(Circular) (Six-base) MAPSORT of: pFN21AB9562.seq Check: 6014 from: 1 to: 7069 pFN21AB9562 7069 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2823 3537 4099 4114 2823 Size: 714 562 15 5778 Fragments arranged by size: 5778 714 562 15 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2526 3189 4879 278 Size: 53 83 186 936 990 663 1690 2468 Fragments arranged by size: 2468 1690 990 936 663 186 83 53 AccI GT'mk_AC Cuts at: 1937 2012 3657 4412 1937 Size: 75 1645 755 4594 Fragments arranged by size: 4594 1645 755 75 Acc65I G'GTAC_C Cuts at: 4390 4390 Size: 7069 AclI AA'CG_TT Cuts at: 1563 5196 5569 1563 Size: 3633 373 3063 Fragments arranged by size: 3633 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3194 5132 6516 6784 1632 Size: 1562 1938 1384 268 1917 Fragments arranged by size: 1938 1917 1562 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6872 AflIII A'CryG_T Cuts at: 1203 3474 4284 5968 6945 1203 Size: 2271 810 1684 977 1327 Fragments arranged by size: 2271 1684 1327 977 810 AgeI A'CCGG_T Cuts at: 5900 5900 Size: 7069 AhdI GACnn_n'nnGTC Cuts at: 5798 5798 Size: 7069 AleI CACnn'nnGTG Cuts at: 1166 2129 2312 2889 3267 3464 4199 4340 1166 Size: 963 183 577 378 197 735 141 3895 Fragments arranged by size: 3895 963 735 577 378 197 183 141 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2318 1184 Size: 1134 5935 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2975 3005 3007 3037 2975 Size: 30 2 30 7007 Fragments arranged by size: 7007 30 30 2 AlwNI CAG_nnn'CTG Cuts at: 2966 3115 4114 4812 6384 2966 Size: 149 999 698 1572 3651 Fragments arranged by size: 3651 1572 999 698 149 ApaLI G'TGCA_C Cuts at: 5126 6282 5126 Size: 1156 5913 ApoI r'AATT_y Cuts at: 1493 3155 4378 4604 4756 6865 1493 Size: 1662 1223 226 152 2109 1697 Fragments arranged by size: 2109 1697 1662 1223 226 152 AseI AT'TA_AT Cuts at: 160 1032 5623 160 Size: 872 4591 1606 Fragments arranged by size: 4591 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7069 AvaI C'yCGr_G Cuts at: 1870 1943 2372 3231 3246 4255 4394 1870 Size: 73 429 859 15 1009 139 4545 Fragments arranged by size: 4545 1009 859 429 139 73 15 BamHI G'GATC_C Cuts at: 2799 4399 4766 2799 Size: 1600 367 5102 Fragments arranged by size: 5102 1600 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2152 2921 2933 3423 3744 Size: 325 217 627 365 769 12 490 321 Cuts at: 3744 3791 3836 3911 4193 4390 5845 618 Size: 47 45 75 282 197 1455 1842 Fragments arranged by size: 1842 1455 769 627 490 365 325 321 282 217 197 75 47 45 12 BanII G_rGCy'C Cuts at: 1391 3765 3809 3939 3981 4043 4388 1391 Size: 2374 44 130 42 62 345 4072 Fragments arranged by size: 4072 2374 345 130 62 44 42 BbeI G_GCGC'C Cuts at: 3748 3748 Size: 7069 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3265 3355 3583 3601 928 Size: 971 1366 90 228 18 4396 Fragments arranged by size: 4396 1366 971 228 90 18 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5260 5294 1903 Size: 34 3323 34 3678 Fragments arranged by size: 3678 3323 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4961 6171 4961 Size: 1210 5859 BclI T'GATC_A Cuts at: 1548 1548 Size: 7069 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5680 136 Size: 107 122 71 631 605 4008 1525 Fragments arranged by size: 4008 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4134 7064 4134 Size: 2930 4139 BlpI GC'TnA_GC Cuts at: 4486 4486 Size: 7069 Bme1580I G_kGCm'C Cuts at: 1790 2373 3348 3426 3794 4221 4262 5130 6286 Size: 583 975 78 368 427 41 868 1156 Cuts at: 6286 1790 Size: 2573 Fragments arranged by size: 2573 1156 975 868 583 427 368 78 41 BmgBI CAC'GTC Cuts at: 1318 4342 1318 Size: 3024 4045 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3353 4800 5758 Size: 416 53 83 399 24 1936 1447 958 Cuts at: 5758 442 Size: 1753 Fragments arranged by size: 1936 1753 1447 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7069 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2209 3559 3811 2209 Size: 1350 252 5467 Fragments arranged by size: 5467 1350 252 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2365 2943 3050 3554 3623 3849 5729 1239 Size: 1126 578 107 504 69 226 1880 2579 Fragments arranged by size: 2579 1880 1126 578 504 226 107 69 Bpu10I CC'TnA_GC Cuts at: 3759 3933 3969 3759 Size: 174 36 6859 Fragments arranged by size: 6859 174 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3014 3473 3827 5194 6059 6357 3014 Size: 459 354 1367 865 298 3726 Fragments arranged by size: 3726 1367 865 459 354 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5732 882 Size: 617 4233 2219 Fragments arranged by size: 4233 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4860 493 Size: 711 3656 2702 Fragments arranged by size: 3656 2702 711 BsaBI GATnn'nnATC Cuts at: 2952 4139 7063 2952 Size: 1187 2924 2958 Fragments arranged by size: 2958 2924 1187 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2523 3186 3745 4293 Size: 53 83 186 936 990 663 559 548 Cuts at: 4293 4876 5258 5893 275 Size: 583 382 635 1451 Fragments arranged by size: 1451 990 936 663 635 583 559 548 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2034 2493 2613 2677 5502 5900 6174 6321 6732 Size: 459 120 64 2825 398 274 147 411 Cuts at: 6732 2034 Size: 2371 Fragments arranged by size: 2825 2371 459 411 398 274 147 120 64 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2513 2543 1178 Size: 30 165 30 1110 30 5704 Fragments arranged by size: 5704 1110 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2400 2512 2750 2864 3023 1186 Size: 1214 112 238 114 159 5232 Fragments arranged by size: 5232 1214 238 159 114 112 BseYI C'CCAG_C Cuts at: 2586 2671 3939 3980 6272 6768 2586 Size: 85 1268 41 2292 496 2887 Fragments arranged by size: 2887 2292 1268 496 85 41 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2153 2258 3509 1163 Size: 573 417 105 1251 4723 Fragments arranged by size: 4723 1251 573 417 105 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2016 4010 4519 5280 6308 1540 Size: 275 186 15 1994 509 761 1028 2301 Fragments arranged by size: 2301 1994 1028 761 509 275 186 15 BsiHKAI G_wGCw'C Cuts at: 2504 2868 5130 5215 6286 2504 Size: 364 2262 85 1071 3287 Fragments arranged by size: 3287 2262 1071 364 85 BsmI GAATG_Cn' Cuts at: 4580 4673 4580 Size: 93 6976 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2373 2504 2868 3348 3426 3765 3794 Size: 399 583 131 364 480 78 339 29 Cuts at: 3794 3809 3939 3981 4043 4221 4262 4388 5130 Size: 15 130 42 62 178 41 126 742 Cuts at: 5130 5215 6286 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 583 480 399 364 339 178 131 130 126 85 78 62 42 41 29 15 BspEI T'CCGG_A Cuts at: 2034 2677 6732 2034 Size: 643 4055 2371 Fragments arranged by size: 4055 2371 643 BspHI T'CATG_A Cuts at: 4095 4958 6688 4095 Size: 863 1730 4476 Fragments arranged by size: 4476 1730 863 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2466 2673 2823 3084 3393 3537 3711 4099 Size: 1622 207 150 261 309 144 174 388 Cuts at: 4099 4114 4424 844 Size: 15 310 3489 Fragments arranged by size: 3489 1622 388 310 309 261 207 174 150 144 15 BsrBI CCG'CTC Cuts at: 1394 1823 3294 3301 4956 1394 Size: 429 1471 7 1655 3507 Fragments arranged by size: 3507 1655 1471 429 7 BsrDI GCAATG_nn' Cuts at: 65 1248 2448 5564 5738 65 Size: 1183 1200 3116 174 1396 Fragments arranged by size: 3116 1396 1200 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2155 2298 3747 3776 5713 5900 1700 Size: 110 345 143 1449 29 1937 187 2869 Fragments arranged by size: 2869 1937 1449 345 187 143 110 29 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7069 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7069 BssSI C'ACGA_G Cuts at: 5129 6141 5129 Size: 1012 6057 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7069 BstBI TT'CG_AA Cuts at: 5873 5873 Size: 7069 BstEII G'GTnAC_C Cuts at: 1184 3505 1184 Size: 2321 4748 BstXI CCAn_nnnn'nTGG Cuts at: 2425 3638 3856 2425 Size: 1213 218 5638 Fragments arranged by size: 5638 1213 218 BstYI r'GATC_y Cuts at: 1972 2166 2799 3096 4134 4170 4399 4766 5151 Size: 194 633 297 1038 36 229 367 385 Cuts at: 5151 5168 6620 6706 6718 7064 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1038 633 385 367 346 297 229 194 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2073 2699 2721 2940 3348 3501 Size: 552 939 69 626 22 219 408 153 Cuts at: 3501 3708 3798 513 Size: 207 90 3784 Fragments arranged by size: 3784 939 626 552 408 219 207 153 90 69 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6330 Fragments arranged by size: 6330 683 56 BtsI GCAGTG_nn' Cuts at: 2905 4040 4260 4585 5379 5399 2905 Size: 1135 220 325 794 20 4575 Fragments arranged by size: 4575 1135 794 325 220 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7069 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7034 DraI TTT'AAA Cuts at: 4373 4373 Size: 7069 DraIII CAC_nnn'GTG Cuts at: 2211 2772 2211 Size: 561 6508 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3200 3359 3889 4162 4322 6076 809 Size: 507 1884 159 530 273 160 1754 1802 Fragments arranged by size: 1884 1802 1754 530 507 273 160 159 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2202 2427 2445 Size: 54 1425 50 275 18 372 225 18 Cuts at: 2445 3696 4516 5405 7034 8 Size: 1251 820 889 1629 43 Fragments arranged by size: 1629 1425 1251 889 820 372 275 225 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 4516 1537 Size: 275 2704 4090 Fragments arranged by size: 4090 2704 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2178 2394 2468 2753 2825 3131 3167 3170 Size: 825 216 74 285 72 306 36 3 Cuts at: 3170 3218 3449 3566 4999 1353 Size: 48 231 117 1433 3423 Fragments arranged by size: 3423 1433 825 306 285 231 216 117 74 72 48 36 3 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3766 5656 6030 6176 387 Size: 1064 138 2177 1890 374 146 1280 Fragments arranged by size: 2177 1890 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2365 2943 3050 3194 3554 3623 3849 Size: 393 733 578 107 144 360 69 226 Cuts at: 3849 5132 5729 6516 6784 1239 Size: 1283 597 787 268 1524 Fragments arranged by size: 1524 1283 787 733 597 578 393 360 268 226 144 107 69 EcoNI CCTnn'n_nnAGG Cuts at: 2846 3533 3602 2846 Size: 687 69 6313 Fragments arranged by size: 6313 687 69 EcoO109I rG'GnC_Cy Cuts at: 2335 2421 2963 3372 3733 4057 4150 2335 Size: 86 542 409 361 324 93 5254 Fragments arranged by size: 5254 542 409 361 324 93 86 EcoRI G'AATT_C Cuts at: 4378 4756 4378 Size: 378 6691 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2047 2047 Size: 7069 FspI TGC'GCA Cuts at: 5575 5575 Size: 7069 HaeII r_GCGC'y Cuts at: 1678 3748 6216 1678 Size: 2070 2468 2531 Fragments arranged by size: 2531 2468 2070 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2513 2545 3629 3661 3752 Size: 32 135 32 941 32 1084 32 91 Cuts at: 3752 3784 5710 5742 5784 5816 1373 Size: 32 1926 32 42 32 2626 Fragments arranged by size: 2626 1926 1084 941 135 91 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2013 3472 4413 4659 669 Size: 988 281 75 1459 941 246 3079 Fragments arranged by size: 3079 1459 988 941 281 246 75 HindIII A'AGCT_T Cuts at: 748 748 Size: 7069 HpaI GTT'AAC Cuts at: 4659 4659 Size: 7069 KasI G'GCGC_C Cuts at: 3744 3744 Size: 7069 KpnI G_GTAC'C Cuts at: 4394 4394 Size: 7069 MfeI C'AATT_G Cuts at: 4668 4668 Size: 7069 MluI A'CGCG_T Cuts at: 6945 6945 Size: 7069 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2220 3078 3672 6183 6367 1305 Size: 915 858 594 2511 184 2007 Fragments arranged by size: 2511 2007 915 858 594 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2204 2447 3698 10 Size: 54 1425 343 372 243 1251 3381 Fragments arranged by size: 3381 1425 1251 372 343 243 54 MslI CAynn'nnrTG Cuts at: 518 1166 2129 2312 2889 2939 3267 3395 3464 Size: 648 963 183 577 50 328 128 69 Cuts at: 3464 3797 4199 4265 4283 4340 5027 5386 5545 Size: 333 402 66 18 57 687 359 159 Cuts at: 5545 518 Size: 2042 Fragments arranged by size: 2042 963 687 648 577 402 359 333 328 183 159 128 69 66 57 50 18 MspA1I CmG'CkG Cuts at: 1606 2541 3420 3430 3694 3742 3818 4003 4084 Size: 935 879 10 264 48 76 185 81 Cuts at: 4084 4363 4485 5162 6310 6555 1606 Size: 279 122 677 1148 245 2120 Fragments arranged by size: 2120 1148 935 879 677 279 264 245 185 122 81 76 48 10 NaeI GCC'GGC Cuts at: 2157 2300 3749 2157 Size: 143 1449 5477 Fragments arranged by size: 5477 1449 143 NarI GG'CG_CC Cuts at: 3745 3745 Size: 7069 NcoI C'CATG_G Cuts at: 513 1065 2004 2699 2940 3348 3501 3798 513 Size: 552 939 695 241 408 153 297 3784 Fragments arranged by size: 3784 939 695 552 408 297 241 153 NdeI CA'TA_TG Cuts at: 387 387 Size: 7069 NgoMIV G'CCGG_C Cuts at: 2155 2298 3747 2155 Size: 143 1449 5477 Fragments arranged by size: 5477 1449 143 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7069 NotI GC'GGCC_GC Cuts at: 4516 4516 Size: 7069 NspI r_CATG'y Cuts at: 1132 1229 2417 2646 2738 4288 4427 5972 1132 Size: 97 1188 229 92 1550 139 1545 2229 Fragments arranged by size: 2229 1550 1545 1188 229 139 97 92 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7069 PciI A'CATG_T Cuts at: 4284 5968 4284 Size: 1684 5385 PflMI CCAn_nnn'nTGG Cuts at: 1108 2533 2700 3552 3586 3694 1108 Size: 1425 167 852 34 108 4483 Fragments arranged by size: 4483 1425 852 167 108 34 PfoI T'CCnGG_A Cuts at: 3600 4064 3600 Size: 464 6605 PmeI GTTT'AAAC Cuts at: 4373 4373 Size: 7069 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5138 5170 6651 6683 5138 Size: 32 1481 32 5524 Fragments arranged by size: 5524 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2335 3372 4057 2335 Size: 1037 685 5347 Fragments arranged by size: 5347 1037 685 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7069 PsiI TTA'TAA Cuts at: 4639 4639 Size: 7069 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3314 3346 3314 Size: 32 7037 PstI C_TGCA'G Cuts at: 830 2297 3741 3829 4089 4110 4188 4421 830 Size: 1467 1444 88 260 21 78 233 3478 Fragments arranged by size: 3478 1467 1444 260 233 88 78 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7069 PvuII CAG'CTG Cuts at: 2541 3694 2541 Size: 1153 5916 SalI G'TCGA_C Cuts at: 1936 2011 4411 1936 Size: 75 2400 4594 Fragments arranged by size: 4594 2400 75 SanDI GG'GwC_CC Cuts at: 3372 3372 Size: 7069 SapI GCTCTTCn'nnn_ Cuts at: 2468 2825 3170 2468 Size: 357 345 6367 Fragments arranged by size: 6367 357 345 SbfI CC_TGCA'GG Cuts at: 4421 4421 Size: 7069 ScaI AGT'ACT Cuts at: 5317 7029 5317 Size: 1712 5357 SexAI A'CCwGG_T Cuts at: 3551 3585 3651 3551 Size: 34 66 6969 Fragments arranged by size: 6969 66 34 SfcI C'TryA_G Cuts at: 826 1045 2293 3737 3825 4085 4106 4184 4417 Size: 219 1248 1444 88 260 21 78 233 Cuts at: 4417 5552 6233 6424 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1444 1248 1135 681 260 233 219 191 88 78 21 SfoI GGC'GCC Cuts at: 3746 3746 Size: 7069 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7069 SgrAI Cr'CCGG_yG Cuts at: 1700 3747 1700 Size: 2047 5022 SmaI CCC'GGG Cuts at: 1872 2374 3233 4396 1872 Size: 502 859 1163 4545 Fragments arranged by size: 4545 1163 859 502 SmlI C'TyrA_G Cuts at: 820 1017 1943 3029 3488 3842 5173 6074 6336 Size: 197 926 1086 459 354 1331 901 262 Cuts at: 6336 820 Size: 1553 Fragments arranged by size: 1553 1331 1086 926 901 459 354 262 197 SnaBI TAC'GTA Cuts at: 493 4860 493 Size: 4367 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7069 SphI G_CATG'C Cuts at: 1132 2417 4427 1132 Size: 1285 2010 3774 Fragments arranged by size: 3774 2010 1285 SrfI GCCC'GGGC Cuts at: 3233 3233 Size: 7069 SspI AAT'ATT Cuts at: 5 52 4993 5 Size: 47 4941 2081 Fragments arranged by size: 4941 2081 47 StuI AGG'CCT Cuts at: 1517 3539 1517 Size: 2022 5047 StyI C'CwwG_G Cuts at: 513 1065 2004 2699 2940 3348 3501 3798 4508 Size: 552 939 695 241 408 153 297 710 Cuts at: 4508 513 Size: 3074 Fragments arranged by size: 3074 939 710 695 552 408 297 241 153 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2030 3899 5096 5113 5266 5451 5896 Size: 1255 450 1869 1197 17 153 185 445 Cuts at: 5896 325 Size: 1498 Fragments arranged by size: 1869 1498 1255 1197 450 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5315 7027 96 Size: 274 80 33 51 1443 3338 1712 138 Fragments arranged by size: 3338 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7029 7061 7029 Size: 32 7037 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7069 XbaI T'CTAG_A Cuts at: 4405 4405 Size: 7069 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2911 4050 2911 Size: 1139 5930 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7069 XmaI C'CCGG_G Cuts at: 1870 2372 3231 4394 1870 Size: 502 859 1163 4545 Fragments arranged by size: 4545 1163 859 502 XmnI GAAnn'nnTTC Cuts at: 5198 5198 Size: 7069 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2524 3187 4877 276 Size: 53 83 186 936 990 663 1690 2468 Fragments arranged by size: 2468 1690 990 936 663 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII SfiI SwaI