Restriction map for
pFN21AB9389
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BseAI BseRI
BsgI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrGI BssHII BssSI BstAPI BstBI
BstEII BstPI BstUI BstXI Bsu36I
CelII CfoI ClaI CpoI DrdI
EaeI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
HhaI HinP1I HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MslI MunI MvnI
NarI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflFI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcsI 1546 AAATTT [GA]^AATT[CT]
AcyI 658 GACGCC G[GA]^CG[CT]C
AfaI 975 GTAC GT^AC
AgeI 1091 ACCGGT A^CCGGT
AhdI 658 GACGCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 1546 AAATTT [GA]^AATT[CT]
AspEI 658 GACGCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 877 GAGCTC G[AT]GC[AT]^C
AvaI 832 CTCGAG C^[CT]CG[GA]G
BanI 856 GGCACC G^G[CT][GA]CC
BciVI 810 GGATAC GTATCC(6/5)
BclI 822 TGATCA T^GATCA
BglII 1929 AGATCT A^GATCT
BsaHI 658 GACGCC G[GA]^CG[CT]C
BsiHKAI 877 GAGCTC G[AT]GC[AT]^C
BsoBI 832 CTCGAG C^[CT]CG[GA]G
BspHI 141 TCATGA T^CATGA
BspMI 1890 ACCTGC ACCTGC(4/8)
BsrBI 888 CCGCTC CCGCTC(-3/-3)
DraI 738 TTTAAA TTT^AAA
DraII 495 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1628 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 992 CCACGG C^C[GA][CT]GG
Eam1105I 658 GACGCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1411 GAAGAG CTCTTC(1/4)
EcoO109I 495 AGGACCT [GA]G^G[ACGT]CC[CT]
FbaI 822 TGATCA T^GATCA
HgaI 658 GACGC GACGC(5/10)
Hin1I 658 GACGCC G[GA]^CG[CT]C
HincII 120 GTCAAC GT[CT]^[GA]AC
HindII 120 GTCAAC GT[CT]^[GA]AC
Ksp632I 1411 GAAGAG CTCTTC(1/4)
MaeII 526 ACGT A^CGT
NaeI 1224 GCCGGC GCC^GGC
NgoMIV 1224 GCCGGC G^CCGGC
PaeR7I 832 CTCGAG C^TCGAG
PinAI 1091 ACCGGT A^CCGGT
PleI 1453 GAGTC GAGTC(4/5)
PpuMI 495 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1936 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1892 CTGCAG CTGCA^G
RcaI 141 TCATGA T^CATGA
RsaI 975 GTAC GT^AC
SacI 877 GAGCTC GAGCT^C
SmlI 832 CTCGAG C^T[CT][GA]AG
XhoI 832 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 528 GTATAC GT^[AC][GT]AC
1202 GTATAC
AciI 888 CCGC CCGC(-3/-1)
1151 CCGC
AlwI 550 GATCC GGATC(4/5)
1300 GATCC
Asp700I 1384 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1462 GAACAGCTTC
BanII 877 GAGCTC G[GA]GC[CT]^C
1622 GAGCCC
BbsI 1354 GAAGAC GAAGAC(2/6)
1483 GAAGAC
BcnI 370 CCGGG CC^[GC]GG
860 CCCGG
BfaI 934 CTAG C^TAG
1693 CTAG
BpmI 1157 CTCCAG CTGGAG(16/14)
1619 CTGGAG
BpuAI 1354 GAAGAC GAAGAC(2/6)
1483 GAAGAC
BsaWI 429 ACCGGA [AT]^CCGG[AT]
1091 ACCGGT
BsmAI 1772 GAGAC GTCTC(1/5)
1182 GTCTC
BsmFI 757 GGGAC GGGAC(10/14)
1943 GTCCC
BsrFI 1091 ACCGGT [GA]^CCGG[CT]
1224 GCCGGC
Bst1107I 528 GTATAC GTA^TAC
1202 GTATAC
BstNI 1281 CCTGG CC^[AT]GG
1824 CCTGG
BstZ17I 528 GTATAC GTA^TAC
1202 GTATAC
Cfr10I 1091 ACCGGT [GA]^CCGG[CT]
1224 GCCGGC
EcoRII 1281 CCTGG ^CC[AT]GG
1824 CCTGG
HindIII 1366 AAGCTT A^AGCTT
1777 AAGCTT
MaeI 934 CTAG C^TAG
1693 CTAG
MvaI 1281 CCTGG CC^[AT]GG
1824 CCTGG
NciI 370 CCGGG CC^[GC]GG
860 CCCGG
NlaIV 856 GGCACC GG[ACGT]^[ACGT]CC
1621 GGAGCC
PflMI 36 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1303 CCAGAAGATGG
Van91I 36 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1303 CCAGAAGATGG
XcmI 125 CCAAGCTGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1574 CCATCTCTGAAGTGG
XmnI 1384 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1462 GAACAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AluI AlwNI AvaII BbvI BmyI
BsaJI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstYI Cac8I Cfr13I
DdeI DpnII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MwoI
NdeII NlaIII PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII