Restriction map for
pFN21AB4185
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BmyI BpuAI BsaAI BsaBI
BsaI BsiHKAI BsiWI BsmAI BsmBI
Bsp1286I BspDI BspLU11I BspMI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoT14I FbaI FseI
FspI HindIII HpaI KasI KpnI
MamI MfeI MflI MluI MseI
MslI MunI NarI NciI NcoI
NdeI NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PstI PvuI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI StyI SwaI Tru9I
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 285 GACGTC GACGT^C
AccI 88 GTCTAC GT^[AC][GT]AC
AccIII 923 TCCGGA T^CCGGA
AcsI 850 GAATTT [GA]^AATT[CT]
AlwI 16 GGATC GGATC(4/5)
AlwNI 766 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 850 GAATTT [GA]^AATT[CT]
AvaI 69 CTCGGG C^[CT]CG[GA]G
BalI 539 TGGCCA TGG^CCA
BfaI 682 CTAG C^TAG
BseAI 923 TCCGGA T^CCGGA
BseRI 334 GAGGAG GAGGAG(10/8)
BsiEI 60 CGACCG CG[GA][CT]^CG
BsmFI 514 GGGAC GGGAC(10/14)
BsmI 927 GAATGC GAATGC(1/-1)
BsoBI 69 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1136 TGTACA T^GTACA
BspEI 923 TCCGGA T^CCGGA
BspHI 581 TCATGA T^CATGA
BsrFI 381 GCCGGC [GA]^CCGG[CT]
BsrGI 1136 TGTACA T^GTACA
Cfr10I 381 GCCGGC [GA]^CCGG[CT]
DraIII 896 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 577 CTCTTC CTCTTC(1/4)
EcoT22I 867 ATGCAT ATGCA^T
FauI 273 GCGGG CCCGC(4/6)
HaeII 369 GGCGCT [GA]GCGC^[CT]
HincII 241 GTCAAC GT[CT]^[GA]AC
HindII 241 GTCAAC GT[CT]^[GA]AC
Ksp632I 577 CTCTTC CTCTTC(1/4)
MaeI 682 CTAG C^TAG
MluNI 539 TGGCCA TGG^CCA
MroI 923 TCCGGA T^CCGGA
MscI 539 TGGCCA TGG^CCA
NaeI 381 GCCGGC GCC^GGC
NgoMIV 381 GCCGGC G^CCGGC
NsiI 867 ATGCAT ATGCA^T
PleI 547 GAGTC GAGTC(4/5)
PshAI 144 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 581 TCATGA T^CATGA
SfcI 1070 CTATAG C^T[GA][CT]AG
SspBI 1136 TGTACA T^GTACA
TaqI 819 TCGA T^CGA
TthHB8I 819 TCGA T^CGA
XcmI 501 CCATGAAGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 256 GACGCC G[GA]^CG[CT]C
285 GACGTC
AfaI 495 GTAC GT^AC
1137 GTAC
AvaII 143 GGACC G^G[AT]CC
515 GGACC
BlpI 478 GCTCAGC GC^T[ACGT]AGC
961 GCTCAGC
Bpu1102I 478 GCTCAGC GC^T[ACGT]AGC
961 GCTCAGC
BsaHI 256 GACGCC G[GA]^CG[CT]C
285 GACGTC
BsgI 207 GTGCAG GTGCAG(16/14)
262 GTGCAG
BsrBI 271 GAGCGG CCGCTC(-3/-3)
454 GAGCGG
BsrDI 939 CATTGC GCAATG(2/0)
1103 GCAATG
BssSI 268 CACGAG CACGAG(-5/-1)
466 CTCGTG
CelII 478 GCTCAGC GC^T[ACGT]AGC
961 GCTCAGC
DpnII 17 GATC ^GATC
780 GATC
EaeI 431 TGGCCG [CT]^GGCC[GA]
539 TGGCCA
HgaI 192 GACGC GACGC(5/10)
256 GACGC
Hin1I 256 GACGCC G[GA]^CG[CT]C
285 GACGTC
HphI 748 GGTGA GGTGA(8/7)
392 TCACC
KspI 395 CCGCGG CCGC^GG
434 CCGCGG
MaeII 286 ACGT A^CGT
1050 ACGT
MboI 17 GATC ^GATC
780 GATC
NdeII 17 GATC ^GATC
780 GATC
PpuMI 142 AGGACCT [GA]G^G[AT]CC[CT]
514 GGGACCC
RsaI 495 GTAC GT^AC
1137 GTAC
SacII 395 CCGCGG CCGC^GG
434 CCGCGG
Sau3AI 17 GATC ^GATC
780 GATC
TfiI 559 GAATC G^A[AT]TC
760 GATTC
Tsp509I 851 AATT ^AATT
1113 AATT
TspRI 205 CAGTG CA[GC]TG[ACGT][ACGT]^
1146 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AccII AciI AluI BbvI BpmI
BsaJI BsaWI BsiYI BslI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII DsaI
EcoO109I EcoRII EcoRV Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MaeIII MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NlaIII NlaIV PvuII Sau96I ScrFI
SfaNI TseI Tsp45I