Restriction map for
pFN21AB5588
(Circular) (Six-base) MAPSORT of: pFN21AB5588.seq Check: 4080 from: 1 to: 6880
pFN21AB5588 6880 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2611 3498 2611
Size: 887 5993
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4690 278
Size: 53 83 186 936 3154 2468
Fragments arranged by size:
3154 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2532 2563 4082 4223 1937
Size: 595 31 1519 141 4594
Fragments arranged by size:
4594 1519 595 141 31
Acc65I G'GTAC_C
Cuts at: 4201 4201
Size: 6880
AclI AA'CG_TT
Cuts at: 1563 5007 5380 1563
Size: 3444 373 3063
Fragments arranged by size:
3444 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2675 2761 3785 3840 4173 4943 6327 6595
Size: 1043 86 1024 55 333 770 1384 268
Cuts at: 6595 1632
Size: 1917
Fragments arranged by size:
1917 1384 1043 1024 770 333 268 86
55
AfeI AGC'GCT
Cuts at: 2516 2516
Size: 6880
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6683
AflIII A'CryG_T
Cuts at: 1203 5779 6756 1203
Size: 4576 977 1327
Fragments arranged by size:
4576 1327 977
AgeI A'CCGG_T
Cuts at: 5711 5711
Size: 6880
AhdI GACnn_n'nnGTC
Cuts at: 3649 3835 5609 3649
Size: 186 1774 4920
Fragments arranged by size:
4920 1774 186
AleI CACnn'nnGTG
Cuts at: 1166 3609 1166
Size: 2443 4437
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6880
AlwNI CAG_nnn'CTG
Cuts at: 4623 6195 4623
Size: 1572 5308
ApaI G_GGCC'C
Cuts at: 4168 4168
Size: 6880
ApaLI G'TGCA_C
Cuts at: 4937 6093 4937
Size: 1156 5724
ApoI r'AATT_y
Cuts at: 1493 4189 4415 4567 6676 1493
Size: 2696 226 152 2109 1697
Fragments arranged by size:
2696 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5434 160
Size: 872 4402 1606
Fragments arranged by size:
4402 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6880
AvaI C'yCGr_G
Cuts at: 1870 1943 2061 2994 3084 3372 4205 1870
Size: 73 118 933 90 288 833 4545
Fragments arranged by size:
4545 933 833 288 118 90 73
AvrII C'CTAG_G
Cuts at: 2902 2902
Size: 6880
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3322 3355 3322
Size: 33 6847
BamHI G'GATC_C
Cuts at: 4210 4577 4210
Size: 367 6513
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3894 4201 5656 618
Size: 325 217 627 2107 307 1455 1842
Fragments arranged by size:
2107 1842 1455 627 325 307 217
BanII G_rGCy'C
Cuts at: 1391 2529 3083 3372 3993 4168 4199 1391
Size: 1138 554 289 621 175 31 4072
Fragments arranged by size:
4072 1138 621 554 289 175 31
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3426 3652 928
Size: 971 1527 226 4156
Fragments arranged by size:
4156 1527 971 226
BbvCI CC'TCA_GC
Cuts at: 3881 3881
Size: 6880
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5071 5105 1903
Size: 34 3134 34 3678
Fragments arranged by size:
3678 3134 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3323 4772 5982 3323
Size: 1449 1210 4221
Fragments arranged by size:
4221 1449 1210
BclI T'GATC_A
Cuts at: 1548 2394 3283 1548
Size: 846 889 5145
Fragments arranged by size:
5145 889 846
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2037 4016 5491
Size: 107 122 71 631 605 365 1979 1475
Cuts at: 5491 136
Size: 1525
Fragments arranged by size:
1979 1525 1475 631 605 365 122 107
71
BglII A'GATC_T
Cuts at: 2017 6875 2017
Size: 4858 2022
BlpI GC'TnA_GC
Cuts at: 4297 4297
Size: 6880
Bme1580I G_kGCm'C
Cuts at: 1790 2882 4168 4941 6097 1790
Size: 1092 1286 773 1156 2573
Fragments arranged by size:
2573 1286 1156 1092 773
BmgBI CAC'GTC
Cuts at: 1318 2557 3166 1318
Size: 1239 609 5032
Fragments arranged by size:
5032 1239 609
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3330 4611 5569
Size: 416 53 83 399 24 1913 1281 958
Cuts at: 5569 442
Size: 1753
Fragments arranged by size:
1913 1753 1281 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6880
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2200 2708 3165 3689 3716 3959 4070 5540
Size: 961 508 457 524 27 243 111 1470
Cuts at: 5540 1239
Size: 2579
Fragments arranged by size:
2579 1470 961 524 508 457 243 111
27
Bpu10I CC'TnA_GC
Cuts at: 3366 3881 3366
Size: 515 6365
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3073 5005 5870 6168 3073
Size: 1932 865 298 3785
Fragments arranged by size:
3785 1932 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3270 5543 882
Size: 617 1771 2273 2219
Fragments arranged by size:
2273 2219 1771 617
BsaAI yAC'GTr
Cuts at: 493 1204 2506 4671 493
Size: 711 1302 2165 2702
Fragments arranged by size:
2702 2165 1302 711
BsaBI GATnn'nnATC
Cuts at: 2642 6874 2642
Size: 4232 2648
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2765 4687 5069 5704
Size: 53 83 186 936 1232 1922 382 635
Cuts at: 5704 275
Size: 1451
Fragments arranged by size:
1922 1451 1232 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2445 2949 5313 5711 5985 6132 6543 2445
Size: 504 2364 398 274 147 411 2782
Fragments arranged by size:
2782 2364 504 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3105 3135 1178
Size: 30 165 30 1702 30 4923
Fragments arranged by size:
4923 1702 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2439 2606 3458 3466 3650 4190 1186
Size: 1253 167 852 8 184 540 3876
Fragments arranged by size:
3876 1253 852 540 184 167 8
BseYI C'CCAG_C
Cuts at: 2478 6083 6579 2478
Size: 3605 496 2779
Fragments arranged by size:
3605 2779 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3902 1163
Size: 573 2166 4141
Fragments arranged by size:
4141 2166 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2422 3507 4330 5091 6119 1540
Size: 275 186 421 1085 823 761 1028 2301
Fragments arranged by size:
2301 1085 1028 823 761 421 275 186
BsiHKAI G_wGCw'C
Cuts at: 2694 3675 4941 5026 6097 2694
Size: 981 1266 85 1071 3477
Fragments arranged by size:
3477 1266 1071 981 85
BsmI GAATG_Cn'
Cuts at: 3984 4391 4484 3984
Size: 407 93 6380
Fragments arranged by size:
6380 407 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2867 2886 3141 2867
Size: 19 255 6606
Fragments arranged by size:
6606 255 19
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2529 2694 2882 3083 3372 3675 3993
Size: 399 739 165 188 201 289 303 318
Cuts at: 3993 4168 4199 4941 5026 6097 1391
Size: 175 31 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 739 399 318 303 289
201 188 175 165 85 31
BspEI T'CCGG_A
Cuts at: 2445 6543 2445
Size: 4098 2782
BspHI T'CATG_A
Cuts at: 4769 6499 4769
Size: 1730 5150
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2611 3498 4235 844
Size: 1767 887 737 3489
Fragments arranged by size:
3489 1767 887 737
BsrBI CCG'CTC
Cuts at: 1394 1823 2206 4767 1394
Size: 429 383 2561 3507
Fragments arranged by size:
3507 2561 429 383
BsrDI GCAATG_nn'
Cuts at: 65 1248 2436 5375 5549 65
Size: 1183 1188 2939 174 1396
Fragments arranged by size:
2939 1396 1188 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3108 5524 5711 1700
Size: 110 1298 2416 187 2869
Fragments arranged by size:
2869 2416 1298 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6880
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6880
BssSI C'ACGA_G
Cuts at: 3605 4940 5952 3605
Size: 1335 1012 4533
Fragments arranged by size:
4533 1335 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6880
BstBI TT'CG_AA
Cuts at: 5684 5684
Size: 6880
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6880
BstXI CCAn_nnnn'nTGG
Cuts at: 2506 2623 2837 3611 2506
Size: 117 214 774 5775
Fragments arranged by size:
5775 774 214 117
BstYI r'GATC_y
Cuts at: 1972 2017 2842 2862 4210 4577 4962 4979 6431
Size: 45 825 20 1348 367 385 17 1452
Cuts at: 6431 6517 6529 6875 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1348 825 385 367 346 86
45 20 17 12
BstZ17I GTA'TAC
Cuts at: 4083 4083
Size: 6880
BtgI C'CryG_G
Cuts at: 513 1065 2004 2911 3507 3591 513
Size: 552 939 907 596 84 3802
Fragments arranged by size:
3802 939 907 596 552 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2760 4118 487
Size: 683 56 1534 1358 3249
Fragments arranged by size:
3249 1534 1358 683 56
BtsI GCAGTG_nn'
Cuts at: 2489 3015 3930 4396 5190 5210 2489
Size: 526 915 466 794 20 4159
Fragments arranged by size:
4159 915 794 526 466 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6880
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3218 3253 529
Size: 35 2654 35 4156
Fragments arranged by size:
4156 2654 35 35
DraI TTT'AAA
Cuts at: 4184 4184
Size: 6880
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5887 809
Size: 507 4571 1802
Fragments arranged by size:
4571 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2050 2068 2419
Size: 54 1425 50 275 18 220 18 351
Cuts at: 2419 2803 3110 3201 3239 3382 3504 3588 3871
Size: 384 307 91 38 143 122 84 283
Cuts at: 3871 4008 4128 4327 5216 6845 8
Size: 137 120 199 889 1629 43
Fragments arranged by size:
1629 1425 889 384 351 307 283 275
220 199 143 137 122 120 91 84
54 50 43 38 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2419 3504 4327 1537
Size: 275 607 1085 823 4090
Fragments arranged by size:
4090 1085 823 607 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2684 2930 3771 4149 4810 1353
Size: 1331 246 841 378 661 3423
Fragments arranged by size:
3423 1331 841 661 378 246
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2018 2747 5467 5841 5987 387
Size: 1064 138 429 729 2720 374 146 1280
Fragments arranged by size:
2720 1280 1064 729 429 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2200 2675 2708 2761 3165 3689 3716
Size: 393 568 475 33 53 404 524 27
Cuts at: 3716 3785 3840 3959 4070 4173 4943 5540 6327
Size: 69 55 119 111 103 770 597 787
Cuts at: 6327 6595 1239
Size: 268 1524
Fragments arranged by size:
1524 787 770 597 568 524 475 404
393 268 119 111 103 69 55 53
33 27
EcoO109I rG'GnC_Cy
Cuts at: 2223 3093 3913 4057 2223
Size: 870 820 144 5046
Fragments arranged by size:
5046 870 820 144
EcoRI G'AATT_C
Cuts at: 4189 4567 4189
Size: 378 6502
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2695 3067 2695
Size: 372 6508
FspI TGC'GCA
Cuts at: 5386 5386
Size: 6880
HaeII r_GCGC'y
Cuts at: 1678 2518 2924 3619 6027 1678
Size: 840 406 695 2408 2531
Fragments arranged by size:
2531 2408 840 695 406
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2129 2161 2392 2424 3635
Size: 32 135 32 557 32 231 32 1211
Cuts at: 3635 3667 3821 3853 5521 5553 5595 5627 1373
Size: 32 154 32 1668 32 42 32 2626
Fragments arranged by size:
2626 1668 1211 557 231 154 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4224 4470 669
Size: 988 281 2286 246 3079
Fragments arranged by size:
3079 2286 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6880
HpaI GTT'AAC
Cuts at: 4470 4470
Size: 6880
KpnI G_GTAC'C
Cuts at: 4205 4205
Size: 6880
MfeI C'AATT_G
Cuts at: 4479 4479
Size: 6880
MluI A'CGCG_T
Cuts at: 6756 6756
Size: 6880
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2487 2523 2751 5994 6178 1305
Size: 1182 36 228 3243 184 2007
Fragments arranged by size:
3243 2007 1182 228 184 36
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2070 3241 3384 3590 3873
Size: 54 1425 343 238 1171 143 206 283
Cuts at: 3873 4010 4130 10
Size: 137 120 2760
Fragments arranged by size:
2760 1425 1171 343 283 238 206 143
137 120 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2076 2636 3609 4838 5197 5356 518
Size: 648 910 560 973 1229 359 159 2042
Fragments arranged by size:
2042 1229 973 910 648 560 359 159
MspA1I CmG'CkG
Cuts at: 1606 2220 2812 2913 3327 3818 4296 4973 6121
Size: 614 592 101 414 491 478 677 1148
Cuts at: 6121 6366 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 614 592 491 478 414
245 101
NaeI GCC'GGC
Cuts at: 3110 3110
Size: 6880
NcoI C'CATG_G
Cuts at: 513 1065 2004 3591 513
Size: 552 939 1587 3802
Fragments arranged by size:
3802 1587 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6880
NgoMIV G'CCGG_C
Cuts at: 3108 3108
Size: 6880
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6880
NotI GC'GGCC_GC
Cuts at: 4327 4327
Size: 6880
NspI r_CATG'y
Cuts at: 1132 1229 2290 2477 4238 5783 1132
Size: 97 1061 187 1761 1545 2229
Fragments arranged by size:
2229 1761 1545 1061 187 97
PasI CC'CwG_GG
Cuts at: 1348 2298 1348
Size: 950 5930
PciI A'CATG_T
Cuts at: 5779 5779
Size: 6880
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2583 1108
Size: 1475 5405
PmeI GTTT'AAAC
Cuts at: 4184 4184
Size: 6880
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4013 4045 4949 4981 6462 6494 4013
Size: 32 904 32 1481 32 4399
Fragments arranged by size:
4399 1481 904 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2223 3093 4057 2223
Size: 870 964 5046
Fragments arranged by size:
5046 964 870
PshAI GACnn'nnGTC
Cuts at: 1613 2559 1613
Size: 946 5934
PsiI TTA'TAA
Cuts at: 4450 4450
Size: 6880
PspOMI G'GGCC_C
Cuts at: 4164 4164
Size: 6880
PstI C_TGCA'G
Cuts at: 830 4232 830
Size: 3402 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6880
PvuII CAG'CTG
Cuts at: 2812 2812
Size: 6880
SacII CC_GC'GG
Cuts at: 2914 2914
Size: 6880
SalI G'TCGA_C
Cuts at: 1936 4222 1936
Size: 2286 4594
SanDI GG'GwC_CC
Cuts at: 2223 2223
Size: 6880
SapI GCTCTTCn'nnn_
Cuts at: 2930 2930
Size: 6880
SbfI CC_TGCA'GG
Cuts at: 4232 4232
Size: 6880
ScaI AGT'ACT
Cuts at: 2180 4139 5128 6840 2180
Size: 1959 989 1712 2220
Fragments arranged by size:
2220 1959 1712 989
SfcI C'TryA_G
Cuts at: 826 1045 2182 2564 4228 5363 6044 6235 826
Size: 219 1137 382 1664 1135 681 191 1471
Fragments arranged by size:
1664 1471 1137 1135 681 382 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 4016 4016
Size: 6880
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6880
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6880
SmaI CCC'GGG
Cuts at: 1872 2996 4207 1872
Size: 1124 1211 4545
Fragments arranged by size:
4545 1211 1124
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3052 4984 5885 6147 820
Size: 197 926 1109 1932 901 262 1553
Fragments arranged by size:
1932 1553 1109 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4671 493
Size: 4178 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6880
SphI G_CATG'C
Cuts at: 1132 2477 4238 1132
Size: 1345 1761 3774
Fragments arranged by size:
3774 1761 1345
SspI AAT'ATT
Cuts at: 5 52 4804 5
Size: 47 4752 2081
Fragments arranged by size:
4752 2081 47
StuI AGG'CCT
Cuts at: 1517 2774 4160 1517
Size: 1257 1386 4237
Fragments arranged by size:
4237 1386 1257
StyI C'CwwG_G
Cuts at: 513 1065 2004 2166 2902 3591 4319 513
Size: 552 939 162 736 689 728 3074
Fragments arranged by size:
3074 939 736 728 689 552 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4907 4924 5077 5262 5707 325
Size: 1255 3327 17 153 185 445 1498
Fragments arranged by size:
3327 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2178 2544 2709
Size: 274 80 33 51 1443 201 366 165
Cuts at: 2709 2718 3723 4137 5126 6838 96
Size: 9 1005 414 989 1712 138
Fragments arranged by size:
1712 1443 1005 989 414 366 274 201
165 138 80 51 33 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3105 3137 6840 6872 3105
Size: 32 3703 32 3113
Fragments arranged by size:
3703 3113 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6880
XbaI T'CTAG_A
Cuts at: 3289 4216 3289
Size: 927 5953
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3709 3847 3709
Size: 138 6742
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6880
XmaI C'CCGG_G
Cuts at: 1870 2994 4205 1870
Size: 1124 1211 4545
Fragments arranged by size:
4545 1211 1124
XmnI GAAnn'nnTTC
Cuts at: 5009 5009
Size: 6880
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4688 276
Size: 53 83 186 936 3154 2468
Fragments arranged by size:
3154 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacII SalI SanDI SapI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BbeI BfrBI BplI BsiWI Bsu36I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI
SexAI SfoI SrfI SwaI