(Circular) (Six-base) MAPSORT of: pFN21AB4461.seq Check: 7450 from: 1 to: 6427 pFN21AB4461 6427 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2032 2032 Size: 6427 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4237 278 Size: 53 83 186 936 2701 2468 Fragments arranged by size: 2701 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3770 1937 Size: 1833 4594 Acc65I G'GTAC_C Cuts at: 3748 3748 Size: 6427 AclI AA'CG_TT Cuts at: 1563 4554 4927 1563 Size: 2991 373 3063 Fragments arranged by size: 3063 2991 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3426 4490 5874 6142 1632 Size: 1794 1064 1384 268 1917 Fragments arranged by size: 1917 1794 1384 1064 268 AfeI AGC'GCT Cuts at: 2632 2632 Size: 6427 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6230 AflIII A'CryG_T Cuts at: 1203 2376 5326 6303 1203 Size: 1173 2950 977 1327 Fragments arranged by size: 2950 1327 1173 977 AgeI A'CCGG_T Cuts at: 5258 5258 Size: 6427 AhdI GACnn_n'nnGTC Cuts at: 5156 5156 Size: 6427 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6427 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6427 AlwNI CAG_nnn'CTG Cuts at: 4170 5742 4170 Size: 1572 4855 ApaLI G'TGCA_C Cuts at: 4484 5640 4484 Size: 1156 5271 ApoI r'AATT_y Cuts at: 1493 3736 3962 4114 6223 1493 Size: 2243 226 152 2109 1697 Fragments arranged by size: 2243 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4981 160 Size: 872 3949 1606 Fragments arranged by size: 3949 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6427 AvaI C'yCGr_G Cuts at: 1870 1943 3752 1870 Size: 73 1809 4545 Fragments arranged by size: 4545 1809 73 BamHI G'GATC_C Cuts at: 3757 4124 3757 Size: 367 6060 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2021 2097 2668 3083 3748 Size: 325 217 627 234 76 571 415 665 Cuts at: 3748 5203 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 665 627 571 415 325 234 217 76 BanII G_rGCy'C Cuts at: 1391 3746 1391 Size: 2355 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5456 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4618 4652 1903 Size: 34 2681 34 3678 Fragments arranged by size: 3678 2681 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3338 3502 4319 5529 3338 Size: 164 817 1210 4236 Fragments arranged by size: 4236 1210 817 164 BclI T'GATC_A Cuts at: 1548 2847 2853 2964 1548 Size: 1299 6 111 5011 Fragments arranged by size: 5011 1299 111 6 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2352 3305 5038 Size: 107 122 71 631 605 680 953 1733 Cuts at: 5038 136 Size: 1525 Fragments arranged by size: 1733 1525 953 680 631 605 122 107 71 BglII A'GATC_T Cuts at: 6422 6422 Size: 6427 BlpI GC'TnA_GC Cuts at: 3844 3844 Size: 6427 Bme1580I G_kGCm'C Cuts at: 1790 2357 3086 4488 5644 1790 Size: 567 729 1402 1156 2573 Fragments arranged by size: 2573 1402 1156 729 567 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6427 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2414 2951 3327 Size: 416 53 83 399 24 997 537 376 Cuts at: 3327 4158 5116 442 Size: 831 958 1753 Fragments arranged by size: 1753 997 958 831 537 416 399 376 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6427 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2284 2726 5087 1239 Size: 1045 442 2361 2579 Fragments arranged by size: 2579 2361 1045 442 Bpu10I CC'TnA_GC Cuts at: 2334 2334 Size: 6427 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2435 4552 5417 5715 2435 Size: 2117 865 298 3147 Fragments arranged by size: 3147 2117 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5090 882 Size: 617 3591 2219 Fragments arranged by size: 3591 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4218 493 Size: 711 3014 2702 Fragments arranged by size: 3014 2702 711 BsaBI GATnn'nnATC Cuts at: 2690 2852 6421 2690 Size: 162 3569 2696 Fragments arranged by size: 3569 2696 162 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4234 4616 5251 275 Size: 53 83 186 936 2701 382 635 1451 Fragments arranged by size: 2701 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4860 5258 5532 5679 6090 4860 Size: 398 274 147 411 5197 Fragments arranged by size: 5197 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3023 3053 1178 Size: 30 165 30 1620 30 4552 Fragments arranged by size: 4552 1620 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2627 2690 3347 1186 Size: 1441 63 657 4266 Fragments arranged by size: 4266 1441 657 63 BseYI C'CCAG_C Cuts at: 2125 2461 3473 3522 5630 6126 2125 Size: 336 1012 49 2108 496 2426 Fragments arranged by size: 2426 2108 1012 496 336 49 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3609 1163 Size: 573 1873 3981 Fragments arranged by size: 3981 1873 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3877 4638 5666 1540 Size: 275 186 1876 761 1028 2301 Fragments arranged by size: 2301 1876 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4488 4573 5644 4488 Size: 85 1071 5271 Fragments arranged by size: 5271 1071 85 BsmI GAATG_Cn' Cuts at: 2061 3656 3938 4031 2061 Size: 1595 282 93 4457 Fragments arranged by size: 4457 1595 282 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2357 3086 3746 4488 4573 5644 1391 Size: 399 567 729 660 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 729 660 567 399 85 BspEI T'CCGG_A Cuts at: 6090 6090 Size: 6427 BspHI T'CATG_A Cuts at: 2259 2850 4316 6046 2259 Size: 591 1466 1730 2640 Fragments arranged by size: 2640 1730 1466 591 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2032 3782 844 Size: 1188 1750 3489 Fragments arranged by size: 3489 1750 1188 BsrBI CCG'CTC Cuts at: 1394 1823 4314 1394 Size: 429 2491 3507 Fragments arranged by size: 3507 2491 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2391 2751 2904 4922 5096 65 Size: 1183 1143 360 153 2018 174 1396 Fragments arranged by size: 2018 1396 1183 1143 360 174 153 BsrFI r'CCGG_y Cuts at: 1700 1810 2094 5071 5258 1700 Size: 110 284 2977 187 2869 Fragments arranged by size: 2977 2869 284 187 110 BsrGI T'GTAC_A Cuts at: 96 2949 96 Size: 2853 3574 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6427 BssSI C'ACGA_G Cuts at: 2356 4487 5499 2356 Size: 2131 1012 3284 Fragments arranged by size: 3284 2131 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3532 794 Size: 2738 3689 BstBI TT'CG_AA Cuts at: 2521 5231 2521 Size: 2710 3717 BstEII G'GTnAC_C Cuts at: 1184 3346 1184 Size: 2162 4265 BstXI CCAn_nnnn'nTGG Cuts at: 2044 2800 3196 3251 2044 Size: 756 396 55 5220 Fragments arranged by size: 5220 756 396 55 BstYI r'GATC_y Cuts at: 1972 3757 4124 4509 4526 5978 6064 6076 6422 Size: 1785 367 385 17 1452 86 12 346 Cuts at: 6422 1972 Size: 1977 Fragments arranged by size: 1977 1785 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2364 2983 3372 513 Size: 552 939 360 619 389 3568 Fragments arranged by size: 3568 939 619 552 389 360 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2749 487 Size: 683 56 1523 4165 Fragments arranged by size: 4165 1523 683 56 BtsI GCAGTG_nn' Cuts at: 2184 3072 3700 3943 4737 4757 2184 Size: 888 628 243 794 20 3854 Fragments arranged by size: 3854 888 794 628 243 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6427 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6392 DraI TTT'AAA Cuts at: 3731 3731 Size: 6427 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5434 809 Size: 507 4118 1802 Fragments arranged by size: 4118 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2344 3321 3874 Size: 54 1425 50 275 18 514 977 553 Cuts at: 3874 4763 6392 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 977 889 553 514 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3874 1537 Size: 275 2062 4090 Fragments arranged by size: 4090 2062 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2534 4357 1353 Size: 1181 1823 3423 Fragments arranged by size: 3423 1823 1181 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5014 5388 5534 387 Size: 1064 138 3425 374 146 1280 Fragments arranged by size: 3425 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2284 2726 3426 4490 5087 5874 6142 Size: 393 652 442 700 1064 597 787 268 Cuts at: 6142 1239 Size: 1524 Fragments arranged by size: 1524 1064 787 700 652 597 442 393 268 EcoO109I rG'GnC_Cy Cuts at: 2465 2701 2465 Size: 236 6191 EcoRI G'AATT_C Cuts at: 3736 4114 3736 Size: 378 6049 FspI TGC'GCA Cuts at: 4933 4933 Size: 6427 HaeII r_GCGC'y Cuts at: 1678 2634 5574 1678 Size: 956 2940 2531 Fragments arranged by size: 2940 2531 956 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2474 2506 2770 2802 3469 Size: 32 135 32 902 32 264 32 667 Cuts at: 3469 3501 3544 3576 5068 5100 5142 5174 1373 Size: 32 43 32 1492 32 42 32 2626 Fragments arranged by size: 2626 1492 902 667 264 135 43 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3771 4017 669 Size: 988 281 1833 246 3079 Fragments arranged by size: 3079 1833 988 281 246 HindIII A'AGCT_T Cuts at: 748 3063 748 Size: 2315 4112 HpaI GTT'AAC Cuts at: 4017 4017 Size: 6427 KpnI G_GTAC'C Cuts at: 3752 3752 Size: 6427 MfeI C'AATT_G Cuts at: 4026 4026 Size: 6427 MluI A'CGCG_T Cuts at: 6303 6303 Size: 6427 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3160 5541 5725 1305 Size: 1855 2381 184 2007 Fragments arranged by size: 2381 2007 1855 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4605 Fragments arranged by size: 4605 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2220 2264 2768 2855 3194 4385 4744 Size: 648 1054 44 504 87 339 1191 359 Cuts at: 4744 4903 518 Size: 159 2042 Fragments arranged by size: 2042 1191 1054 648 504 359 339 159 87 44 MspA1I CmG'CkG Cuts at: 1606 2461 3843 4520 5668 5913 1606 Size: 855 1382 677 1148 245 2120 Fragments arranged by size: 2120 1382 1148 855 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2364 513 Size: 552 939 360 4576 Fragments arranged by size: 4576 939 552 360 NdeI CA'TA_TG Cuts at: 387 387 Size: 6427 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6427 NotI GC'GGCC_GC Cuts at: 3874 3874 Size: 6427 NspI r_CATG'y Cuts at: 1132 1229 2380 3785 5330 1132 Size: 97 1151 1405 1545 2229 Fragments arranged by size: 2229 1545 1405 1151 97 PasI CC'CwG_GG Cuts at: 1348 2246 2872 3079 1348 Size: 898 626 207 4696 Fragments arranged by size: 4696 898 626 207 PciI A'CATG_T Cuts at: 2376 5326 2376 Size: 2950 3477 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6427 PfoI T'CCnGG_A Cuts at: 2508 3261 2508 Size: 753 5674 PmeI GTTT'AAAC Cuts at: 3731 3731 Size: 6427 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4496 4528 6009 6041 4496 Size: 32 1481 32 4882 Fragments arranged by size: 4882 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6427 PsiI TTA'TAA Cuts at: 3997 3997 Size: 6427 PstI C_TGCA'G Cuts at: 830 2401 3695 3779 830 Size: 1571 1294 84 3478 Fragments arranged by size: 3478 1571 1294 84 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6427 PvuII CAG'CTG Cuts at: 2461 2461 Size: 6427 SalI G'TCGA_C Cuts at: 1936 3769 1936 Size: 1833 4594 SbfI CC_TGCA'GG Cuts at: 3779 3779 Size: 6427 ScaI AGT'ACT Cuts at: 2403 4675 6387 2403 Size: 2272 1712 2443 Fragments arranged by size: 2443 2272 1712 SfcI C'TryA_G Cuts at: 826 1045 2397 3691 3775 4910 5591 5782 826 Size: 219 1352 1294 84 1135 681 191 1471 Fragments arranged by size: 1471 1352 1294 1135 681 219 191 84 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6427 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6427 SmaI CCC'GGG Cuts at: 1872 3754 1872 Size: 1882 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2450 4531 5432 5694 820 Size: 197 926 507 2081 901 262 1553 Fragments arranged by size: 2081 1553 926 901 507 262 197 SnaBI TAC'GTA Cuts at: 493 4218 493 Size: 3725 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6427 SphI G_CATG'C Cuts at: 1132 3785 1132 Size: 2653 3774 SspI AAT'ATT Cuts at: 5 52 4351 5 Size: 47 4299 2081 Fragments arranged by size: 4299 2081 47 StuI AGG'CCT Cuts at: 1517 2325 2973 1517 Size: 808 648 4971 Fragments arranged by size: 4971 808 648 StyI C'CwwG_G Cuts at: 513 1065 2004 2364 3710 3866 513 Size: 552 939 360 1346 156 3074 Fragments arranged by size: 3074 1346 939 552 360 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3446 4454 4471 4624 4809 5254 325 Size: 1255 1866 1008 17 153 185 445 1498 Fragments arranged by size: 1866 1498 1255 1008 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2401 2949 4673 Size: 274 80 33 51 1443 424 548 1724 Cuts at: 4673 6385 96 Size: 1712 138 Fragments arranged by size: 1724 1712 1443 548 424 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3264 3296 6387 6419 3264 Size: 32 3091 32 3272 Fragments arranged by size: 3272 3091 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6427 XbaI T'CTAG_A Cuts at: 3763 3763 Size: 6427 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2800 3340 3502 2800 Size: 540 162 5725 Fragments arranged by size: 5725 540 162 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6427 XmaI C'CCGG_G Cuts at: 1870 3752 1870 Size: 1882 4545 XmnI GAAnn'nnTTC Cuts at: 4556 4556 Size: 6427 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4235 276 Size: 53 83 186 936 2701 2468 Fragments arranged by size: 2701 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI