(Circular) (Six-base) MAPSORT of: pFN21AB3486.seq Check: 6517 from: 1 to: 5809 pFN21AB3486 5809 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2626 2626 Size: 5809 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3000 3619 278 Size: 53 83 186 936 1464 619 2468 Fragments arranged by size: 2468 1464 936 619 186 83 53 AccI GT'mk_AC Cuts at: 1937 3152 1937 Size: 1215 4594 Acc65I G'GTAC_C Cuts at: 3130 3130 Size: 5809 AclI AA'CG_TT Cuts at: 1563 3936 4309 1563 Size: 2373 373 3063 Fragments arranged by size: 3063 2373 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2042 2477 2498 3872 5256 5524 1632 Size: 410 435 21 1374 1384 268 1917 Fragments arranged by size: 1917 1384 1374 435 410 268 21 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5612 AflIII A'CryG_T Cuts at: 1203 4708 5685 1203 Size: 3505 977 1327 Fragments arranged by size: 3505 1327 977 AgeI A'CCGG_T Cuts at: 4640 4640 Size: 5809 AhdI GACnn_n'nnGTC Cuts at: 3055 4538 3055 Size: 1483 4326 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5809 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5809 AlwNI CAG_nnn'CTG Cuts at: 2440 2563 2701 3552 5124 2440 Size: 123 138 851 1572 3125 Fragments arranged by size: 3125 1572 851 138 123 ApaLI G'TGCA_C Cuts at: 3866 5022 3866 Size: 1156 4653 ApoI r'AATT_y Cuts at: 1493 3118 3344 3496 5605 1493 Size: 1625 226 152 2109 1697 Fragments arranged by size: 2109 1697 1625 226 152 AseI AT'TA_AT Cuts at: 160 1032 4363 160 Size: 872 3331 1606 Fragments arranged by size: 3331 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5809 AvaI C'yCGr_G Cuts at: 1870 1943 2781 2960 3134 1870 Size: 73 838 179 174 4545 Fragments arranged by size: 4545 838 179 174 73 BamHI G'GATC_C Cuts at: 3139 3506 3139 Size: 367 5442 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3130 4585 618 Size: 325 217 627 1343 1455 1842 Fragments arranged by size: 1842 1455 1343 627 325 217 BanII G_rGCy'C Cuts at: 1391 2790 2961 3128 1391 Size: 1399 171 167 4072 Fragments arranged by size: 4072 1399 171 167 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4838 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2844 2878 4000 4034 1903 Size: 34 907 34 1122 34 3678 Fragments arranged by size: 3678 1122 907 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3701 4911 3701 Size: 1210 4599 BclI T'GATC_A Cuts at: 1548 1548 Size: 5809 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2198 3060 4420 Size: 107 122 71 631 605 526 862 1360 Cuts at: 4420 136 Size: 1525 Fragments arranged by size: 1525 1360 862 631 605 526 122 107 71 BglII A'GATC_T Cuts at: 5804 5804 Size: 5809 BlpI GC'TnA_GC Cuts at: 2202 3074 3226 2202 Size: 872 152 4785 Fragments arranged by size: 4785 872 152 Bme1580I G_kGCm'C Cuts at: 1790 3099 3870 5026 1790 Size: 1309 771 1156 2573 Fragments arranged by size: 2573 1309 1156 771 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5809 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3540 4498 442 Size: 416 53 83 399 24 2123 958 1753 Fragments arranged by size: 2123 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5809 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2417 2507 2585 2639 4469 1239 Size: 1178 90 78 54 1830 2579 Fragments arranged by size: 2579 1830 1178 90 78 54 Bpu10I CC'TnA_GC Cuts at: 2757 2808 2757 Size: 51 5758 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2708 3934 4799 5097 2708 Size: 1226 865 298 3420 Fragments arranged by size: 3420 1226 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4472 882 Size: 617 2973 2219 Fragments arranged by size: 2973 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3082 3600 493 Size: 711 1878 518 2702 Fragments arranged by size: 2702 1878 711 518 BsaBI GATnn'nnATC Cuts at: 5803 5803 Size: 5809 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2997 3616 3998 4633 Size: 53 83 186 936 1464 619 382 635 Cuts at: 4633 275 Size: 1451 Fragments arranged by size: 1464 1451 936 635 619 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2892 4242 4640 4914 5061 5472 2892 Size: 1350 398 274 147 411 3229 Fragments arranged by size: 3229 1350 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5584 Fragments arranged by size: 5584 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2561 2591 1186 Size: 1375 30 4404 Fragments arranged by size: 4404 1375 30 BseYI C'CCAG_C Cuts at: 2188 2314 2977 5012 5508 2188 Size: 126 663 2035 496 2489 Fragments arranged by size: 2489 2035 663 496 126 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2714 2818 1163 Size: 573 978 104 4154 Fragments arranged by size: 4154 978 573 104 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3259 4020 5048 1540 Size: 275 186 1258 761 1028 2301 Fragments arranged by size: 2301 1258 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3870 3955 5026 3870 Size: 85 1071 4653 Fragments arranged by size: 4653 1071 85 BsmI GAATG_Cn' Cuts at: 3320 3413 3320 Size: 93 5716 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2790 2961 3099 3128 3870 3955 5026 Size: 399 1000 171 138 29 742 85 1071 Cuts at: 5026 1391 Size: 2174 Fragments arranged by size: 2174 1071 1000 742 399 171 138 85 29 BspEI T'CCGG_A Cuts at: 2892 5472 2892 Size: 2580 3229 BspHI T'CATG_A Cuts at: 3698 5428 3698 Size: 1730 4079 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2184 2626 3097 3164 844 Size: 1340 442 471 67 3489 Fragments arranged by size: 3489 1340 471 442 67 BsrBI CCG'CTC Cuts at: 1394 1823 3073 3696 1394 Size: 429 1250 623 3507 Fragments arranged by size: 3507 1250 623 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2342 4304 4478 65 Size: 1183 1094 1962 174 1396 Fragments arranged by size: 1962 1396 1183 1094 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2354 2682 4453 4640 1700 Size: 110 544 328 1771 187 2869 Fragments arranged by size: 2869 1771 544 328 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5809 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5809 BssSI C'ACGA_G Cuts at: 3869 4881 3869 Size: 1012 4797 BstAPI GCAn_nnn'nTGC Cuts at: 794 2692 794 Size: 1898 3911 BstBI TT'CG_AA Cuts at: 4613 4613 Size: 5809 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5809 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2590 2011 Size: 579 5230 BstYI r'GATC_y Cuts at: 1972 2232 2463 2535 2697 3139 3506 3891 3908 Size: 260 231 72 162 442 367 385 17 Cuts at: 3908 5360 5446 5458 5804 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 442 385 367 346 260 231 162 86 72 17 12 BtgI C'CryG_G Cuts at: 513 1065 2302 513 Size: 552 1237 4020 Fragments arranged by size: 4020 1237 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5070 Fragments arranged by size: 5070 683 56 BtsI GCAGTG_nn' Cuts at: 3325 4119 4139 3325 Size: 794 20 4995 Fragments arranged by size: 4995 794 20 ClaI AT'CG_AT Cuts at: 1554 3021 1554 Size: 1467 4342 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5774 DraI TTT'AAA Cuts at: 3113 3113 Size: 5809 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2405 4816 809 Size: 507 1089 2411 1802 Fragments arranged by size: 2411 1802 1089 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2157 3052 3256 Size: 54 1425 50 275 18 327 895 204 Cuts at: 3256 4145 5774 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 895 889 327 275 204 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3256 1537 Size: 275 1444 4090 Fragments arranged by size: 4090 1444 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3739 1353 Size: 2386 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2288 2729 4396 4770 4916 387 Size: 1064 138 699 441 1667 374 146 1280 Fragments arranged by size: 1667 1280 1064 699 441 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2417 2477 2498 2507 2585 2639 Size: 393 410 375 60 21 9 78 54 Cuts at: 2639 3872 4469 5256 5524 1239 Size: 1233 597 787 268 1524 Fragments arranged by size: 1524 1233 787 597 410 393 375 268 78 60 54 21 9 EcoNI CCTnn'n_nnAGG Cuts at: 2378 3104 2378 Size: 726 5083 EcoRI G'AATT_C Cuts at: 3118 3496 3118 Size: 378 5431 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2434 2434 Size: 5809 FspI TGC'GCA Cuts at: 4315 4315 Size: 5809 HaeII r_GCGC'y Cuts at: 1678 2374 2564 2945 4956 1678 Size: 696 190 381 2011 2531 Fragments arranged by size: 2531 2011 696 381 190 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3041 3073 4450 4482 4524 Size: 32 135 32 1469 32 1377 32 42 Cuts at: 4524 4556 1373 Size: 32 2626 Fragments arranged by size: 2626 1469 1377 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3153 3399 669 Size: 988 281 1215 246 3079 Fragments arranged by size: 3079 1215 988 281 246 HindIII A'AGCT_T Cuts at: 748 2742 748 Size: 1994 3815 HpaI GTT'AAC Cuts at: 3399 3399 Size: 5809 KpnI G_GTAC'C Cuts at: 3134 3134 Size: 5809 MfeI C'AATT_G Cuts at: 3408 3408 Size: 5809 MluI A'CGCG_T Cuts at: 5685 5685 Size: 5809 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2047 2250 2352 2841 2904 4923 5107 1305 Size: 742 203 102 489 63 2019 184 2007 Fragments arranged by size: 2019 2007 742 489 203 184 102 63 MscI TGG'CCA Cuts at: 10 64 1489 1832 2159 10 Size: 54 1425 343 327 3660 Fragments arranged by size: 3660 1425 343 327 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3767 4126 4285 518 Size: 648 843 1758 359 159 2042 Fragments arranged by size: 2042 1758 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2050 2671 3225 3902 5050 5295 1606 Size: 444 621 554 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 621 554 444 245 NaeI GCC'GGC Cuts at: 2356 2356 Size: 5809 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5257 NdeI CA'TA_TG Cuts at: 387 387 Size: 5809 NgoMIV G'CCGG_C Cuts at: 2354 2354 Size: 5809 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5809 NotI GC'GGCC_GC Cuts at: 3256 3256 Size: 5809 NspI r_CATG'y Cuts at: 1132 1229 2608 2914 3046 3167 4712 1132 Size: 97 1379 306 132 121 1545 2229 Fragments arranged by size: 2229 1545 1379 306 132 121 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5809 PciI A'CATG_T Cuts at: 4708 4708 Size: 5809 PflMI CCAn_nnn'nTGG Cuts at: 1108 2563 1108 Size: 1455 4354 PfoI T'CCnGG_A Cuts at: 2616 2700 2616 Size: 84 5725 PmeI GTTT'AAAC Cuts at: 3113 3113 Size: 5809 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3878 3910 5391 5423 3878 Size: 32 1481 32 4264 Fragments arranged by size: 4264 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5809 PsiI TTA'TAA Cuts at: 3379 3379 Size: 5809 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2243 2275 2243 Size: 32 5777 PstI C_TGCA'G Cuts at: 830 2181 3161 830 Size: 1351 980 3478 Fragments arranged by size: 3478 1351 980 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5809 PvuII CAG'CTG Cuts at: 2050 2671 2050 Size: 621 5188 SalI G'TCGA_C Cuts at: 1936 3151 1936 Size: 1215 4594 SbfI CC_TGCA'GG Cuts at: 3161 3161 Size: 5809 ScaI AGT'ACT Cuts at: 4057 5769 4057 Size: 1712 4097 SfcI C'TryA_G Cuts at: 826 1045 2177 3157 4292 4973 5164 826 Size: 219 1132 980 1135 681 191 1471 Fragments arranged by size: 1471 1135 1132 980 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5809 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5809 SmaI CCC'GGG Cuts at: 1872 2783 3136 1872 Size: 911 353 4545 Fragments arranged by size: 4545 911 353 SmlI C'TyrA_G Cuts at: 820 1017 1943 2723 3913 4814 5076 820 Size: 197 926 780 1190 901 262 1553 Fragments arranged by size: 1553 1190 926 901 780 262 197 SnaBI TAC'GTA Cuts at: 493 3600 493 Size: 3107 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5809 SphI G_CATG'C Cuts at: 1132 2608 2914 3046 3167 1132 Size: 1476 306 132 121 3774 Fragments arranged by size: 3774 1476 306 132 121 SspI AAT'ATT Cuts at: 5 52 3733 5 Size: 47 3681 2081 Fragments arranged by size: 3681 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5809 StyI C'CwwG_G Cuts at: 513 1065 2583 2797 3248 513 Size: 552 1518 214 451 3074 Fragments arranged by size: 3074 1518 552 451 214 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3836 3853 4006 4191 4636 325 Size: 1255 2256 17 153 185 445 1498 Fragments arranged by size: 2256 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4055 5767 96 Size: 274 80 33 51 1443 2078 1712 138 Fragments arranged by size: 2078 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5769 5801 5769 Size: 32 5777 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5809 XbaI T'CTAG_A Cuts at: 3145 3145 Size: 5809 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2371 2371 Size: 5809 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5809 XmaI C'CCGG_G Cuts at: 1870 2781 3134 1870 Size: 911 353 4545 Fragments arranged by size: 4545 911 353 XmnI GAAnn'nnTTC Cuts at: 2030 3938 2030 Size: 1908 3901 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2998 3617 276 Size: 53 83 186 936 1464 619 2468 Fragments arranged by size: 2468 1464 936 619 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI