(Circular) (Six-base) MAPSORT of: pFN21AB4185.seq Check: 8899 from: 1 to: 5860 pFN21AB4185 5860 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2294 3670 278 Size: 53 83 186 936 758 1376 2468 Fragments arranged by size: 2468 1376 936 758 186 83 53 AccI GT'mk_AC Cuts at: 1937 2094 3203 1937 Size: 157 1109 4594 Fragments arranged by size: 4594 1109 157 Acc65I G'GTAC_C Cuts at: 3181 3181 Size: 5860 AclI AA'CG_TT Cuts at: 1563 3987 4360 1563 Size: 2424 373 3063 Fragments arranged by size: 3063 2424 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2249 3923 5307 5575 1632 Size: 617 1674 1384 268 1917 Fragments arranged by size: 1917 1674 1384 617 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5663 AflIII A'CryG_T Cuts at: 1203 4759 5736 1203 Size: 3556 977 1327 Fragments arranged by size: 3556 1327 977 AgeI A'CCGG_T Cuts at: 4691 4691 Size: 5860 AhdI GACnn_n'nnGTC Cuts at: 4589 4589 Size: 5860 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5860 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5860 AlwNI CAG_nnn'CTG Cuts at: 2776 3603 5175 2776 Size: 827 1572 3461 Fragments arranged by size: 3461 1572 827 ApaLI G'TGCA_C Cuts at: 3917 5073 3917 Size: 1156 4704 ApoI r'AATT_y Cuts at: 1493 2855 3169 3395 3547 5656 1493 Size: 1362 314 226 152 2109 1697 Fragments arranged by size: 2109 1697 1362 314 226 152 AseI AT'TA_AT Cuts at: 160 1032 4414 160 Size: 872 3382 1606 Fragments arranged by size: 3382 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 AvaI C'yCGr_G Cuts at: 1870 1943 2074 3185 1870 Size: 73 131 1111 4545 Fragments arranged by size: 4545 1111 131 73 BamHI G'GATC_C Cuts at: 3190 3557 3190 Size: 367 5493 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3181 4636 618 Size: 325 217 627 1394 1455 1842 Fragments arranged by size: 1842 1455 1394 627 325 217 BanII G_rGCy'C Cuts at: 1391 3179 1391 Size: 1788 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4889 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4051 4085 1903 Size: 34 2114 34 3678 Fragments arranged by size: 3678 2114 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3752 4962 3752 Size: 1210 4650 BclI T'GATC_A Cuts at: 1548 1548 Size: 5860 BfrBI ATG'CAT Cuts at: 2874 2874 Size: 5860 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4471 136 Size: 107 122 71 631 605 2799 1525 Fragments arranged by size: 2799 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5855 5855 Size: 5860 BlpI GC'TnA_GC Cuts at: 2484 2967 3277 2484 Size: 483 310 5067 Fragments arranged by size: 5067 483 310 Bme1580I G_kGCm'C Cuts at: 1790 3921 5077 1790 Size: 2131 1156 2573 Fragments arranged by size: 2573 2131 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5860 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3591 4549 442 Size: 416 53 83 399 24 2174 958 1753 Fragments arranged by size: 2174 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5860 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2499 2499 Size: 5860 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2435 2439 2450 4520 1239 Size: 1196 4 11 2070 2579 Fragments arranged by size: 2579 2070 1196 11 4 Bpu10I CC'TnA_GC Cuts at: 2113 2113 Size: 5860 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3985 4850 5148 3985 Size: 865 298 4697 Fragments arranged by size: 4697 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4523 882 Size: 617 3024 2219 Fragments arranged by size: 3024 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3651 493 Size: 711 2447 2702 Fragments arranged by size: 2702 2447 711 BsaBI GATnn'nnATC Cuts at: 5854 5854 Size: 5860 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2262 2291 3667 4049 Size: 53 83 186 936 729 29 1376 382 Cuts at: 4049 4684 275 Size: 635 1451 Fragments arranged by size: 1451 1376 936 729 635 382 186 83 53 29 BsaWI w'CCGG_w Cuts at: 2185 2710 2838 2928 4293 4691 4965 5112 5523 Size: 525 128 90 1365 398 274 147 411 Cuts at: 5523 2185 Size: 2522 Fragments arranged by size: 2522 1365 525 411 398 274 147 128 90 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2543 2573 1178 Size: 30 165 30 1140 30 4465 Fragments arranged by size: 4465 1140 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2354 1186 Size: 1168 4692 BseYI C'CCAG_C Cuts at: 5063 5559 5063 Size: 496 5364 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2233 2288 1163 Size: 573 497 55 4735 Fragments arranged by size: 4735 573 497 55 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2068 3310 4071 5099 1540 Size: 275 186 67 1242 761 1028 2301 Fragments arranged by size: 2301 1242 1028 761 275 186 67 BsiHKAI G_wGCw'C Cuts at: 3921 4006 5077 3921 Size: 85 1071 4704 Fragments arranged by size: 4704 1071 85 BsmI GAATG_Cn' Cuts at: 2938 3371 3464 2938 Size: 433 93 5334 Fragments arranged by size: 5334 433 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3179 3921 4006 5077 1391 Size: 399 1389 742 85 1071 2174 Fragments arranged by size: 2174 1389 1071 742 399 85 BspEI T'CCGG_A Cuts at: 2928 5523 2928 Size: 2595 3265 BspHI T'CATG_A Cuts at: 2586 3749 5479 2586 Size: 1163 1730 2967 Fragments arranged by size: 2967 1730 1163 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3215 844 Size: 2371 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2278 2461 3747 1394 Size: 429 455 183 1286 3507 Fragments arranged by size: 3507 1286 455 429 183 BsrDI GCAATG_nn' Cuts at: 65 1248 2943 3115 4355 4529 65 Size: 1183 1695 172 1240 174 1396 Fragments arranged by size: 1695 1396 1240 1183 174 172 BsrFI r'CCGG_y Cuts at: 1700 1810 2386 4504 4691 1700 Size: 110 576 2118 187 2869 Fragments arranged by size: 2869 2118 576 187 110 BsrGI T'GTAC_A Cuts at: 96 3141 96 Size: 3045 2815 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5860 BssSI C'ACGA_G Cuts at: 2273 2471 3920 4932 2273 Size: 198 1449 1012 3201 Fragments arranged by size: 3201 1449 1012 198 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5860 BstBI TT'CG_AA Cuts at: 4664 4664 Size: 5860 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5860 BstYI r'GATC_y Cuts at: 1972 3190 3557 3942 3959 5411 5497 5509 5855 Size: 1218 367 385 17 1452 86 12 346 Cuts at: 5855 1972 Size: 1977 Fragments arranged by size: 1977 1452 1218 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2326 2400 2439 2547 513 Size: 552 1261 74 39 108 3826 Fragments arranged by size: 3826 1261 552 108 74 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5121 Fragments arranged by size: 5121 683 56 BtsI GCAGTG_nn' Cuts at: 3376 4170 4190 3376 Size: 794 20 5046 Fragments arranged by size: 5046 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5860 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2041 2076 529 Size: 35 1477 35 4313 Fragments arranged by size: 4313 1477 35 35 DraI TTT'AAA Cuts at: 3164 3164 Size: 5860 DraIII CAC_nnn'GTG Cuts at: 2906 2906 Size: 5860 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4867 809 Size: 507 3551 1802 Fragments arranged by size: 3551 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2436 2544 3307 Size: 54 1425 50 275 18 606 108 763 Cuts at: 3307 4196 5825 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 763 606 275 108 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3307 1537 Size: 275 1495 4090 Fragments arranged by size: 4090 1495 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2588 3790 1353 Size: 1235 1202 3423 Fragments arranged by size: 3423 1235 1202 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4447 4821 4967 387 Size: 1064 138 2858 374 146 1280 Fragments arranged by size: 2858 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2249 2435 2439 2450 3923 4520 5307 Size: 393 617 186 4 11 1473 597 787 Cuts at: 5307 5575 1239 Size: 268 1524 Fragments arranged by size: 1524 1473 787 617 597 393 268 186 11 4 EcoO109I rG'GnC_Cy Cuts at: 2148 2282 2520 2148 Size: 134 238 5488 Fragments arranged by size: 5488 238 134 EcoRI G'AATT_C Cuts at: 3169 3547 3169 Size: 378 5482 EcoRV GAT'ATC Cuts at: 2044 3002 3157 2044 Size: 958 155 4747 Fragments arranged by size: 4747 958 155 FspI TGC'GCA Cuts at: 4366 4366 Size: 5860 HaeII r_GCGC'y Cuts at: 1678 2378 5007 1678 Size: 700 2629 2531 Fragments arranged by size: 2629 2531 700 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2057 2089 2096 2128 4501 Size: 32 135 32 485 32 7 32 2373 Cuts at: 4501 4533 4575 4607 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2373 485 135 42 32 32 32 32 32 32 7 HincII GTy'rAC Cuts at: 669 1657 1938 2248 3204 3450 669 Size: 988 281 310 956 246 3079 Fragments arranged by size: 3079 988 956 310 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5860 HpaI GTT'AAC Cuts at: 3450 3450 Size: 5860 KpnI G_GTAC'C Cuts at: 3185 3185 Size: 5860 MfeI C'AATT_G Cuts at: 3459 3459 Size: 5860 MluI A'CGCG_T Cuts at: 5736 5736 Size: 5860 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4974 5158 1305 Size: 3669 184 2007 Fragments arranged by size: 3669 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2546 10 Size: 54 1425 343 714 3324 Fragments arranged by size: 3324 1425 714 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3818 4177 4336 518 Size: 648 2652 359 159 2042 Fragments arranged by size: 2652 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2402 2413 2441 2570 2647 3276 3953 5101 Size: 796 11 28 129 77 629 677 1148 Cuts at: 5101 5346 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 796 677 629 245 129 77 28 11 NaeI GCC'GGC Cuts at: 2388 2388 Size: 5860 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5308 NdeI CA'TA_TG Cuts at: 387 387 Size: 5860 NgoMIV G'CCGG_C Cuts at: 2386 2386 Size: 5860 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5860 NotI GC'GGCC_GC Cuts at: 3307 3307 Size: 5860 NsiI A_TGCA'T Cuts at: 2876 2876 Size: 5860 NspI r_CATG'y Cuts at: 1132 1229 3218 4763 1132 Size: 97 1989 1545 2229 Fragments arranged by size: 2229 1989 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5860 PciI A'CATG_T Cuts at: 4759 4759 Size: 5860 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5860 PmeI GTTT'AAAC Cuts at: 3164 3164 Size: 5860 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3929 3961 5442 5474 3929 Size: 32 1481 32 4315 Fragments arranged by size: 4315 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2148 2520 2148 Size: 372 5488 PshAI GACnn'nnGTC Cuts at: 1613 2153 1613 Size: 540 5320 PsiI TTA'TAA Cuts at: 3430 3430 Size: 5860 PstI C_TGCA'G Cuts at: 830 3212 830 Size: 2382 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5860 PvuII CAG'CTG Cuts at: 2413 2570 2647 2413 Size: 157 77 5626 Fragments arranged by size: 5626 157 77 SacII CC_GC'GG Cuts at: 2403 2442 2403 Size: 39 5821 SalI G'TCGA_C Cuts at: 1936 3202 1936 Size: 1266 4594 SanDI GG'GwC_CC Cuts at: 2520 2520 Size: 5860 SbfI CC_TGCA'GG Cuts at: 3212 3212 Size: 5860 ScaI AGT'ACT Cuts at: 4108 5820 4108 Size: 1712 4148 SfcI C'TryA_G Cuts at: 826 1045 3075 3208 4343 5024 5215 826 Size: 219 2030 133 1135 681 191 1471 Fragments arranged by size: 2030 1471 1135 681 219 191 133 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5860 SmaI CCC'GGG Cuts at: 1872 3187 1872 Size: 1315 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3964 4865 5127 820 Size: 197 926 2021 901 262 1553 Fragments arranged by size: 2021 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3651 493 Size: 3158 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5860 SphI G_CATG'C Cuts at: 1132 3218 1132 Size: 2086 3774 SspI AAT'ATT Cuts at: 5 52 3784 5 Size: 47 3732 2081 Fragments arranged by size: 3732 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5860 StyI C'CwwG_G Cuts at: 513 1065 3299 513 Size: 552 2234 3074 Fragments arranged by size: 3074 2234 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3887 3904 4057 4242 4687 325 Size: 1255 2307 17 153 185 445 1498 Fragments arranged by size: 2307 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3141 4106 5818 Size: 274 80 33 51 1443 1164 965 1712 Cuts at: 5818 96 Size: 138 Fragments arranged by size: 1712 1443 1164 965 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2543 2575 5820 5852 2543 Size: 32 3245 32 2551 Fragments arranged by size: 3245 2551 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5860 XbaI T'CTAG_A Cuts at: 3196 3196 Size: 5860 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2513 2513 Size: 5860 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5860 XmaI C'CCGG_G Cuts at: 1870 3185 1870 Size: 1315 4545 XmnI GAAnn'nnTTC Cuts at: 3989 3989 Size: 5860 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2292 3668 276 Size: 53 83 186 936 758 1376 2468 Fragments arranged by size: 2468 1376 936 758 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI FalI FseI FspAI KasI NarI NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI