(Circular) (Six-base) MAPSORT of: pFN21AB4113.seq Check: 9098 from: 1 to: 6277 pFN21AB4113 6277 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4087 278 Size: 53 83 186 936 2551 2468 Fragments arranged by size: 2551 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3321 3534 3620 1937 Size: 1384 213 86 4594 Fragments arranged by size: 4594 1384 213 86 Acc65I G'GTAC_C Cuts at: 3598 3598 Size: 6277 AclI AA'CG_TT Cuts at: 1563 4404 4777 1563 Size: 2841 373 3063 Fragments arranged by size: 3063 2841 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2069 2427 3057 3456 4340 5724 5992 1632 Size: 437 358 630 399 884 1384 268 1917 Fragments arranged by size: 1917 1384 884 630 437 399 358 268 AfeI AGC'GCT Cuts at: 2042 2042 Size: 6277 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6080 AflIII A'CryG_T Cuts at: 1203 5176 6153 1203 Size: 3973 977 1327 Fragments arranged by size: 3973 1327 977 AgeI A'CCGG_T Cuts at: 3336 5108 3336 Size: 1772 4505 AhdI GACnn_n'nnGTC Cuts at: 5006 5006 Size: 6277 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6277 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6277 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2100 2132 2100 Size: 32 6245 AlwNI CAG_nnn'CTG Cuts at: 4020 5592 4020 Size: 1572 4705 ApaLI G'TGCA_C Cuts at: 4334 5490 4334 Size: 1156 5121 ApoI r'AATT_y Cuts at: 1493 2150 2175 2706 3586 3812 3964 6073 1493 Size: 657 25 531 880 226 152 2109 1697 Fragments arranged by size: 2109 1697 880 657 531 226 152 25 AseI AT'TA_AT Cuts at: 160 1032 2213 4831 160 Size: 872 1181 2618 1606 Fragments arranged by size: 2618 1606 1181 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6277 AvaI C'yCGr_G Cuts at: 1870 1943 3602 1870 Size: 73 1659 4545 Fragments arranged by size: 4545 1659 73 BamHI G'GATC_C Cuts at: 3607 3974 3607 Size: 367 5910 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3598 5053 618 Size: 325 217 627 1811 1455 1842 Fragments arranged by size: 1842 1811 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3596 1391 Size: 2205 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2832 3495 928 Size: 971 933 663 3710 Fragments arranged by size: 3710 971 933 663 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4468 4502 1903 Size: 34 2531 34 3678 Fragments arranged by size: 3678 2531 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2272 4169 5379 2272 Size: 1897 1210 3170 Fragments arranged by size: 3170 1897 1210 BclI T'GATC_A Cuts at: 1548 3309 1548 Size: 1761 4516 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4888 136 Size: 107 122 71 631 605 3216 1525 Fragments arranged by size: 3216 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2434 6272 2434 Size: 3838 2439 BlpI GC'TnA_GC Cuts at: 3694 3694 Size: 6277 Bme1580I G_kGCm'C Cuts at: 1790 3240 4338 5494 1790 Size: 1450 1098 1156 2573 Fragments arranged by size: 2573 1450 1156 1098 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6277 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2068 2839 2886 Size: 416 53 83 399 24 651 771 47 Cuts at: 2886 4008 4966 442 Size: 1122 958 1753 Fragments arranged by size: 1753 1122 958 771 651 416 399 83 53 47 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6277 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3084 3084 Size: 6277 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2190 3434 4937 1239 Size: 951 1244 1503 2579 Fragments arranged by size: 2579 1503 1244 951 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4402 5267 5565 4402 Size: 865 298 5114 Fragments arranged by size: 5114 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4940 882 Size: 617 3441 2219 Fragments arranged by size: 3441 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4068 493 Size: 711 2864 2702 Fragments arranged by size: 2864 2702 711 BsaBI GATnn'nnATC Cuts at: 6271 6271 Size: 6277 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4084 4466 5101 275 Size: 53 83 186 936 2551 382 635 1451 Fragments arranged by size: 2551 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2264 3336 4710 5108 5382 5529 5940 2264 Size: 1072 1374 398 274 147 411 2601 Fragments arranged by size: 2601 1374 1072 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2100 2130 1178 Size: 30 165 30 697 30 5325 Fragments arranged by size: 5325 697 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6277 BseYI C'CCAG_C Cuts at: 2519 2804 2848 5480 5976 2519 Size: 285 44 2632 496 2820 Fragments arranged by size: 2820 2632 496 285 44 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2229 1163 Size: 573 493 5211 Fragments arranged by size: 5211 573 493 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3727 4488 5516 1540 Size: 275 186 1726 761 1028 2301 Fragments arranged by size: 2301 1726 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4338 4423 5494 4338 Size: 85 1071 5121 Fragments arranged by size: 5121 1071 85 BsiWI C'GTAC_G Cuts at: 2929 2929 Size: 6277 BsmI GAATG_Cn' Cuts at: 3788 3881 3788 Size: 93 6184 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3240 3596 4338 4423 5494 1391 Size: 399 1450 356 742 85 1071 2174 Fragments arranged by size: 2174 1450 1071 742 399 356 85 BspEI T'CCGG_A Cuts at: 2264 5940 2264 Size: 3676 2601 BspHI T'CATG_A Cuts at: 2565 2988 4166 5896 2565 Size: 423 1178 1730 2946 Fragments arranged by size: 2946 1730 1178 423 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3632 844 Size: 2788 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4164 1394 Size: 429 2341 3507 Fragments arranged by size: 3507 2341 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2313 3312 4772 4946 65 Size: 1183 1065 999 1460 174 1396 Fragments arranged by size: 1460 1396 1183 1065 999 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2014 3336 4921 5108 1700 Size: 110 204 1322 1585 187 2869 Fragments arranged by size: 2869 1585 1322 204 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6277 BssHII G'CGCG_C Cuts at: 1925 2281 1925 Size: 356 5921 BssSI C'ACGA_G Cuts at: 4337 5349 4337 Size: 1012 5265 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6277 BstBI TT'CG_AA Cuts at: 5081 5081 Size: 6277 BstEII G'GTnAC_C Cuts at: 1184 3253 1184 Size: 2069 4208 BstXI CCAn_nnnn'nTGG Cuts at: 2962 2962 Size: 6277 BstYI r'GATC_y Cuts at: 1972 2434 3607 3974 4359 4376 5828 5914 5926 Size: 462 1173 367 385 17 1452 86 12 Cuts at: 5926 6272 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1173 462 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2076 2082 2955 3258 3279 3348 Size: 552 939 72 6 873 303 21 69 Cuts at: 3348 513 Size: 3442 Fragments arranged by size: 3442 939 873 552 303 72 69 21 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5538 Fragments arranged by size: 5538 683 56 BtsI GCAGTG_nn' Cuts at: 3011 3135 3793 4587 4607 3011 Size: 124 658 794 20 4681 Fragments arranged by size: 4681 794 658 124 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6277 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2296 2331 3460 3495 529 Size: 35 1732 35 1129 35 3311 Fragments arranged by size: 3311 1732 1129 35 35 35 DraI TTT'AAA Cuts at: 3581 3581 Size: 6277 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2548 5284 809 Size: 507 1232 2736 1802 Fragments arranged by size: 2736 1802 1232 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2079 2862 3204 Size: 54 1425 50 275 18 249 783 342 Cuts at: 3204 3724 4613 6242 8 Size: 520 889 1629 43 Fragments arranged by size: 1629 1425 889 783 520 342 275 249 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3724 1537 Size: 275 1912 4090 Fragments arranged by size: 4090 1912 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2541 3001 4207 1353 Size: 1188 460 1206 3423 Fragments arranged by size: 3423 1206 1188 460 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4864 5238 5384 387 Size: 1064 138 3275 374 146 1280 Fragments arranged by size: 3275 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2190 2427 3057 3434 3456 4340 Size: 393 437 121 237 630 377 22 884 Cuts at: 4340 4937 5724 5992 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 884 787 630 597 437 393 377 268 237 121 22 EcoRI G'AATT_C Cuts at: 3586 3964 3586 Size: 378 5899 FspI TGC'GCA Cuts at: 4783 4783 Size: 6277 HaeII r_GCGC'y Cuts at: 1678 2044 5424 1678 Size: 366 3380 2531 Fragments arranged by size: 3380 2531 366 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3416 3448 3536 3568 4918 Size: 32 135 32 1844 32 88 32 1350 Cuts at: 4918 4950 4992 5024 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1844 1350 135 88 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3322 3355 3442 3621 3867 669 Size: 988 281 1384 33 87 179 246 3079 Fragments arranged by size: 3079 1384 988 281 246 179 87 33 HindIII A'AGCT_T Cuts at: 748 748 Size: 6277 HpaI GTT'AAC Cuts at: 3867 3867 Size: 6277 KpnI G_GTAC'C Cuts at: 3602 3602 Size: 6277 MfeI C'AATT_G Cuts at: 3876 3876 Size: 6277 MluI A'CGCG_T Cuts at: 6153 6153 Size: 6277 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2280 5391 5575 1305 Size: 975 3111 184 2007 Fragments arranged by size: 3111 2007 975 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2864 10 Size: 54 1425 343 1032 3423 Fragments arranged by size: 3423 1425 1032 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2960 4235 4594 4753 518 Size: 648 1794 1275 359 159 2042 Fragments arranged by size: 2042 1794 1275 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2084 2561 2911 3030 3137 3693 4370 5518 Size: 478 477 350 119 107 556 677 1148 Cuts at: 5518 5763 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 556 478 477 350 245 119 107 NcoI C'CATG_G Cuts at: 513 1065 2004 2955 3258 3348 513 Size: 552 939 951 303 90 3442 Fragments arranged by size: 3442 951 939 552 303 90 NdeI CA'TA_TG Cuts at: 387 387 Size: 6277 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6277 NotI GC'GGCC_GC Cuts at: 3724 3724 Size: 6277 NspI r_CATG'y Cuts at: 1132 1229 2620 3635 5180 1132 Size: 97 1391 1015 1545 2229 Fragments arranged by size: 2229 1545 1391 1015 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6277 PciI A'CATG_T Cuts at: 5176 5176 Size: 6277 PflMI CCAn_nnn'nTGG Cuts at: 1108 2374 1108 Size: 1266 5011 PfoI T'CCnGG_A Cuts at: 2167 3330 2167 Size: 1163 5114 PmeI GTTT'AAAC Cuts at: 3581 3581 Size: 6277 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2100 2132 4346 4378 5859 5891 2100 Size: 32 2214 32 1481 32 2486 Fragments arranged by size: 2486 2214 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3350 1613 Size: 1737 4540 PsiI TTA'TAA Cuts at: 3847 3847 Size: 6277 PstI C_TGCA'G Cuts at: 830 3629 830 Size: 2799 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6277 PvuII CAG'CTG Cuts at: 2561 2561 Size: 6277 SacII CC_GC'GG Cuts at: 2085 2085 Size: 6277 SalI G'TCGA_C Cuts at: 1936 3320 3619 1936 Size: 1384 299 4594 Fragments arranged by size: 4594 1384 299 SbfI CC_TGCA'GG Cuts at: 3629 3629 Size: 6277 ScaI AGT'ACT Cuts at: 4525 6237 4525 Size: 1712 4565 SexAI A'CCwGG_T Cuts at: 3048 3048 Size: 6277 SfcI C'TryA_G Cuts at: 826 1045 3625 4760 5441 5632 826 Size: 219 2580 1135 681 191 1471 Fragments arranged by size: 2580 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6277 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6277 SmaI CCC'GGG Cuts at: 1872 3604 1872 Size: 1732 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4381 5282 5544 820 Size: 197 926 2438 901 262 1553 Fragments arranged by size: 2438 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4068 493 Size: 3575 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6277 SphI G_CATG'C Cuts at: 1132 3635 1132 Size: 2503 3774 SspI AAT'ATT Cuts at: 5 52 2838 4201 5 Size: 47 2786 1363 2081 Fragments arranged by size: 2786 2081 1363 47 StuI AGG'CCT Cuts at: 1517 2474 1517 Size: 957 5320 StyI C'CwwG_G Cuts at: 513 1065 2004 2955 3258 3348 3361 3462 3716 Size: 552 939 951 303 90 13 101 254 Cuts at: 3716 513 Size: 3074 Fragments arranged by size: 3074 951 939 552 303 254 101 90 13 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2050 2366 2381 3575 4304 4321 4474 Size: 1255 470 316 15 1194 729 17 153 Cuts at: 4474 4659 5104 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1194 729 470 445 316 185 153 17 15 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2487 4523 6235 Size: 274 80 33 51 1443 510 2036 1712 Cuts at: 6235 96 Size: 138 Fragments arranged by size: 2036 1712 1443 510 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6237 6269 6237 Size: 32 6245 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6277 XbaI T'CTAG_A Cuts at: 2143 3613 2143 Size: 1470 4807 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2110 3409 2110 Size: 1299 4978 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6277 XmaI C'CCGG_G Cuts at: 1870 3602 1870 Size: 1732 4545 XmnI GAAnn'nnTTC Cuts at: 2042 2450 2942 4406 2042 Size: 408 492 1464 3913 Fragments arranged by size: 3913 1464 492 408 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4085 276 Size: 53 83 186 936 2551 2468 Fragments arranged by size: 2551 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI