Restriction map for
pFN21AF0175
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# NO CUT ENZYMES ( 60 enzymes) #
##################################
Acc65I AclI AflII AgeI Alw44I
ApaLI AscI AseI AsnI Asp718I
AvrII BcgI BciVI BclI BfrI
BlnI BsiWI BsmI Bsp1407I BsrGI
BssHII Bst1107I BstAPI BstBI BstZ17I
CpoI DraI EcoRI EcoRV EcoT22I
FbaI HpaI KpnI KspI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SapI SexAI SfiI SfuI
SgrAI SnaBI SpeI SspBI SwaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 2770 GACGTC GACGT^C
AccII 851 CGCG CG^CG
AflIII 3524 ACATGT A^C[GA][CT]GT
AhdI 4471 GACGGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1812 CCTTAGG CC^T[ACGT]AGG
Aor51HI 4035 AGCGCT AGC^GCT
AspEI 4471 GACGGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 5407 CACGTG CAC^GTG
BbvCI 2665 GCTGAGG CCTCAGC(-5/-2)
BsaAI 5407 CACGTG [CT]AC^GT[GA]
BsaI 1735 GAGACC GGTCTC(1/5)
BspHI 4524 TCATGA T^CATGA
BspLU11I 3524 ACATGT A^CATGT
BsrDI 5772 CATTGC GCAATG(2/0)
BstUI 851 CGCG CG^CG
Bsu36I 1812 CCTTAGG CC^T[ACGT]AGG
DraIII 4373 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4146 GACGGTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2816 CGGCCG C^GGCCG
Eam1105I 4471 GACGGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2816 CGGCCG C^GGCCG
Eco47III 4035 AGCGCT AGC^GCT
Eco52I 2816 CGGCCG C^GGCCG
Eco81I 1812 CCTTAGG CC^T[ACGT]AGG
FseI 539 GGCCGGCC GGCCGG^CC
HindIII 6157 AAGCTT A^AGCTT
MvnI 851 CGCG CG^CG
NcoI 509 CCATGG C^CATGG
PaeR7I 3295 CTCGAG C^TCGAG
PmaCI 5407 CACGTG CAC^GTG
PmlI 5407 CACGTG CAC^GTG
RcaI 4524 TCATGA T^CATGA
SbfI 357 CCTGCAGG CCTGCA^GG
ScaI 1826 AGTACT AGT^ACT
SphI 5909 GCATGC GCATG^C
Sse8387I 357 CCTGCAGG CCTGCA^GG
SspI 5297 AATATT AAT^ATT
StuI 4655 AGGCCT AGG^CCT
XbaI 721 TCTAGA T^CTAGA
XhoI 3295 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AviII 2282 TGCGCA TGC^GCA
3188 TGCGCA
BfaI 287 CTAG C^TAG
722 CTAG
BsaBI 1476 GATTCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3675 GATCGAGATC
BsiEI 2816 CGGCCG CG[GA][CT]^CG
5102 CGGTCG
BspDI 4270 ATCGAT AT^CGAT
4540 ATCGAT
BstEII 822 GGTTACC G^GT[ACGT]ACC
2887 GGTCACC
BstPI 822 GGTTACC G^GT[ACGT]ACC
2887 GGTCACC
ClaI 4270 ATCGAT AT^CGAT
4540 ATCGAT
EcoO65I 822 GGTTACC G^GT[ACGT]ACC
2887 GGTCACC
FspI 2282 TGCGCA TGC^GCA
3188 TGCGCA
MaeI 287 CTAG C^TAG
722 CTAG
MamI 1476 GATTCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3675 GATCGAGATC
MseI 3638 TTAA T^TAA
4751 TTAA
PshAI 2881 GACGCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5100 GACGGTCGTC
SalI 5107 GTCGAC G^TCGAC
5476 GTCGAC
Tru9I 3638 TTAA T^TAA
4751 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccI AccIII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApaI
ApoI Asp700I AspHI AspI AvaI
AvaII BalI BamHI BanI BanII
BbeI BbsI BbvI BcnI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspEI BspMI BsrBI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MroI MscI MslI MspA1I
MspI MvaI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmaCI
XmaI XmnI