(Circular) (Six-base) MAPSORT of: pFN21AB9466.seq Check: 7730 from: 1 to: 7036 pFN21AB9466 7036 bp With 182 enzymes: SgfI * May 6, 2010 17:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4846 278 Size: 53 83 186 936 3310 2468 Fragments arranged by size: 3310 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2399 2555 4379 1937 Size: 462 156 1824 4594 Fragments arranged by size: 4594 1824 462 156 Acc65I G'GTAC_C Cuts at: 4357 4357 Size: 7036 AclI AA'CG_TT Cuts at: 1563 3575 5163 5536 1563 Size: 2012 1588 373 3063 Fragments arranged by size: 3063 2012 1588 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2481 3273 3434 3648 3956 4008 4064 5099 Size: 849 792 161 214 308 52 56 1035 Cuts at: 5099 6483 6751 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1035 849 792 308 268 214 161 56 52 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6839 AflIII A'CryG_T Cuts at: 1203 3696 5935 6912 1203 Size: 2493 2239 977 1327 Fragments arranged by size: 2493 2239 1327 977 AgeI A'CCGG_T Cuts at: 5867 5867 Size: 7036 AhdI GACnn_n'nnGTC Cuts at: 5765 5765 Size: 7036 AleI CACnn'nnGTG Cuts at: 1166 2441 1166 Size: 1275 5761 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3735 1184 Size: 2551 4485 AlwNI CAG_nnn'CTG Cuts at: 4779 6351 4779 Size: 1572 5464 ApaLI G'TGCA_C Cuts at: 5093 6249 5093 Size: 1156 5880 ApoI r'AATT_y Cuts at: 1493 3405 3501 4247 4345 4571 4723 6832 1493 Size: 1912 96 746 98 226 152 2109 1697 Fragments arranged by size: 2109 1912 1697 746 226 152 98 96 AseI AT'TA_AT Cuts at: 160 1032 3891 5590 160 Size: 872 2859 1699 1606 Fragments arranged by size: 2859 1699 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7036 AvaI C'yCGr_G Cuts at: 1870 1943 2344 4253 4361 1870 Size: 73 401 1909 108 4545 Fragments arranged by size: 4545 1909 401 108 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2981 3014 2981 Size: 33 7003 BamHI G'GATC_C Cuts at: 4366 4733 4366 Size: 367 6669 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2528 3102 4357 5812 618 Size: 325 217 627 741 574 1255 1455 1842 Fragments arranged by size: 1842 1455 1255 741 627 574 325 217 BanII G_rGCy'C Cuts at: 1391 4355 1391 Size: 2964 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2127 2188 2703 3440 928 Size: 971 228 61 515 737 4524 Fragments arranged by size: 4524 971 737 515 228 61 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5227 5261 1903 Size: 34 3290 34 3678 Fragments arranged by size: 3678 3290 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4928 6138 4928 Size: 1210 5826 BclI T'GATC_A Cuts at: 1548 1548 Size: 7036 BfrBI ATG'CAT Cuts at: 4180 4180 Size: 7036 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2526 5647 136 Size: 107 122 71 631 605 854 3121 1525 Fragments arranged by size: 3121 1525 854 631 605 122 107 71 BglII A'GATC_T Cuts at: 2233 2788 7031 2233 Size: 555 4243 2238 Fragments arranged by size: 4243 2238 555 BlpI GC'TnA_GC Cuts at: 4453 4453 Size: 7036 Bme1580I G_kGCm'C Cuts at: 1790 2900 5097 6253 1790 Size: 1110 2197 1156 2573 Fragments arranged by size: 2573 2197 1156 1110 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7036 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2802 2903 4767 Size: 416 53 83 399 24 1385 101 1864 Cuts at: 4767 5725 442 Size: 958 1753 Fragments arranged by size: 1864 1753 1385 958 416 399 101 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7036 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3924 3924 Size: 7036 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2946 3006 3135 4111 5696 1239 Size: 1707 60 129 976 1585 2579 Fragments arranged by size: 2579 1707 1585 976 129 60 Bpu10I CC'TnA_GC Cuts at: 2064 2064 Size: 7036 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5161 6026 6324 5161 Size: 865 298 5873 Fragments arranged by size: 5873 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3193 5699 882 Size: 617 1694 2506 2219 Fragments arranged by size: 2506 2219 1694 617 BsaAI yAC'GTr Cuts at: 493 1204 4827 493 Size: 711 3623 2702 Fragments arranged by size: 3623 2702 711 BsaBI GATnn'nnATC Cuts at: 7030 7030 Size: 7036 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4843 5225 5860 275 Size: 53 83 186 936 3310 382 635 1451 Fragments arranged by size: 3310 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2755 5469 5867 6141 6288 6699 2755 Size: 2714 398 274 147 411 3092 Fragments arranged by size: 3092 2714 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2103 2133 2778 2808 1178 Size: 30 165 30 700 30 645 30 5406 Fragments arranged by size: 5406 700 645 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2121 2124 2673 3207 3220 1186 Size: 935 3 549 534 13 5002 Fragments arranged by size: 5002 935 549 534 13 3 BseYI C'CCAG_C Cuts at: 2048 3191 3424 6239 6735 2048 Size: 1143 233 2815 496 2349 Fragments arranged by size: 2815 2349 1143 496 233 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2848 1163 Size: 573 1112 5351 Fragments arranged by size: 5351 1112 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4486 5247 6275 1540 Size: 275 186 2485 761 1028 2301 Fragments arranged by size: 2485 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3278 5097 5182 6253 3278 Size: 1819 85 1071 4061 Fragments arranged by size: 4061 1819 1071 85 BsmI GAATG_Cn' Cuts at: 4547 4640 4547 Size: 93 6943 BsmBI CGTCTCn'nnnn_ Cuts at: 2618 2618 Size: 7036 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2900 3278 4355 5097 5182 6253 1391 Size: 399 1110 378 1077 742 85 1071 2174 Fragments arranged by size: 2174 1110 1077 1071 742 399 378 85 BspEI T'CCGG_A Cuts at: 6699 6699 Size: 7036 BspHI T'CATG_A Cuts at: 3681 4925 6655 3681 Size: 1244 1730 4062 Fragments arranged by size: 4062 1730 1244 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3870 4391 844 Size: 3026 521 3489 Fragments arranged by size: 3489 3026 521 BsrBI CCG'CTC Cuts at: 1394 1823 4923 1394 Size: 429 3100 3507 Fragments arranged by size: 3507 3100 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2224 2379 2548 5531 5705 65 Size: 1183 976 155 169 2983 174 1396 Fragments arranged by size: 2983 1396 1183 976 174 169 155 BsrFI r'CCGG_y Cuts at: 1700 1810 5680 5867 1700 Size: 110 3870 187 2869 Fragments arranged by size: 3870 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7036 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7036 BssSI C'ACGA_G Cuts at: 5096 6108 5096 Size: 1012 6024 BstAPI GCAn_nnn'nTGC Cuts at: 794 2438 794 Size: 1644 5392 BstBI TT'CG_AA Cuts at: 3409 5840 3409 Size: 2431 4605 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7036 BstXI CCAn_nnnn'nTGG Cuts at: 3113 3113 Size: 7036 BstYI r'GATC_y Cuts at: 1972 2233 2788 3110 4366 4733 5118 5135 6587 Size: 261 555 322 1256 367 385 17 1452 Cuts at: 6587 6673 6685 7031 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1256 555 385 367 346 322 261 86 17 12 BstZ17I GTA'TAC Cuts at: 2556 2556 Size: 7036 Bsu36I CC'TnA_GG Cuts at: 4032 4032 Size: 7036 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5545 Fragments arranged by size: 5545 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6297 Fragments arranged by size: 6297 683 56 BtsI GCAGTG_nn' Cuts at: 2059 2436 2819 3354 3732 4552 5346 5366 2059 Size: 377 383 535 378 820 794 20 3729 Fragments arranged by size: 3729 820 794 535 383 378 377 20 ClaI AT'CG_AT Cuts at: 1554 3399 1554 Size: 1845 5191 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7001 DraI TTT'AAA Cuts at: 4340 4340 Size: 7036 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6043 809 Size: 507 4727 1802 Fragments arranged by size: 4727 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4483 5372 7001 Size: 54 1425 50 275 18 2653 889 1629 Cuts at: 7001 8 Size: 43 Fragments arranged by size: 2653 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4483 1537 Size: 275 2671 4090 Fragments arranged by size: 4090 2671 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3981 4027 4040 4966 1353 Size: 2628 46 13 926 3423 Fragments arranged by size: 3423 2628 926 46 13 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5623 5997 6143 387 Size: 1064 138 4034 374 146 1280 Fragments arranged by size: 4034 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2481 2946 3006 3135 3273 3434 3648 Size: 393 849 465 60 129 138 161 214 Cuts at: 3648 3956 4008 4064 4111 5099 5696 6483 6751 Size: 308 52 56 47 988 597 787 268 Cuts at: 6751 1239 Size: 1524 Fragments arranged by size: 1524 988 849 787 597 465 393 308 268 214 161 138 129 60 56 52 47 EcoO109I rG'GnC_Cy Cuts at: 2610 2610 Size: 7036 EcoRI G'AATT_C Cuts at: 3501 4247 4345 4723 3501 Size: 746 98 378 5814 Fragments arranged by size: 5814 746 378 98 FspI TGC'GCA Cuts at: 2223 5542 2223 Size: 3319 3717 HaeII r_GCGC'y Cuts at: 1678 6183 1678 Size: 4505 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3197 3229 5677 5709 5751 Size: 32 135 32 1625 32 2448 32 42 Cuts at: 5751 5783 1373 Size: 32 2626 Fragments arranged by size: 2626 2448 1625 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2400 4380 4626 669 Size: 988 281 462 1980 246 3079 Fragments arranged by size: 3079 1980 988 462 281 246 HindIII A'AGCT_T Cuts at: 748 2363 2917 3285 748 Size: 1615 554 368 4499 Fragments arranged by size: 4499 1615 554 368 HpaI GTT'AAC Cuts at: 4626 4626 Size: 7036 KpnI G_GTAC'C Cuts at: 4361 4361 Size: 7036 MfeI C'AATT_G Cuts at: 4635 4635 Size: 7036 MluI A'CGCG_T Cuts at: 6912 6912 Size: 7036 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3591 6150 6334 1305 Size: 2286 2559 184 2007 Fragments arranged by size: 2559 2286 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5214 Fragments arranged by size: 5214 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2441 2487 3771 4994 5353 5512 518 Size: 648 1275 46 1284 1223 359 159 2042 Fragments arranged by size: 2042 1284 1275 1223 648 359 159 46 MspA1I CmG'CkG Cuts at: 1606 4144 4452 5129 6277 6522 1606 Size: 2538 308 677 1148 245 2120 Fragments arranged by size: 2538 2120 1148 677 308 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5545 Fragments arranged by size: 5545 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7036 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7036 NotI GC'GGCC_GC Cuts at: 4483 4483 Size: 7036 NsiI A_TGCA'T Cuts at: 4182 4182 Size: 7036 NspI r_CATG'y Cuts at: 1132 1229 3432 3700 3922 3943 4394 5939 1132 Size: 97 2203 268 222 21 451 1545 2229 Fragments arranged by size: 2229 2203 1545 451 268 222 97 21 PasI CC'CwG_GG Cuts at: 1348 2870 1348 Size: 1522 5514 PciI A'CATG_T Cuts at: 3696 5935 3696 Size: 2239 4797 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7036 PfoI T'CCnGG_A Cuts at: 2923 3800 2923 Size: 877 6159 PmeI GTTT'AAAC Cuts at: 4340 4340 Size: 7036 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5105 5137 6618 6650 5105 Size: 32 1481 32 5491 Fragments arranged by size: 5491 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2610 2610 Size: 7036 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7036 PsiI TTA'TAA Cuts at: 4606 4606 Size: 7036 PstI C_TGCA'G Cuts at: 830 3795 4388 830 Size: 2965 593 3478 Fragments arranged by size: 3478 2965 593 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7036 SalI G'TCGA_C Cuts at: 1936 2398 4378 1936 Size: 462 1980 4594 Fragments arranged by size: 4594 1980 462 SanDI GG'GwC_CC Cuts at: 2610 2610 Size: 7036 SapI GCTCTTCn'nnn_ Cuts at: 4040 4040 Size: 7036 SbfI CC_TGCA'GG Cuts at: 4388 4388 Size: 7036 ScaI AGT'ACT Cuts at: 3797 5284 6996 3797 Size: 1487 1712 3837 Fragments arranged by size: 3837 1712 1487 SexAI A'CCwGG_T Cuts at: 3748 3748 Size: 7036 SfcI C'TryA_G Cuts at: 826 1045 3516 3791 4384 5519 6200 6391 826 Size: 219 2471 275 593 1135 681 191 1471 Fragments arranged by size: 2471 1471 1135 681 593 275 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7036 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7036 SmaI CCC'GGG Cuts at: 1872 4363 1872 Size: 2491 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5140 6041 6303 820 Size: 197 926 3197 901 262 1553 Fragments arranged by size: 3197 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4827 493 Size: 4334 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7036 SphI G_CATG'C Cuts at: 1132 3432 4394 1132 Size: 2300 962 3774 Fragments arranged by size: 3774 2300 962 SspI AAT'ATT Cuts at: 5 52 4295 4960 5 Size: 47 4243 665 2081 Fragments arranged by size: 4243 2081 665 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7036 StyI C'CwwG_G Cuts at: 513 1065 2004 2639 3106 4475 513 Size: 552 939 635 467 1369 3074 Fragments arranged by size: 3074 1369 939 635 552 467 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5063 5080 5233 5418 5863 325 Size: 1255 3483 17 153 185 445 1498 Fragments arranged by size: 3483 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3795 5282 6994 Size: 274 80 33 51 1443 1818 1487 1712 Cuts at: 6994 96 Size: 138 Fragments arranged by size: 1818 1712 1487 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2103 2135 6996 7028 2103 Size: 32 4861 32 2111 Fragments arranged by size: 4861 2111 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7036 XbaI T'CTAG_A Cuts at: 4372 4372 Size: 7036 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3265 3669 3265 Size: 404 6632 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7036 XmaI C'CCGG_G Cuts at: 1870 4361 1870 Size: 2491 4545 XmnI GAAnn'nnTTC Cuts at: 5165 5165 Size: 7036 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4844 276 Size: 53 83 186 936 3310 2468 Fragments arranged by size: 3310 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BsiWI DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SfiI SfoI SrfI SwaI