Restriction map for
pFN21AB5209
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AvaI AviII AvrII
BbeI BbvCI BciVI BfrI BlnI
BlpI Bpu1102I BsaI BsaWI BseAI
BsiEI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrGI
BssHII Bst1107I BstBI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoRV EcoT22I FseI FspI
HaeII HincII HindII HindIII HpaI
KasI KpnI KspI MfeI MluI
MroI MunI NarI NcoI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcsI 964 GAATTC [GA]^AATT[CT]
AcyI 114 GGCGTC G[GA]^CG[CT]C
AlwNI 847 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 541 CCTCAGG CC^T[ACGT]AGG
ApoI 964 GAATTC [GA]^AATT[CT]
BamHI 1419 GGATCC G^GATCC
BbrPI 1204 CACGTG CAC^GTG
BcgI 1544 GCAATGCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1362 TGATCA T^GATCA
BcnI 51 CCCGG CC^[GC]GG
BglII 797 AGATCT A^GATCT
BsaBI 1395 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 114 GGCGTC G[GA]^CG[CT]C
BsiWI 1097 CGTACG C^GTACG
BsrFI 46 GCCGGC [GA]^CCGG[CT]
BssSI 922 CACGAG CACGAG(-5/-1)
BstAPI 138 GCACTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 981 GGTGACC G^GT[ACGT]ACC
BstPI 981 GGTGACC G^GT[ACGT]ACC
Bsu36I 541 CCTCAGG CC^T[ACGT]AGG
Cfr10I 46 GCCGGC [GA]^CCGG[CT]
Eco81I 541 CCTCAGG CC^T[ACGT]AGG
EcoO65I 981 GGTGACC G^GT[ACGT]ACC
EcoRI 964 GAATTC G^AATTC
FbaI 1362 TGATCA T^GATCA
Hin1I 114 GGCGTC G[GA]^CG[CT]C
MamI 1395 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 603 CATTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 46 GCCGGC GCC^GGC
NciI 51 CCCGG CC^[GC]GG
NgoMIV 46 GCCGGC G^CCGGC
PmaCI 1204 CACGTG CAC^GTG
PmlI 1204 CACGTG CAC^GTG
PpuMI 926 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 849 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SmlI 1315 CTTGAG C^T[CT][GA]AG
SphI 1157 GCATGC GCATG^C
StuI 659 AGGCCT AGG^CCT
TfiI 444 GAATC G^A[AT]TC
XcmI 842 CCATGCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AccII 36 CGCG CG^CG
1181 CGCG
ApaI 699 GGGCCC GGGCC^C
1500 GGGCCC
AspHI 393 GTGCTC G[AT]GC[AT]^C
1044 GTGCTC
AspI 111 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
202 GACGTTGTC
BalI 20 TGGCCA TGG^CCA
515 TGGCCA
BanI 95 GGTGCC G^G[CT][GA]CC
1514 GGTGCC
BbsI 108 GAAGAC GAAGAC(2/6)
273 GAAGAC
BfaI 43 CTAG C^TAG
433 CTAG
BglI 26 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
862 GCCAAAGTGGC
BpuAI 108 GAAGAC GAAGAC(2/6)
273 GAAGAC
BsaAI 1095 CACGTA [CT]AC^GT[GA]
1204 CACGTG
BsiHKAI 393 GTGCTC G[AT]GC[AT]^C
1044 GTGCTC
BsmAI 1528 GAGAC GTCTC(1/5)
417 GTCTC
BsmI 70 GAATGC GAATGC(1/-1)
602 GCATTC
BspLU11I 302 ACATGT A^CATGT
672 ACATGT
BsrDI 1558 CATTGC GCAATG(2/0)
1544 GCAATG
BstUI 36 CGCG CG^CG
1181 CGCG
CfoI 35 GCGC GCG^C
805 GCGC
EarI 10 CTCTTC CTCTTC(1/4)
1072 GAAGAG
HapII 47 CCGG C^CGG
52 CCGG
HgaI 1027 GACGC GACGC(5/10)
115 GCGTC
HhaI 35 GCGC GCG^C
805 GCGC
HinP1I 35 GCGC G^CGC
805 GCGC
HpaII 47 CCGG C^CGG
52 CCGG
Ksp632I 10 CTCTTC CTCTTC(1/4)
1072 GAAGAG
MaeI 43 CTAG C^TAG
433 CTAG
MluNI 20 TGGCCA TGG^CCA
515 TGGCCA
MscI 20 TGGCCA TGG^CCA
515 TGGCCA
MseI 362 TTAA T^TAA
914 TTAA
MspA1I 215 CAGCTG C[AC]G^C[GT]G
457 CAGCTG
MspI 47 CCGG C^CGG
52 CCGG
MvnI 36 CGCG CG^CG
1181 CGCG
NdeI 192 CATATG CA^TATG
717 CATATG
PflFI 111 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
202 GACGTTGTC
PflMI 657 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1080 CCAGGAGGTGG
PstI 145 CTGCAG CTGCA^G
1326 CTGCAG
PvuII 215 CAGCTG CAG^CTG
457 CAGCTG
SfaNI 768 GATGC GCATC(5/9)
837 GATGC
SfcI 145 CTGCAG C^T[GA][CT]AG
1326 CTGCAG
Tru9I 362 TTAA T^TAA
914 TTAA
Tth111I 111 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
202 GACGTTGTC
Van91I 657 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1080 CCAGGAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AvaII BanII BbvI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII EaeI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII