Restriction map for
pFN21AB6206
(Linear) (Six-base) MAPSORT of: KIBB6206.seq Check: 2785 from: 1 to: 2145
KIBB6206.orf
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AclI AA'CG_TT
Cuts at: 0 698 2145
Size: 698 1447
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 819 960 1317 1401 1653 1737 2145
Size: 819 141 357 84 252 84 408
Fragments arranged by size:
819 408 357 252 141 84 84
AleI CACnn'nnGTG
Cuts at: 0 1964 2145
Size: 1964 181
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 57 2145
Size: 57 2088
ApaLI G'TGCA_C
Cuts at: 0 1395 2145
Size: 1395 750
ApoI r'AATT_y
Cuts at: 0 276 588 1283 1367 1619 1703 1871 2039
Size: 276 312 695 84 252 84 168 168
Cuts at: 2039 2145
Size: 106
Fragments arranged by size:
695 312 276 252 168 168 106 84
84
AseI AT'TA_AT
Cuts at: 0 602 2145
Size: 602 1543
AvaI C'yCGr_G
Cuts at: 0 239 2145
Size: 239 1906
BanII G_rGCy'C
Cuts at: 0 1933 2017 2145
Size: 1933 84 128
Fragments arranged by size:
1933 128 84
BfrBI ATG'CAT
Cuts at: 0 1947 2145
Size: 1947 198
Bme1580I G_kGCm'C
Cuts at: 0 1399 2145
Size: 1399 746
BmrI ACTGGGnnnn_n'
Cuts at: 0 1889 2145
Size: 1889 256
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 369 2145
Size: 369 1776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 217 983 1145 1229 1313 1397 1481
Size: 151 66 766 162 84 84 84 84
Cuts at: 1481 1565 1649 1733 1817 2069 2145
Size: 84 84 84 84 252 76
Fragments arranged by size:
766 252 162 151 84 84 84 84
84 84 84 84 76 66
Bpu10I CC'TnA_GC
Cuts at: 0 554 2145
Size: 554 1591
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 438 702 2145
Size: 280 158 264 1443
Fragments arranged by size:
1443 280 264 158
BsaI GGTCTCn'nnnn_
Cuts at: 0 127 961 2145
Size: 127 834 1184
Fragments arranged by size:
1184 834 127
BsaAI yAC'GTr
Cuts at: 0 318 2145
Size: 318 1827
BsaWI w'CCGG_w
Cuts at: 0 339 2145
Size: 339 1806
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 256 286 1876 1906 2145
Size: 256 30 1590 30 239
Fragments arranged by size:
1590 256 239 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 277 358 2145
Size: 91 186 81 1787
Fragments arranged by size:
1787 186 91 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 927 2145
Size: 237 690 1218
Fragments arranged by size:
1218 690 237
BsiHKAI G_wGCw'C
Cuts at: 0 1399 1933 2017 2145
Size: 1399 534 84 128
Fragments arranged by size:
1399 534 128 84
BsiWI C'GTAC_G
Cuts at: 0 1724 2145
Size: 1724 421
BsmI GAATG_Cn'
Cuts at: 0 64 1324 2145
Size: 64 1260 821
Fragments arranged by size:
1260 821 64
Bsp1286I G_dGCh'C
Cuts at: 0 1399 1933 2017 2145
Size: 1399 534 84 128
Fragments arranged by size:
1399 534 128 84
BspHI T'CATG_A
Cuts at: 0 606 2145
Size: 606 1539
BsrGI T'GTAC_A
Cuts at: 0 326 939 1356 1524 2145
Size: 326 613 417 168 621
Fragments arranged by size:
621 613 417 326 168
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1907 2145
Size: 1907 238
BstBI TT'CG_AA
Cuts at: 0 1139 2145
Size: 1139 1006
BstYI r'GATC_y
Cuts at: 0 1258 2145
Size: 1258 887
BtgI C'CryG_G
Cuts at: 0 1007 2145
Size: 1007 1138
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 338 373 1554 1589 2145
Size: 338 35 1181 35 556
Fragments arranged by size:
1181 556 338 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 318 846 2145
Size: 318 528 1299
Fragments arranged by size:
1299 528 318
EarI CTCTTCn'nnn_
Cuts at: 0 200 838 2145
Size: 200 638 1307
Fragments arranged by size:
1307 638 200
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2136 2145
Size: 2136 9
EcoICRI GAG'CTC
Cuts at: 0 1931 2015 2145
Size: 1931 84 130
Fragments arranged by size:
1931 130 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 217 819 960 983 1145 1229 1313
Size: 151 66 602 141 23 162 84 84
Cuts at: 1313 1317 1397 1401 1481 1565 1649 1653 1733
Size: 4 80 4 80 84 84 4 80
Cuts at: 1733 1737 1817 2069 2145
Size: 4 80 252 76
Fragments arranged by size:
602 252 162 151 141 84 84 84
84 80 80 80 80 76 66 23
4 4 4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1923 2145
Size: 1923 222
EcoO109I rG'GnC_Cy
Cuts at: 0 210 234 2145
Size: 210 24 1911
Fragments arranged by size:
1911 210 24
EcoRI G'AATT_C
Cuts at: 0 276 1283 1367 1619 1703 2039 2145
Size: 276 1007 84 252 84 336 106
Fragments arranged by size:
1007 336 276 252 106 84 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 274 2145
Size: 274 1871
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 172 204 1042 1074 2145
Size: 172 32 838 32 1071
Fragments arranged by size:
1071 838 172 32 32
MslI CAynn'nnrTG
Cuts at: 0 39 650 1062 1964 2145
Size: 39 611 412 902 181
Fragments arranged by size:
902 611 412 181 39
MspA1I CmG'CkG
Cuts at: 0 90 195 1091 2145
Size: 90 105 896 1054
Fragments arranged by size:
1054 896 105 90
NcoI C'CATG_G
Cuts at: 0 1007 2145
Size: 1007 1138
NsiI A_TGCA'T
Cuts at: 0 1949 2145
Size: 1949 196
PasI CC'CwG_GG
Cuts at: 0 156 2145
Size: 156 1989
PflMI CCAn_nnn'nTGG
Cuts at: 0 318 2145
Size: 318 1827
PmlI CAC'GTG
Cuts at: 0 318 2145
Size: 318 1827
PpuMI rG'GwC_Cy
Cuts at: 0 210 2145
Size: 210 1935
PvuII CAG'CTG
Cuts at: 0 90 195 1091 2145
Size: 90 105 896 1054
Fragments arranged by size:
1054 896 105 90
SacI G_AGCT'C
Cuts at: 0 1933 2017 2145
Size: 1933 84 128
Fragments arranged by size:
1933 128 84
SfcI C'TryA_G
Cuts at: 0 1046 2145
Size: 1046 1099
SmlI C'TyrA_G
Cuts at: 0 259 417 681 2145
Size: 259 158 264 1464
Fragments arranged by size:
1464 264 259 158
StyI C'CwwG_G
Cuts at: 0 1007 2145
Size: 1007 1138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1267 2145
Size: 1267 878
TatI w'GTAC_w
Cuts at: 0 326 541 939 1144 1356 1480 1524 2145
Size: 326 215 398 205 212 124 44 621
Fragments arranged by size:
621 398 326 215 212 205 124 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 621 653 2145
Size: 621 32 1492
Fragments arranged by size:
1492 621 32
Tth111I GACn'n_nGTC
Cuts at: 0 1183 2145
Size: 1183 962
XmnI GAAnn'nnTTC
Cuts at: 0 789 1246 1498 1582 1666 2043 2086 2145
Size: 789 457 252 84 84 377 43 59
Fragments arranged by size:
789 457 377 252 84 84 59 43
Enzymes that do cut:
AclI AcuI AleI AlfI ApaLI ApoI AseI AvaI
BanII BfrBI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaWI BsaXI BseRI BsgI BsiHKAI BsiWI
BsmI Bsp1286I BspHI BsrGI BstAPI BstBI BstYI BtgI
CspCI DraIII EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI Hin4I MslI MspA1I NcoI NsiI PasI
PflMI PmlI PpuMI PvuII SacI SfcI SmlI StyI
TaqII TatI TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AloI AlwNI ApaI AscI AsiSI AvrII BaeI
BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI BmgBI BmtI BsaBI BsaHI BseYI
BsiEI BsmBI BspEI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI
DraI DrdI EaeI EagI EcoRV FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI NspI PacI PciI PfoI PmeI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI XbaI XcmI XhoI XmaI ZraI