Restriction map for
pFN21AB9562
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaI BsiWI BsmBI BsmI Bsp1407I
BspDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I Bsu36I CelII
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI KspI MfeI
MluI MseI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SbfI ScaI
SfiI SfuI SnaBI SpeI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 21 enzymes) #
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AcsI 1150 AAATTC [GA]^AATT[CT]
ApoI 1150 AAATTC [GA]^AATT[CT]
BamHI 794 GGATCC G^GATCC
BbeI 1739 GGCGCC GGCGC^C
BglII 2129 AGATCT A^GATCT
BspHI 2090 TCATGA T^CATGA
BspLU11I 2279 ACATGT A^CATGT
BsrDI 444 CATTGC GCAATG(2/0)
BstEII 1500 GGTGACC G^GT[ACGT]ACC
BstPI 1500 GGTGACC G^GT[ACGT]ACC
EcoO65I 1500 GGTGACC G^GT[ACGT]ACC
HaeII 1739 GGCGCC [GA]GCGC^[CT]
KasI 1739 GGCGCC G^GCGCC
NarI 1739 GGCGCC GG^CGCC
RcaI 2090 TCATGA T^CATGA
SalI 6 GTCGAC G^TCGAC
SfoI 1739 GGCGCC GGC^GCC
SgrAI 1741 CGCCGGCG C[GA]^CCGG[CT]G
SphI 408 GCATGC GCATG^C
StuI 1532 AGGCCT AGG^CCT
Tsp509I 1151 AATT ^AATT
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# TWO CUT ENZYMES ( 31 enzymes) #
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AatII 517 GACGTC GACGT^C
1180 GACGTC
AccI 6 GTCGAC GT^[AC][GT]AC
1651 GTCTAC
AccII 307 CGCG CG^CG
1336 CGCG
AccIII 29 TCCGGA T^CCGGA
672 TCCGGA
AflIII 1469 ACGTGT A^C[GA][CT]GT
2279 ACATGT
AspHI 495 GAGCAC G[AT]GC[AT]^C
859 GTGCTC
BfaI 1114 CTAG C^TAG
1186 CTAG
BsaBI 943 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2130 GATCTGCATC
BseAI 29 TCCGGA T^CCGGA
672 TCCGGA
BsiEI 8 CGACCG CG[GA][CT]^CG
2002 CGACCG
BsiHKAI 495 GAGCAC G[AT]GC[AT]^C
859 GTGCTC
BsmAI 1239 GTCTC GTCTC(1/5)
1764 GTCTC
BspEI 29 TCCGGA T^CCGGA
672 TCCGGA
BsrBI 1287 GAGCGG CCGCTC(-3/-3)
1294 GAGCGG
BstUI 307 CGCG CG^CG
1336 CGCG
CfoI 1671 GCGC GCG^C
1740 GCGC
DraIII 201 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
762 CACCGTGTG
HgaI 60 GACGC GACGC(5/10)
2288 GCGTC
HhaI 1671 GCGC GCG^C
1740 GCGC
HinP1I 1671 GCGC G^CGC
1740 GCGC
HincII 6 GTCGAC GT[CT]^[GA]AC
1465 GTCAAC
HindII 6 GTCGAC GT[CT]^[GA]AC
1465 GTCAAC
MaeI 1114 CTAG C^TAG
1186 CTAG
MamI 943 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2130 GATCTGCATC
MroI 29 TCCGGA T^CCGGA
672 TCCGGA
MvnI 307 CGCG CG^CG
1336 CGCG
PvuII 534 CAGCTG CAG^CTG
1687 CAGCTG
SmaI 367 CCCGGG CCC^GGG
1226 CCCGGG
XcmI 899 CCAGCCCCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2038 CCAACGCCAGAGTGG
XmaCI 367 CCCGGG C^CCGGG
1226 CCCGGG
XmaI 367 CCCGGG C^CCGGG
1226 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaHI BsaJI BsaWI
BseRI BsgI BsiYI BslI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr10I Cfr13I DdeI DpnII
DraII DrdI DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII Hin1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI SapI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI StyI
TaqI TseI Tsp45I TspRI TthHB8I
Van91I XhoII