(Circular) (Six-base) MAPSORT of: pFN21AB6546.seq Check: 5974 from: 1 to: 5881 pFN21AB6546 5881 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2431 2431 Size: 5881 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3691 278 Size: 53 83 186 936 2155 2468 Fragments arranged by size: 2468 2155 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3224 1937 Size: 1287 4594 Acc65I G'GTAC_C Cuts at: 3202 3202 Size: 5881 AclI AA'CG_TT Cuts at: 1563 4008 4381 1563 Size: 2445 373 3063 Fragments arranged by size: 3063 2445 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2525 2636 2702 3944 5328 5596 1632 Size: 893 111 66 1242 1384 268 1917 Fragments arranged by size: 1917 1384 1242 893 268 111 66 AflII C'TTAA_G Cuts at: 820 1017 2159 820 Size: 197 1142 4542 Fragments arranged by size: 4542 1142 197 AflIII A'CryG_T Cuts at: 1203 4780 5757 1203 Size: 3577 977 1327 Fragments arranged by size: 3577 1327 977 AgeI A'CCGG_T Cuts at: 4712 4712 Size: 5881 AhdI GACnn_n'nnGTC Cuts at: 4610 4610 Size: 5881 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5881 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2837 1184 Size: 1653 4228 AlwNI CAG_nnn'CTG Cuts at: 3624 5196 3624 Size: 1572 4309 ApaLI G'TGCA_C Cuts at: 3938 5094 3938 Size: 1156 4725 ApoI r'AATT_y Cuts at: 1493 2452 2833 3028 3190 3416 3568 5677 1493 Size: 959 381 195 162 226 152 2109 1697 Fragments arranged by size: 2109 1697 959 381 226 195 162 152 AseI AT'TA_AT Cuts at: 160 1032 4435 160 Size: 872 3403 1606 Fragments arranged by size: 3403 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5881 AvaI C'yCGr_G Cuts at: 1870 1943 3206 1870 Size: 73 1263 4545 Fragments arranged by size: 4545 1263 73 BamHI G'GATC_C Cuts at: 2593 3211 3578 2593 Size: 618 367 4896 Fragments arranged by size: 4896 618 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3202 4657 618 Size: 325 217 627 1415 1455 1842 Fragments arranged by size: 1842 1455 1415 627 325 217 BanII G_rGCy'C Cuts at: 1391 3200 1391 Size: 1809 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2148 2613 2802 3045 928 Size: 971 249 465 189 243 3764 Fragments arranged by size: 3764 971 465 249 243 189 BbvCI CC'TCA_GC Cuts at: 2417 2417 Size: 5881 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2283 2317 4072 4106 1903 Size: 34 346 34 1755 34 3678 Fragments arranged by size: 3678 1755 346 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3773 4983 3773 Size: 1210 4671 BclI T'GATC_A Cuts at: 1548 1548 Size: 5881 BfrBI ATG'CAT Cuts at: 2288 2669 2288 Size: 381 5500 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4492 136 Size: 107 122 71 631 605 2820 1525 Fragments arranged by size: 2820 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2626 5876 2626 Size: 3250 2631 BlpI GC'TnA_GC Cuts at: 3298 3298 Size: 5881 Bme1580I G_kGCm'C Cuts at: 1790 2779 3942 5098 1790 Size: 989 1163 1156 2573 Fragments arranged by size: 2573 1163 1156 989 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5881 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2485 2683 3612 Size: 416 53 83 399 24 1068 198 929 Cuts at: 3612 4570 442 Size: 958 1753 Fragments arranged by size: 1753 1068 958 929 416 399 198 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5881 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2061 2396 4541 1239 Size: 822 335 2145 2579 Fragments arranged by size: 2579 2145 822 335 Bpu10I CC'TnA_GC Cuts at: 2417 2417 Size: 5881 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2700 4006 4871 5169 2700 Size: 1306 865 298 3412 Fragments arranged by size: 3412 1306 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4544 882 Size: 617 3045 2219 Fragments arranged by size: 3045 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3672 493 Size: 711 2468 2702 Fragments arranged by size: 2702 2468 711 BsaBI GATnn'nnATC Cuts at: 2598 5875 2598 Size: 3277 2604 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3688 4070 4705 275 Size: 53 83 186 936 2155 382 635 1451 Fragments arranged by size: 2155 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4314 4712 4986 5133 5544 4314 Size: 398 274 147 411 4651 Fragments arranged by size: 4651 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2059 2089 1178 Size: 30 165 30 656 30 4970 Fragments arranged by size: 4970 656 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2399 1186 Size: 1213 4668 BseYI C'CCAG_C Cuts at: 5084 5580 5084 Size: 496 5385 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2807 1163 Size: 573 1071 4237 Fragments arranged by size: 4237 1071 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3331 4092 5120 1540 Size: 275 186 1330 761 1028 2301 Fragments arranged by size: 2301 1330 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3942 4027 5098 3942 Size: 85 1071 4725 Fragments arranged by size: 4725 1071 85 BsmI GAATG_Cn' Cuts at: 3392 3485 3392 Size: 93 5788 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2779 3200 3942 4027 5098 1391 Size: 399 989 421 742 85 1071 2174 Fragments arranged by size: 2174 1071 989 742 421 399 85 BspEI T'CCGG_A Cuts at: 5544 5544 Size: 5881 BspHI T'CATG_A Cuts at: 2044 3770 5500 2044 Size: 1726 1730 2425 Fragments arranged by size: 2425 1730 1726 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2431 3236 844 Size: 1587 805 3489 Fragments arranged by size: 3489 1587 805 BsrBI CCG'CTC Cuts at: 1394 1823 3768 1394 Size: 429 1945 3507 Fragments arranged by size: 3507 1945 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4376 4550 65 Size: 1183 3128 174 1396 Fragments arranged by size: 3128 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4525 4712 1700 Size: 110 2715 187 2869 Fragments arranged by size: 2869 2715 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5881 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5881 BssSI C'ACGA_G Cuts at: 3941 4953 3941 Size: 1012 4869 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5881 BstBI TT'CG_AA Cuts at: 4685 4685 Size: 5881 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5881 BstYI r'GATC_y Cuts at: 1972 2508 2593 2626 2975 3211 3578 3963 3980 Size: 536 85 33 349 236 367 385 17 Cuts at: 3980 5432 5518 5530 5876 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 536 385 367 349 346 236 86 85 33 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2253 2512 513 Size: 552 939 249 259 3882 Fragments arranged by size: 3882 939 552 259 249 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5142 Fragments arranged by size: 5142 683 56 BtsI GCAGTG_nn' Cuts at: 2130 2797 3134 3397 4191 4211 2130 Size: 667 337 263 794 20 3800 Fragments arranged by size: 3800 794 667 337 263 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5881 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2235 2270 529 Size: 35 1671 35 4140 Fragments arranged by size: 4140 1671 35 35 DraI TTT'AAA Cuts at: 2457 3185 2457 Size: 728 5153 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4888 809 Size: 507 3572 1802 Fragments arranged by size: 3572 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3328 4217 5846 Size: 54 1425 50 275 18 1498 889 1629 Cuts at: 5846 8 Size: 43 Fragments arranged by size: 1629 1498 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3328 1537 Size: 275 1516 4090 Fragments arranged by size: 4090 1516 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2189 2318 2612 2678 2800 3811 1353 Size: 836 129 294 66 122 1011 3423 Fragments arranged by size: 3423 1011 836 294 129 122 66 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4468 4842 4988 387 Size: 1064 138 2879 374 146 1280 Fragments arranged by size: 2879 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2061 2396 2525 2636 2702 3944 4541 Size: 393 429 335 129 111 66 1242 597 Cuts at: 4541 5328 5596 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1242 787 597 429 393 335 268 129 111 66 EcoNI CCTnn'n_nnAGG Cuts at: 2913 2913 Size: 5881 EcoRI G'AATT_C Cuts at: 3190 3568 3190 Size: 378 5503 EcoRV GAT'ATC Cuts at: 2029 2029 Size: 5881 FspI TGC'GCA Cuts at: 4387 4387 Size: 5881 HaeII r_GCGC'y Cuts at: 1678 5028 1678 Size: 3350 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2611 2643 4522 4554 4596 Size: 32 135 32 1039 32 1879 32 42 Cuts at: 4596 4628 1373 Size: 32 2626 Fragments arranged by size: 2626 1879 1039 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3225 3471 669 Size: 988 281 1287 246 3079 Fragments arranged by size: 3079 1287 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5881 HpaI GTT'AAC Cuts at: 3471 3471 Size: 5881 KpnI G_GTAC'C Cuts at: 3206 3206 Size: 5881 MfeI C'AATT_G Cuts at: 3480 3480 Size: 5881 MluI A'CGCG_T Cuts at: 5757 5757 Size: 5881 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4995 5179 1305 Size: 3690 184 2007 Fragments arranged by size: 3690 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4059 Fragments arranged by size: 4059 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3839 4198 4357 518 Size: 648 2673 359 159 2042 Fragments arranged by size: 2673 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2416 3297 3974 5122 5367 1606 Size: 810 881 677 1148 245 2120 Fragments arranged by size: 2120 1148 881 810 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2512 513 Size: 552 939 508 3882 Fragments arranged by size: 3882 939 552 508 NdeI CA'TA_TG Cuts at: 387 2137 387 Size: 1750 4131 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5881 NotI GC'GGCC_GC Cuts at: 3328 3328 Size: 5881 NsiI A_TGCA'T Cuts at: 2290 2671 2290 Size: 381 5500 NspI r_CATG'y Cuts at: 1132 1229 2669 3000 3239 4784 1132 Size: 97 1440 331 239 1545 2229 Fragments arranged by size: 2229 1545 1440 331 239 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5881 PciI A'CATG_T Cuts at: 4780 4780 Size: 5881 PflMI CCAn_nnn'nTGG Cuts at: 1108 2518 1108 Size: 1410 4471 PmeI GTTT'AAAC Cuts at: 3185 3185 Size: 5881 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3950 3982 5463 5495 3950 Size: 32 1481 32 4336 Fragments arranged by size: 4336 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5881 PsiI TTA'TAA Cuts at: 3174 3451 3174 Size: 277 5604 PstI C_TGCA'G Cuts at: 830 3233 830 Size: 2403 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5881 PvuII CAG'CTG Cuts at: 2416 2416 Size: 5881 SalI G'TCGA_C Cuts at: 1936 3223 1936 Size: 1287 4594 SapI GCTCTTCn'nnn_ Cuts at: 2318 2612 2318 Size: 294 5587 SbfI CC_TGCA'GG Cuts at: 3233 3233 Size: 5881 ScaI AGT'ACT Cuts at: 4129 5841 4129 Size: 1712 4169 SfcI C'TryA_G Cuts at: 826 1045 2573 2781 3229 4364 5045 5236 826 Size: 219 1528 208 448 1135 681 191 1471 Fragments arranged by size: 1528 1471 1135 681 448 219 208 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5881 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5881 SmaI CCC'GGG Cuts at: 1872 3208 1872 Size: 1336 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2159 2715 3985 4886 5148 820 Size: 197 926 216 556 1270 901 262 1553 Fragments arranged by size: 1553 1270 926 901 556 262 216 197 SnaBI TAC'GTA Cuts at: 493 3672 493 Size: 3179 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5881 SphI G_CATG'C Cuts at: 1132 2669 3239 1132 Size: 1537 570 3774 Fragments arranged by size: 3774 1537 570 SspI AAT'ATT Cuts at: 5 52 3805 5 Size: 47 3753 2081 Fragments arranged by size: 3753 2081 47 StuI AGG'CCT Cuts at: 1517 2279 1517 Size: 762 5119 StyI C'CwwG_G Cuts at: 513 1065 2004 2512 2800 3320 513 Size: 552 939 508 288 520 3074 Fragments arranged by size: 3074 939 552 520 508 288 SwaI ATTT'AAAT Cuts at: 2457 2457 Size: 5881 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3908 3925 4078 4263 4708 325 Size: 1255 2328 17 153 185 445 1498 Fragments arranged by size: 2328 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2719 4127 5839 Size: 274 80 33 51 1443 742 1408 1712 Cuts at: 5839 96 Size: 138 Fragments arranged by size: 1712 1443 1408 742 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2842 2874 5841 5873 2842 Size: 32 2967 32 2850 Fragments arranged by size: 2967 2850 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5881 XbaI T'CTAG_A Cuts at: 3217 3217 Size: 5881 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5881 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5881 XmaI C'CCGG_G Cuts at: 1870 3206 1870 Size: 1336 4545 XmnI GAAnn'nnTTC Cuts at: 4010 4010 Size: 5881 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3689 276 Size: 53 83 186 936 2155 2468 Fragments arranged by size: 2468 2155 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI