(Circular) (Six-base) MAPSORT of: pFN21AB3086.seq Check: 6270 from: 1 to: 6445 pFN21AB3086 6445 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2855 2855 Size: 6445 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4255 278 Size: 53 83 186 936 2719 2468 Fragments arranged by size: 2719 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3788 1937 Size: 1851 4594 Acc65I G'GTAC_C Cuts at: 3766 3766 Size: 6445 AclI AA'CG_TT Cuts at: 1563 3675 4572 4945 1563 Size: 2112 897 373 3063 Fragments arranged by size: 3063 2112 897 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2267 2678 2762 3114 3422 3710 4508 5892 Size: 635 411 84 352 308 288 798 1384 Cuts at: 5892 6160 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 798 635 411 352 308 288 268 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6248 AflIII A'CryG_T Cuts at: 1203 5344 6321 1203 Size: 4141 977 1327 Fragments arranged by size: 4141 1327 977 AgeI A'CCGG_T Cuts at: 5276 5276 Size: 6445 AhdI GACnn_n'nnGTC Cuts at: 5174 5174 Size: 6445 AleI CACnn'nnGTG Cuts at: 1166 2456 1166 Size: 1290 5155 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6445 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2291 2323 3665 3697 2291 Size: 32 1342 32 5039 Fragments arranged by size: 5039 1342 32 32 AlwNI CAG_nnn'CTG Cuts at: 2137 2701 2806 3421 4188 5760 2137 Size: 564 105 615 767 1572 2822 Fragments arranged by size: 2822 1572 767 615 564 105 ApaI G_GGCC'C Cuts at: 2213 3368 2213 Size: 1155 5290 ApaLI G'TGCA_C Cuts at: 3740 4502 5658 3740 Size: 762 1156 4527 Fragments arranged by size: 4527 1156 762 ApoI r'AATT_y Cuts at: 1493 2444 2632 3754 3980 4132 6241 1493 Size: 951 188 1122 226 152 2109 1697 Fragments arranged by size: 2109 1697 1122 951 226 188 152 AseI AT'TA_AT Cuts at: 160 1032 4999 160 Size: 872 3967 1606 Fragments arranged by size: 3967 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6445 AvaI C'yCGr_G Cuts at: 1870 1943 2065 3668 3770 1870 Size: 73 122 1603 102 4545 Fragments arranged by size: 4545 1603 122 102 73 BamHI G'GATC_C Cuts at: 3775 4142 3775 Size: 367 6078 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2158 3766 5221 618 Size: 325 217 627 371 1608 1455 1842 Fragments arranged by size: 1842 1608 1455 627 371 325 217 BanII G_rGCy'C Cuts at: 1391 2213 2304 3368 3764 1391 Size: 822 91 1064 396 4072 Fragments arranged by size: 4072 1064 822 396 91 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5474 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4636 4670 1903 Size: 34 2699 34 3678 Fragments arranged by size: 3678 2699 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4337 5547 4337 Size: 1210 5235 BclI T'GATC_A Cuts at: 1548 2721 2734 1548 Size: 1173 13 5259 Fragments arranged by size: 5259 1173 13 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5056 136 Size: 107 122 71 631 605 3384 1525 Fragments arranged by size: 3384 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3077 3097 6440 3077 Size: 20 3343 3082 Fragments arranged by size: 3343 3082 20 BlpI GC'TnA_GC Cuts at: 2051 3862 2051 Size: 1811 4634 Bme1580I G_kGCm'C Cuts at: 1790 2213 3055 3368 3744 4506 5662 1790 Size: 423 842 313 376 762 1156 2573 Fragments arranged by size: 2573 1156 842 762 423 376 313 BmgBI CAC'GTC Cuts at: 1318 2612 1318 Size: 1294 5151 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4176 5134 442 Size: 416 53 83 399 24 2759 958 1753 Fragments arranged by size: 2759 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6445 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2911 5105 1239 Size: 1672 2194 2579 Fragments arranged by size: 2579 2194 1672 Bpu10I CC'TnA_GC Cuts at: 3369 3369 Size: 6445 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2135 2209 2288 4570 5435 5733 2135 Size: 74 79 2282 865 298 2847 Fragments arranged by size: 2847 2282 865 298 79 74 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2975 5108 882 Size: 617 1476 2133 2219 Fragments arranged by size: 2219 2133 1476 617 BsaAI yAC'GTr Cuts at: 493 1204 4236 493 Size: 711 3032 2702 Fragments arranged by size: 3032 2702 711 BsaBI GATnn'nnATC Cuts at: 6439 6439 Size: 6445 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2100 4252 4634 5269 Size: 53 83 186 936 567 2152 382 635 Cuts at: 5269 275 Size: 1451 Fragments arranged by size: 2152 1451 936 635 567 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4878 5276 5550 5697 6108 4878 Size: 398 274 147 411 5215 Fragments arranged by size: 5215 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2291 2321 1178 Size: 30 165 30 888 30 5302 Fragments arranged by size: 5302 888 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2616 1186 Size: 1430 5015 BseYI C'CCAG_C Cuts at: 2544 5648 6144 2544 Size: 3104 496 2845 Fragments arranged by size: 3104 2845 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5872 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3895 4656 5684 1540 Size: 275 186 1894 761 1028 2301 Fragments arranged by size: 2301 1894 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2304 3744 4506 4591 5662 2304 Size: 1440 762 85 1071 3087 Fragments arranged by size: 3087 1440 1071 762 85 BsmI GAATG_Cn' Cuts at: 2329 2772 3019 3956 4049 2329 Size: 443 247 937 93 4725 Fragments arranged by size: 4725 937 443 247 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2213 2304 3055 3368 3744 3764 4506 Size: 399 423 91 751 313 376 20 742 Cuts at: 4506 4591 5662 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 751 742 423 399 376 313 91 85 20 BspEI T'CCGG_A Cuts at: 6108 6108 Size: 6445 BspHI T'CATG_A Cuts at: 4334 6064 4334 Size: 1730 4715 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2855 3800 844 Size: 2011 945 3489 Fragments arranged by size: 3489 2011 945 BsrBI CCG'CTC Cuts at: 1394 1823 2121 4332 1394 Size: 429 298 2211 3507 Fragments arranged by size: 3507 2211 429 298 BsrDI GCAATG_nn' Cuts at: 65 1248 2845 4940 5114 65 Size: 1183 1597 2095 174 1396 Fragments arranged by size: 2095 1597 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5089 5276 1700 Size: 110 3279 187 2869 Fragments arranged by size: 3279 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2934 96 Size: 2838 3607 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6445 BssSI C'ACGA_G Cuts at: 3287 4505 5517 3287 Size: 1218 1012 4215 Fragments arranged by size: 4215 1218 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2028 794 Size: 1234 5211 BstBI TT'CG_AA Cuts at: 5249 5249 Size: 6445 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6445 BstXI CCAn_nnnn'nTGG Cuts at: 3718 3718 Size: 6445 BstYI r'GATC_y Cuts at: 1972 2685 3077 3097 3775 4142 4527 4544 5996 Size: 713 392 20 678 367 385 17 1452 Cuts at: 5996 6082 6094 6440 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 713 678 392 385 367 346 86 20 17 12 Bsu36I CC'TnA_GG Cuts at: 2205 2205 Size: 6445 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4954 Fragments arranged by size: 4954 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2057 487 Size: 683 56 831 4875 Fragments arranged by size: 4875 831 683 56 BtsI GCAGTG_nn' Cuts at: 2669 3961 4755 4775 2669 Size: 1292 794 20 4339 Fragments arranged by size: 4339 1292 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6445 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3019 3054 529 Size: 35 2455 35 3920 Fragments arranged by size: 3920 2455 35 35 DraI TTT'AAA Cuts at: 3749 3749 Size: 6445 DraIII CAC_nnn'GTG Cuts at: 2028 2028 Size: 6445 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5452 809 Size: 507 4136 1802 Fragments arranged by size: 4136 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2761 3708 Size: 54 1425 50 275 18 177 754 947 Cuts at: 3708 3892 4781 6410 8 Size: 184 889 1629 43 Fragments arranged by size: 1629 1425 947 889 754 275 184 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3892 1537 Size: 275 2080 4090 Fragments arranged by size: 4090 2080 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4375 1353 Size: 3022 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3266 5032 5406 5552 387 Size: 1064 138 1677 1766 374 146 1280 Fragments arranged by size: 1766 1677 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2302 2302 Size: 6445 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2267 2678 2762 2911 3114 3422 3710 Size: 393 635 411 84 149 203 308 288 Cuts at: 3710 4508 5105 5892 6160 1239 Size: 798 597 787 268 1524 Fragments arranged by size: 1524 798 787 635 597 411 393 308 288 268 203 149 84 EcoNI CCTnn'n_nnAGG Cuts at: 3470 3470 Size: 6445 EcoO109I rG'GnC_Cy Cuts at: 2209 3364 3365 2209 Size: 1155 1 5289 Fragments arranged by size: 5289 1155 1 EcoRI G'AATT_C Cuts at: 3754 4132 3754 Size: 378 6067 FspI TGC'GCA Cuts at: 2021 4951 2021 Size: 2930 3515 FspAI rTGC'GCAy Cuts at: 2021 2021 Size: 6445 HaeII r_GCGC'y Cuts at: 1678 5592 1678 Size: 3914 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3270 3302 5086 5118 5160 Size: 32 135 32 1698 32 1784 32 42 Cuts at: 5160 5192 1373 Size: 32 2626 Fragments arranged by size: 2626 1784 1698 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3789 4035 669 Size: 988 281 1851 246 3079 Fragments arranged by size: 3079 1851 988 281 246 HindIII A'AGCT_T Cuts at: 748 2424 2642 3692 748 Size: 1676 218 1050 3501 Fragments arranged by size: 3501 1676 1050 218 HpaI GTT'AAC Cuts at: 4035 4035 Size: 6445 KpnI G_GTAC'C Cuts at: 3770 3770 Size: 6445 MfeI C'AATT_G Cuts at: 4044 4044 Size: 6445 MluI A'CGCG_T Cuts at: 6321 6321 Size: 6445 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5559 5743 1305 Size: 4254 184 2007 Fragments arranged by size: 4254 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3710 10 Size: 54 1425 343 1878 2745 Fragments arranged by size: 2745 1878 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2456 2777 3434 3704 3716 4403 4762 Size: 648 1290 321 657 270 12 687 359 Cuts at: 4762 4921 518 Size: 159 2042 Fragments arranged by size: 2042 1290 687 657 648 359 321 270 159 12 MspA1I CmG'CkG Cuts at: 1606 2278 3688 3861 4538 5686 5931 1606 Size: 672 1410 173 677 1148 245 2120 Fragments arranged by size: 2120 1410 1148 677 672 245 173 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4954 Fragments arranged by size: 4954 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6445 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6445 NotI GC'GGCC_GC Cuts at: 3892 3892 Size: 6445 NspI r_CATG'y Cuts at: 1132 1229 2220 2935 3803 5348 1132 Size: 97 991 715 868 1545 2229 Fragments arranged by size: 2229 1545 991 868 715 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6445 PciI A'CATG_T Cuts at: 5344 5344 Size: 6445 PflMI CCAn_nnn'nTGG Cuts at: 1108 2781 1108 Size: 1673 4772 PmeI GTTT'AAAC Cuts at: 3749 3749 Size: 6445 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2291 2323 4514 4546 6027 6059 2291 Size: 32 2191 32 1481 32 2677 Fragments arranged by size: 2677 2191 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6445 PsiI TTA'TAA Cuts at: 4015 4015 Size: 6445 PspOMI G'GGCC_C Cuts at: 2209 3364 2209 Size: 1155 5290 PstI C_TGCA'G Cuts at: 830 2142 3236 3797 830 Size: 1312 1094 561 3478 Fragments arranged by size: 3478 1312 1094 561 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6445 PvuII CAG'CTG Cuts at: 2278 2278 Size: 6445 SacI G_AGCT'C Cuts at: 2304 2304 Size: 6445 SalI G'TCGA_C Cuts at: 1936 3787 1936 Size: 1851 4594 SbfI CC_TGCA'GG Cuts at: 3797 3797 Size: 6445 ScaI AGT'ACT Cuts at: 2888 4693 6405 2888 Size: 1805 1712 2928 Fragments arranged by size: 2928 1805 1712 SfcI C'TryA_G Cuts at: 826 1045 2138 3208 3232 3793 4928 5609 5800 Size: 219 1093 1070 24 561 1135 681 191 Cuts at: 5800 826 Size: 1471 Fragments arranged by size: 1471 1135 1093 1070 681 561 219 191 24 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6445 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6445 SmaI CCC'GGG Cuts at: 1872 2067 3772 1872 Size: 195 1705 4545 Fragments arranged by size: 4545 1705 195 SmlI C'TyrA_G Cuts at: 820 1017 1943 2150 2224 2303 4549 5450 5712 Size: 197 926 207 74 79 2246 901 262 Cuts at: 5712 820 Size: 1553 Fragments arranged by size: 2246 1553 926 901 262 207 197 79 74 SnaBI TAC'GTA Cuts at: 493 4236 493 Size: 3743 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6445 SphI G_CATG'C Cuts at: 1132 3803 1132 Size: 2671 3774 SspI AAT'ATT Cuts at: 5 52 4369 5 Size: 47 4317 2081 Fragments arranged by size: 4317 2081 47 StuI AGG'CCT Cuts at: 1517 2345 2562 1517 Size: 828 217 5400 Fragments arranged by size: 5400 828 217 StyI C'CwwG_G Cuts at: 513 1065 2004 2577 3884 513 Size: 552 939 573 1307 3074 Fragments arranged by size: 3074 1307 939 573 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2146 2429 4472 4489 4642 4827 5272 Size: 1255 566 283 2043 17 153 185 445 Cuts at: 5272 325 Size: 1498 Fragments arranged by size: 2043 1498 1255 566 445 283 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2886 2934 4691 Size: 274 80 33 51 1443 909 48 1757 Cuts at: 4691 6403 96 Size: 1712 138 Fragments arranged by size: 1757 1712 1443 909 274 138 80 51 48 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2703 2735 6405 6437 2703 Size: 32 3670 32 2711 Fragments arranged by size: 3670 2711 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2501 1316 Size: 1185 5260 XbaI T'CTAG_A Cuts at: 3781 3781 Size: 6445 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6445 XmaI C'CCGG_G Cuts at: 1870 2065 3770 1870 Size: 195 1705 4545 Fragments arranged by size: 4545 1705 195 XmnI GAAnn'nnTTC Cuts at: 2901 3177 3194 4574 2901 Size: 276 17 1380 4772 Fragments arranged by size: 4772 1380 276 17 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4253 276 Size: 53 83 186 936 2719 2468 Fragments arranged by size: 2719 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI