(Circular) (Six-base) MAPSORT of: pFN21AB3466.seq Check: 5162 from: 1 to: 6436 pFN21AB3466 6436 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4246 278 Size: 53 83 186 936 2710 2468 Fragments arranged by size: 2710 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3779 1937 Size: 1842 4594 Acc65I G'GTAC_C Cuts at: 3757 3757 Size: 6436 AclI AA'CG_TT Cuts at: 1563 4563 4936 1563 Size: 3000 373 3063 Fragments arranged by size: 3063 3000 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2600 2605 3077 3213 3413 3590 4499 5883 Size: 968 5 472 136 200 177 909 1384 Cuts at: 5883 6151 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 968 909 472 268 200 177 136 5 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6239 AflIII A'CryG_T Cuts at: 1203 5335 6312 1203 Size: 4132 977 1327 Fragments arranged by size: 4132 1327 977 AgeI A'CCGG_T Cuts at: 5267 5267 Size: 6436 AhdI GACnn_n'nnGTC Cuts at: 5165 5165 Size: 6436 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6436 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6436 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2117 2149 2117 Size: 32 6404 AlwNI CAG_nnn'CTG Cuts at: 2305 3428 4179 5751 2305 Size: 1123 751 1572 2990 Fragments arranged by size: 2990 1572 1123 751 ApaLI G'TGCA_C Cuts at: 4493 5649 4493 Size: 1156 5280 ApoI r'AATT_y Cuts at: 1493 2765 3722 3745 3971 4123 6232 1493 Size: 1272 957 23 226 152 2109 1697 Fragments arranged by size: 2109 1697 1272 957 226 152 23 AseI AT'TA_AT Cuts at: 160 1032 4990 160 Size: 872 3958 1606 Fragments arranged by size: 3958 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6436 AvaI C'yCGr_G Cuts at: 1870 1943 2931 3225 3761 1870 Size: 73 988 294 536 4545 Fragments arranged by size: 4545 988 536 294 73 BamHI G'GATC_C Cuts at: 2517 3766 4133 2517 Size: 1249 367 4820 Fragments arranged by size: 4820 1249 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3757 5212 618 Size: 325 217 627 1970 1455 1842 Fragments arranged by size: 1970 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3121 3755 1391 Size: 1730 634 4072 Fragments arranged by size: 4072 1730 634 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2800 3372 3383 3401 3623 928 Size: 971 901 572 11 18 222 3741 Fragments arranged by size: 3741 971 901 572 222 18 11 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2005 2021 2039 4627 4661 1903 Size: 34 50 18 16 18 2588 34 3678 Fragments arranged by size: 3678 2588 50 34 34 18 18 16 BciVI GTATCCnnnnn_n' Cuts at: 4328 5538 4328 Size: 1210 5226 BclI T'GATC_A Cuts at: 1548 1548 Size: 6436 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3206 5047 136 Size: 107 122 71 631 605 1534 1841 1525 Fragments arranged by size: 1841 1534 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2593 6431 2593 Size: 3838 2598 BlpI GC'TnA_GC Cuts at: 3087 3387 3853 3087 Size: 300 466 5670 Fragments arranged by size: 5670 466 300 Bme1580I G_kGCm'C Cuts at: 1790 3151 4497 5653 1790 Size: 1361 1346 1156 2573 Fragments arranged by size: 2573 1361 1346 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6436 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3665 4167 5125 Size: 416 53 83 399 24 2248 502 958 Cuts at: 5125 442 Size: 1753 Fragments arranged by size: 2248 1753 958 502 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6436 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3429 3429 Size: 6436 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2072 2618 2749 3317 5096 1239 Size: 833 546 131 568 1779 2579 Fragments arranged by size: 2579 1779 833 568 546 131 Bpu10I CC'TnA_GC Cuts at: 2552 2552 Size: 6436 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2140 2574 3149 4561 5426 5724 2140 Size: 434 575 1412 865 298 2852 Fragments arranged by size: 2852 1412 865 575 434 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2357 2681 5099 882 Size: 617 858 324 2418 2219 Fragments arranged by size: 2418 2219 858 617 324 BsaAI yAC'GTr Cuts at: 493 1204 4227 493 Size: 711 3023 2702 Fragments arranged by size: 3023 2702 711 BsaBI GATnn'nnATC Cuts at: 6430 6430 Size: 6436 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4243 4625 5260 275 Size: 53 83 186 936 2710 382 635 1451 Fragments arranged by size: 2710 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4869 5267 5541 5688 6099 4869 Size: 398 274 147 411 5206 Fragments arranged by size: 5206 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6211 Fragments arranged by size: 6211 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2525 2951 3053 3533 1186 Size: 1339 426 102 480 4089 Fragments arranged by size: 4089 1339 480 426 102 BseYI C'CCAG_C Cuts at: 2656 3291 5639 6135 2656 Size: 635 2348 496 2957 Fragments arranged by size: 2957 2348 635 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2930 3578 1163 Size: 573 1194 648 4021 Fragments arranged by size: 4021 1194 648 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3886 4647 5675 1540 Size: 275 186 1885 761 1028 2301 Fragments arranged by size: 2301 1885 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4497 4582 5653 4497 Size: 85 1071 5280 Fragments arranged by size: 5280 1071 85 BsmI GAATG_Cn' Cuts at: 2331 3947 4040 2331 Size: 1616 93 4727 Fragments arranged by size: 4727 1616 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3121 3151 3755 4497 4582 5653 1391 Size: 399 1331 30 604 742 85 1071 2174 Fragments arranged by size: 2174 1331 1071 742 604 399 85 30 BspEI T'CCGG_A Cuts at: 6099 6099 Size: 6436 BspHI T'CATG_A Cuts at: 4325 6055 4325 Size: 1730 4706 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3791 844 Size: 2947 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2515 4323 1394 Size: 429 692 1808 3507 Fragments arranged by size: 3507 1808 692 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4931 5105 65 Size: 1183 3683 174 1396 Fragments arranged by size: 3683 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5080 5267 1700 Size: 110 3270 187 2869 Fragments arranged by size: 3270 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6436 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6436 BssSI C'ACGA_G Cuts at: 4496 5508 4496 Size: 1012 5424 BstAPI GCAn_nnn'nTGC Cuts at: 794 3428 794 Size: 2634 3802 BstBI TT'CG_AA Cuts at: 5240 5240 Size: 6436 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6436 BstYI r'GATC_y Cuts at: 1972 2517 2593 2841 3687 3766 4133 4518 4535 Size: 545 76 248 846 79 367 385 17 Cuts at: 4535 5987 6073 6085 6431 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 846 545 385 367 346 248 86 79 76 17 12 Bsu36I CC'TnA_GG Cuts at: 2262 2262 Size: 6436 BtgI C'CryG_G Cuts at: 513 1065 2037 2542 513 Size: 552 972 505 4407 Fragments arranged by size: 4407 972 552 505 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5697 Fragments arranged by size: 5697 683 56 BtsI GCAGTG_nn' Cuts at: 3520 3952 4746 4766 3520 Size: 432 794 20 5190 Fragments arranged by size: 5190 794 432 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6436 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6401 DraI TTT'AAA Cuts at: 3740 3740 Size: 6436 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5443 809 Size: 507 4127 1802 Fragments arranged by size: 4127 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2445 3171 3501 Size: 54 1425 50 275 18 615 726 330 Cuts at: 3501 3591 3883 4772 6401 8 Size: 90 292 889 1629 43 Fragments arranged by size: 1629 1425 889 726 615 330 292 275 90 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3883 1537 Size: 275 2071 4090 Fragments arranged by size: 4090 2071 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3189 4366 1353 Size: 1836 1177 3423 Fragments arranged by size: 3423 1836 1177 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5023 5397 5543 387 Size: 1064 138 3434 374 146 1280 Fragments arranged by size: 3434 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 2600 2605 2618 2749 3077 3213 Size: 393 440 528 5 13 131 328 136 Cuts at: 3213 3317 3413 3590 4499 5096 5883 6151 1239 Size: 104 96 177 909 597 787 268 1524 Fragments arranged by size: 1524 909 787 597 528 440 393 328 268 177 136 131 104 96 13 5 EcoO109I rG'GnC_Cy Cuts at: 3574 3574 Size: 6436 EcoRI G'AATT_C Cuts at: 3745 4123 3745 Size: 378 6058 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2737 2860 3226 2737 Size: 123 366 5947 Fragments arranged by size: 5947 366 123 FspI TGC'GCA Cuts at: 4942 4942 Size: 6436 HaeII r_GCGC'y Cuts at: 1678 2305 5583 1678 Size: 627 3278 2531 Fragments arranged by size: 3278 2531 627 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2255 2287 5077 5109 5151 Size: 32 135 32 683 32 2790 32 42 Cuts at: 5151 5183 1373 Size: 32 2626 Fragments arranged by size: 2790 2626 683 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3780 4026 669 Size: 988 281 1842 246 3079 Fragments arranged by size: 3079 1842 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6436 HpaI GTT'AAC Cuts at: 4026 4026 Size: 6436 KpnI G_GTAC'C Cuts at: 3761 3761 Size: 6436 MfeI C'AATT_G Cuts at: 4035 4035 Size: 6436 MluI A'CGCG_T Cuts at: 6312 6312 Size: 6436 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5550 5734 1305 Size: 4245 184 2007 Fragments arranged by size: 4245 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3173 3503 3593 10 Size: 54 1425 343 1341 330 90 2853 Fragments arranged by size: 2853 1425 1341 343 330 90 54 MslI CAynn'nnrTG Cuts at: 518 1166 2906 4394 4753 4912 518 Size: 648 1740 1488 359 159 2042 Fragments arranged by size: 2042 1740 1488 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2369 3253 3295 3852 4529 5677 5922 1606 Size: 763 884 42 557 677 1148 245 2120 Fragments arranged by size: 2120 1148 884 763 677 557 245 42 NcoI C'CATG_G Cuts at: 513 1065 2037 513 Size: 552 972 4912 Fragments arranged by size: 4912 972 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6436 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6436 NotI GC'GGCC_GC Cuts at: 3883 3883 Size: 6436 NspI r_CATG'y Cuts at: 1132 1229 2551 2881 3652 3794 5339 1132 Size: 97 1322 330 771 142 1545 2229 Fragments arranged by size: 2229 1545 1322 771 330 142 97 PasI CC'CwG_GG Cuts at: 1348 2671 1348 Size: 1323 5113 PciI A'CATG_T Cuts at: 5335 5335 Size: 6436 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6436 PfoI T'CCnGG_A Cuts at: 2475 2568 2475 Size: 93 6343 PmeI GTTT'AAAC Cuts at: 3740 3740 Size: 6436 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4505 4537 6018 6050 4505 Size: 32 1481 32 4891 Fragments arranged by size: 4891 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6436 PsiI TTA'TAA Cuts at: 4006 4006 Size: 6436 PstI C_TGCA'G Cuts at: 830 2148 3219 3788 830 Size: 1318 1071 569 3478 Fragments arranged by size: 3478 1318 1071 569 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6436 PvuII CAG'CTG Cuts at: 3253 3295 3253 Size: 42 6394 SalI G'TCGA_C Cuts at: 1936 3778 1936 Size: 1842 4594 SbfI CC_TGCA'GG Cuts at: 3788 3788 Size: 6436 ScaI AGT'ACT Cuts at: 4684 6396 4684 Size: 1712 4724 SexAI A'CCwGG_T Cuts at: 2097 2097 Size: 6436 SfcI C'TryA_G Cuts at: 826 1045 2144 3215 3784 4919 5600 5791 826 Size: 219 1099 1071 569 1135 681 191 1471 Fragments arranged by size: 1471 1135 1099 1071 681 569 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3206 3206 Size: 6436 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6436 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6436 SmaI CCC'GGG Cuts at: 1872 2933 3227 3763 1872 Size: 1061 294 536 4545 Fragments arranged by size: 4545 1061 536 294 SmlI C'TyrA_G Cuts at: 820 1017 1943 2119 2589 3128 4540 5441 5703 Size: 197 926 176 470 539 1412 901 262 Cuts at: 5703 820 Size: 1553 Fragments arranged by size: 1553 1412 926 901 539 470 262 197 176 SnaBI TAC'GTA Cuts at: 493 4227 493 Size: 3734 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6436 SphI G_CATG'C Cuts at: 1132 2551 3794 1132 Size: 1419 1243 3774 Fragments arranged by size: 3774 1419 1243 SspI AAT'ATT Cuts at: 5 52 4360 5 Size: 47 4308 2081 Fragments arranged by size: 4308 2081 47 StuI AGG'CCT Cuts at: 1517 3209 3221 1517 Size: 1692 12 4732 Fragments arranged by size: 4732 1692 12 StyI C'CwwG_G Cuts at: 513 1065 2037 3174 3201 3258 3504 3875 513 Size: 552 972 1137 27 57 246 371 3074 Fragments arranged by size: 3074 1137 972 552 371 246 57 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4463 4480 4633 4818 5263 325 Size: 1255 2883 17 153 185 445 1498 Fragments arranged by size: 2883 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3407 4682 6394 Size: 274 80 33 51 1443 1430 1275 1712 Cuts at: 6394 96 Size: 138 Fragments arranged by size: 1712 1443 1430 1275 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6396 6428 6396 Size: 32 6404 Tth111I GACn'n_nGTC Cuts at: 1316 2804 1316 Size: 1488 4948 XbaI T'CTAG_A Cuts at: 3772 3772 Size: 6436 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2402 2402 Size: 6436 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6436 XmaI C'CCGG_G Cuts at: 1870 2931 3225 3761 1870 Size: 1061 294 536 4545 Fragments arranged by size: 4545 1061 536 294 XmnI GAAnn'nnTTC Cuts at: 2283 2618 4565 2283 Size: 335 1947 4154 Fragments arranged by size: 4154 1947 335 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4244 276 Size: 53 83 186 936 2710 2468 Fragments arranged by size: 2710 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfoI SrfI SwaI