(Circular) (Six-base) MAPSORT of: pFN21AE0971.seq Check: 8132 from: 1 to: 6325 pFN21AE0971 6325 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2157 2157 Size: 6325 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4135 278 Size: 53 83 186 936 2599 2468 Fragments arranged by size: 2599 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3668 1937 Size: 1731 4594 Acc65I G'GTAC_C Cuts at: 3646 3646 Size: 6325 AclI AA'CG_TT Cuts at: 1563 4452 4825 1563 Size: 2889 373 3063 Fragments arranged by size: 3063 2889 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3234 3539 4388 5772 6040 1632 Size: 1602 305 849 1384 268 1917 Fragments arranged by size: 1917 1602 1384 849 305 268 AflII C'TTAA_G Cuts at: 820 1017 2194 820 Size: 197 1177 4951 Fragments arranged by size: 4951 1177 197 AflIII A'CryG_T Cuts at: 1203 5224 6201 1203 Size: 4021 977 1327 Fragments arranged by size: 4021 1327 977 AgeI A'CCGG_T Cuts at: 5156 5156 Size: 6325 AhdI GACnn_n'nnGTC Cuts at: 5054 5054 Size: 6325 AleI CACnn'nnGTG Cuts at: 1166 2973 1166 Size: 1807 4518 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2048 2403 1184 Size: 864 355 5106 Fragments arranged by size: 5106 864 355 AlwNI CAG_nnn'CTG Cuts at: 4068 5640 4068 Size: 1572 4753 ApaLI G'TGCA_C Cuts at: 4382 5538 4382 Size: 1156 5169 ApoI r'AATT_y Cuts at: 1493 2208 2253 2270 2474 3503 3601 3634 3860 Size: 715 45 17 204 1029 98 33 226 Cuts at: 3860 4012 6121 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1029 715 226 204 152 98 45 33 17 AseI AT'TA_AT Cuts at: 160 1032 2754 4879 160 Size: 872 1722 2125 1606 Fragments arranged by size: 2125 1722 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6325 AvaI C'yCGr_G Cuts at: 1870 1943 3650 1870 Size: 73 1707 4545 Fragments arranged by size: 4545 1707 73 BamHI G'GATC_C Cuts at: 3044 3321 3655 4022 3044 Size: 277 334 367 5347 Fragments arranged by size: 5347 367 334 277 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2084 3646 5101 618 Size: 325 217 627 297 1562 1455 1842 Fragments arranged by size: 1842 1562 1455 627 325 297 217 BanII G_rGCy'C Cuts at: 1391 3644 1391 Size: 2253 4072 BbeI G_GCGC'C Cuts at: 2088 2088 Size: 6325 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2453 3345 3545 928 Size: 971 554 892 200 3708 Fragments arranged by size: 3708 971 892 554 200 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4516 4550 1903 Size: 34 2579 34 3678 Fragments arranged by size: 3678 2579 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4217 5427 4217 Size: 1210 5115 BclI T'GATC_A Cuts at: 1548 3226 3408 1548 Size: 1678 182 4465 Fragments arranged by size: 4465 1678 182 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4936 136 Size: 107 122 71 631 605 3264 1525 Fragments arranged by size: 3264 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6320 6320 Size: 6325 BlpI GC'TnA_GC Cuts at: 3742 3742 Size: 6325 Bme1580I G_kGCm'C Cuts at: 1790 2827 4386 5542 1790 Size: 1037 1559 1156 2573 Fragments arranged by size: 2573 1559 1156 1037 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6325 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4056 5014 442 Size: 416 53 83 399 24 2639 958 1753 Fragments arranged by size: 2639 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6325 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4985 1239 Size: 3746 2579 Bpu10I CC'TnA_GC Cuts at: 2075 2075 Size: 6325 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3090 3120 4450 5315 5613 3090 Size: 30 1330 865 298 3802 Fragments arranged by size: 3802 1330 865 298 30 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4988 882 Size: 617 3489 2219 Fragments arranged by size: 3489 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2795 4116 493 Size: 711 1591 1321 2702 Fragments arranged by size: 2702 1591 1321 711 BsaBI GATnn'nnATC Cuts at: 3592 6319 3592 Size: 2727 3598 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2085 4132 4514 5149 Size: 53 83 186 936 552 2047 382 635 Cuts at: 5149 275 Size: 1451 Fragments arranged by size: 2047 1451 936 635 552 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4758 5156 5430 5577 5988 4758 Size: 398 274 147 411 5095 Fragments arranged by size: 5095 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6100 Fragments arranged by size: 6100 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2412 3093 1186 Size: 1226 681 4418 Fragments arranged by size: 4418 1226 681 BseYI C'CCAG_C Cuts at: 3312 5528 6024 3312 Size: 2216 496 3613 Fragments arranged by size: 3613 2216 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2135 2393 2538 1163 Size: 573 399 258 145 4950 Fragments arranged by size: 4950 573 399 258 145 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3775 4536 5564 1540 Size: 275 186 1774 761 1028 2301 Fragments arranged by size: 2301 1774 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2794 4386 4471 5542 2794 Size: 1592 85 1071 3577 Fragments arranged by size: 3577 1592 1071 85 BsmI GAATG_Cn' Cuts at: 3218 3836 3929 3218 Size: 618 93 5614 Fragments arranged by size: 5614 618 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2794 2827 3644 4386 4471 5542 1391 Size: 399 1004 33 817 742 85 1071 2174 Fragments arranged by size: 2174 1071 1004 817 742 399 85 33 BspEI T'CCGG_A Cuts at: 5988 5988 Size: 6325 BspHI T'CATG_A Cuts at: 2437 3411 4214 5944 2437 Size: 974 803 1730 2818 Fragments arranged by size: 2818 1730 974 803 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2073 2157 2415 3680 844 Size: 1229 84 258 1265 3489 Fragments arranged by size: 3489 1265 1229 258 84 BsrBI CCG'CTC Cuts at: 1394 1823 4212 1394 Size: 429 2389 3507 Fragments arranged by size: 3507 2389 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2266 4820 4994 65 Size: 1183 1018 2554 174 1396 Fragments arranged by size: 2554 1396 1183 1018 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4969 5156 1700 Size: 110 3159 187 2869 Fragments arranged by size: 3159 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6325 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6325 BssSI C'ACGA_G Cuts at: 4385 5397 4385 Size: 1012 5313 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6325 BstBI TT'CG_AA Cuts at: 5129 5129 Size: 6325 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6325 BstYI r'GATC_y Cuts at: 1972 3044 3203 3321 3655 4022 4407 4424 5876 Size: 1072 159 118 334 367 385 17 1452 Cuts at: 5876 5962 5974 6320 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1072 385 367 346 334 159 118 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2080 513 Size: 552 1015 4758 Fragments arranged by size: 4758 1015 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5586 Fragments arranged by size: 5586 683 56 BtsI GCAGTG_nn' Cuts at: 3323 3841 4635 4655 3323 Size: 518 794 20 4993 Fragments arranged by size: 4993 794 518 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6325 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6290 DraI TTT'AAA Cuts at: 3600 3629 3600 Size: 29 6296 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2126 5332 809 Size: 507 810 3206 1802 Fragments arranged by size: 3206 1802 810 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2136 2357 3772 Size: 54 1425 50 275 18 306 221 1415 Cuts at: 3772 4661 6290 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1415 889 306 275 221 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3772 1537 Size: 275 1960 4090 Fragments arranged by size: 4090 1960 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2892 3253 4255 1353 Size: 1539 361 1002 3423 Fragments arranged by size: 3423 1539 1002 361 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2095 4912 5286 5432 387 Size: 1064 138 506 2817 374 146 1280 Fragments arranged by size: 2817 1280 1064 506 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3234 3539 4388 4985 5772 6040 1239 Size: 393 1602 305 849 597 787 268 1524 Fragments arranged by size: 1602 1524 849 787 597 393 305 268 EcoO109I rG'GnC_Cy Cuts at: 3090 3453 3090 Size: 363 5962 EcoRI G'AATT_C Cuts at: 3634 4012 3634 Size: 378 5947 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2209 2865 3386 2209 Size: 656 521 5148 Fragments arranged by size: 5148 656 521 FspI TGC'GCA Cuts at: 4831 4831 Size: 6325 HaeII r_GCGC'y Cuts at: 1678 2031 2088 5472 1678 Size: 353 57 3384 2531 Fragments arranged by size: 3384 2531 353 57 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2358 2390 3107 3139 4966 Size: 32 135 32 786 32 717 32 1827 Cuts at: 4966 4998 5040 5072 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1827 786 717 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3669 3915 669 Size: 988 281 1731 246 3079 Fragments arranged by size: 3079 1731 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6325 HpaI GTT'AAC Cuts at: 3915 3915 Size: 6325 KasI G'GCGC_C Cuts at: 2084 2084 Size: 6325 KpnI G_GTAC'C Cuts at: 3650 3650 Size: 6325 MfeI C'AATT_G Cuts at: 3924 3924 Size: 6325 MluI A'CGCG_T Cuts at: 6201 6201 Size: 6325 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5439 5623 1305 Size: 4134 184 2007 Fragments arranged by size: 4134 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2359 10 Size: 54 1425 343 527 3976 Fragments arranged by size: 3976 1425 527 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2973 4283 4642 4801 518 Size: 648 1807 1310 359 159 2042 Fragments arranged by size: 2042 1807 1310 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2082 2167 3741 4418 5566 5811 1606 Size: 476 85 1574 677 1148 245 2120 Fragments arranged by size: 2120 1574 1148 677 476 245 85 NarI GG'CG_CC Cuts at: 2085 2085 Size: 6325 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5773 NdeI CA'TA_TG Cuts at: 387 2773 387 Size: 2386 3939 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6325 NotI GC'GGCC_GC Cuts at: 3772 3772 Size: 6325 NspI r_CATG'y Cuts at: 1132 1229 2299 3683 5228 1132 Size: 97 1070 1384 1545 2229 Fragments arranged by size: 2229 1545 1384 1070 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6325 PciI A'CATG_T Cuts at: 5224 5224 Size: 6325 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6325 PfoI T'CCnGG_A Cuts at: 3169 3169 Size: 6325 PmeI GTTT'AAAC Cuts at: 3629 3629 Size: 6325 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4394 4426 5907 5939 4394 Size: 32 1481 32 4780 Fragments arranged by size: 4780 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3090 3453 3090 Size: 363 5962 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6325 PsiI TTA'TAA Cuts at: 3895 3895 Size: 6325 PstI C_TGCA'G Cuts at: 830 2027 3280 3300 3677 830 Size: 1197 1253 20 377 3478 Fragments arranged by size: 3478 1253 1197 377 20 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6325 SacII CC_GC'GG Cuts at: 2083 2083 Size: 6325 SalI G'TCGA_C Cuts at: 1936 3667 1936 Size: 1731 4594 SapI GCTCTTCn'nnn_ Cuts at: 3253 3253 Size: 6325 SbfI CC_TGCA'GG Cuts at: 3677 3677 Size: 6325 ScaI AGT'ACT Cuts at: 4573 6285 4573 Size: 1712 4613 SfcI C'TryA_G Cuts at: 826 1045 2023 3276 3296 3673 4808 5489 5680 Size: 219 978 1253 20 377 1135 681 191 Cuts at: 5680 826 Size: 1471 Fragments arranged by size: 1471 1253 1135 978 681 377 219 191 20 SfoI GGC'GCC Cuts at: 2086 2086 Size: 6325 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6325 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6325 SmaI CCC'GGG Cuts at: 1872 3652 1872 Size: 1780 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2194 3105 3135 4429 5330 5592 Size: 197 926 251 911 30 1294 901 262 Cuts at: 5592 820 Size: 1553 Fragments arranged by size: 1553 1294 926 911 901 262 251 197 30 SnaBI TAC'GTA Cuts at: 493 4116 493 Size: 3623 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6325 SphI G_CATG'C Cuts at: 1132 3683 1132 Size: 2551 3774 SspI AAT'ATT Cuts at: 5 52 4249 5 Size: 47 4197 2081 Fragments arranged by size: 4197 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6325 StyI C'CwwG_G Cuts at: 513 1065 2392 3493 3764 513 Size: 552 1327 1101 271 3074 Fragments arranged by size: 3074 1327 1101 552 271 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4352 4369 4522 4707 5152 325 Size: 1255 2772 17 153 185 445 1498 Fragments arranged by size: 2772 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3520 4571 6283 Size: 274 80 33 51 1443 1543 1051 1712 Cuts at: 6283 96 Size: 138 Fragments arranged by size: 1712 1543 1443 1051 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6285 6317 6285 Size: 32 6293 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6325 XbaI T'CTAG_A Cuts at: 3125 3584 3661 3125 Size: 459 77 5789 Fragments arranged by size: 5789 459 77 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3065 3065 Size: 6325 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6325 XmaI C'CCGG_G Cuts at: 1870 3650 1870 Size: 1780 4545 XmnI GAAnn'nnTTC Cuts at: 4454 4454 Size: 6325 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4133 276 Size: 53 83 186 936 2599 2468 Fragments arranged by size: 2599 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SexAI SfiI SrfI SwaI