Restriction map for
pFN21AB6546
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BalI
BanI BanII BbeI BbrPI BciVI
BclI BcnI BfaI BglI BlnI
BlpI Bpu1102I BsaAI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
Cfr13I ClaI CpoI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MaeI
MaeII MfeI MluI MluNI MroI
MscI MslI MspI MunI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SacII SalI
Sau96I SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AflII 154 CTTAAG C^TTAAG
BamHI 588 GGATCC G^GATCC
BbvCI 411 GCTGAGG CCTCAGC(-5/-2)
BcgI 289 CGAGAGGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 154 CTTAAG C^TTAAG
BglII 621 AGATCT A^GATCT
BmyI 770 GTGCCC G[AGT]GC[ACT]^C
BsaBI 589 GATCCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 403 CTCCTC GAGGAG(10/8)
BsgI 817 CTGCAC GTGCAG(16/14)
BsmFI 252 GGGAC GGGAC(10/14)
Bsp1286I 770 GTGCCC G[AGT]GC[ACT]^C
BspHI 39 TCATGA T^CATGA
BspMI 435 GCAGGT ACCTGC(4/8)
Cac8I 660 GCATGC GC[ACGT]^[ACGT]GC
DraI 450 TTTAAA TTT^AAA
EcoNI 904 CCTTTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 22 GATATC GAT^ATC
HaeIII 273 GGCC GG^CC
MamI 589 GATCCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 409 CAGCTG C[AC]G^C[GT]G
MwoI 274 GCCTATCATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 507 CCATGG C^CATGG
NdeI 131 CATATG CA^TATG
NlaIV 588 GGATCC GG[ACGT]^[ACGT]CC
PflMI 507 CCATGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 409 CAGCTG CAG^CTG
RcaI 39 TCATGA T^CATGA
SphI 660 GCATGC GCATG^C
StuI 272 AGGCCT AGG^CCT
SwaI 449 ATTTAAAT ATTT^AAAT
Van91I 507 CCATGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AfaI 715 GTAC GT^AC
989 GTAC
BbvI 378 GCAGC GCAGC(8/12)
657 GCAGC
BpmI 71 CTCCAG CTGGAG(16/14)
406 CTCCAG
BsmAI 803 GAGAC GTCTC(1/5)
1075 GAGAC
BssKI 910 CCAGG ^CC[ACGT]GG
1043 CCTGG
BstNI 910 CCAGG CC^[AT]GG
1043 CCTGG
DsaI 248 CCGTGG C^C[GA][CT]GG
507 CCATGG
EcoRII 910 CCAGG ^CC[AT]GG
1043 CCTGG
EcoT14I 507 CCATGG C^C[AT][AT]GG
795 CCTTGG
EcoT22I 281 ATGCAT ATGCA^T
662 ATGCAT
Fnu4HI 378 GCAGC GC^[ACGT]GC
657 GCAGC
HphI 438 GGTGA GGTGA(8/7)
705 GGTGA
ItaI 378 GCAGC GC^[ACGT]GC
657 GCAGC
MvaI 910 CCAGG CC^[AT]GG
1043 CCTGG
NsiI 281 ATGCAT ATGCA^T
662 ATGCAT
NspI 660 GCATGC [GA]CATG^[CT]
991 ACATGC
PleI 69 GACTC GAGTC(4/5)
708 GACTC
RsaI 715 GTAC GT^AC
989 GTAC
SapI 318 GAAGAGC GCTCTTC(1/4)
612 GAAGAGC
ScrFI 910 CCAGG CC^[ACGT]GG
1043 CCTGG
SfaNI 4 GATGC GCATC(5/9)
782 GATGC
SfcI 568 CTACAG C^T[GA][CT]AG
776 CTGTAG
SmlI 154 CTTAAG C^T[CT][GA]AG
710 CTCAAG
StyI 507 CCATGG C^C[AT][AT]GG
795 CCTTGG
TaqI 225 TCGA T^CGA
288 TCGA
TseI 378 GCAGC G^C[AT]GC
657 GCAGC
TthHB8I 225 TCGA T^CGA
288 TCGA
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbsI
BpuAI BsaJI BsiYI BslI BsrI
BstF5I BstYI DdeI DpnII EarI
FokI HinfI Ksp632I MaeIII MboI
MboII MflI MnlI MseI NdeII
NlaIII Sau3AI TfiI Tru9I Tsp45I
Tsp509I TspRI XhoII