(Circular) (Six-base) MAPSORT of: pFN21AB9389.seq Check: 7893 from: 1 to: 6790 pFN21AB9389 6790 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4600 278 Size: 53 83 186 936 3064 2468 Fragments arranged by size: 3064 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2534 3208 4133 1937 Size: 597 674 925 4594 Fragments arranged by size: 4594 925 674 597 Acc65I G'GTAC_C Cuts at: 4111 4111 Size: 6790 AclI AA'CG_TT Cuts at: 1563 4917 5290 1563 Size: 3354 373 3063 Fragments arranged by size: 3354 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2526 2535 3054 3606 4853 6237 6505 1632 Size: 894 9 519 552 1247 1384 268 1917 Fragments arranged by size: 1917 1384 1247 894 552 519 268 9 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6593 AflIII A'CryG_T Cuts at: 1203 5689 6666 1203 Size: 4486 977 1327 Fragments arranged by size: 4486 1327 977 AgeI A'CCGG_T Cuts at: 3096 5621 3096 Size: 2525 4265 AhdI GACnn_n'nnGTC Cuts at: 2668 5519 2668 Size: 2851 3939 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6790 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6790 AlwNI CAG_nnn'CTG Cuts at: 2108 2705 2879 3032 3170 4533 6105 2108 Size: 597 174 153 138 1363 1572 2793 Fragments arranged by size: 2793 1572 1363 597 174 153 138 ApaLI G'TGCA_C Cuts at: 4847 6003 4847 Size: 1156 5634 ApoI r'AATT_y Cuts at: 1493 3551 4099 4325 4477 6586 1493 Size: 2058 548 226 152 2109 1697 Fragments arranged by size: 2109 2058 1697 548 226 152 AseI AT'TA_AT Cuts at: 160 1032 5344 160 Size: 872 4312 1606 Fragments arranged by size: 4312 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6790 AvaI C'yCGr_G Cuts at: 1870 1943 2837 4115 1870 Size: 73 894 1278 4545 Fragments arranged by size: 4545 1278 894 73 BamHI G'GATC_C Cuts at: 4120 4487 4120 Size: 367 6423 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2861 4111 5566 618 Size: 325 217 627 1074 1250 1455 1842 Fragments arranged by size: 1842 1455 1250 1074 627 325 217 BanII G_rGCy'C Cuts at: 1391 2886 3631 4109 1391 Size: 1495 745 478 4072 Fragments arranged by size: 4072 1495 745 478 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3366 3495 928 Size: 971 1467 129 4223 Fragments arranged by size: 4223 1467 971 129 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4981 5015 1903 Size: 34 3044 34 3678 Fragments arranged by size: 3678 3044 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2809 4682 5892 2809 Size: 1873 1210 3707 Fragments arranged by size: 3707 1873 1210 BclI T'GATC_A Cuts at: 1548 2827 1548 Size: 1279 5511 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5401 136 Size: 107 122 71 631 605 3729 1525 Fragments arranged by size: 3729 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3934 6785 3934 Size: 2851 3939 BlpI GC'TnA_GC Cuts at: 4207 4207 Size: 6790 Bme1580I G_kGCm'C Cuts at: 1790 2864 3248 4851 6007 1790 Size: 1074 384 1603 1156 2573 Fragments arranged by size: 2573 1603 1156 1074 384 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6790 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2867 4521 5479 Size: 416 53 83 399 24 1450 1654 958 Cuts at: 5479 442 Size: 1753 Fragments arranged by size: 1753 1654 1450 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6790 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3538 3538 Size: 6790 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3147 3645 5450 1239 Size: 1908 498 1805 2579 Fragments arranged by size: 2579 1908 1805 498 Bpu10I CC'TnA_GC Cuts at: 2880 3225 2880 Size: 345 6445 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4915 5780 6078 4915 Size: 865 298 5627 Fragments arranged by size: 5627 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5453 882 Size: 617 3954 2219 Fragments arranged by size: 3954 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4581 493 Size: 711 3377 2702 Fragments arranged by size: 3377 2702 711 BsaBI GATnn'nnATC Cuts at: 6784 6784 Size: 6790 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2664 4597 4979 5614 Size: 53 83 186 936 1131 1933 382 635 Cuts at: 5614 275 Size: 1451 Fragments arranged by size: 1933 1451 1131 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2434 3096 5223 5621 5895 6042 6453 2434 Size: 662 2127 398 274 147 411 2771 Fragments arranged by size: 2771 2127 662 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2328 2358 2593 2623 3170 Size: 30 165 30 925 30 235 30 547 Cuts at: 3170 3200 3382 3412 1178 Size: 30 182 30 4556 Fragments arranged by size: 4556 925 547 235 182 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6790 BseYI C'CCAG_C Cuts at: 2410 3604 5993 6489 2410 Size: 1194 2389 496 2711 Fragments arranged by size: 2711 2389 1194 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6217 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4240 5001 6029 1540 Size: 275 186 2239 761 1028 2301 Fragments arranged by size: 2301 2239 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2886 4851 4936 6007 2886 Size: 1965 85 1071 3669 Fragments arranged by size: 3669 1965 1071 85 BsmI GAATG_Cn' Cuts at: 4301 4394 4301 Size: 93 6697 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2864 2886 3248 3631 4109 4851 4936 Size: 399 1074 22 362 383 478 742 85 Cuts at: 4936 6007 1391 Size: 1071 2174 Fragments arranged by size: 2174 1074 1071 742 478 399 383 362 85 22 BspEI T'CCGG_A Cuts at: 6453 6453 Size: 6790 BspHI T'CATG_A Cuts at: 2146 4679 6409 2146 Size: 2533 1730 2527 Fragments arranged by size: 2533 2527 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3904 4145 844 Size: 3060 241 3489 Fragments arranged by size: 3489 3060 241 BsrBI CCG'CTC Cuts at: 1394 1823 2895 4677 1394 Size: 429 1072 1782 3507 Fragments arranged by size: 3507 1782 1072 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5285 5459 65 Size: 1183 4037 174 1396 Fragments arranged by size: 4037 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3096 3229 5434 5621 1700 Size: 110 1286 133 2205 187 2869 Fragments arranged by size: 2869 2205 1286 187 133 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6790 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6790 BssSI C'ACGA_G Cuts at: 4850 5862 4850 Size: 1012 5778 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6790 BstBI TT'CG_AA Cuts at: 5594 5594 Size: 6790 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6790 BstYI r'GATC_y Cuts at: 1972 2554 3304 3934 4120 4487 4872 4889 6341 Size: 582 750 630 186 367 385 17 1452 Cuts at: 6341 6427 6439 6785 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 750 630 582 385 367 346 186 86 17 12 BstZ17I GTA'TAC Cuts at: 2535 3209 2535 Size: 674 6116 BtgI C'CryG_G Cuts at: 513 1065 2997 513 Size: 552 1932 4306 Fragments arranged by size: 4306 1932 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6051 Fragments arranged by size: 6051 683 56 BtsI GCAGTG_nn' Cuts at: 2355 4306 5100 5120 2355 Size: 1951 794 20 4025 Fragments arranged by size: 4025 1951 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6790 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6755 DraI TTT'AAA Cuts at: 2745 4094 2745 Size: 1349 5441 DraIII CAC_nnn'GTG Cuts at: 3638 3638 Size: 6790 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5797 809 Size: 507 4481 1802 Fragments arranged by size: 4481 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4237 5126 6755 Size: 54 1425 50 275 18 2407 889 1629 Cuts at: 6755 8 Size: 43 Fragments arranged by size: 2407 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4237 1537 Size: 275 2425 4090 Fragments arranged by size: 4090 2425 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3411 4720 1353 Size: 2058 1309 3423 Fragments arranged by size: 3423 2058 1309 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5377 5751 5897 387 Size: 1064 138 3788 374 146 1280 Fragments arranged by size: 3788 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2884 2884 Size: 6790 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2526 2535 3054 3147 3606 3645 4853 Size: 393 894 9 519 93 459 39 1208 Cuts at: 4853 5450 6237 6505 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1208 894 787 597 519 459 393 268 93 39 9 EcoO109I rG'GnC_Cy Cuts at: 2501 2501 Size: 6790 EcoRI G'AATT_C Cuts at: 4099 4477 4099 Size: 378 6412 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3512 3512 Size: 6790 FspI TGC'GCA Cuts at: 5296 5296 Size: 6790 HaeII r_GCGC'y Cuts at: 1678 5937 1678 Size: 4259 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2102 2134 2141 2173 2429 Size: 32 135 32 530 32 7 32 256 Cuts at: 2429 2461 2654 2686 2831 2863 2879 2911 3104 Size: 32 193 32 145 32 16 32 193 Cuts at: 3104 3136 3170 3202 3494 3526 3671 3703 3911 Size: 32 34 32 292 32 145 32 208 Cuts at: 3911 3943 5431 5463 5505 5537 1373 Size: 32 1488 32 42 32 2626 Fragments arranged by size: 2626 1488 530 292 256 208 193 193 145 145 135 42 34 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 16 7 HincII GTy'rAC Cuts at: 669 1657 1938 2127 4134 4380 669 Size: 988 281 189 2007 246 3079 Fragments arranged by size: 3079 2007 988 281 246 189 HindIII A'AGCT_T Cuts at: 748 3371 3782 748 Size: 2623 411 3756 Fragments arranged by size: 3756 2623 411 HpaI GTT'AAC Cuts at: 4380 4380 Size: 6790 KpnI G_GTAC'C Cuts at: 4115 4115 Size: 6790 MfeI C'AATT_G Cuts at: 4389 4389 Size: 6790 MluI A'CGCG_T Cuts at: 6666 6666 Size: 6790 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3156 5904 6088 1305 Size: 1851 2748 184 2007 Fragments arranged by size: 2748 2007 1851 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4968 Fragments arranged by size: 4968 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4748 5107 5266 518 Size: 648 3582 359 159 2042 Fragments arranged by size: 3582 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2267 2414 2702 3158 3945 4206 4883 6031 Size: 661 147 288 456 787 261 677 1148 Cuts at: 6031 6276 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 787 677 661 456 288 261 245 147 NaeI GCC'GGC Cuts at: 3231 3231 Size: 6790 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6238 NdeI CA'TA_TG Cuts at: 387 387 Size: 6790 NgoMIV G'CCGG_C Cuts at: 3229 3229 Size: 6790 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6790 NotI GC'GGCC_GC Cuts at: 4237 4237 Size: 6790 NspI r_CATG'y Cuts at: 1132 1229 4148 5693 1132 Size: 97 2919 1545 2229 Fragments arranged by size: 2919 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6790 PciI A'CATG_T Cuts at: 5689 5689 Size: 6790 PflMI CCAn_nnn'nTGG Cuts at: 1108 2047 3314 1108 Size: 939 1267 4584 Fragments arranged by size: 4584 1267 939 PfoI T'CCnGG_A Cuts at: 3828 3828 Size: 6790 PmeI GTTT'AAAC Cuts at: 4094 4094 Size: 6790 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2868 2900 4859 4891 6372 6404 2868 Size: 32 1959 32 1481 32 3254 Fragments arranged by size: 3254 1959 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2501 2501 Size: 6790 PshAI GACnn'nnGTC Cuts at: 1613 3945 1613 Size: 2332 4458 PsiI TTA'TAA Cuts at: 4360 4360 Size: 6790 PspXI vC'TCGA_Gb Cuts at: 2837 2837 Size: 6790 PstI C_TGCA'G Cuts at: 830 3901 4142 830 Size: 3071 241 3478 Fragments arranged by size: 3478 3071 241 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6790 PvuII CAG'CTG Cuts at: 2267 2414 2702 3945 2267 Size: 147 288 1243 5112 Fragments arranged by size: 5112 1243 288 147 SacI G_AGCT'C Cuts at: 2886 2886 Size: 6790 SalI G'TCGA_C Cuts at: 1936 4132 1936 Size: 2196 4594 SbfI CC_TGCA'GG Cuts at: 4142 4142 Size: 6790 ScaI AGT'ACT Cuts at: 5038 6750 5038 Size: 1712 5078 SfcI C'TryA_G Cuts at: 826 1045 2658 2697 2733 3264 3897 3957 4138 Size: 219 1613 39 36 531 633 60 181 Cuts at: 4138 5273 5954 6145 826 Size: 1135 681 191 1471 Fragments arranged by size: 1613 1471 1135 681 633 531 219 191 181 60 39 36 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6790 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6790 SmaI CCC'GGG Cuts at: 1872 4117 1872 Size: 2245 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2837 4894 5795 6057 820 Size: 197 926 894 2057 901 262 1553 Fragments arranged by size: 2057 1553 926 901 894 262 197 SnaBI TAC'GTA Cuts at: 493 4581 493 Size: 4088 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6790 SphI G_CATG'C Cuts at: 1132 4148 1132 Size: 3016 3774 SspI AAT'ATT Cuts at: 5 52 4714 5 Size: 47 4662 2081 Fragments arranged by size: 4662 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6790 StyI C'CwwG_G Cuts at: 513 1065 2022 3273 3675 4229 513 Size: 552 957 1251 402 554 3074 Fragments arranged by size: 3074 1251 957 554 552 402 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3493 4817 4834 4987 5172 5617 325 Size: 1255 1913 1324 17 153 185 445 1498 Fragments arranged by size: 1913 1498 1324 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2979 5036 6748 Size: 274 80 33 51 1443 1002 2057 1712 Cuts at: 6748 96 Size: 138 Fragments arranged by size: 2057 1712 1443 1002 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2593 2625 3309 3341 6750 6782 2593 Size: 32 684 32 3409 32 2601 Fragments arranged by size: 3409 2601 684 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6790 XbaI T'CTAG_A Cuts at: 4126 4126 Size: 6790 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2137 3586 2137 Size: 1449 5341 XhoI C'TCGA_G Cuts at: 1943 2837 1943 Size: 894 5896 XmaI C'CCGG_G Cuts at: 1870 4115 1870 Size: 2245 4545 XmnI GAAnn'nnTTC Cuts at: 3393 3471 4919 3393 Size: 78 1448 5264 Fragments arranged by size: 5264 1448 78 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4598 276 Size: 53 83 186 936 3064 2468 Fragments arranged by size: 3064 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI Bsu36I EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI