Restriction map for
pFN21AB9389
(Linear) (Six-base) MAPSORT of: KIBB9389.seq Check: 9512 from: 1 to: 2085
KIBB9389.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 0 529 1203 2085
Size: 529 674 882
Fragments arranged by size:
882 674 529
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 521 530 1049 1601 2085
Size: 521 9 519 552 484
Fragments arranged by size:
552 521 519 484 9
AgeI A'CCGG_T
Cuts at: 0 1091 2085
Size: 1091 994
AhdI GACnn_n'nnGTC
Cuts at: 0 663 2085
Size: 663 1422
AlwNI CAG_nnn'CTG
Cuts at: 0 103 700 874 1027 1165 2085
Size: 103 597 174 153 138 920
Fragments arranged by size:
920 597 174 153 138 103
ApoI r'AATT_y
Cuts at: 0 1546 2085
Size: 1546 539
AvaI C'yCGr_G
Cuts at: 0 832 2085
Size: 832 1253
BanI G'GyrC_C
Cuts at: 0 856 2085
Size: 856 1229
BanII G_rGCy'C
Cuts at: 0 881 1626 2085
Size: 881 745 459
Fragments arranged by size:
881 745 459
BbsI GAAGACnn'nnnn_
Cuts at: 0 1361 1490 2085
Size: 1361 129 595
Fragments arranged by size:
1361 595 129
BciVI GTATCCnnnnn_n'
Cuts at: 0 804 2085
Size: 804 1281
BclI T'GATC_A
Cuts at: 0 822 2085
Size: 822 1263
BglII A'GATC_T
Cuts at: 0 1929 2085
Size: 1929 156
Bme1580I G_kGCm'C
Cuts at: 0 859 1243 2085
Size: 859 384 842
Fragments arranged by size:
859 842 384
BmrI ACTGGGnnnn_n'
Cuts at: 0 862 2085
Size: 862 1223
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1533 2085
Size: 1533 552
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1142 1640 2085
Size: 1142 498 445
Fragments arranged by size:
1142 498 445
Bpu10I CC'TnA_GC
Cuts at: 0 875 1220 2085
Size: 875 345 865
Fragments arranged by size:
875 865 345
BsaHI Gr'CG_yC
Cuts at: 0 659 2085
Size: 659 1426
BsaWI w'CCGG_w
Cuts at: 0 429 1091 2085
Size: 429 662 994
Fragments arranged by size:
994 662 429
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 323 353 588 618 1165 1195 1377 1407
Size: 323 30 235 30 547 30 182 30
Cuts at: 1407 2085
Size: 678
Fragments arranged by size:
678 547 323 235 182 30 30 30
30
BseYI C'CCAG_C
Cuts at: 0 405 1599 2085
Size: 405 1194 486
Fragments arranged by size:
1194 486 405
BsiHKAI G_wGCw'C
Cuts at: 0 881 2085
Size: 881 1204
Bsp1286I G_dGCh'C
Cuts at: 0 859 881 1243 1626 2085
Size: 859 22 362 383 459
Fragments arranged by size:
859 459 383 362 22
BspHI T'CATG_A
Cuts at: 0 141 2085
Size: 141 1944
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1899 2085
Size: 1899 186
BsrBI CCG'CTC
Cuts at: 0 890 2085
Size: 890 1195
BsrFI r'CCGG_y
Cuts at: 0 1091 1224 2085
Size: 1091 133 861
Fragments arranged by size:
1091 861 133
BstYI r'GATC_y
Cuts at: 0 549 1299 1929 2085
Size: 549 750 630 156
Fragments arranged by size:
750 630 549 156
BstZ17I GTA'TAC
Cuts at: 0 530 1204 2085
Size: 530 674 881
Fragments arranged by size:
881 674 530
BtgI C'CryG_G
Cuts at: 0 992 2085
Size: 992 1093
BtsI GCAGTG_nn'
Cuts at: 0 350 2085
Size: 350 1735
DraI TTT'AAA
Cuts at: 0 740 2085
Size: 740 1345
DraIII CAC_nnn'GTG
Cuts at: 0 1633 2085
Size: 1633 452
EarI CTCTTCn'nnn_
Cuts at: 0 1406 2085
Size: 1406 679
EcoICRI GAG'CTC
Cuts at: 0 879 2085
Size: 879 1206
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 521 530 1049 1142 1601 1640 2085
Size: 521 9 519 93 459 39 445
Fragments arranged by size:
521 519 459 445 93 39 9
EcoO109I rG'GnC_Cy
Cuts at: 0 496 2085
Size: 496 1589
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1507 2085
Size: 1507 578
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 97 129 136 168 424 456 649 681
Size: 97 32 7 32 256 32 193 32
Cuts at: 681 826 858 874 906 1099 1131 1165 1197
Size: 145 32 16 32 193 32 34 32
Cuts at: 1197 1489 1521 1666 1698 1906 1938 2085
Size: 292 32 145 32 208 32 147
Fragments arranged by size:
292 256 208 193 193 147 145 145
97 34 32 32 32 32 32 32
32 32 32 32 32 16 7
HincII GTy'rAC
Cuts at: 0 122 2085
Size: 122 1963
HindIII A'AGCT_T
Cuts at: 0 1366 1777 2085
Size: 1366 411 308
Fragments arranged by size:
1366 411 308
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1151 2085
Size: 1151 934
MspA1I CmG'CkG
Cuts at: 0 262 409 697 1153 1940 2085
Size: 262 147 288 456 787 145
Fragments arranged by size:
787 456 288 262 147 145
NaeI GCC'GGC
Cuts at: 0 1226 2085
Size: 1226 859
NgoMIV G'CCGG_C
Cuts at: 0 1224 2085
Size: 1224 861
PflMI CCAn_nnn'nTGG
Cuts at: 0 42 1309 2085
Size: 42 1267 776
Fragments arranged by size:
1267 776 42
PfoI T'CCnGG_A
Cuts at: 0 1823 2085
Size: 1823 262
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 863 895 2085
Size: 863 32 1190
Fragments arranged by size:
1190 863 32
PpuMI rG'GwC_Cy
Cuts at: 0 496 2085
Size: 496 1589
PshAI GACnn'nnGTC
Cuts at: 0 1940 2085
Size: 1940 145
PspXI vC'TCGA_Gb
Cuts at: 0 832 2085
Size: 832 1253
PstI C_TGCA'G
Cuts at: 0 1896 2085
Size: 1896 189
PvuII CAG'CTG
Cuts at: 0 262 409 697 1940 2085
Size: 262 147 288 1243 145
Fragments arranged by size:
1243 288 262 147 145
SacI G_AGCT'C
Cuts at: 0 881 2085
Size: 881 1204
SfcI C'TryA_G
Cuts at: 0 653 692 728 1259 1892 1952 2085
Size: 653 39 36 531 633 60 133
Fragments arranged by size:
653 633 531 133 60 39 36
SmlI C'TyrA_G
Cuts at: 0 832 2085
Size: 832 1253
StyI C'CwwG_G
Cuts at: 0 17 1268 1670 2085
Size: 17 1251 402 415
Fragments arranged by size:
1251 415 402 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1488 2085
Size: 1488 597
TatI w'GTAC_w
Cuts at: 0 974 2085
Size: 974 1111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 588 620 1304 1336 2085
Size: 588 32 684 32 749
Fragments arranged by size:
749 684 588 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 132 1581 2085
Size: 132 1449 504
Fragments arranged by size:
1449 504 132
XhoI C'TCGA_G
Cuts at: 0 832 2085
Size: 832 1253
XmnI GAAnn'nnTTC
Cuts at: 0 1388 1466 2085
Size: 1388 78 619
Fragments arranged by size:
1388 619 78
Enzymes that do cut:
AccI AcuI AgeI AhdI AlwNI ApoI AvaI BanI
BanII BbsI BciVI BclI BglII Bme1580I BmrI BplI
BpmI Bpu10I BsaHI BsaWI BsaXI BseYI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrFI BstYI BstZ17I BtgI BtsI
DraI DraIII EarI EcoICRI Eco57MI EcoO109I FalI Hin4I
HincII HindIII MmeI MspA1I NaeI NgoMIV PflMI PfoI
PpiI PpuMI PshAI PspXI PstI PvuII SacI SfcI
SmlI StyI TaqII TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BfrBI BglI BlpI
BmgBI BmtI BpuEI BsaI BsaAI BsaBI BseRI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI Bsu36I BtgZI ClaI
CspCI DrdI EaeI EagI EciI EcoNI EcoRI EcoRV
FseI FspI FspAI HaeII HpaI KasI KpnI MfeI
MluI MscI MslI NarI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PsiI PspOMI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XmaI ZraI