(Circular) (Six-base) MAPSORT of: pFN21AF0175.seq Check: 2609 from: 1 to: 11062 pFN21AF0175 11062 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2356 2356 Size: 11062 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4779 8872 278 Size: 53 83 186 936 3243 4093 2468 Fragments arranged by size: 4093 3243 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2961 7113 7482 8405 1937 Size: 1024 4152 369 923 4594 Fragments arranged by size: 4594 4152 1024 923 369 Acc65I G'GTAC_C Cuts at: 8383 8383 Size: 11062 AclI AA'CG_TT Cuts at: 1563 9189 9562 1563 Size: 7626 373 3063 Fragments arranged by size: 7626 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3195 3269 4166 4352 6647 7370 8341 9125 Size: 1563 74 897 186 2295 723 971 784 Cuts at: 9125 10509 10777 1632 Size: 1384 268 1917 Fragments arranged by size: 2295 1917 1563 1384 971 897 784 723 268 186 74 AfeI AGC'GCT Cuts at: 6042 6042 Size: 11062 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10865 AflIII A'CryG_T Cuts at: 1203 5529 9961 10938 1203 Size: 4326 4432 977 1327 Fragments arranged by size: 4432 4326 1327 977 AgeI A'CCGG_T Cuts at: 9893 9893 Size: 11062 AhdI GACnn_n'nnGTC Cuts at: 6481 9791 6481 Size: 3310 7752 AleI CACnn'nnGTG Cuts at: 1166 4979 1166 Size: 3813 7249 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 11062 AlwNI CAG_nnn'CTG Cuts at: 3077 4228 4276 5711 7399 8805 10377 3077 Size: 1151 48 1435 1688 1406 1572 3762 Fragments arranged by size: 3762 1688 1572 1435 1406 1151 48 ApaI G_GGCC'C Cuts at: 3618 5404 7355 3618 Size: 1786 1951 7325 Fragments arranged by size: 7325 1951 1786 ApaLI G'TGCA_C Cuts at: 9119 10275 9119 Size: 1156 9906 ApoI r'AATT_y Cuts at: 1493 6560 6752 6771 8371 8597 8749 10858 1493 Size: 5067 192 19 1600 226 152 2109 1697 Fragments arranged by size: 5067 2109 1697 1600 226 192 152 19 AseI AT'TA_AT Cuts at: 160 1032 9616 160 Size: 872 8584 1606 Fragments arranged by size: 8584 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11062 AvaI C'yCGr_G Cuts at: 1870 1943 2638 2693 2993 5300 5541 8387 1870 Size: 73 695 55 300 2307 241 2846 4545 Fragments arranged by size: 4545 2846 2307 695 300 241 73 55 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 7250 7283 7250 Size: 33 11029 BamHI G'GATC_C Cuts at: 2859 5949 7332 8392 8759 2859 Size: 3090 1383 1060 367 5162 Fragments arranged by size: 5162 3090 1383 1060 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2787 3017 3990 4483 4517 Size: 325 217 627 1000 230 973 493 34 Cuts at: 4517 4585 4592 5340 7258 8178 8383 9838 618 Size: 68 7 748 1918 920 205 1455 1842 Fragments arranged by size: 1918 1842 1455 1000 973 920 748 627 493 325 230 217 205 68 34 7 BanII G_rGCy'C Cuts at: 1391 2195 2430 2647 2943 3036 3238 3618 3684 Size: 804 235 217 296 93 202 380 66 Cuts at: 3684 4206 4353 4416 4638 5404 5886 6246 6453 Size: 522 147 63 222 766 482 360 207 Cuts at: 6453 6996 7150 7355 7892 8148 8381 1391 Size: 543 154 205 537 256 233 4072 Fragments arranged by size: 4072 804 766 543 537 522 482 380 360 296 256 235 233 222 217 207 205 202 154 147 93 66 63 BbeI G_GCGC'C Cuts at: 2791 4596 5344 8182 2791 Size: 1805 748 2838 5671 Fragments arranged by size: 5671 2838 1805 748 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2580 3183 3247 3413 4871 5372 5624 Size: 971 681 603 64 166 1458 501 252 Cuts at: 5624 6426 6795 928 Size: 802 369 5195 Fragments arranged by size: 5195 1458 971 802 681 603 501 369 252 166 64 BbvCI CC'TCA_GC Cuts at: 4671 4671 Size: 11062 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 9253 9287 1903 Size: 34 50 34 7232 34 3678 Fragments arranged by size: 7232 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 8954 10164 8954 Size: 1210 9852 BclI T'GATC_A Cuts at: 1548 1548 Size: 11062 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4148 5028 6291 Size: 107 122 71 631 605 2476 880 1263 Cuts at: 6291 9673 136 Size: 3382 1525 Fragments arranged by size: 3382 2476 1525 1263 880 631 605 122 107 71 BglII A'GATC_T Cuts at: 3312 3396 5685 6933 7848 11057 3312 Size: 84 2289 1248 915 3209 3317 Fragments arranged by size: 3317 3209 2289 1248 915 84 BlpI GC'TnA_GC Cuts at: 4188 5712 7761 8479 4188 Size: 1524 2049 718 6771 Fragments arranged by size: 6771 2049 1524 718 Bme1580I G_kGCm'C Cuts at: 1790 2400 3020 3618 4402 4488 4745 5024 5404 Size: 610 620 598 784 86 257 279 380 Cuts at: 5404 7355 9123 10279 1790 Size: 1951 1768 1156 2573 Fragments arranged by size: 2573 1951 1768 1156 784 620 610 598 380 279 257 86 BmgBI CAC'GTC Cuts at: 1318 2675 3013 4171 6424 7687 1318 Size: 1357 338 1158 2253 1263 4693 Fragments arranged by size: 4693 2253 1357 1263 1158 338 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2037 3505 4060 Size: 416 53 83 399 24 620 1468 555 Cuts at: 4060 4747 5036 5146 7349 8793 9751 442 Size: 687 289 110 2203 1444 958 1753 Fragments arranged by size: 2203 1753 1468 1444 958 687 620 555 416 399 289 110 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 11062 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2297 4267 5290 2297 Size: 1970 1023 8069 Fragments arranged by size: 8069 1970 1023 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2409 2444 2619 3176 3585 3758 4145 4385 Size: 1170 35 175 557 409 173 387 240 Cuts at: 4385 4860 5381 6029 6353 6626 6668 7010 7058 Size: 475 521 648 324 273 42 342 48 Cuts at: 7058 7139 7747 7819 9722 1239 Size: 81 608 72 1903 2579 Fragments arranged by size: 2579 1903 1170 648 608 557 521 475 409 387 342 324 273 240 175 173 81 72 48 42 35 Bpu10I CC'TnA_GC Cuts at: 4301 4671 4898 7604 8160 4301 Size: 370 227 2706 556 7203 Fragments arranged by size: 7203 2706 556 370 227 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2153 5414 5476 8186 9187 10052 10350 2153 Size: 3261 62 2710 1001 865 298 2865 Fragments arranged by size: 3261 2865 2710 1001 865 298 62 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3734 9725 882 Size: 617 2235 5991 2219 Fragments arranged by size: 5991 2235 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 7414 8853 493 Size: 711 6210 1439 2702 Fragments arranged by size: 6210 2702 1439 711 BsaBI GATnn'nnATC Cuts at: 3485 5684 11056 3485 Size: 2199 5372 3491 Fragments arranged by size: 5372 3491 2199 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2788 3885 4593 4776 Size: 53 83 186 936 1255 1097 708 183 Cuts at: 4776 4887 5341 8179 8869 9251 9886 275 Size: 111 454 2838 690 382 635 1451 Fragments arranged by size: 2838 1451 1255 1097 936 708 690 635 454 382 186 183 111 83 53 BsaWI w'CCGG_w Cuts at: 3003 4858 6180 7269 8250 9495 9893 10167 10314 Size: 1855 1322 1089 981 1245 398 274 147 Cuts at: 10314 10725 3003 Size: 411 3340 Fragments arranged by size: 3340 1855 1322 1245 1089 981 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 6101 6131 6496 6526 7064 Size: 30 165 30 4698 30 365 30 538 Cuts at: 7064 7094 7853 7883 1178 Size: 30 759 30 4357 Fragments arranged by size: 4698 4357 759 538 365 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2409 2580 2981 3246 3582 4424 6106 6254 Size: 1223 171 401 265 336 842 1682 148 Cuts at: 6254 6504 6737 7067 7214 7376 8129 1186 Size: 250 233 330 147 162 753 4119 Fragments arranged by size: 4119 1682 1223 842 753 401 336 330 265 250 233 171 162 148 147 BseYI C'CCAG_C Cuts at: 3092 3130 5091 5181 5262 5723 6122 6793 7218 Size: 38 1961 90 81 461 399 671 425 Cuts at: 7218 7828 10265 10761 3092 Size: 610 2437 496 3393 Fragments arranged by size: 3393 2437 1961 671 610 496 461 425 399 90 81 38 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3409 3996 4628 5588 5784 5954 6176 Size: 573 1673 587 632 960 196 170 222 Cuts at: 6176 6190 6967 7009 1163 Size: 14 777 42 5216 Fragments arranged by size: 5216 1673 960 777 632 587 573 222 196 170 42 14 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4824 7110 8512 9273 10301 1540 Size: 275 186 2823 2286 1402 761 1028 2301 Fragments arranged by size: 2823 2301 2286 1402 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3684 3759 4160 4206 4353 5886 6246 6429 7413 Size: 75 401 46 147 1533 360 183 984 Cuts at: 7413 7949 9123 9208 10279 3684 Size: 536 1174 85 1071 4467 Fragments arranged by size: 4467 1533 1174 1071 984 536 401 360 183 147 85 75 46 BsmI GAATG_Cn' Cuts at: 8573 8666 8573 Size: 93 10969 BsmBI CGTCTCn'nnnn_ Cuts at: 2219 5452 5599 7097 2219 Size: 3233 147 1498 6184 Fragments arranged by size: 6184 3233 1498 147 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2195 2400 2430 2647 2943 3020 3036 Size: 399 405 205 30 217 296 77 16 Cuts at: 3036 3238 3618 3684 3759 4160 4206 4353 4402 Size: 202 380 66 75 401 46 147 49 Cuts at: 4402 4416 4488 4638 4745 5024 5404 5886 6246 Size: 14 72 150 107 279 380 482 360 Cuts at: 6246 6429 6453 6996 7150 7355 7413 7892 7949 Size: 183 24 543 154 205 58 479 57 Cuts at: 7949 8148 8381 9123 9208 10279 1391 Size: 199 233 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 543 482 479 405 401 399 380 380 360 296 279 233 217 205 205 202 199 183 154 150 147 107 85 77 75 72 66 58 57 49 46 30 24 16 14 BspEI T'CCGG_A Cuts at: 3003 4858 6180 10725 3003 Size: 1855 1322 4545 3340 Fragments arranged by size: 4545 3340 1855 1322 BspHI T'CATG_A Cuts at: 6529 8951 10681 6529 Size: 2422 1730 6910 Fragments arranged by size: 6910 2422 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2356 3210 3947 4120 5985 7031 7761 8417 Size: 1512 854 737 173 1865 1046 730 656 Cuts at: 8417 844 Size: 3489 Fragments arranged by size: 3489 1865 1512 1046 854 737 730 656 173 BsrBI CCG'CTC Cuts at: 1394 1823 5122 6327 8119 8949 1394 Size: 429 3299 1205 1792 830 3507 Fragments arranged by size: 3507 3299 1792 1205 830 429 BsrDI GCAATG_nn' Cuts at: 65 1248 7776 9557 9731 65 Size: 1183 6528 1781 174 1396 Fragments arranged by size: 6528 1781 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2058 2545 3720 4107 4823 4889 5698 Size: 110 248 487 1175 387 716 66 809 Cuts at: 5698 5902 6297 9706 9893 1700 Size: 204 395 3409 187 2869 Fragments arranged by size: 3409 2869 1175 809 716 487 395 387 248 204 187 110 66 BsrGI T'GTAC_A Cuts at: 96 96 Size: 11062 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 11062 BssSI C'ACGA_G Cuts at: 2521 6128 7833 9122 10134 2521 Size: 3607 1705 1289 1012 3449 Fragments arranged by size: 3607 3449 1705 1289 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 11062 BstBI TT'CG_AA Cuts at: 9866 9866 Size: 11062 BstEII G'GTnAC_C Cuts at: 1184 2827 4892 1184 Size: 1643 2065 7354 Fragments arranged by size: 7354 2065 1643 BstXI CCAn_nnnn'nTGG Cuts at: 3751 4094 4264 5031 5731 5921 6130 8344 3751 Size: 343 170 767 700 190 209 2214 6469 Fragments arranged by size: 6469 2214 767 700 343 209 190 170 BstYI r'GATC_y Cuts at: 1972 2859 3312 3396 4953 5203 5685 5949 6733 Size: 887 453 84 1557 250 482 264 784 Cuts at: 6733 6933 7332 7848 8392 8759 9144 9161 10613 Size: 200 399 516 544 367 385 17 1452 Cuts at: 10613 10699 10711 11057 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1557 1452 887 784 544 516 482 453 399 385 367 346 264 250 200 86 84 17 12 Bsu36I CC'TnA_GG Cuts at: 3818 3818 Size: 11062 BtgI C'CryG_G Cuts at: 513 1065 2514 2553 4161 5816 7535 513 Size: 552 1449 39 1608 1655 1719 4040 Fragments arranged by size: 4040 1719 1655 1608 1449 552 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4192 5570 487 Size: 683 56 2966 1378 5979 Fragments arranged by size: 5979 2966 1378 683 56 BtsI GCAGTG_nn' Cuts at: 3440 4160 8578 9372 9392 3440 Size: 720 4418 794 20 5110 Fragments arranged by size: 5110 4418 794 720 20 ClaI AT'CG_AT Cuts at: 1554 6276 6546 1554 Size: 4722 270 6070 Fragments arranged by size: 6070 4722 270 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4510 4522 4545 4557 529 Size: 35 3946 12 23 12 7034 Fragments arranged by size: 7034 3946 35 23 12 12 DraI TTT'AAA Cuts at: 8366 8366 Size: 11062 DraIII CAC_nnn'GTG Cuts at: 6383 6383 Size: 11062 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6157 10069 809 Size: 507 4841 3912 1802 Fragments arranged by size: 4841 3912 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2543 3411 3718 Size: 54 1425 50 275 18 713 868 307 Cuts at: 3718 4339 4821 5700 5904 6105 6292 6831 8128 Size: 621 482 879 204 201 187 539 1297 Cuts at: 8128 8301 8509 9398 11027 8 Size: 173 208 889 1629 43 Fragments arranged by size: 1629 1425 1297 889 879 868 713 621 539 482 307 275 208 204 201 187 173 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4821 8509 1537 Size: 275 3009 3688 4090 Fragments arranged by size: 4090 3688 3009 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2099 2888 4508 4905 6086 6218 6531 6770 Size: 746 789 1620 397 1181 132 313 239 Cuts at: 6770 7238 7308 8037 8273 8285 8992 1353 Size: 468 70 729 236 12 707 3423 Fragments arranged by size: 3423 1620 1181 789 746 729 707 468 397 313 239 236 132 70 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2290 2624 2670 4388 4802 5258 Size: 1064 138 701 334 46 1718 414 456 Cuts at: 5258 5264 5315 6377 7199 9649 10023 10169 387 Size: 6 51 1062 822 2450 374 146 1280 Fragments arranged by size: 2450 1718 1280 1064 1062 822 701 456 414 374 334 146 138 51 46 6 EcoICRI GAG'CTC Cuts at: 3682 4204 4351 5884 6244 3682 Size: 522 147 1533 360 8500 Fragments arranged by size: 8500 1533 522 360 147 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2409 2444 2619 3176 3195 3269 3585 Size: 393 777 35 175 557 19 74 316 Cuts at: 3585 3758 4145 4166 4352 4385 4860 5381 6029 Size: 173 387 21 186 33 475 521 648 Cuts at: 6029 6353 6626 6647 6668 7010 7058 7139 7370 Size: 324 273 21 21 342 48 81 231 Cuts at: 7370 7747 7819 8341 9125 9722 10509 10777 1239 Size: 377 72 522 784 597 787 268 1524 Fragments arranged by size: 1524 787 784 777 648 597 557 522 521 475 393 387 377 342 324 316 273 268 231 186 175 173 81 74 72 48 35 33 21 21 21 19 EcoNI CCTnn'n_nnAGG Cuts at: 2186 3049 4127 4406 6608 7082 7798 2186 Size: 863 1078 279 2202 474 716 5450 Fragments arranged by size: 5450 2202 1078 863 716 474 279 EcoO109I rG'GnC_Cy Cuts at: 2164 2263 2278 2349 3134 3615 3778 5140 5372 Size: 99 15 71 785 481 163 1362 232 Cuts at: 5372 5400 5401 5545 6076 6670 6760 6842 6901 Size: 28 1 144 531 594 90 82 59 Cuts at: 6901 7045 7396 7498 8170 2164 Size: 144 351 102 672 5056 Fragments arranged by size: 5056 1362 785 672 594 531 481 351 232 163 144 144 102 99 90 82 71 59 28 15 1 EcoRI G'AATT_C Cuts at: 8371 8749 8371 Size: 378 10684 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2103 3358 4825 6781 7810 7888 7966 2103 Size: 1255 1467 1956 1029 78 78 5199 Fragments arranged by size: 5199 1956 1467 1255 1029 78 78 FseI GG_CCGG'CC Cuts at: 2549 2549 Size: 11062 FspI TGC'GCA Cuts at: 4289 5195 9568 4289 Size: 906 4373 5783 Fragments arranged by size: 5783 4373 906 HaeII r_GCGC'y Cuts at: 1678 2791 3152 4596 5182 5344 6044 8182 10209 Size: 1113 361 1444 586 162 700 2138 2027 Cuts at: 10209 1678 Size: 2531 Fragments arranged by size: 2531 2138 2027 1444 1113 700 586 361 162 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2177 2209 2477 2509 2971 Size: 32 135 32 605 32 268 32 462 Cuts at: 2971 3003 3071 3103 4160 4192 5039 5071 5426 Size: 32 68 32 1057 32 847 32 355 Cuts at: 5426 5458 5552 5584 6341 6356 6373 6388 6467 Size: 32 94 32 757 15 17 15 79 Cuts at: 6467 6499 6659 6691 6718 6750 7012 7044 7064 Size: 32 160 32 27 32 262 32 20 Cuts at: 7064 7096 7575 7607 7853 7885 9703 9735 9777 Size: 32 479 32 246 32 1818 32 42 Cuts at: 9777 9809 1373 Size: 32 2626 Fragments arranged by size: 2626 1818 1057 847 757 605 479 462 355 268 262 246 160 135 94 79 68 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 27 20 17 15 15 HincII GTy'rAC Cuts at: 669 1657 1938 5795 7114 7483 8406 8652 669 Size: 988 281 3857 1319 369 923 246 3079 Fragments arranged by size: 3857 3079 1319 988 923 369 281 246 HindIII A'AGCT_T Cuts at: 748 8162 748 Size: 7414 3648 HpaI GTT'AAC Cuts at: 8652 8652 Size: 11062 KasI G'GCGC_C Cuts at: 2787 4592 5340 8178 2787 Size: 1805 748 2838 5671 Fragments arranged by size: 5671 2838 1805 748 KpnI G_GTAC'C Cuts at: 8387 8387 Size: 11062 MfeI C'AATT_G Cuts at: 8661 8661 Size: 11062 MluI A'CGCG_T Cuts at: 10938 10938 Size: 11062 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7628 10176 10360 1305 Size: 6323 2548 184 2007 Fragments arranged by size: 6323 2548 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 6107 8130 8303 10 Size: 54 1425 343 4275 2023 173 2769 Fragments arranged by size: 4275 2769 2023 1425 343 173 54 MslI CAynn'nnrTG Cuts at: 518 1166 4445 4979 5732 6014 6221 6803 7637 Size: 648 3279 534 753 282 207 582 834 Cuts at: 7637 9020 9379 9538 518 Size: 1383 359 159 2042 Fragments arranged by size: 3279 2042 1383 834 753 648 582 534 359 282 207 159 MspA1I CmG'CkG Cuts at: 1606 2383 2794 2834 3949 4090 4192 4285 4480 Size: 777 411 40 1115 141 102 93 195 Cuts at: 4480 5337 5381 6103 6646 6829 7760 7960 8478 Size: 857 44 722 543 183 931 200 518 Cuts at: 8478 9155 10303 10548 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 1115 931 857 777 722 677 543 518 411 245 200 195 183 141 102 93 44 40 NaeI GCC'GGC Cuts at: 2547 3722 4825 5700 5904 6299 2547 Size: 1175 1103 875 204 395 7310 Fragments arranged by size: 7310 1175 1103 875 395 204 NarI GG'CG_CC Cuts at: 2788 4593 5341 8179 2788 Size: 1805 748 2838 5671 Fragments arranged by size: 5671 2838 1805 748 NcoI C'CATG_G Cuts at: 513 1065 2514 513 Size: 552 1449 9061 Fragments arranged by size: 9061 1449 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 11062 NgoMIV G'CCGG_C Cuts at: 2545 3720 4823 5698 5902 6297 2545 Size: 1175 1103 875 204 395 7310 Fragments arranged by size: 7310 1175 1103 875 395 204 NheI G'CTAG_C Cuts at: 1052 1052 Size: 11062 NotI GC'GGCC_GC Cuts at: 8509 8509 Size: 11062 NspI r_CATG'y Cuts at: 1132 1229 5533 7382 7918 8420 9965 1132 Size: 97 4304 1849 536 502 1545 2229 Fragments arranged by size: 4304 2229 1849 1545 536 502 97 PasI CC'CwG_GG Cuts at: 1348 4011 5404 6234 6838 7501 7702 1348 Size: 2663 1393 830 604 663 201 4708 Fragments arranged by size: 4708 2663 1393 830 663 604 201 PciI A'CATG_T Cuts at: 5529 9961 5529 Size: 4432 6630 PflMI CCAn_nnn'nTGG Cuts at: 1108 4228 4882 6383 6829 1108 Size: 3120 654 1501 446 5341 Fragments arranged by size: 5341 3120 1501 654 446 PfoI T'CCnGG_A Cuts at: 2619 4939 5952 7404 7593 2619 Size: 2320 1013 1452 189 6088 Fragments arranged by size: 6088 2320 1452 1013 189 PmeI GTTT'AAAC Cuts at: 8366 8366 Size: 11062 PmlI CAC'GTG Cuts at: 7414 7414 Size: 11062 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4640 4672 6524 6556 9131 9163 10644 10676 4640 Size: 32 1852 32 2575 32 1481 32 5026 Fragments arranged by size: 5026 2575 1852 1481 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2164 2263 2278 3778 5140 5545 6842 6901 7045 Size: 99 15 1500 1362 405 1297 59 144 Cuts at: 7045 8170 2164 Size: 1125 5056 Fragments arranged by size: 5056 1500 1362 1297 1125 405 144 99 59 15 PshAI GACnn'nnGTC Cuts at: 1613 4890 7109 1613 Size: 3277 2219 5566 Fragments arranged by size: 5566 3277 2219 PsiI TTA'TAA Cuts at: 8632 8632 Size: 11062 PspOMI G'GGCC_C Cuts at: 3614 5400 7351 3614 Size: 1786 1951 7325 Fragments arranged by size: 7325 1951 1786 PspXI vC'TCGA_Gb Cuts at: 5300 5300 Size: 11062 PstI C_TGCA'G Cuts at: 830 2367 2839 3873 4220 4360 4455 5380 6894 Size: 1537 472 1034 347 140 95 925 1514 Cuts at: 6894 7446 7473 7899 8414 830 Size: 552 27 426 515 3478 Fragments arranged by size: 3478 1537 1514 1034 925 552 515 472 426 347 140 95 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11062 PvuII CAG'CTG Cuts at: 2794 4090 4192 4285 5381 6103 6646 6829 7760 Size: 1296 102 93 1096 722 543 183 931 Cuts at: 7760 7960 2794 Size: 200 5896 Fragments arranged by size: 5896 1296 1096 931 722 543 200 183 102 93 SacI G_AGCT'C Cuts at: 3684 4206 4353 5886 6246 3684 Size: 522 147 1533 360 8500 Fragments arranged by size: 8500 1533 522 360 147 SalI G'TCGA_C Cuts at: 1936 7112 7481 8404 1936 Size: 5176 369 923 4594 Fragments arranged by size: 5176 4594 923 369 SanDI GG'GwC_CC Cuts at: 5140 6842 5140 Size: 1702 9360 SbfI CC_TGCA'GG Cuts at: 2367 8414 2367 Size: 6047 5015 ScaI AGT'ACT Cuts at: 3833 9310 11022 3833 Size: 5477 1712 3873 Fragments arranged by size: 5477 3873 1712 SfcI C'TryA_G Cuts at: 826 1045 2363 2835 3869 4216 4356 4451 4550 Size: 219 1318 472 1034 347 140 95 99 Cuts at: 4550 5376 6890 7432 7442 7469 7895 8410 9545 Size: 826 1514 542 10 27 426 515 1135 Cuts at: 9545 10226 10417 826 Size: 681 191 1471 Fragments arranged by size: 1514 1471 1318 1135 1034 826 681 542 515 472 426 347 219 191 140 99 95 27 10 SfoI GGC'GCC Cuts at: 2789 4594 5342 8180 2789 Size: 1805 748 2838 5671 Fragments arranged by size: 5671 2838 1805 748 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11062 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 11062 SmaI CCC'GGG Cuts at: 1872 2640 2695 5543 8389 1872 Size: 768 55 2848 2846 4545 Fragments arranged by size: 4545 2848 2846 768 55 SmlI C'TyrA_G Cuts at: 820 1017 1943 2168 5300 5393 5455 8165 9166 Size: 197 926 225 3132 93 62 2710 1001 Cuts at: 9166 10067 10329 820 Size: 901 262 1553 Fragments arranged by size: 3132 2710 1553 1001 926 901 262 225 197 93 62 SnaBI TAC'GTA Cuts at: 493 8853 493 Size: 8360 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 11062 SphI G_CATG'C Cuts at: 1132 7918 8420 1132 Size: 6786 502 3774 Fragments arranged by size: 6786 3774 502 SspI AAT'ATT Cuts at: 5 52 7304 8986 5 Size: 47 7252 1682 2081 Fragments arranged by size: 7252 2081 1682 47 StuI AGG'CCT Cuts at: 1517 6662 1517 Size: 5145 5917 StyI C'CwwG_G Cuts at: 513 1065 2259 2514 2657 4800 5004 6897 7083 Size: 552 1194 255 143 2143 204 1893 186 Cuts at: 7083 7950 8085 8174 8501 513 Size: 867 135 89 327 3074 Fragments arranged by size: 3074 2143 1893 1194 867 552 327 255 204 186 143 135 89 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4220 4274 4307 5173 7201 9089 9106 Size: 1255 2640 54 33 866 2028 1888 17 Cuts at: 9106 9259 9444 9889 325 Size: 153 185 445 1498 Fragments arranged by size: 2640 2028 1888 1498 1255 866 445 185 153 54 33 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3831 3966 5157 Size: 274 80 33 51 1443 1854 135 1191 Cuts at: 5157 9308 11020 96 Size: 4151 1712 138 Fragments arranged by size: 4151 1854 1712 1443 1191 274 138 135 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6496 6528 7264 7296 11022 11054 6496 Size: 32 736 32 3726 32 6504 Fragments arranged by size: 6504 3726 736 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2072 2491 4436 1316 Size: 756 419 1945 7942 Fragments arranged by size: 7942 1945 756 419 XbaI T'CTAG_A Cuts at: 2726 8398 2726 Size: 5672 5390 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2594 4168 5731 5756 5823 5918 6013 6064 6328 Size: 1574 1563 25 67 95 95 51 264 Cuts at: 6328 2594 Size: 7328 Fragments arranged by size: 7328 1574 1563 264 95 95 67 51 25 XhoI C'TCGA_G Cuts at: 1943 5300 1943 Size: 3357 7705 XmaI C'CCGG_G Cuts at: 1870 2638 2693 5541 8387 1870 Size: 768 55 2848 2846 4545 Fragments arranged by size: 4545 2848 2846 768 55 XmnI GAAnn'nnTTC Cuts at: 2243 3254 3926 4655 8354 9191 2243 Size: 1011 672 729 3699 837 4114 Fragments arranged by size: 4114 3699 1011 837 729 672 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4777 8870 276 Size: 53 83 186 936 3243 4093 2468 Fragments arranged by size: 4093 3243 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BfrBI BsiWI BstZ17I EcoRV FspAI NruI NsiI PacI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI