 281 246 HindIII A'AGCT_T Cuts at: 748 2634 3081 748 Size: 1886 447 3863 Fragments arranged by size: 3863 1886 447 HpaI GTT'AAC Cuts at: 3786 3786 Size: 6196 KpnI G_GTAC'C Cuts at: 3521 3521 Size: 6196 MfeI C'AATT_G Cuts at: 2384 3795 2384 Size: 1411 4785 MluI A'CGCG_T Cuts at: 6072 6072 Size: 6196 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3488 5310 5494 1305 Size: 2183 1822 184 2007 Fragments arranged by size: 2183 2007 1822 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2390 10 Size: 54 1425 343 558 3816 Fragments arranged by size: 3816 1425 558 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2403 3002 4154 4513 4672 518 Size: 648 1237 599 1152 359 159 2042 Fragments arranged by size: 2042 1237 1152 648 599 359 159 MspA1I CmG'CkG Cuts at: 1606 2048 3612 4289 5437 5682 1606 Size: 442 1564 677 1148 245 2120 Fragments arranged by size: 2120 1564 1148 677 442 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4705 Fragments arranged by size: 4705 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6196 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6196 NotI GC'GGCC_GC Cuts at: 3643 3643 Size: 6196 NspI r_CATG'y Cuts at: 1132 1229 3554 5099 1132 Size: 97 2325 1545 2229 Fragments arranged by size: 2325 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6196 PciI A'CATG_T Cuts at: 5095 5095 Size: 6196 PflMI CCAn_nnn'nTGG Cuts at: 1108 3390 1108 Size: 2282 3914 PfoI T'CCnGG_A Cuts at: 2842 2842 Size: 6196 PmeI GTTT'AAAC Cuts at: 3500 3500 Size: 6196 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4265 4297 5778 5810 4265 Size: 32 1481 32 4651 Fragments arranged by size: 4651 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6196 PsiI TTA'TAA Cuts at: 3766 3766 Size: 6196 PstI C_TGCA'G Cuts at: 830 3548 830 Size: 2718 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6196 SacII CC_GC'GG Cuts at: 2049 2049 Size: 6196 SalI G'TCGA_C Cuts at: 1936 3538 1936 Size: 1602 4594 SapI GCTCTTCn'nnn_ Cuts at: 2511 2532 2511 Size: 21 6175 SbfI CC_TGCA'GG Cuts at: 3548 3548 Size: 6196 ScaI AGT'ACT Cuts at: 2140 4444 6156 2140 Size: 2304 1712 2180 Fragments arranged by size: 2304 2180 1712 SfcI C'TryA_G Cuts at: 826 1045 2664 3544 4679 5360 5551 826 Size: 219 1619 880 1135 681 191 1471 Fragments arranged by size: 1619 1471 1135 880 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6196 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6196 SmaI CCC'GGG Cuts at: 1872 3523 1872 Size: 1651 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2650 4300 5201 5463 820 Size: 197 926 707 1650 901 262 1553 Fragments arranged by size: 1650 1553 926 901 707 262 197 SnaBI TAC'GTA Cuts at: 493 3987 493 Size: 3494 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6196 SphI G_CATG'C Cuts at: 1132 3554 1132 Size: 2422 3774 SspI AAT'ATT Cuts at: 5 52 4120 5 Size: 47 4068 2081 Fragments arranged by size: 4068 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6196 StyI C'CwwG_G Cuts at: 513 1065 2004 3635 513 Size: 552 939 1631 3074 Fragments arranged by size: 3074 1631 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4223 4240 4393 4578 5023 325 Size: 1255 2643 17 153 185 445 1498 Fragments arranged by size: 2643 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2138 4442 6154 Size: 274 80 33 51 1443 161 2304 1712 Cuts at: 6154 96 Size: 138 Fragments arranged by size: 2304 1712 1443 274 161 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6156 6188 6156 Size: 32 6164 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6196 XbaI T'CTAG_A Cuts at: 2830 2902 3532 2830 Size: 72 630 5494 Fragments arranged by size: 5494 630 72 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6196 XmaI C'CCGG_G Cuts at: 1870 3521 1870 Size: 1651 4545 XmnI GAAnn'nnTTC Cuts at: 2636 2826 2838 4325 2636 Size: 190 12 1487 4507 Fragments arranged by size: 4507 1487 190 12 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4004 276 Size: 53 83 186 936 2470 2468 Fragments arranged by size: 2470 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI XcmI