(Circular) (Six-base) MAPSORT of: pFN21AB5235.seq Check: 2097 from: 1 to: 6232 pFN21AB5235 6232 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4042 278 Size: 53 83 186 936 2506 2468 Fragments arranged by size: 2506 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2718 3575 1937 Size: 781 857 4594 Fragments arranged by size: 4594 857 781 Acc65I G'GTAC_C Cuts at: 3553 3553 Size: 6232 AclI AA'CG_TT Cuts at: 1563 4359 4732 1563 Size: 2796 373 3063 Fragments arranged by size: 3063 2796 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2027 2147 2375 2594 2600 2930 4295 5679 Size: 395 120 228 219 6 330 1365 1384 Cuts at: 5679 5947 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1365 395 330 268 228 219 120 6 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6035 AflIII A'CryG_T Cuts at: 1203 5131 6108 1203 Size: 3928 977 1327 Fragments arranged by size: 3928 1327 977 AgeI A'CCGG_T Cuts at: 5063 5063 Size: 6232 AhdI GACnn_n'nnGTC Cuts at: 2734 4961 2734 Size: 2227 4005 AleI CACnn'nnGTG Cuts at: 1166 3002 1166 Size: 1836 4396 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6232 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2838 2870 2838 Size: 32 6200 AlwNI CAG_nnn'CTG Cuts at: 3975 5547 3975 Size: 1572 4660 ApaLI G'TGCA_C Cuts at: 4289 5445 4289 Size: 1156 5076 ApoI r'AATT_y Cuts at: 1493 2162 2513 3541 3767 3919 6028 1493 Size: 669 351 1028 226 152 2109 1697 Fragments arranged by size: 2109 1697 1028 669 351 226 152 AseI AT'TA_AT Cuts at: 160 1032 4786 160 Size: 872 3754 1606 Fragments arranged by size: 3754 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 AvaI C'yCGr_G Cuts at: 1870 1943 2979 3557 1870 Size: 73 1036 578 4545 Fragments arranged by size: 4545 1036 578 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2274 2307 2274 Size: 33 6199 BamHI G'GATC_C Cuts at: 2860 3562 3929 2860 Size: 702 367 5163 Fragments arranged by size: 5163 702 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3133 3553 5008 618 Size: 325 217 627 1346 420 1455 1842 Fragments arranged by size: 1842 1455 1346 627 420 325 217 BanII G_rGCy'C Cuts at: 1391 2376 3057 3150 3551 1391 Size: 985 681 93 401 4072 Fragments arranged by size: 4072 985 681 401 93 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2337 3486 928 Size: 971 438 1149 3674 Fragments arranged by size: 3674 1149 971 438 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4423 4457 1903 Size: 34 2486 34 3678 Fragments arranged by size: 3678 2486 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2845 4124 5334 2845 Size: 1279 1210 3743 Fragments arranged by size: 3743 1279 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6232 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4843 136 Size: 107 122 71 631 605 3171 1525 Fragments arranged by size: 3171 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6227 6227 Size: 6232 BlpI GC'TnA_GC Cuts at: 3149 3318 3649 3149 Size: 169 331 5732 Fragments arranged by size: 5732 331 169 Bme1580I G_kGCm'C Cuts at: 1790 2189 4293 5449 1790 Size: 399 2104 1156 2573 Fragments arranged by size: 2573 2104 1156 399 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6232 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3009 3963 4921 Size: 416 53 83 399 24 1592 954 958 Cuts at: 4921 442 Size: 1753 Fragments arranged by size: 1753 1592 958 954 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6232 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2994 2994 Size: 6232 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2045 2840 3102 4892 1239 Size: 806 795 262 1790 2579 Fragments arranged by size: 2579 1790 806 795 262 Bpu10I CC'TnA_GC Cuts at: 2969 2969 Size: 6232 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2051 3401 4357 5222 5520 2051 Size: 1350 956 865 298 2763 Fragments arranged by size: 2763 1350 956 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2898 4895 882 Size: 617 1399 1997 2219 Fragments arranged by size: 2219 1997 1399 617 BsaAI yAC'GTr Cuts at: 493 1204 4023 493 Size: 711 2819 2702 Fragments arranged by size: 2819 2702 711 BsaBI GATnn'nnATC Cuts at: 6226 6226 Size: 6232 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4039 4421 5056 275 Size: 53 83 186 936 2506 382 635 1451 Fragments arranged by size: 2506 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4665 5063 5337 5484 5895 4665 Size: 398 274 147 411 5002 Fragments arranged by size: 5002 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2040 2070 2363 2393 2838 Size: 30 165 30 637 30 293 30 445 Cuts at: 2838 2868 2942 2972 1178 Size: 30 74 30 4438 Fragments arranged by size: 4438 637 445 293 165 74 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2042 2114 2117 2701 3221 3386 1186 Size: 856 72 3 584 520 165 4032 Fragments arranged by size: 4032 856 584 520 165 72 3 BseYI C'CCAG_C Cuts at: 2137 2485 2540 3034 3305 5435 5931 2137 Size: 348 55 494 271 2130 496 2438 Fragments arranged by size: 2438 2130 496 494 348 271 55 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2289 3234 1163 Size: 573 553 945 4161 Fragments arranged by size: 4161 945 573 553 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2644 3682 4443 5471 1540 Size: 275 186 643 1038 761 1028 2301 Fragments arranged by size: 2301 1038 1028 761 643 275 186 BsiHKAI G_wGCw'C Cuts at: 2172 2376 4293 4378 5449 2172 Size: 204 1917 85 1071 2955 Fragments arranged by size: 2955 1917 1071 204 85 BsmI GAATG_Cn' Cuts at: 2649 3743 3836 2649 Size: 1094 93 5045 Fragments arranged by size: 5045 1094 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2172 2189 2376 3057 3150 3551 4293 Size: 399 382 17 187 681 93 401 742 Cuts at: 4293 4378 5449 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 681 401 399 382 187 93 85 17 BspEI T'CCGG_A Cuts at: 5895 5895 Size: 6232 BspHI T'CATG_A Cuts at: 4121 5851 4121 Size: 1730 4502 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3587 844 Size: 2743 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3166 4119 1394 Size: 429 1343 953 3507 Fragments arranged by size: 3507 1343 953 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2430 2535 4727 4901 65 Size: 1183 1182 105 2192 174 1396 Fragments arranged by size: 2192 1396 1183 1182 174 105 BsrFI r'CCGG_y Cuts at: 1700 1810 4876 5063 1700 Size: 110 3066 187 2869 Fragments arranged by size: 3066 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3327 96 Size: 3231 3001 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6232 BssSI C'ACGA_G Cuts at: 4292 5304 4292 Size: 1012 5220 BstAPI GCAn_nnn'nTGC Cuts at: 794 2015 794 Size: 1221 5011 BstBI TT'CG_AA Cuts at: 5036 5036 Size: 6232 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6232 BstXI CCAn_nnnn'nTGG Cuts at: 2825 2825 Size: 6232 BstYI r'GATC_y Cuts at: 1972 2595 2860 3562 3929 4314 4331 5783 5869 Size: 623 265 702 367 385 17 1452 86 Cuts at: 5869 5881 6227 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 702 623 385 367 346 265 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2787 2787 Size: 6232 BtgI C'CryG_G Cuts at: 513 1065 2004 2263 2497 3222 513 Size: 552 939 259 234 725 3523 Fragments arranged by size: 3523 939 725 552 259 234 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5493 Fragments arranged by size: 5493 683 56 BtsI GCAGTG_nn' Cuts at: 3024 3076 3748 4542 4562 3024 Size: 52 672 794 20 4694 Fragments arranged by size: 4694 794 672 52 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6232 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6197 DraI TTT'AAA Cuts at: 3536 3536 Size: 6232 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2627 5239 809 Size: 507 1311 2612 1802 Fragments arranged by size: 2612 1802 1311 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2500 3679 4568 Size: 54 1425 50 275 18 670 1179 889 Cuts at: 4568 6197 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1179 889 670 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3679 1537 Size: 275 1867 4090 Fragments arranged by size: 4090 1867 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2739 3045 3453 3469 4162 1353 Size: 1386 306 408 16 693 3423 Fragments arranged by size: 3423 1386 693 408 306 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4819 5193 5339 387 Size: 1064 138 3230 374 146 1280 Fragments arranged by size: 3230 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2374 2374 Size: 6232 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2027 2045 2147 2375 2594 2600 2840 Size: 393 395 18 102 228 219 6 240 Cuts at: 2840 2930 3102 4295 4892 5679 5947 1239 Size: 90 172 1193 597 787 268 1524 Fragments arranged by size: 1524 1193 787 597 395 393 268 240 228 219 172 102 90 18 6 EcoNI CCTnn'n_nnAGG Cuts at: 2630 3488 2630 Size: 858 5374 EcoO109I rG'GnC_Cy Cuts at: 2141 2776 2896 2141 Size: 635 120 5477 Fragments arranged by size: 5477 635 120 EcoRI G'AATT_C Cuts at: 2513 3541 3919 2513 Size: 1028 378 4826 Fragments arranged by size: 4826 1028 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2905 2935 2905 Size: 30 6202 FspI TGC'GCA Cuts at: 4738 4738 Size: 6232 HaeII r_GCGC'y Cuts at: 1678 2024 2088 2263 5379 1678 Size: 346 64 175 3116 2531 Fragments arranged by size: 3116 2531 346 175 64 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2363 2395 2720 2752 2942 Size: 32 135 32 791 32 325 32 190 Cuts at: 2942 2974 4873 4905 4947 4979 1373 Size: 32 1899 32 42 32 2626 Fragments arranged by size: 2626 1899 791 325 190 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3576 3822 669 Size: 988 281 1638 246 3079 Fragments arranged by size: 3079 1638 988 281 246 HindIII A'AGCT_T Cuts at: 748 2339 3281 748 Size: 1591 942 3699 Fragments arranged by size: 3699 1591 942 HpaI GTT'AAC Cuts at: 3822 3822 Size: 6232 KpnI G_GTAC'C Cuts at: 3557 3557 Size: 6232 MfeI C'AATT_G Cuts at: 3831 3831 Size: 6232 MluI A'CGCG_T Cuts at: 6108 6108 Size: 6232 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5346 5530 1305 Size: 4041 184 2007 Fragments arranged by size: 4041 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4410 Fragments arranged by size: 4410 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3002 3158 4190 4549 4708 518 Size: 648 1836 156 1032 359 159 2042 Fragments arranged by size: 2042 1836 1032 648 359 159 156 MspA1I CmG'CkG Cuts at: 1606 2137 2851 3648 4325 5473 5718 1606 Size: 531 714 797 677 1148 245 2120 Fragments arranged by size: 2120 1148 797 714 677 531 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4741 Fragments arranged by size: 4741 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6232 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6232 NotI GC'GGCC_GC Cuts at: 3679 3679 Size: 6232 NruI TCG'CGA Cuts at: 2223 2223 Size: 6232 NspI r_CATG'y Cuts at: 1132 1229 3112 3590 5135 1132 Size: 97 1883 478 1545 2229 Fragments arranged by size: 2229 1883 1545 478 97 PasI CC'CwG_GG Cuts at: 1348 3058 1348 Size: 1710 4522 PciI A'CATG_T Cuts at: 5131 5131 Size: 6232 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6232 PfoI T'CCnGG_A Cuts at: 2856 2856 Size: 6232 PmeI GTTT'AAAC Cuts at: 3536 3536 Size: 6232 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2838 2870 4301 4333 5814 5846 2838 Size: 32 1431 32 1481 32 3224 Fragments arranged by size: 3224 1481 1431 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2141 2141 Size: 6232 PshAI GACnn'nnGTC Cuts at: 1613 2199 1613 Size: 586 5646 PsiI TTA'TAA Cuts at: 3802 3802 Size: 6232 PspXI vC'TCGA_Gb Cuts at: 2979 2979 Size: 6232 PstI C_TGCA'G Cuts at: 830 3584 830 Size: 2754 3478 PvuI CG_AT'CG Cuts at: 2001 2644 2001 Size: 643 5589 PvuII CAG'CTG Cuts at: 2137 2851 2137 Size: 714 5518 SacI G_AGCT'C Cuts at: 2376 2376 Size: 6232 SalI G'TCGA_C Cuts at: 1936 3574 1936 Size: 1638 4594 SapI GCTCTTCn'nnn_ Cuts at: 3045 3045 Size: 6232 SbfI CC_TGCA'GG Cuts at: 3584 3584 Size: 6232 ScaI AGT'ACT Cuts at: 3365 4480 6192 3365 Size: 1115 1712 3405 Fragments arranged by size: 3405 1712 1115 SfcI C'TryA_G Cuts at: 826 1045 2197 3026 3580 4715 5396 5587 826 Size: 219 1152 829 554 1135 681 191 1471 Fragments arranged by size: 1471 1152 1135 829 681 554 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6232 SmaI CCC'GGG Cuts at: 1872 3559 1872 Size: 1687 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2066 2979 3416 4336 5237 5499 Size: 197 926 123 913 437 920 901 262 Cuts at: 5499 820 Size: 1553 Fragments arranged by size: 1553 926 920 913 901 437 262 197 123 SnaBI TAC'GTA Cuts at: 493 4023 493 Size: 3530 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6232 SphI G_CATG'C Cuts at: 1132 3590 1132 Size: 2458 3774 SspI AAT'ATT Cuts at: 5 52 4156 5 Size: 47 4104 2081 Fragments arranged by size: 4104 2081 47 StuI AGG'CCT Cuts at: 1517 2064 2888 1517 Size: 547 824 4861 Fragments arranged by size: 4861 824 547 StyI C'CwwG_G Cuts at: 513 1065 2004 2181 2889 3671 513 Size: 552 939 177 708 782 3074 Fragments arranged by size: 3074 939 782 708 552 177 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3050 4259 4276 4429 4614 5059 325 Size: 1255 1470 1209 17 153 185 445 1498 Fragments arranged by size: 1498 1470 1255 1209 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3327 3363 4478 Size: 274 80 33 51 1443 1350 36 1115 Cuts at: 4478 6190 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1350 1115 274 138 80 51 36 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2040 2072 6192 6224 2040 Size: 32 4120 32 2048 Fragments arranged by size: 4120 2048 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2813 1316 Size: 1497 4735 XbaI T'CTAG_A Cuts at: 2653 3568 2653 Size: 915 5317 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2825 2825 Size: 6232 XhoI C'TCGA_G Cuts at: 1943 2979 1943 Size: 1036 5196 XmaI C'CCGG_G Cuts at: 1870 3557 1870 Size: 1687 4545 XmnI GAAnn'nnTTC Cuts at: 4361 4361 Size: 6232 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4040 276 Size: 53 83 186 936 2506 2468 Fragments arranged by size: 2506 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI