Restriction map for
pFN21AB9562
(Linear) (Six-base) MAPSORT of: KIBB9562.seq Check: 8158 from: 1 to: 2364
KIBB9562.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 818 1532 2094 2109 2364
Size: 818 714 562 15 255
Fragments arranged by size:
818 714 562 255 15
AatII G_ACGT'C
Cuts at: 0 521 1184 2364
Size: 521 663 1180
Fragments arranged by size:
1180 663 521
AccI GT'mk_AC
Cuts at: 0 7 1652 2364
Size: 7 1645 712
Fragments arranged by size:
1645 712 7
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1189 2364
Size: 1189 1175
AflIII A'CryG_T
Cuts at: 0 1469 2279 2364
Size: 1469 810 85
Fragments arranged by size:
1469 810 85
AleI CACnn'nnGTG
Cuts at: 0 124 307 884 1262 1459 2194 2335 2364
Size: 124 183 577 378 197 735 141 29
Fragments arranged by size:
735 577 378 197 183 141 124 29
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 313 2364
Size: 313 2051
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 970 1000 1002 1032 2364
Size: 970 30 2 30 1332
Fragments arranged by size:
1332 970 30 30 2
AlwNI CAG_nnn'CTG
Cuts at: 0 961 1110 2109 2364
Size: 961 149 999 255
Fragments arranged by size:
999 961 255 149
ApoI r'AATT_y
Cuts at: 0 1150 2364
Size: 1150 1214
AvaI C'yCGr_G
Cuts at: 0 367 1226 1241 2250 2364
Size: 367 859 15 1009 114
Fragments arranged by size:
1009 859 367 114 15
BamHI G'GATC_C
Cuts at: 0 794 2364
Size: 794 1570
BanI G'GyrC_C
Cuts at: 0 147 916 928 1418 1739 1786 1831 1906
Size: 147 769 12 490 321 47 45 75
Cuts at: 1906 2188 2364
Size: 282 176
Fragments arranged by size:
769 490 321 282 176 147 75 47
45 12
BanII G_rGCy'C
Cuts at: 0 1760 1804 1934 1976 2038 2364
Size: 1760 44 130 42 62 326
Fragments arranged by size:
1760 326 130 62 44 42
BbeI G_GCGC'C
Cuts at: 0 1743 2364
Size: 1743 621
BbsI GAAGACnn'nnnn_
Cuts at: 0 1260 1350 1578 1596 2364
Size: 1260 90 228 18 768
Fragments arranged by size:
1260 768 228 90 18
BglII A'GATC_T
Cuts at: 0 2129 2364
Size: 2129 235
Bme1580I G_kGCm'C
Cuts at: 0 368 1343 1421 1789 2216 2257 2364
Size: 368 975 78 368 427 41 107
Fragments arranged by size:
975 427 368 368 107 78 41
BmgBI CAC'GTC
Cuts at: 0 2337 2364
Size: 2337 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 1348 2364
Size: 1348 1016
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 204 1554 1806 2364
Size: 204 1350 252 558
Fragments arranged by size:
1350 558 252 204
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 360 938 1045 1549 1618 1844 2364
Size: 360 578 107 504 69 226 520
Fragments arranged by size:
578 520 504 360 226 107 69
Bpu10I CC'TnA_GC
Cuts at: 0 1754 1928 1964 2364
Size: 1754 174 36 400
Fragments arranged by size:
1754 400 174 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1009 1468 1822 2364
Size: 1009 459 354 542
Fragments arranged by size:
1009 542 459 354
BsaBI GATnn'nnATC
Cuts at: 0 947 2134 2364
Size: 947 1187 230
Fragments arranged by size:
1187 947 230
BsaHI Gr'CG_yC
Cuts at: 0 518 1181 1740 2288 2364
Size: 518 663 559 548 76
Fragments arranged by size:
663 559 548 518 76
BsaWI w'CCGG_w
Cuts at: 0 29 488 608 672 2364
Size: 29 459 120 64 1692
Fragments arranged by size:
1692 459 120 64 29
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 508 538 2364
Size: 508 30 1826
Fragments arranged by size:
1826 508 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 395 507 745 859 1018 2364
Size: 395 112 238 114 159 1346
Fragments arranged by size:
1346 395 238 159 114 112
BseYI C'CCAG_C
Cuts at: 0 581 666 1934 1975 2364
Size: 581 85 1268 41 389
Fragments arranged by size:
1268 581 389 85 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 253 1504 2364
Size: 148 105 1251 860
Fragments arranged by size:
1251 860 148 105
BsiEI CG_ry'CG
Cuts at: 0 11 2005 2364
Size: 11 1994 359
Fragments arranged by size:
1994 359 11
BsiHKAI G_wGCw'C
Cuts at: 0 499 863 2364
Size: 499 364 1501
Fragments arranged by size:
1501 499 364
Bsp1286I G_dGCh'C
Cuts at: 0 368 499 863 1343 1421 1760 1789 1804
Size: 368 131 364 480 78 339 29 15
Cuts at: 1804 1934 1976 2038 2216 2257 2364
Size: 130 42 62 178 41 107
Fragments arranged by size:
480 368 364 339 178 131 130 107
78 62 42 41 29 15
BspEI T'CCGG_A
Cuts at: 0 29 672 2364
Size: 29 643 1692
Fragments arranged by size:
1692 643 29
BspHI T'CATG_A
Cuts at: 0 2090 2364
Size: 2090 274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 461 668 818 1079 1388 1532 1706 2094
Size: 461 207 150 261 309 144 174 388
Cuts at: 2094 2109 2364
Size: 15 255
Fragments arranged by size:
461 388 309 261 255 207 174 150
144 15
BsrBI CCG'CTC
Cuts at: 0 1289 1296 2364
Size: 1289 7 1068
Fragments arranged by size:
1289 1068 7
BsrDI GCAATG_nn'
Cuts at: 0 443 2364
Size: 443 1921
BsrFI r'CCGG_y
Cuts at: 0 150 293 1742 1771 2364
Size: 150 143 1449 29 593
Fragments arranged by size:
1449 593 150 143 29
BstEII G'GTnAC_C
Cuts at: 0 1500 2364
Size: 1500 864
BstXI CCAn_nnnn'nTGG
Cuts at: 0 420 1633 1851 2364
Size: 420 1213 218 513
Fragments arranged by size:
1213 513 420 218
BstYI r'GATC_y
Cuts at: 0 161 794 1091 2129 2165 2364
Size: 161 633 297 1038 36 199
Fragments arranged by size:
1038 633 297 199 161 36
BtgI C'CryG_G
Cuts at: 0 68 694 716 935 1343 1496 1703 1793
Size: 68 626 22 219 408 153 207 90
Cuts at: 1793 2364
Size: 571
Fragments arranged by size:
626 571 408 219 207 153 90 68
22
BtsI GCAGTG_nn'
Cuts at: 0 900 2035 2255 2364
Size: 900 1135 220 109
Fragments arranged by size:
1135 900 220 109
DraIII CAC_nnn'GTG
Cuts at: 0 206 767 2364
Size: 206 561 1597
Fragments arranged by size:
1597 561 206
DrdI GACnn_nn'nnGTC
Cuts at: 0 1195 1354 1884 2157 2317 2364
Size: 1195 159 530 273 160 47
Fragments arranged by size:
1195 530 273 160 159 47
EaeI y'GGCC_r
Cuts at: 0 197 422 440 1691 2364
Size: 197 225 18 1251 673
Fragments arranged by size:
1251 673 225 197 18
EarI CTCTTCn'nnn_
Cuts at: 0 173 389 463 748 820 1126 1162 1165
Size: 173 216 74 285 72 306 36 3
Cuts at: 1165 1213 1444 1561 2364
Size: 48 231 117 803
Fragments arranged by size:
803 306 285 231 216 173 117 74
72 48 36 3
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1761 2364
Size: 1761 603
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 360 938 1045 1189 1549 1618 1844 2364
Size: 360 578 107 144 360 69 226 520
Fragments arranged by size:
578 520 360 360 226 144 107 69
EcoNI CCTnn'n_nnAGG
Cuts at: 0 841 1528 1597 2364
Size: 841 687 69 767
Fragments arranged by size:
841 767 687 69
EcoO109I rG'GnC_Cy
Cuts at: 0 330 416 958 1367 1728 2052 2145 2364
Size: 330 86 542 409 361 324 93 219
Fragments arranged by size:
542 409 361 330 324 219 93 86
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 42 2364
Size: 42 2322
HaeII r_GCGC'y
Cuts at: 0 1743 2364
Size: 1743 621
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 508 540 1624 1656 1747 1779 2364
Size: 508 32 1084 32 91 32 585
Fragments arranged by size:
1084 585 508 91 32 32 32
HincII GTy'rAC
Cuts at: 0 8 1467 2364
Size: 8 1459 897
Fragments arranged by size:
1459 897 8
KasI G'GCGC_C
Cuts at: 0 1739 2364
Size: 1739 625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 1073 1667 2364
Size: 215 858 594 697
Fragments arranged by size:
858 697 594 215
MscI TGG'CCA
Cuts at: 0 199 442 1693 2364
Size: 199 243 1251 671
Fragments arranged by size:
1251 671 243 199
MslI CAynn'nnrTG
Cuts at: 0 124 307 884 934 1262 1390 1459 1792
Size: 124 183 577 50 328 128 69 333
Cuts at: 1792 2194 2260 2278 2335 2364
Size: 402 66 18 57 29
Fragments arranged by size:
577 402 333 328 183 128 124 69
66 57 50 29 18
MspA1I CmG'CkG
Cuts at: 0 536 1415 1425 1689 1737 1813 1998 2079
Size: 536 879 10 264 48 76 185 81
Cuts at: 2079 2358 2364
Size: 279 6
Fragments arranged by size:
879 536 279 264 185 81 76 48
10 6
NaeI GCC'GGC
Cuts at: 0 152 295 1744 2364
Size: 152 143 1449 620
Fragments arranged by size:
1449 620 152 143
NarI GG'CG_CC
Cuts at: 0 1740 2364
Size: 1740 624
NcoI C'CATG_G
Cuts at: 0 694 935 1343 1496 1793 2364
Size: 694 241 408 153 297 571
Fragments arranged by size:
694 571 408 297 241 153
NgoMIV G'CCGG_C
Cuts at: 0 150 293 1742 2364
Size: 150 143 1449 622
Fragments arranged by size:
1449 622 150 143
NspI r_CATG'y
Cuts at: 0 412 641 733 2283 2364
Size: 412 229 92 1550 81
Fragments arranged by size:
1550 412 229 92 81
PciI A'CATG_T
Cuts at: 0 2279 2364
Size: 2279 85
PflMI CCAn_nnn'nTGG
Cuts at: 0 528 695 1547 1581 1689 2364
Size: 528 167 852 34 108 675
Fragments arranged by size:
852 675 528 167 108 34
PfoI T'CCnGG_A
Cuts at: 0 1595 2059 2364
Size: 1595 464 305
Fragments arranged by size:
1595 464 305
PpuMI rG'GwC_Cy
Cuts at: 0 330 1367 2052 2364
Size: 330 1037 685 312
Fragments arranged by size:
1037 685 330 312
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1309 1341 2364
Size: 1309 32 1023
Fragments arranged by size:
1309 1023 32
PstI C_TGCA'G
Cuts at: 0 292 1736 1824 2084 2105 2183 2364
Size: 292 1444 88 260 21 78 181
Fragments arranged by size:
1444 292 260 181 88 78 21
PvuII CAG'CTG
Cuts at: 0 536 1689 2364
Size: 536 1153 675
Fragments arranged by size:
1153 675 536
SalI G'TCGA_C
Cuts at: 0 6 2364
Size: 6 2358
SanDI GG'GwC_CC
Cuts at: 0 1367 2364
Size: 1367 997
SapI GCTCTTCn'nnn_
Cuts at: 0 463 820 1165 2364
Size: 463 357 345 1199
Fragments arranged by size:
1199 463 357 345
SexAI A'CCwGG_T
Cuts at: 0 1546 1580 1646 2364
Size: 1546 34 66 718
Fragments arranged by size:
1546 718 66 34
SfcI C'TryA_G
Cuts at: 0 288 1732 1820 2080 2101 2179 2364
Size: 288 1444 88 260 21 78 185
Fragments arranged by size:
1444 288 260 185 88 78 21
SfoI GGC'GCC
Cuts at: 0 1741 2364
Size: 1741 623
SgrAI Cr'CCGG_yG
Cuts at: 0 1742 2364
Size: 1742 622
SmaI CCC'GGG
Cuts at: 0 369 1228 2364
Size: 369 859 1136
Fragments arranged by size:
1136 859 369
SmlI C'TyrA_G
Cuts at: 0 1024 1483 1837 2364
Size: 1024 459 354 527
Fragments arranged by size:
1024 527 459 354
SphI G_CATG'C
Cuts at: 0 412 2364
Size: 412 1952
SrfI GCCC'GGGC
Cuts at: 0 1228 2364
Size: 1228 1136
StuI AGG'CCT
Cuts at: 0 1534 2364
Size: 1534 830
StyI C'CwwG_G
Cuts at: 0 694 935 1343 1496 1793 2364
Size: 694 241 408 153 297 571
Fragments arranged by size:
694 571 408 297 241 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 25 1894 2364
Size: 25 1869 470
Fragments arranged by size:
1869 470 25
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 906 2045 2364
Size: 906 1139 319
Fragments arranged by size:
1139 906 319
XmaI C'CCGG_G
Cuts at: 0 367 1226 2364
Size: 367 859 1138
Fragments arranged by size:
1138 859 367
ZraI GAC'GTC
Cuts at: 0 519 1182 2364
Size: 519 663 1182
Fragments arranged by size:
1182 663 519
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AleI AlfI AloI
AlwNI ApoI AvaI BamHI BanI BanII BbeI BbsI
BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BstEII BstXI BstYI BtgI BtsI DraIII DrdI EaeI
EarI EciI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HincII KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PpuMI PsrI
PstI PvuII SalI SanDI SapI SexAI SfcI SfoI
SgrAI SmaI SmlI SphI SrfI StuI StyI TaqII
XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI ApaI ApaLI
AscI AseI AsiSI AvrII BaeI BbvCI BcgI BciVI
BclI BfrBI BglI BlpI BmtI BsaI BsaAI BsiWI
BsmI BsmBI BsrGI BssHII BssSI BstAPI BstBI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI EagI EcoICRI EcoRI
EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI NdeI NheI NotI NruI NsiI PacI PasI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PvuI
RsrII SacI SacII SbfI ScaI SfiI SgfI SnaBI
SpeI SspI SwaI TatI TstI Tth111I XbaI XhoI
XmnI