(Circular) (Six-base) MAPSORT of: pFN21AB5060.seq Check: 5432 from: 1 to: 6460 pFN21AB5060 6460 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3606 4270 278 Size: 53 83 186 936 2070 664 2468 Fragments arranged by size: 2468 2070 936 664 186 83 53 AccI GT'mk_AC Cuts at: 1937 3447 3803 1937 Size: 1510 356 4594 Fragments arranged by size: 4594 1510 356 Acc65I G'GTAC_C Cuts at: 3781 3781 Size: 6460 AclI AA'CG_TT Cuts at: 1563 4587 4960 1563 Size: 3024 373 3063 Fragments arranged by size: 3063 3024 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2351 2990 4523 5907 6175 1632 Size: 719 639 1533 1384 268 1917 Fragments arranged by size: 1917 1533 1384 719 639 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6263 AflIII A'CryG_T Cuts at: 1203 5359 6336 1203 Size: 4156 977 1327 Fragments arranged by size: 4156 1327 977 AgeI A'CCGG_T Cuts at: 5291 5291 Size: 6460 AhdI GACnn_n'nnGTC Cuts at: 2314 5189 2314 Size: 2875 3585 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6460 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6460 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2756 2788 2756 Size: 32 6428 AlwNI CAG_nnn'CTG Cuts at: 2224 2557 2851 3145 3175 3270 4203 5775 2224 Size: 333 294 294 30 95 933 1572 2909 Fragments arranged by size: 2909 1572 933 333 294 294 95 30 ApaI G_GGCC'C Cuts at: 2197 2197 Size: 6460 ApaLI G'TGCA_C Cuts at: 2281 3276 4517 5673 2281 Size: 995 1241 1156 3068 Fragments arranged by size: 3068 1241 1156 995 ApoI r'AATT_y Cuts at: 1493 3149 3769 3995 4147 6256 1493 Size: 1656 620 226 152 2109 1697 Fragments arranged by size: 2109 1697 1656 620 226 152 AseI AT'TA_AT Cuts at: 160 1032 5014 160 Size: 872 3982 1606 Fragments arranged by size: 3982 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6460 AvaI C'yCGr_G Cuts at: 1870 1943 3785 1870 Size: 73 1842 4545 Fragments arranged by size: 4545 1842 73 BamHI G'GATC_C Cuts at: 3790 4157 3790 Size: 367 6093 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2093 2947 3781 5236 618 Size: 325 217 627 306 854 834 1455 1842 Fragments arranged by size: 1842 1455 854 834 627 325 306 217 BanII G_rGCy'C Cuts at: 1391 2197 2421 3072 3507 3591 3779 1391 Size: 806 224 651 435 84 188 4072 Fragments arranged by size: 4072 806 651 435 224 188 84 BbeI G_GCGC'C Cuts at: 2097 2097 Size: 6460 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3137 928 Size: 971 1238 4251 Fragments arranged by size: 4251 1238 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4651 4685 1903 Size: 34 2714 34 3678 Fragments arranged by size: 3678 2714 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3202 4352 5562 3202 Size: 1150 1210 4100 Fragments arranged by size: 4100 1210 1150 BclI T'GATC_A Cuts at: 1548 1548 Size: 6460 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2139 2219 2303 Size: 107 122 71 631 605 467 80 84 Cuts at: 2303 2457 2708 5071 136 Size: 154 251 2363 1525 Fragments arranged by size: 2363 1525 631 605 467 251 154 122 107 84 80 71 BglII A'GATC_T Cuts at: 2118 2439 6455 2118 Size: 321 4016 2123 Fragments arranged by size: 4016 2123 321 BlpI GC'TnA_GC Cuts at: 3877 3877 Size: 6460 Bme1580I G_kGCm'C Cuts at: 1790 2144 2197 2285 2613 2952 3165 3280 3646 Size: 354 53 88 328 339 213 115 366 Cuts at: 3646 3699 4521 5677 1790 Size: 53 822 1156 2573 Fragments arranged by size: 2573 1156 822 366 354 339 328 213 115 88 53 53 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6460 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2889 3633 4191 Size: 416 53 83 399 24 1472 744 558 Cuts at: 4191 5149 442 Size: 958 1753 Fragments arranged by size: 1753 1472 958 744 558 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6460 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3022 3022 Size: 6460 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2395 2564 2849 3007 3124 3265 3292 5120 Size: 1156 169 285 158 117 141 27 1828 Cuts at: 5120 1239 Size: 2579 Fragments arranged by size: 2579 1828 1156 285 169 158 141 117 27 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4585 5450 5748 4585 Size: 865 298 5297 Fragments arranged by size: 5297 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5123 882 Size: 617 3624 2219 Fragments arranged by size: 3624 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4251 493 Size: 711 3047 2702 Fragments arranged by size: 3047 2702 711 BsaBI GATnn'nnATC Cuts at: 2657 6454 2657 Size: 3797 2663 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2094 2499 2820 3603 Size: 53 83 186 936 561 405 321 783 Cuts at: 3603 4267 4649 5284 275 Size: 664 382 635 1451 Fragments arranged by size: 1451 936 783 664 635 561 405 382 321 186 83 53 BsaWI w'CCGG_w Cuts at: 4893 5291 5565 5712 6123 4893 Size: 398 274 147 411 5230 Fragments arranged by size: 5230 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2627 2657 3470 3500 1178 Size: 30 165 30 1224 30 813 30 4138 Fragments arranged by size: 4138 1224 813 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2744 3029 3080 3484 3664 3736 1186 Size: 1558 285 51 404 180 72 3910 Fragments arranged by size: 3910 1558 404 285 180 72 51 BseYI C'CCAG_C Cuts at: 2951 2991 3404 5663 6159 2951 Size: 40 413 2259 496 3252 Fragments arranged by size: 3252 2259 496 413 40 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2356 2507 1163 Size: 573 620 151 5116 Fragments arranged by size: 5116 620 573 151 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3910 4671 5699 1540 Size: 275 186 1909 761 1028 2301 Fragments arranged by size: 2301 1909 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2285 2393 2421 3072 3280 3507 3671 4521 4606 Size: 108 28 651 208 227 164 850 85 Cuts at: 4606 5677 2285 Size: 1071 3068 Fragments arranged by size: 3068 1071 850 651 227 208 164 108 85 28 BsmI GAATG_Cn' Cuts at: 3971 4064 3971 Size: 93 6367 BsmBI CGTCTCn'nnnn_ Cuts at: 2349 2349 Size: 6460 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2144 2197 2285 2393 2421 2613 2952 Size: 399 354 53 88 108 28 192 339 Cuts at: 2952 3072 3165 3280 3507 3591 3646 3671 3699 Size: 120 93 115 227 84 55 25 28 Cuts at: 3699 3779 4521 4606 5677 1391 Size: 80 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 354 339 227 192 120 115 108 93 88 85 84 80 55 53 28 28 25 BspEI T'CCGG_A Cuts at: 6123 6123 Size: 6460 BspHI T'CATG_A Cuts at: 4349 6079 4349 Size: 1730 4730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2919 3815 844 Size: 2075 896 3489 Fragments arranged by size: 3489 2075 896 BsrBI CCG'CTC Cuts at: 1394 1823 4347 1394 Size: 429 2524 3507 Fragments arranged by size: 3507 2524 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2584 3549 4955 5129 65 Size: 1183 1336 965 1406 174 1396 Fragments arranged by size: 1406 1396 1336 1183 965 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5104 5291 1700 Size: 110 3294 187 2869 Fragments arranged by size: 3294 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6460 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6460 BssSI C'ACGA_G Cuts at: 2348 3110 4520 5532 2348 Size: 762 1410 1012 3276 Fragments arranged by size: 3276 1410 1012 762 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6460 BstBI TT'CG_AA Cuts at: 5264 5264 Size: 6460 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6460 BstXI CCAn_nnnn'nTGG Cuts at: 2266 2593 2959 3646 2266 Size: 327 366 687 5080 Fragments arranged by size: 5080 687 366 327 BstYI r'GATC_y Cuts at: 1972 2118 2439 3039 3790 4157 4542 4559 6011 Size: 146 321 600 751 367 385 17 1452 Cuts at: 6011 6097 6109 6455 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 751 600 385 367 346 321 146 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3063 3748 3063 Size: 685 5775 BtgI C'CryG_G Cuts at: 513 1065 2613 2976 513 Size: 552 1548 363 3997 Fragments arranged by size: 3997 1548 552 363 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5721 Fragments arranged by size: 5721 683 56 BtsI GCAGTG_nn' Cuts at: 2811 3976 4770 4790 2811 Size: 1165 794 20 4481 Fragments arranged by size: 4481 1165 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6460 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6425 DraI TTT'AAA Cuts at: 3764 3764 Size: 6460 DraIII CAC_nnn'GTG Cuts at: 2148 3160 2148 Size: 1012 5448 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5467 809 Size: 507 4151 1802 Fragments arranged by size: 4151 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2082 2304 2434 Size: 54 1425 50 275 18 252 222 130 Cuts at: 2434 2515 2808 3907 4796 6425 8 Size: 81 293 1099 889 1629 43 Fragments arranged by size: 1629 1425 1099 889 293 275 252 222 130 81 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3907 1537 Size: 275 2095 4090 Fragments arranged by size: 4090 2095 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2027 2327 3419 3596 3659 4390 1353 Size: 674 300 1092 177 63 731 3423 Fragments arranged by size: 3423 1092 731 674 300 177 63 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3713 5047 5421 5567 387 Size: 1064 138 2124 1334 374 146 1280 Fragments arranged by size: 2124 1334 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2419 3070 3505 2419 Size: 651 435 5374 Fragments arranged by size: 5374 651 435 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2351 2395 2564 2849 2990 3007 3124 Size: 393 719 44 169 285 141 17 117 Cuts at: 3124 3265 3292 4523 5120 5907 6175 1239 Size: 141 27 1231 597 787 268 1524 Fragments arranged by size: 1524 1231 787 719 597 393 285 268 169 141 141 117 44 27 17 EcoNI CCTnn'n_nnAGG Cuts at: 2561 2759 2969 3203 3416 2561 Size: 198 210 234 213 5605 Fragments arranged by size: 5605 234 213 210 198 EcoO109I rG'GnC_Cy Cuts at: 2164 2194 2255 2428 2554 2623 2764 3115 3142 Size: 30 61 173 126 69 141 351 27 Cuts at: 3142 3314 2164 Size: 172 5310 Fragments arranged by size: 5310 351 173 172 141 126 69 61 30 27 EcoRI G'AATT_C Cuts at: 3149 3769 4147 3149 Size: 620 378 5462 Fragments arranged by size: 5462 620 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3061 3061 Size: 6460 FspI TGC'GCA Cuts at: 2734 4966 2734 Size: 2232 4228 HaeII r_GCGC'y Cuts at: 1678 2097 5607 1678 Size: 419 3510 2531 Fragments arranged by size: 3510 2531 419 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2300 2332 5101 5133 5175 Size: 32 135 32 728 32 2769 32 42 Cuts at: 5175 5207 1373 Size: 32 2626 Fragments arranged by size: 2769 2626 728 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3804 4050 669 Size: 988 281 1866 246 3079 Fragments arranged by size: 3079 1866 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6460 HpaI GTT'AAC Cuts at: 4050 4050 Size: 6460 KasI G'GCGC_C Cuts at: 2093 2093 Size: 6460 KpnI G_GTAC'C Cuts at: 3785 3785 Size: 6460 MfeI C'AATT_G Cuts at: 4059 4059 Size: 6460 MluI A'CGCG_T Cuts at: 6336 6336 Size: 6460 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2941 5574 5758 1305 Size: 1636 2633 184 2007 Fragments arranged by size: 2633 2007 1636 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2436 2517 2810 10 Size: 54 1425 343 604 81 293 3660 Fragments arranged by size: 3660 1425 604 343 293 81 54 MslI CAynn'nnrTG Cuts at: 518 1166 2477 2591 4418 4777 4936 518 Size: 648 1311 114 1827 359 159 2042 Fragments arranged by size: 2042 1827 1311 648 359 159 114 MspA1I CmG'CkG Cuts at: 1606 2455 2719 3876 4553 5701 5946 1606 Size: 849 264 1157 677 1148 245 2120 Fragments arranged by size: 2120 1157 1148 849 677 264 245 NarI GG'CG_CC Cuts at: 2094 2094 Size: 6460 NcoI C'CATG_G Cuts at: 513 1065 2613 513 Size: 552 1548 4360 Fragments arranged by size: 4360 1548 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6460 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6460 NotI GC'GGCC_GC Cuts at: 3907 3907 Size: 6460 NspI r_CATG'y Cuts at: 1132 1229 2077 3818 5363 1132 Size: 97 848 1741 1545 2229 Fragments arranged by size: 2229 1741 1545 848 97 PasI CC'CwG_GG Cuts at: 1348 2197 2320 2503 1348 Size: 849 123 183 5305 Fragments arranged by size: 5305 849 183 123 PciI A'CATG_T Cuts at: 5359 5359 Size: 6460 PflMI CCAn_nnn'nTGG Cuts at: 1108 2224 2401 2692 3175 1108 Size: 1116 177 291 483 4393 Fragments arranged by size: 4393 1116 483 291 177 PfoI T'CCnGG_A Cuts at: 3269 3417 3269 Size: 148 6312 PmeI GTTT'AAAC Cuts at: 3764 3764 Size: 6460 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3068 3100 3104 3136 4529 4561 6042 6074 3068 Size: 32 4 32 1393 32 1481 32 3454 Fragments arranged by size: 3454 1481 1393 32 32 32 32 4 PpuMI rG'GwC_Cy Cuts at: 2164 2623 2764 2164 Size: 459 141 5860 Fragments arranged by size: 5860 459 141 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6460 PsiI TTA'TAA Cuts at: 4030 4030 Size: 6460 PspOMI G'GGCC_C Cuts at: 2193 2193 Size: 6460 PstI C_TGCA'G Cuts at: 830 2345 2724 3084 3141 3812 830 Size: 1515 379 360 57 671 3478 Fragments arranged by size: 3478 1515 671 379 360 57 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6460 PvuII CAG'CTG Cuts at: 2719 2719 Size: 6460 SacI G_AGCT'C Cuts at: 2421 3072 3507 2421 Size: 651 435 5374 Fragments arranged by size: 5374 651 435 SalI G'TCGA_C Cuts at: 1936 3802 1936 Size: 1866 4594 SapI GCTCTTCn'nnn_ Cuts at: 2027 2327 3596 3659 2027 Size: 300 1269 63 4828 Fragments arranged by size: 4828 1269 300 63 SbfI CC_TGCA'GG Cuts at: 3812 3812 Size: 6460 ScaI AGT'ACT Cuts at: 4708 6420 4708 Size: 1712 4748 SfcI C'TryA_G Cuts at: 826 1045 2125 2341 2720 2843 3080 3137 3361 Size: 219 1080 216 379 123 237 57 224 Cuts at: 3361 3808 4943 5624 5815 826 Size: 447 1135 681 191 1471 Fragments arranged by size: 1471 1135 1080 681 447 379 237 224 219 216 191 123 57 SfiI GGCCn_nnn'nGGCC Cuts at: 2303 2303 Size: 6460 SfoI GGC'GCC Cuts at: 2095 2095 Size: 6460 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6460 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6460 SmaI CCC'GGG Cuts at: 1872 3787 1872 Size: 1915 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4564 5465 5727 820 Size: 197 926 2621 901 262 1553 Fragments arranged by size: 2621 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4251 493 Size: 3758 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6460 SphI G_CATG'C Cuts at: 1132 3818 1132 Size: 2686 3774 SspI AAT'ATT Cuts at: 5 52 3329 4384 5 Size: 47 3277 1055 2081 Fragments arranged by size: 3277 2081 1055 47 StuI AGG'CCT Cuts at: 1517 2297 2870 2888 3192 1517 Size: 780 573 18 304 4785 Fragments arranged by size: 4785 780 573 304 18 StyI C'CwwG_G Cuts at: 513 1065 2053 2431 2613 3899 513 Size: 552 988 378 182 1286 3074 Fragments arranged by size: 3074 1286 988 552 378 182 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2017 2231 2408 4487 4504 4657 4842 Size: 1255 437 214 177 2079 17 153 185 Cuts at: 4842 5287 325 Size: 445 1498 Fragments arranged by size: 2079 1498 1255 445 437 214 185 177 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3099 4706 6418 Size: 274 80 33 51 1443 1122 1607 1712 Cuts at: 6418 96 Size: 138 Fragments arranged by size: 1712 1607 1443 1122 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2588 2620 6420 6452 2588 Size: 32 3800 32 2596 Fragments arranged by size: 3800 2596 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2273 1316 Size: 957 5503 XbaI T'CTAG_A Cuts at: 3796 3796 Size: 6460 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2221 2938 2221 Size: 717 5743 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6460 XmaI C'CCGG_G Cuts at: 1870 3785 1870 Size: 1915 4545 XmnI GAAnn'nnTTC Cuts at: 4589 4589 Size: 6460 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3604 4268 276 Size: 53 83 186 936 2070 664 2468 Fragments arranged by size: 2468 2070 936 664 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SrfI SwaI