(Circular) (Six-base) MAPSORT of: pFN21AB6206.seq Check: 5898 from: 1 to: 6850 pFN21AB6206 6850 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4660 278 Size: 53 83 186 936 3124 2468 Fragments arranged by size: 3124 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4193 1937 Size: 2256 4594 Acc65I G'GTAC_C Cuts at: 4171 4171 Size: 6850 AclI AA'CG_TT Cuts at: 1563 2703 4977 5350 1563 Size: 1140 2274 373 3063 Fragments arranged by size: 3063 2274 1140 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2824 2965 3322 3406 3658 3742 4913 6297 Size: 1192 141 357 84 252 84 1171 1384 Cuts at: 6297 6565 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1192 1171 357 268 252 141 84 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6653 AflIII A'CryG_T Cuts at: 1203 5749 6726 1203 Size: 4546 977 1327 Fragments arranged by size: 4546 1327 977 AgeI A'CCGG_T Cuts at: 5681 5681 Size: 6850 AhdI GACnn_n'nnGTC Cuts at: 5579 5579 Size: 6850 AleI CACnn'nnGTG Cuts at: 1166 3969 1166 Size: 2803 4047 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2062 1184 Size: 878 5972 AlwNI CAG_nnn'CTG Cuts at: 4593 6165 4593 Size: 1572 5278 ApaLI G'TGCA_C Cuts at: 3400 4907 6063 3400 Size: 1507 1156 4187 Fragments arranged by size: 4187 1507 1156 ApoI r'AATT_y Cuts at: 1493 2281 2593 3288 3372 3624 3708 3876 4044 Size: 788 312 695 84 252 84 168 168 Cuts at: 4044 4159 4385 4537 6646 1493 Size: 115 226 152 2109 1697 Fragments arranged by size: 2109 1697 788 695 312 252 226 168 168 152 115 84 84 AseI AT'TA_AT Cuts at: 160 1032 2607 5404 160 Size: 872 1575 2797 1606 Fragments arranged by size: 2797 1606 1575 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6850 AvaI C'yCGr_G Cuts at: 1870 1943 2244 4175 1870 Size: 73 301 1931 4545 Fragments arranged by size: 4545 1931 301 73 BamHI G'GATC_C Cuts at: 4180 4547 4180 Size: 367 6483 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4171 5626 618 Size: 325 217 627 2384 1455 1842 Fragments arranged by size: 2384 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3938 4022 4169 1391 Size: 2547 84 147 4072 Fragments arranged by size: 4072 2547 147 84 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5879 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5041 5075 1903 Size: 34 3104 34 3678 Fragments arranged by size: 3678 3104 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4742 5952 4742 Size: 1210 5640 BclI T'GATC_A Cuts at: 1548 1548 Size: 6850 BfrBI ATG'CAT Cuts at: 3952 3952 Size: 6850 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5461 136 Size: 107 122 71 631 605 3789 1525 Fragments arranged by size: 3789 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6845 6845 Size: 6850 BlpI GC'TnA_GC Cuts at: 4267 4267 Size: 6850 Bme1580I G_kGCm'C Cuts at: 1790 3404 4911 6067 1790 Size: 1614 1507 1156 2573 Fragments arranged by size: 2573 1614 1507 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6850 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3894 4581 5539 Size: 416 53 83 399 24 2477 687 958 Cuts at: 5539 442 Size: 1753 Fragments arranged by size: 2477 1753 958 687 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6850 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2374 2374 Size: 6850 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2222 2988 3150 3234 3318 3402 3486 Size: 917 66 766 162 84 84 84 84 Cuts at: 3486 3570 3654 3738 3822 4074 4158 5510 1239 Size: 84 84 84 84 252 84 1352 2579 Fragments arranged by size: 2579 1352 917 766 252 162 84 84 84 84 84 84 84 84 84 66 Bpu10I CC'TnA_GC Cuts at: 2559 2559 Size: 6850 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2285 2443 2707 4975 5840 6138 2285 Size: 158 264 2268 865 298 2997 Fragments arranged by size: 2997 2268 865 298 264 158 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2132 2966 5513 882 Size: 617 633 834 2547 2219 Fragments arranged by size: 2547 2219 834 633 617 BsaAI yAC'GTr Cuts at: 493 1204 2323 4641 493 Size: 711 1119 2318 2702 Fragments arranged by size: 2702 2318 1119 711 BsaBI GATnn'nnATC Cuts at: 6844 6844 Size: 6850 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4657 5039 5674 275 Size: 53 83 186 936 3124 382 635 1451 Fragments arranged by size: 3124 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2344 5283 5681 5955 6102 6513 2344 Size: 2939 398 274 147 411 2681 Fragments arranged by size: 2939 2681 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2261 2291 3881 3911 1178 Size: 30 165 30 858 30 1590 30 4117 Fragments arranged by size: 4117 1590 858 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2096 2282 2363 1186 Size: 910 186 81 5673 Fragments arranged by size: 5673 910 186 81 BseYI C'CCAG_C Cuts at: 6053 6549 6053 Size: 496 6354 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2242 2932 1163 Size: 573 506 690 5081 Fragments arranged by size: 5081 690 573 506 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4300 5061 6089 1540 Size: 275 186 2299 761 1028 2301 Fragments arranged by size: 2301 2299 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3404 3938 4022 4911 4996 6067 3404 Size: 534 84 889 85 1071 4187 Fragments arranged by size: 4187 1071 889 534 85 84 BsiWI C'GTAC_G Cuts at: 3729 3729 Size: 6850 BsmI GAATG_Cn' Cuts at: 2069 3329 4361 4454 2069 Size: 1260 1032 93 4465 Fragments arranged by size: 4465 1260 1032 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3404 3938 4022 4169 4911 4996 6067 Size: 399 1614 534 84 147 742 85 1071 Cuts at: 6067 1391 Size: 2174 Fragments arranged by size: 2174 1614 1071 742 534 399 147 85 84 BspEI T'CCGG_A Cuts at: 6513 6513 Size: 6850 BspHI T'CATG_A Cuts at: 2611 4739 6469 2611 Size: 2128 1730 2992 Fragments arranged by size: 2992 2128 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4205 844 Size: 3361 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4737 1394 Size: 429 2914 3507 Fragments arranged by size: 3507 2914 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5345 5519 65 Size: 1183 4097 174 1396 Fragments arranged by size: 4097 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5494 5681 1700 Size: 110 3684 187 2869 Fragments arranged by size: 3684 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2331 2944 3361 3529 96 Size: 2235 613 417 168 3417 Fragments arranged by size: 3417 2235 613 417 168 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6850 BssSI C'ACGA_G Cuts at: 4910 5922 4910 Size: 1012 5838 BstAPI GCAn_nnn'nTGC Cuts at: 794 3912 794 Size: 3118 3732 BstBI TT'CG_AA Cuts at: 3144 5654 3144 Size: 2510 4340 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6850 BstYI r'GATC_y Cuts at: 1972 3263 4180 4547 4932 4949 6401 6487 6499 Size: 1291 917 367 385 17 1452 86 12 Cuts at: 6499 6845 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1291 917 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3012 513 Size: 552 939 1008 4351 Fragments arranged by size: 4351 1008 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6111 Fragments arranged by size: 6111 683 56 BtsI GCAGTG_nn' Cuts at: 4366 5160 5180 4366 Size: 794 20 6036 Fragments arranged by size: 6036 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6850 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2343 2378 3559 3594 529 Size: 35 1779 35 1181 35 3785 Fragments arranged by size: 3785 1779 1181 35 35 35 DraI TTT'AAA Cuts at: 4154 4154 Size: 6850 DraIII CAC_nnn'GTG Cuts at: 2323 2851 2323 Size: 528 6322 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5857 809 Size: 507 4541 1802 Fragments arranged by size: 4541 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4297 5186 6815 Size: 54 1425 50 275 18 2467 889 1629 Cuts at: 6815 8 Size: 43 Fragments arranged by size: 2467 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4297 1537 Size: 275 2485 4090 Fragments arranged by size: 4090 2485 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2205 2843 4780 1353 Size: 852 638 1937 3423 Fragments arranged by size: 3423 1937 852 638 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4141 5437 5811 5957 387 Size: 1064 138 2552 1296 374 146 1280 Fragments arranged by size: 2552 1296 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3936 4020 3936 Size: 84 6766 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2222 2824 2965 2988 3150 3234 Size: 393 524 66 602 141 23 162 84 Cuts at: 3234 3318 3322 3402 3406 3486 3570 3654 3658 Size: 84 4 80 4 80 84 84 4 Cuts at: 3658 3738 3742 3822 4074 4158 4913 5510 6297 Size: 80 4 80 252 84 755 597 787 Cuts at: 6297 6565 1239 Size: 268 1524 Fragments arranged by size: 1524 787 755 602 597 524 393 268 252 162 141 84 84 84 84 84 80 80 80 80 66 23 4 4 4 4 EcoNI CCTnn'n_nnAGG Cuts at: 3928 3928 Size: 6850 EcoO109I rG'GnC_Cy Cuts at: 2215 2239 2215 Size: 24 6826 EcoRI G'AATT_C Cuts at: 2281 3288 3372 3624 3708 4044 4159 4537 2281 Size: 1007 84 252 84 336 115 378 4594 Fragments arranged by size: 4594 1007 378 336 252 115 84 84 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2279 2279 Size: 6850 FspI TGC'GCA Cuts at: 5356 5356 Size: 6850 HaeII r_GCGC'y Cuts at: 1678 5997 1678 Size: 4319 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2177 2209 3047 3079 5491 Size: 32 135 32 605 32 838 32 2412 Cuts at: 5491 5523 5565 5597 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2412 838 605 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4194 4440 669 Size: 988 281 2256 246 3079 Fragments arranged by size: 3079 2256 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6850 HpaI GTT'AAC Cuts at: 4440 4440 Size: 6850 KpnI G_GTAC'C Cuts at: 4175 4175 Size: 6850 MfeI C'AATT_G Cuts at: 4449 4449 Size: 6850 MluI A'CGCG_T Cuts at: 6726 6726 Size: 6850 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5964 6148 1305 Size: 4659 184 2007 Fragments arranged by size: 4659 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5028 Fragments arranged by size: 5028 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2044 2655 3067 3969 4808 5167 5326 Size: 648 878 611 412 902 839 359 159 Cuts at: 5326 518 Size: 2042 Fragments arranged by size: 2042 902 878 839 648 611 412 359 159 MspA1I CmG'CkG Cuts at: 1606 2095 2200 3096 4266 4943 6091 6336 1606 Size: 489 105 896 1170 677 1148 245 2120 Fragments arranged by size: 2120 1170 1148 896 677 489 245 105 NcoI C'CATG_G Cuts at: 513 1065 2004 3012 513 Size: 552 939 1008 4351 Fragments arranged by size: 4351 1008 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6850 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6850 NotI GC'GGCC_GC Cuts at: 4297 4297 Size: 6850 NsiI A_TGCA'T Cuts at: 3954 3954 Size: 6850 NspI r_CATG'y Cuts at: 1132 1229 4208 5753 1132 Size: 97 2979 1545 2229 Fragments arranged by size: 2979 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2161 1348 Size: 813 6037 PciI A'CATG_T Cuts at: 5749 5749 Size: 6850 PflMI CCAn_nnn'nTGG Cuts at: 1108 2323 1108 Size: 1215 5635 PmeI GTTT'AAAC Cuts at: 4154 4154 Size: 6850 PmlI CAC'GTG Cuts at: 2323 2323 Size: 6850 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4919 4951 6432 6464 4919 Size: 32 1481 32 5305 Fragments arranged by size: 5305 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2215 2215 Size: 6850 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6850 PsiI TTA'TAA Cuts at: 4420 4420 Size: 6850 PstI C_TGCA'G Cuts at: 830 4202 830 Size: 3372 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6850 PvuII CAG'CTG Cuts at: 2095 2200 3096 2095 Size: 105 896 5849 Fragments arranged by size: 5849 896 105 SacI G_AGCT'C Cuts at: 3938 4022 3938 Size: 84 6766 SalI G'TCGA_C Cuts at: 1936 4192 1936 Size: 2256 4594 SbfI CC_TGCA'GG Cuts at: 4202 4202 Size: 6850 ScaI AGT'ACT Cuts at: 5098 6810 5098 Size: 1712 5138 SfcI C'TryA_G Cuts at: 826 1045 3051 4198 5333 6014 6205 826 Size: 219 2006 1147 1135 681 191 1471 Fragments arranged by size: 2006 1471 1147 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6850 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6850 SmaI CCC'GGG Cuts at: 1872 4177 1872 Size: 2305 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2264 2422 2686 4954 5855 6117 Size: 197 926 321 158 264 2268 901 262 Cuts at: 6117 820 Size: 1553 Fragments arranged by size: 2268 1553 926 901 321 264 262 197 158 SnaBI TAC'GTA Cuts at: 493 4641 493 Size: 4148 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6850 SphI G_CATG'C Cuts at: 1132 4208 1132 Size: 3076 3774 SspI AAT'ATT Cuts at: 5 52 4774 5 Size: 47 4722 2081 Fragments arranged by size: 4722 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6850 StyI C'CwwG_G Cuts at: 513 1065 2004 3012 4289 513 Size: 552 939 1008 1277 3074 Fragments arranged by size: 3074 1277 1008 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3272 4877 4894 5047 5232 5677 325 Size: 1255 1692 1605 17 153 185 445 1498 Fragments arranged by size: 1692 1605 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2331 2546 2944 Size: 274 80 33 51 1443 354 215 398 Cuts at: 2944 3149 3361 3485 3529 5096 6808 96 Size: 205 212 124 44 1567 1712 138 Fragments arranged by size: 1712 1567 1443 398 354 274 215 212 205 138 124 80 51 44 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2626 2658 6810 6842 2626 Size: 32 4152 32 2634 Fragments arranged by size: 4152 2634 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3188 1316 Size: 1872 4978 XbaI T'CTAG_A Cuts at: 4186 4186 Size: 6850 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6850 XmaI C'CCGG_G Cuts at: 1870 4175 1870 Size: 2305 4545 XmnI GAAnn'nnTTC Cuts at: 2794 3251 3503 3587 3671 4048 4091 4979 2794 Size: 457 252 84 84 377 43 888 4665 Fragments arranged by size: 4665 888 457 377 252 84 84 43 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4658 276 Size: 53 83 186 936 3124 2468 Fragments arranged by size: 3124 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI