(Circular) (Six-base) MAPSORT of: pFN21AB8290.seq Check: 2353 from: 1 to: 6388 pFN21AB8290 6388 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4198 278 Size: 53 83 186 936 2662 2468 Fragments arranged by size: 2662 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3731 1937 Size: 1794 4594 Acc65I G'GTAC_C Cuts at: 3566 3709 3566 Size: 143 6245 AclI AA'CG_TT Cuts at: 1563 3687 4515 4888 1563 Size: 2124 828 373 3063 Fragments arranged by size: 3063 2124 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2765 3221 3405 4451 5835 6103 1632 Size: 1133 456 184 1046 1384 268 1917 Fragments arranged by size: 1917 1384 1133 1046 456 268 184 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6191 AflIII A'CryG_T Cuts at: 1203 2071 5287 6264 1203 Size: 868 3216 977 1327 Fragments arranged by size: 3216 1327 977 868 AgeI A'CCGG_T Cuts at: 5219 5219 Size: 6388 AhdI GACnn_n'nnGTC Cuts at: 5117 5117 Size: 6388 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6388 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6388 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3070 3102 3070 Size: 32 6356 AlwNI CAG_nnn'CTG Cuts at: 2897 4131 5703 2897 Size: 1234 1572 3582 Fragments arranged by size: 3582 1572 1234 ApaLI G'TGCA_C Cuts at: 4445 5601 4445 Size: 1156 5232 ApoI r'AATT_y Cuts at: 1493 2039 3697 3923 4075 6184 1493 Size: 546 1658 226 152 2109 1697 Fragments arranged by size: 2109 1697 1658 546 226 152 AseI AT'TA_AT Cuts at: 160 1032 4942 160 Size: 872 3910 1606 Fragments arranged by size: 3910 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6388 AvaI C'yCGr_G Cuts at: 1870 1943 2767 3713 1870 Size: 73 824 946 4545 Fragments arranged by size: 4545 946 824 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3508 3541 3508 Size: 33 6355 BamHI G'GATC_C Cuts at: 3718 4085 3718 Size: 367 6021 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3307 3566 3709 5164 618 Size: 325 217 627 1520 259 143 1455 1842 Fragments arranged by size: 1842 1520 1455 627 325 259 217 143 BanII G_rGCy'C Cuts at: 1391 3707 1391 Size: 2316 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2647 2787 2928 928 Size: 971 748 140 141 4388 Fragments arranged by size: 4388 971 748 141 140 BbvCI CC'TCA_GC Cuts at: 3473 3473 Size: 6388 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4579 4613 1903 Size: 34 2642 34 3678 Fragments arranged by size: 3678 2642 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2692 3187 3529 4280 5490 2692 Size: 495 342 751 1210 3590 Fragments arranged by size: 3590 1210 751 495 342 BclI T'GATC_A Cuts at: 1548 1548 Size: 6388 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4999 136 Size: 107 122 71 631 605 3327 1525 Fragments arranged by size: 3327 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6383 6383 Size: 6388 BlpI GC'TnA_GC Cuts at: 3805 3805 Size: 6388 Bme1580I G_kGCm'C Cuts at: 1790 4449 5605 1790 Size: 2659 1156 2573 Fragments arranged by size: 2659 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6388 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3318 3516 4119 Size: 416 53 83 399 24 1901 198 603 Cuts at: 4119 5077 442 Size: 958 1753 Fragments arranged by size: 1901 1753 958 603 416 399 198 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3579 1056 Size: 2523 3865 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2471 2471 Size: 6388 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2601 2670 2730 3069 3114 5048 1239 Size: 1362 69 60 339 45 1934 2579 Fragments arranged by size: 2579 1934 1362 339 69 60 45 Bpu10I CC'TnA_GC Cuts at: 3347 3473 3347 Size: 126 6262 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2648 3396 4513 5378 5676 2648 Size: 748 1117 865 298 3360 Fragments arranged by size: 3360 1117 865 748 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2793 3438 5051 882 Size: 617 1294 645 1613 2219 Fragments arranged by size: 2219 1613 1294 645 617 BsaAI yAC'GTr Cuts at: 493 1204 2554 4179 493 Size: 711 1350 1625 2702 Fragments arranged by size: 2702 1625 1350 711 BsaBI GATnn'nnATC Cuts at: 6382 6382 Size: 6388 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2389 4195 4577 5212 Size: 53 83 186 936 856 1806 382 635 Cuts at: 5212 275 Size: 1451 Fragments arranged by size: 1806 1451 936 856 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4821 5219 5493 5640 6051 4821 Size: 398 274 147 411 5158 Fragments arranged by size: 5158 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3064 3070 3094 3100 1178 Size: 30 165 30 1661 6 24 6 4466 Fragments arranged by size: 4466 1661 165 30 30 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6388 BseYI C'CCAG_C Cuts at: 2482 3024 5591 6087 2482 Size: 542 2567 496 2783 Fragments arranged by size: 2783 2567 542 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2576 3348 1163 Size: 573 840 772 4203 Fragments arranged by size: 4203 840 772 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3838 4599 5627 1540 Size: 275 186 1837 761 1028 2301 Fragments arranged by size: 2301 1837 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2286 3017 4449 4534 5605 2286 Size: 731 1432 85 1071 3069 Fragments arranged by size: 3069 1432 1071 731 85 BsmI GAATG_Cn' Cuts at: 3899 3992 3899 Size: 93 6295 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2286 3017 3707 4449 4534 5605 1391 Size: 399 496 731 690 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 731 690 496 399 85 BspEI T'CCGG_A Cuts at: 6051 6051 Size: 6388 BspHI T'CATG_A Cuts at: 2457 2755 4277 6007 2457 Size: 298 1522 1730 2838 Fragments arranged by size: 2838 1730 1522 298 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2112 3743 844 Size: 1268 1631 3489 Fragments arranged by size: 3489 1631 1268 BsrBI CCG'CTC Cuts at: 1394 1823 2452 4275 1394 Size: 429 629 1823 3507 Fragments arranged by size: 3507 1823 629 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2470 4883 5057 65 Size: 1183 1222 2413 174 1396 Fragments arranged by size: 2413 1396 1222 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3619 5032 5219 1700 Size: 110 1809 1413 187 2869 Fragments arranged by size: 2869 1809 1413 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6388 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6388 BssSI C'ACGA_G Cuts at: 4448 5460 4448 Size: 1012 5376 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6388 BstBI TT'CG_AA Cuts at: 5192 5192 Size: 6388 BstEII G'GTnAC_C Cuts at: 1184 2621 1184 Size: 1437 4951 BstXI CCAn_nnnn'nTGG Cuts at: 2708 2708 Size: 6388 BstYI r'GATC_y Cuts at: 1972 2395 3718 4085 4470 4487 5939 6025 6037 Size: 423 1323 367 385 17 1452 86 12 Cuts at: 6037 6383 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1323 423 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3189 3400 3189 Size: 211 6177 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5836 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5649 Fragments arranged by size: 5649 683 56 BtsI GCAGTG_nn' Cuts at: 3265 3336 3904 4698 4718 3265 Size: 71 568 794 20 4935 Fragments arranged by size: 4935 794 568 71 20 ClaI AT'CG_AT Cuts at: 1554 3421 1554 Size: 1867 4521 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6353 DraI TTT'AAA Cuts at: 2131 3692 2131 Size: 1561 4827 DraIII CAC_nnn'GTG Cuts at: 2492 3062 2492 Size: 570 5818 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5395 809 Size: 507 4079 1802 Fragments arranged by size: 4079 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3286 3621 3835 Size: 54 1425 50 275 18 1456 335 214 Cuts at: 3835 4724 6353 8 Size: 889 1629 43 Fragments arranged by size: 1629 1456 1425 889 335 275 214 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3835 1537 Size: 275 2023 4090 Fragments arranged by size: 4090 2023 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4318 1353 Size: 2965 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4975 5349 5495 387 Size: 1064 138 3386 374 146 1280 Fragments arranged by size: 3386 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2601 2670 2730 2765 3069 3114 3221 Size: 393 969 69 60 35 304 45 107 Cuts at: 3221 3405 4451 5048 5835 6103 1239 Size: 184 1046 597 787 268 1524 Fragments arranged by size: 1524 1046 969 787 597 393 304 268 184 107 69 60 45 35 EcoNI CCTnn'n_nnAGG Cuts at: 2369 2369 Size: 6388 EcoRI G'AATT_C Cuts at: 3697 4075 3697 Size: 378 6010 EcoRV GAT'ATC Cuts at: 3170 3170 Size: 6388 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2201 3140 2201 Size: 939 5449 FspI TGC'GCA Cuts at: 2246 4894 2246 Size: 2648 3740 HaeII r_GCGC'y Cuts at: 1678 5535 1678 Size: 3857 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 1999 2031 2750 2782 3481 Size: 32 135 32 427 32 719 32 699 Cuts at: 3481 3513 5029 5061 5103 5135 1373 Size: 32 1516 32 42 32 2626 Fragments arranged by size: 2626 1516 719 699 427 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2716 3732 3978 669 Size: 988 281 778 1016 246 3079 Fragments arranged by size: 3079 1016 988 778 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6388 HpaI GTT'AAC Cuts at: 3978 3978 Size: 6388 KpnI G_GTAC'C Cuts at: 3570 3713 3570 Size: 143 6245 MfeI C'AATT_G Cuts at: 2082 2724 3987 2082 Size: 642 1263 4483 Fragments arranged by size: 4483 1263 642 MluI A'CGCG_T Cuts at: 6264 6264 Size: 6388 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2966 3134 3182 5502 5686 1305 Size: 1661 168 48 2320 184 2007 Fragments arranged by size: 2320 2007 1661 184 168 48 MscI TGG'CCA Cuts at: 10 64 1489 1832 3288 10 Size: 54 1425 343 1456 3110 Fragments arranged by size: 3110 1456 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2076 3324 4346 4705 4864 518 Size: 648 910 1248 1022 359 159 2042 Fragments arranged by size: 2042 1248 1022 910 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2894 3472 3804 4481 5629 5874 1606 Size: 1288 578 332 677 1148 245 2120 Fragments arranged by size: 2120 1288 1148 677 578 332 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5836 NdeI CA'TA_TG Cuts at: 387 387 Size: 6388 NheI G'CTAG_C Cuts at: 1052 3575 1052 Size: 2523 3865 NotI GC'GGCC_GC Cuts at: 3835 3835 Size: 6388 NspI r_CATG'y Cuts at: 1132 1229 2075 3746 5291 1132 Size: 97 846 1671 1545 2229 Fragments arranged by size: 2229 1671 1545 846 97 PasI CC'CwG_GG Cuts at: 1348 2701 1348 Size: 1353 5035 PciI A'CATG_T Cuts at: 2071 5287 2071 Size: 3216 3172 PflMI CCAn_nnn'nTGG Cuts at: 1108 2897 1108 Size: 1789 4599 PfoI T'CCnGG_A Cuts at: 3037 3037 Size: 6388 PmeI GTTT'AAAC Cuts at: 3692 3692 Size: 6388 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3070 3102 4457 4489 5970 6002 3070 Size: 32 1355 32 1481 32 3456 Fragments arranged by size: 3456 1481 1355 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6388 PsiI TTA'TAA Cuts at: 3958 3958 Size: 6388 PstI C_TGCA'G Cuts at: 830 2109 3740 830 Size: 1279 1631 3478 Fragments arranged by size: 3478 1631 1279 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6388 PvuII CAG'CTG Cuts at: 2894 3472 2894 Size: 578 5810 SalI G'TCGA_C Cuts at: 1936 3730 1936 Size: 1794 4594 SbfI CC_TGCA'GG Cuts at: 2109 3740 2109 Size: 1631 4757 ScaI AGT'ACT Cuts at: 2126 4636 6348 2126 Size: 2510 1712 2166 Fragments arranged by size: 2510 2166 1712 SfcI C'TryA_G Cuts at: 826 1045 2105 2859 3736 4871 5552 5743 826 Size: 219 1060 754 877 1135 681 191 1471 Fragments arranged by size: 1471 1135 1060 877 754 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6388 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6388 SmaI CCC'GGG Cuts at: 1872 3715 1872 Size: 1843 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2627 3411 4492 5393 5655 820 Size: 197 926 684 784 1081 901 262 1553 Fragments arranged by size: 1553 1081 926 901 784 684 262 197 SnaBI TAC'GTA Cuts at: 493 4179 493 Size: 3686 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6388 SphI G_CATG'C Cuts at: 1132 3746 1132 Size: 2614 3774 SspI AAT'ATT Cuts at: 5 52 4312 5 Size: 47 4260 2081 Fragments arranged by size: 4260 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6388 StyI C'CwwG_G Cuts at: 513 1065 2430 3827 513 Size: 552 1365 1397 3074 Fragments arranged by size: 3074 1397 1365 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2061 3625 4415 4432 4585 4770 5215 Size: 1255 481 1564 790 17 153 185 445 Cuts at: 5215 325 Size: 1498 Fragments arranged by size: 1564 1498 1255 790 481 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2124 4634 6346 Size: 274 80 33 51 1443 147 2510 1712 Cuts at: 6346 96 Size: 138 Fragments arranged by size: 2510 1712 1443 274 147 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2478 2510 3105 3137 6348 6380 2478 Size: 32 595 32 3211 32 2486 Fragments arranged by size: 3211 2486 595 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2381 1316 Size: 1065 5323 XbaI T'CTAG_A Cuts at: 3724 3724 Size: 6388 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3424 3424 Size: 6388 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6388 XmaI C'CCGG_G Cuts at: 1870 3713 1870 Size: 1843 4545 XmnI GAAnn'nnTTC Cuts at: 4517 4517 Size: 6388 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4196 276 Size: 53 83 186 936 2662 2468 Fragments arranged by size: 2662 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI