Restriction map for
pFN21AF0175
(Linear) (Six-base) MAPSORT of: FLJ00175.seq Check: 6858 from: 1 to: 6357
FLJ00175.orf
With 182 enzymes: SgfI *
April 24, 2009 13:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 351 6357
Size: 351 6006
AatII G_ACGT'C
Cuts at: 0 2774 6357
Size: 2774 3583
AccI GT'mk_AC
Cuts at: 0 956 5108 5477 6357
Size: 956 4152 369 880
Fragments arranged by size:
4152 956 880 369
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1190 1264 2161 2347 4642 5365 6336 6357
Size: 1190 74 897 186 2295 723 971 21
Fragments arranged by size:
2295 1190 971 897 723 186 74 21
AfeI AGC'GCT
Cuts at: 0 4037 6357
Size: 4037 2320
AflIII A'CryG_T
Cuts at: 0 3524 6357
Size: 3524 2833
AhdI GACnn_n'nnGTC
Cuts at: 0 4476 6357
Size: 4476 1881
AleI CACnn'nnGTG
Cuts at: 0 2974 6357
Size: 2974 3383
AlwNI CAG_nnn'CTG
Cuts at: 0 1072 2223 2271 3706 5394 6357
Size: 1072 1151 48 1435 1688 963
Fragments arranged by size:
1688 1435 1151 1072 963 48
ApaI G_GGCC'C
Cuts at: 0 1613 3399 5350 6357
Size: 1613 1786 1951 1007
Fragments arranged by size:
1951 1786 1613 1007
ApoI r'AATT_y
Cuts at: 0 4555 4747 4766 6357
Size: 4555 192 19 1591
Fragments arranged by size:
4555 1591 192 19
AvaI C'yCGr_G
Cuts at: 0 633 688 988 3295 3536 6357
Size: 633 55 300 2307 241 2821
Fragments arranged by size:
2821 2307 633 300 241 55
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 5245 5278 6357
Size: 5245 33 1079
Fragments arranged by size:
5245 1079 33
BamHI G'GATC_C
Cuts at: 0 854 3944 5327 6357
Size: 854 3090 1383 1030
Fragments arranged by size:
3090 1383 1030 854
BanI G'GyrC_C
Cuts at: 0 782 1012 1985 2478 2512 2580 2587 3335
Size: 782 230 973 493 34 68 7 748
Cuts at: 3335 5253 6173 6357
Size: 1918 920 184
Fragments arranged by size:
1918 973 920 782 748 493 230 184
68 34 7
BanII G_rGCy'C
Cuts at: 0 190 425 642 938 1031 1233 1613 1679
Size: 190 235 217 296 93 202 380 66
Cuts at: 1679 2201 2348 2411 2633 3399 3881 4241 4448
Size: 522 147 63 222 766 482 360 207
Cuts at: 4448 4991 5145 5350 5887 6143 6357
Size: 543 154 205 537 256 214
Fragments arranged by size:
766 543 537 522 482 380 360 296
256 235 222 217 214 207 205 202
190 154 147 93 66 63
BbeI G_GCGC'C
Cuts at: 0 786 2591 3339 6177 6357
Size: 786 1805 748 2838 180
Fragments arranged by size:
2838 1805 786 748 180
BbsI GAAGACnn'nnnn_
Cuts at: 0 575 1178 1242 1408 2866 3367 3619 4421
Size: 575 603 64 166 1458 501 252 802
Cuts at: 4421 4790 6357
Size: 369 1567
Fragments arranged by size:
1567 1458 802 603 575 501 369 252
166 64
BbvCI CC'TCA_GC
Cuts at: 0 2666 6357
Size: 2666 3691
BglI GCCn_nnn'nGGC
Cuts at: 0 2143 3023 4286 6357
Size: 2143 880 1263 2071
Fragments arranged by size:
2143 2071 1263 880
BglII A'GATC_T
Cuts at: 0 1307 1391 3680 4928 5843 6357
Size: 1307 84 2289 1248 915 514
Fragments arranged by size:
2289 1307 1248 915 514 84
BlpI GC'TnA_GC
Cuts at: 0 2183 3707 5756 6357
Size: 2183 1524 2049 601
Fragments arranged by size:
2183 2049 1524 601
Bme1580I G_kGCm'C
Cuts at: 0 395 1015 1613 2397 2483 2740 3019 3399
Size: 395 620 598 784 86 257 279 380
Cuts at: 3399 5350 6357
Size: 1951 1007
Fragments arranged by size:
1951 1007 784 620 598 395 380 279
257 86
BmgBI CAC'GTC
Cuts at: 0 670 1008 2166 4419 5682 6357
Size: 670 338 1158 2253 1263 675
Fragments arranged by size:
2253 1263 1158 675 670 338
BmrI ACTGGGnnnn_n'
Cuts at: 0 32 1500 2055 2742 3031 3141 5344 6357
Size: 32 1468 555 687 289 110 2203 1013
Fragments arranged by size:
2203 1468 1013 687 555 289 110 32
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 292 2262 3285 6357
Size: 292 1970 1023 3072
Fragments arranged by size:
3072 1970 1023 292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 439 614 1171 1580 1753 2140 2380
Size: 404 35 175 557 409 173 387 240
Cuts at: 2380 2855 3376 4024 4348 4621 4663 5005 5053
Size: 475 521 648 324 273 42 342 48
Cuts at: 5053 5134 5742 5814 6357
Size: 81 608 72 543
Fragments arranged by size:
648 608 557 543 521 475 409 404
387 342 324 273 240 175 173 81
72 48 42 35
Bpu10I CC'TnA_GC
Cuts at: 0 2296 2666 2893 5599 6155 6357
Size: 2296 370 227 2706 556 202
Fragments arranged by size:
2706 2296 556 370 227 202
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 3409 3471 6181 6357
Size: 148 3261 62 2710 176
Fragments arranged by size:
3261 2710 176 148 62
BsaI GGTCTCn'nnnn_
Cuts at: 0 1729 6357
Size: 1729 4628
BsaAI yAC'GTr
Cuts at: 0 5409 6357
Size: 5409 948
BsaBI GATnn'nnATC
Cuts at: 0 1480 3679 6357
Size: 1480 2199 2678
Fragments arranged by size:
2678 2199 1480
BsaHI Gr'CG_yC
Cuts at: 0 783 1880 2588 2771 2882 3336 6174 6357
Size: 783 1097 708 183 111 454 2838 183
Fragments arranged by size:
2838 1097 783 708 454 183 183 111
BsaWI w'CCGG_w
Cuts at: 0 998 2853 4175 5264 6245 6357
Size: 998 1855 1322 1089 981 112
Fragments arranged by size:
1855 1322 1089 998 981 112
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4096 4126 4491 4521 5059 5089 5848 5878
Size: 4096 30 365 30 538 30 759 30
Cuts at: 5878 6357
Size: 479
Fragments arranged by size:
4096 759 538 479 365 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 404 575 976 1241 1577 2419 4101 4249
Size: 404 171 401 265 336 842 1682 148
Cuts at: 4249 4499 4732 5062 5209 5371 6124 6357
Size: 250 233 330 147 162 753 233
Fragments arranged by size:
1682 842 753 404 401 336 330 265
250 233 233 171 162 148 147
BseYI C'CCAG_C
Cuts at: 0 1087 1125 3086 3176 3257 3718 4117 4788
Size: 1087 38 1961 90 81 461 399 671
Cuts at: 4788 5213 5823 6357
Size: 425 610 534
Fragments arranged by size:
1961 1087 671 610 534 461 425 399
90 81 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1404 1991 2623 3583 3779 3949 4171 4185
Size: 1404 587 632 960 196 170 222 14
Cuts at: 4185 4962 5004 6357
Size: 777 42 1353
Fragments arranged by size:
1404 1353 960 777 632 587 222 196
170 42 14
BsiEI CG_ry'CG
Cuts at: 0 2819 5105 6357
Size: 2819 2286 1252
Fragments arranged by size:
2819 2286 1252
BsiHKAI G_wGCw'C
Cuts at: 0 1679 1754 2155 2201 2348 3881 4241 4424
Size: 1679 75 401 46 147 1533 360 183
Cuts at: 4424 5408 5944 6357
Size: 984 536 413
Fragments arranged by size:
1679 1533 984 536 413 401 360 183
147 75 46
BsmBI CGTCTCn'nnnn_
Cuts at: 0 214 3447 3594 5092 6357
Size: 214 3233 147 1498 1265
Fragments arranged by size:
3233 1498 1265 214 147
Bsp1286I G_dGCh'C
Cuts at: 0 190 395 425 642 938 1015 1031 1233
Size: 190 205 30 217 296 77 16 202
Cuts at: 1233 1613 1679 1754 2155 2201 2348 2397 2411
Size: 380 66 75 401 46 147 49 14
Cuts at: 2411 2483 2633 2740 3019 3399 3881 4241 4424
Size: 72 150 107 279 380 482 360 183
Cuts at: 4424 4448 4991 5145 5350 5408 5887 5944 6143
Size: 24 543 154 205 58 479 57 199
Cuts at: 6143 6357
Size: 214
Fragments arranged by size:
543 482 479 401 380 380 360 296
279 217 214 205 205 202 199 190
183 154 150 147 107 77 75 72
66 58 57 49 46 30 24 16
14
BspEI T'CCGG_A
Cuts at: 0 998 2853 4175 6357
Size: 998 1855 1322 2182
Fragments arranged by size:
2182 1855 1322 998
BspHI T'CATG_A
Cuts at: 0 4524 6357
Size: 4524 1833
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 351 1205 1942 2115 3980 5026 5756 6357
Size: 351 854 737 173 1865 1046 730 601
Fragments arranged by size:
1865 1046 854 737 730 601 351 173
BsrBI CCG'CTC
Cuts at: 0 3117 4322 6114 6357
Size: 3117 1205 1792 243
Fragments arranged by size:
3117 1792 1205 243
BsrDI GCAATG_nn'
Cuts at: 0 5771 6357
Size: 5771 586
BsrFI r'CCGG_y
Cuts at: 0 53 540 1715 2102 2818 2884 3693 3897
Size: 53 487 1175 387 716 66 809 204
Cuts at: 3897 4292 6357
Size: 395 2065
Fragments arranged by size:
2065 1175 809 716 487 395 387 204
66 53
BssSI C'ACGA_G
Cuts at: 0 516 4123 5828 6357
Size: 516 3607 1705 529
Fragments arranged by size:
3607 1705 529 516
BstEII G'GTnAC_C
Cuts at: 0 822 2887 6357
Size: 822 2065 3470
Fragments arranged by size:
3470 2065 822
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1746 2089 2259 3026 3726 3916 4125 6339
Size: 1746 343 170 767 700 190 209 2214
Cuts at: 6339 6357
Size: 18
Fragments arranged by size:
2214 1746 767 700 343 209 190 170
18
BstYI r'GATC_y
Cuts at: 0 854 1307 1391 2948 3198 3680 3944 4728
Size: 854 453 84 1557 250 482 264 784
Cuts at: 4728 4928 5327 5843 6357
Size: 200 399 516 514
Fragments arranged by size:
1557 854 784 516 514 482 453 399
264 250 200 84
Bsu36I CC'TnA_GG
Cuts at: 0 1813 6357
Size: 1813 4544
BtgI C'CryG_G
Cuts at: 0 509 548 2156 3811 5530 6357
Size: 509 39 1608 1655 1719 827
Fragments arranged by size:
1719 1655 1608 827 509 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2187 3565 6357
Size: 2187 1378 2792
Fragments arranged by size:
2792 2187 1378
BtsI GCAGTG_nn'
Cuts at: 0 1435 2155 6357
Size: 1435 720 4202
Fragments arranged by size:
4202 1435 720
ClaI AT'CG_AT
Cuts at: 0 4271 4541 6357
Size: 4271 270 1816
Fragments arranged by size:
4271 1816 270
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2505 2517 2540 2552 6357
Size: 2505 12 23 12 3805
Fragments arranged by size:
3805 2505 23 12 12
DraIII CAC_nnn'GTG
Cuts at: 0 4378 6357
Size: 4378 1979
DrdI GACnn_nn'nnGTC
Cuts at: 0 4152 6357
Size: 4152 2205
EaeI y'GGCC_r
Cuts at: 0 538 1406 1713 2334 2816 3695 3899 4100
Size: 538 868 307 621 482 879 204 201
Cuts at: 4100 4287 4826 6123 6296 6357
Size: 187 539 1297 173 61
Fragments arranged by size:
1297 879 868 621 539 538 482 307
204 201 187 173 61
EagI C'GGCC_G
Cuts at: 0 2816 6357
Size: 2816 3541
EarI CTCTTCn'nnn_
Cuts at: 0 94 883 2503 2900 4081 4213 4526 4765
Size: 94 789 1620 397 1181 132 313 239
Cuts at: 4765 5233 5303 6032 6268 6280 6357
Size: 468 70 729 236 12 77
Fragments arranged by size:
1620 1181 789 729 468 397 313 239
236 132 94 77 70 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 285 619 665 2383 2797 3253 3259 3310
Size: 285 334 46 1718 414 456 6 51
Cuts at: 3310 4372 5194 6357
Size: 1062 822 1163
Fragments arranged by size:
1718 1163 1062 822 456 414 334 285
51 46 6
EcoICRI GAG'CTC
Cuts at: 0 1677 2199 2346 3879 4239 6357
Size: 1677 522 147 1533 360 2118
Fragments arranged by size:
2118 1677 1533 522 360 147
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 439 614 1171 1190 1264 1580 1753
Size: 404 35 175 557 19 74 316 173
Cuts at: 1753 2140 2161 2347 2380 2855 3376 4024 4348
Size: 387 21 186 33 475 521 648 324
Cuts at: 4348 4621 4642 4663 5005 5053 5134 5365 5742
Size: 273 21 21 342 48 81 231 377
Cuts at: 5742 5814 6336 6357
Size: 72 522 21
Fragments arranged by size:
648 557 522 521 475 404 387 377
342 324 316 273 231 186 175 173
81 74 72 48 35 33 21 21
21 21 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 181 1044 2122 2401 4603 5077 5793 6357
Size: 181 863 1078 279 2202 474 716 564
Fragments arranged by size:
2202 1078 863 716 564 474 279 181
EcoO109I rG'GnC_Cy
Cuts at: 0 159 258 273 344 1129 1610 1773 3135
Size: 159 99 15 71 785 481 163 1362
Cuts at: 3135 3367 3395 3396 3540 4071 4665 4755 4837
Size: 232 28 1 144 531 594 90 82
Cuts at: 4837 4896 5040 5391 5493 6165 6357
Size: 59 144 351 102 672 192
Fragments arranged by size:
1362 785 672 594 531 481 351 232
192 163 159 144 144 102 99 90
82 71 59 28 15 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 98 1353 2820 4776 5805 5883 5961 6357
Size: 98 1255 1467 1956 1029 78 78 396
Fragments arranged by size:
1956 1467 1255 1029 396 98 78 78
FseI GG_CCGG'CC
Cuts at: 0 544 6357
Size: 544 5813
FspI TGC'GCA
Cuts at: 0 2284 3190 6357
Size: 2284 906 3167
Fragments arranged by size:
3167 2284 906
HaeII r_GCGC'y
Cuts at: 0 786 1147 2591 3177 3339 4039 6177 6357
Size: 786 361 1444 586 162 700 2138 180
Fragments arranged by size:
2138 1444 786 700 586 361 180 162
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 172 204 472 504 966 998 1066 1098
Size: 172 32 268 32 462 32 68 32
Cuts at: 1098 2155 2187 3034 3066 3421 3453 3547 3579
Size: 1057 32 847 32 355 32 94 32
Cuts at: 3579 4336 4351 4368 4383 4462 4494 4654 4686
Size: 757 15 17 15 79 32 160 32
Cuts at: 4686 4713 4745 5007 5039 5059 5091 5570 5602
Size: 27 32 262 32 20 32 479 32
Cuts at: 5602 5848 5880 6357
Size: 246 32 477
Fragments arranged by size:
1057 847 757 479 477 462 355 268
262 246 172 160 94 79 68 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 27 20
17 15 15
HincII GTy'rAC
Cuts at: 0 3790 5109 5478 6357
Size: 3790 1319 369 879
Fragments arranged by size:
3790 1319 879 369
HindIII A'AGCT_T
Cuts at: 0 6157 6357
Size: 6157 200
KasI G'GCGC_C
Cuts at: 0 782 2587 3335 6173 6357
Size: 782 1805 748 2838 184
Fragments arranged by size:
2838 1805 782 748 184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 5623 6357
Size: 5623 734
MscI TGG'CCA
Cuts at: 0 4102 6125 6298 6357
Size: 4102 2023 173 59
Fragments arranged by size:
4102 2023 173 59
MslI CAynn'nnrTG
Cuts at: 0 2440 2974 3727 4009 4216 4798 5632 6357
Size: 2440 534 753 282 207 582 834 725
Fragments arranged by size:
2440 834 753 725 582 534 282 207
MspA1I CmG'CkG
Cuts at: 0 378 789 829 1944 2085 2187 2280 2475
Size: 378 411 40 1115 141 102 93 195
Cuts at: 2475 3332 3376 4098 4641 4824 5755 5955 6357
Size: 857 44 722 543 183 931 200 402
Fragments arranged by size:
1115 931 857 722 543 411 402 378
200 195 183 141 102 93 44 40
NaeI GCC'GGC
Cuts at: 0 542 1717 2820 3695 3899 4294 6357
Size: 542 1175 1103 875 204 395 2063
Fragments arranged by size:
2063 1175 1103 875 542 395 204
NarI GG'CG_CC
Cuts at: 0 783 2588 3336 6174 6357
Size: 783 1805 748 2838 183
Fragments arranged by size:
2838 1805 783 748 183
NcoI C'CATG_G
Cuts at: 0 509 6357
Size: 509 5848
NgoMIV G'CCGG_C
Cuts at: 0 540 1715 2818 3693 3897 4292 6357
Size: 540 1175 1103 875 204 395 2065
Fragments arranged by size:
2065 1175 1103 875 540 395 204
NspI r_CATG'y
Cuts at: 0 3528 5377 5913 6357
Size: 3528 1849 536 444
Fragments arranged by size:
3528 1849 536 444
PasI CC'CwG_GG
Cuts at: 0 2006 3399 4229 4833 5496 5697 6357
Size: 2006 1393 830 604 663 201 660
Fragments arranged by size:
2006 1393 830 663 660 604 201
PciI A'CATG_T
Cuts at: 0 3524 6357
Size: 3524 2833
PflMI CCAn_nnn'nTGG
Cuts at: 0 2223 2877 4378 4824 6357
Size: 2223 654 1501 446 1533
Fragments arranged by size:
2223 1533 1501 654 446
PfoI T'CCnGG_A
Cuts at: 0 614 2934 3947 5399 5588 6357
Size: 614 2320 1013 1452 189 769
Fragments arranged by size:
2320 1452 1013 769 614 189
PmlI CAC'GTG
Cuts at: 0 5409 6357
Size: 5409 948
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2635 2667 4519 4551 6357
Size: 2635 32 1852 32 1806
Fragments arranged by size:
2635 1852 1806 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 159 258 273 1773 3135 3540 4837 4896
Size: 159 99 15 1500 1362 405 1297 59
Cuts at: 4896 5040 6165 6357
Size: 144 1125 192
Fragments arranged by size:
1500 1362 1297 1125 405 192 159 144
99 59 15
PshAI GACnn'nnGTC
Cuts at: 0 2885 5104 6357
Size: 2885 2219 1253
Fragments arranged by size:
2885 2219 1253
PspOMI G'GGCC_C
Cuts at: 0 1609 3395 5346 6357
Size: 1609 1786 1951 1011
Fragments arranged by size:
1951 1786 1609 1011
PspXI vC'TCGA_Gb
Cuts at: 0 3295 6357
Size: 3295 3062
PstI C_TGCA'G
Cuts at: 0 362 834 1868 2215 2355 2450 3375 4889
Size: 362 472 1034 347 140 95 925 1514
Cuts at: 4889 5441 5468 5894 6357
Size: 552 27 426 463
Fragments arranged by size:
1514 1034 925 552 472 463 426 362
347 140 95 27
PvuII CAG'CTG
Cuts at: 0 789 2085 2187 2280 3376 4098 4641 4824
Size: 789 1296 102 93 1096 722 543 183
Cuts at: 4824 5755 5955 6357
Size: 931 200 402
Fragments arranged by size:
1296 1096 931 789 722 543 402 200
183 102 93
SacI G_AGCT'C
Cuts at: 0 1679 2201 2348 3881 4241 6357
Size: 1679 522 147 1533 360 2116
Fragments arranged by size:
2116 1679 1533 522 360 147
SalI G'TCGA_C
Cuts at: 0 5107 5476 6357
Size: 5107 369 881
Fragments arranged by size:
5107 881 369
SanDI GG'GwC_CC
Cuts at: 0 3135 4837 6357
Size: 3135 1702 1520
Fragments arranged by size:
3135 1702 1520
SbfI CC_TGCA'GG
Cuts at: 0 362 6357
Size: 362 5995
ScaI AGT'ACT
Cuts at: 0 1828 6357
Size: 1828 4529
SfcI C'TryA_G
Cuts at: 0 358 830 1864 2211 2351 2446 2545 3371
Size: 358 472 1034 347 140 95 99 826
Cuts at: 3371 4885 5427 5437 5464 5890 6357
Size: 1514 542 10 27 426 467
Fragments arranged by size:
1514 1034 826 542 472 467 426 358
347 140 99 95 27 10
SfoI GGC'GCC
Cuts at: 0 784 2589 3337 6175 6357
Size: 784 1805 748 2838 182
Fragments arranged by size:
2838 1805 784 748 182
SmaI CCC'GGG
Cuts at: 0 635 690 3538 6357
Size: 635 55 2848 2819
Fragments arranged by size:
2848 2819 635 55
SmlI C'TyrA_G
Cuts at: 0 163 3295 3388 3450 6160 6357
Size: 163 3132 93 62 2710 197
Fragments arranged by size:
3132 2710 197 163 93 62
SphI G_CATG'C
Cuts at: 0 5913 6357
Size: 5913 444
SspI AAT'ATT
Cuts at: 0 5299 6357
Size: 5299 1058
StuI AGG'CCT
Cuts at: 0 4657 6357
Size: 4657 1700
StyI C'CwwG_G
Cuts at: 0 254 509 652 2795 2999 4892 5078 5945
Size: 254 255 143 2143 204 1893 186 867
Cuts at: 5945 6080 6169 6357
Size: 135 89 188
Fragments arranged by size:
2143 1893 867 255 254 204 188 186
143 135 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2215 2269 2302 3168 5196 6357
Size: 2215 54 33 866 2028 1161
Fragments arranged by size:
2215 2028 1161 866 54 33
TatI w'GTAC_w
Cuts at: 0 1826 1961 3152 6357
Size: 1826 135 1191 3205
Fragments arranged by size:
3205 1826 1191 135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4491 4523 5259 5291 6357
Size: 4491 32 736 32 1066
Fragments arranged by size:
4491 1066 736 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 67 486 2431 6357
Size: 67 419 1945 3926
Fragments arranged by size:
3926 1945 419 67
XbaI T'CTAG_A
Cuts at: 0 721 6357
Size: 721 5636
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 589 2163 3726 3751 3818 3913 4008 4059
Size: 589 1574 1563 25 67 95 95 51
Cuts at: 4059 4323 6357
Size: 264 2034
Fragments arranged by size:
2034 1574 1563 589 264 95 95 67
51 25
XhoI C'TCGA_G
Cuts at: 0 3295 6357
Size: 3295 3062
XmaI C'CCGG_G
Cuts at: 0 633 688 3536 6357
Size: 633 55 2848 2821
Fragments arranged by size:
2848 2821 633 55
XmnI GAAnn'nnTTC
Cuts at: 0 238 1249 1921 2650 6349 6357
Size: 238 1011 672 729 3699 8
Fragments arranged by size:
3699 1011 729 672 238 8
ZraI GAC'GTC
Cuts at: 0 2772 6357
Size: 2772 3585
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AhdI AleI
AlwNI ApaI ApoI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI FseI FspI HaeII
Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI
SmaI SmlI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AlfI AloI ApaLI AscI
AseI AsiSI AvrII BcgI BciVI BclI BfrBI BmtI
BsiWI BsmI BsrGI BssHII BstAPI BstBI BstZ17I DraI
EcoRI EcoRV FspAI HpaI KpnI MfeI MluI NdeI
NheI NotI NruI NsiI PacI PmeI PsiI PsrI
PvuI RsrII SacII SapI SexAI SfiI SgfI SgrAI
SnaBI SpeI SrfI SwaI