(Circular) (Six-base) MAPSORT of: pFN21AB3144.seq Check: 1418 from: 1 to: 6430 pFN21AB3144 6430 bp With 182 enzymes: SgfI * October 20, 2009 10:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2452 2583 2587 2452 Size: 131 4 6295 Fragments arranged by size: 6295 131 4 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4240 278 Size: 53 83 186 936 2704 2468 Fragments arranged by size: 2704 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3773 1937 Size: 1836 4594 Acc65I G'GTAC_C Cuts at: 3751 3751 Size: 6430 AclI AA'CG_TT Cuts at: 1563 3431 4557 4930 1563 Size: 1868 1126 373 3063 Fragments arranged by size: 3063 1868 1126 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3191 3648 3694 4493 5877 6145 1632 Size: 1559 457 46 799 1384 268 1917 Fragments arranged by size: 1917 1559 1384 799 457 268 46 AfeI AGC'GCT Cuts at: 3218 3218 Size: 6430 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6233 AflIII A'CryG_T Cuts at: 1203 5329 6306 1203 Size: 4126 977 1327 Fragments arranged by size: 4126 1327 977 AgeI A'CCGG_T Cuts at: 5261 5261 Size: 6430 AhdI GACnn_n'nnGTC Cuts at: 5159 5159 Size: 6430 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6430 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3498 1184 Size: 2314 4116 AlwNI CAG_nnn'CTG Cuts at: 2611 2686 4173 5745 2611 Size: 75 1487 1572 3296 Fragments arranged by size: 3296 1572 1487 75 ApaI G_GGCC'C Cuts at: 2060 2374 2631 2670 2060 Size: 314 257 39 5820 Fragments arranged by size: 5820 314 257 39 ApaLI G'TGCA_C Cuts at: 4487 5643 4487 Size: 1156 5274 ApoI r'AATT_y Cuts at: 1493 3282 3739 3965 4117 6226 1493 Size: 1789 457 226 152 2109 1697 Fragments arranged by size: 2109 1789 1697 457 226 152 AscI GG'CGCG_CC Cuts at: 2585 2585 Size: 6430 AseI AT'TA_AT Cuts at: 160 1032 4984 160 Size: 872 3952 1606 Fragments arranged by size: 3952 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6430 AvaI C'yCGr_G Cuts at: 1870 1943 2028 2061 2624 3755 1870 Size: 73 85 33 563 1131 4545 Fragments arranged by size: 4545 1131 563 85 73 33 BamHI G'GATC_C Cuts at: 3760 4127 3760 Size: 367 6063 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2518 2861 3161 3620 3751 Size: 325 217 627 731 343 300 459 131 Cuts at: 3751 5206 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 731 627 459 343 325 300 217 131 BanII G_rGCy'C Cuts at: 1391 2060 2205 2306 2374 2631 2670 3749 1391 Size: 669 145 101 68 257 39 1079 4072 Fragments arranged by size: 4072 1079 669 257 145 101 68 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2283 928 Size: 971 384 5075 Fragments arranged by size: 5075 971 384 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2329 2363 4621 4655 1903 Size: 34 392 34 2258 34 3678 Fragments arranged by size: 3678 2258 392 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4322 5532 4322 Size: 1210 5220 BclI T'GATC_A Cuts at: 1548 1548 Size: 6430 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2105 2420 2699 Size: 107 122 71 631 605 433 315 279 Cuts at: 2699 2724 5041 136 Size: 25 2317 1525 Fragments arranged by size: 2317 1525 631 605 433 315 279 122 107 71 25 BglII A'GATC_T Cuts at: 3000 6425 3000 Size: 3425 3005 BlpI GC'TnA_GC Cuts at: 2109 2117 2843 2976 3847 2109 Size: 8 726 133 871 4692 Fragments arranged by size: 4692 871 726 133 8 Bme1580I G_kGCm'C Cuts at: 1790 2060 2374 2523 2631 2670 2680 3116 3164 Size: 270 314 149 108 39 10 436 48 Cuts at: 3164 3625 4491 5647 1790 Size: 461 866 1156 2573 Fragments arranged by size: 2573 1156 866 461 436 314 270 149 108 48 39 10 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6430 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2427 3119 4161 Size: 416 53 83 399 24 1010 692 1042 Cuts at: 4161 5119 442 Size: 958 1753 Fragments arranged by size: 1753 1042 1010 958 692 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6430 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2290 2573 2837 3251 3272 3321 3587 5090 Size: 1051 283 264 414 21 49 266 1503 Cuts at: 5090 1239 Size: 2579 Fragments arranged by size: 2579 1503 1051 414 283 266 264 49 21 Bpu10I CC'TnA_GC Cuts at: 2514 2514 Size: 6430 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2215 2519 3365 4555 5420 5718 2215 Size: 304 846 1190 865 298 2927 Fragments arranged by size: 2927 1190 865 846 304 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3002 3540 5093 882 Size: 617 1503 538 1553 2219 Fragments arranged by size: 2219 1553 1503 617 538 BsaAI yAC'GTr Cuts at: 493 1204 4221 493 Size: 711 3017 2702 Fragments arranged by size: 3017 2702 711 BsaBI GATnn'nnATC Cuts at: 6424 6424 Size: 6430 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2472 4237 4619 5254 Size: 53 83 186 936 939 1765 382 635 Cuts at: 5254 275 Size: 1451 Fragments arranged by size: 1765 1451 939 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2137 4863 5261 5535 5682 6093 2137 Size: 2726 398 274 147 411 2474 Fragments arranged by size: 2726 2474 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2862 2892 1178 Size: 30 165 30 1459 30 4716 Fragments arranged by size: 4716 1459 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 3419 3422 3599 3602 3605 3737 1186 Size: 913 1320 3 177 3 3 132 3879 Fragments arranged by size: 3879 1320 913 177 132 3 3 3 BseYI C'CCAG_C Cuts at: 2269 2480 2679 2882 2971 3521 5633 6129 2269 Size: 211 199 203 89 550 2112 496 2570 Fragments arranged by size: 2570 2112 550 496 211 203 199 89 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2424 2561 3468 3497 1163 Size: 573 688 137 907 29 4096 Fragments arranged by size: 4096 907 688 573 137 29 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2019 2100 3880 4641 5669 1540 Size: 275 186 18 81 1780 761 1028 2301 Fragments arranged by size: 2301 1780 1028 761 275 186 81 18 BsiHKAI G_wGCw'C Cuts at: 2205 2535 4491 4576 5647 2205 Size: 330 1956 85 1071 2988 Fragments arranged by size: 2988 1956 1071 330 85 BsmI GAATG_Cn' Cuts at: 3466 3941 4034 3466 Size: 475 93 5862 Fragments arranged by size: 5862 475 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2479 2479 Size: 6430 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2060 2205 2306 2374 2523 2535 2631 Size: 399 270 145 101 68 149 12 96 Cuts at: 2631 2670 2680 3116 3164 3625 3749 4491 4576 Size: 39 10 436 48 461 124 742 85 Cuts at: 4576 5647 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 461 436 399 270 149 145 124 101 96 85 68 48 39 12 10 BspEI T'CCGG_A Cuts at: 2137 6093 2137 Size: 3956 2474 BspHI T'CATG_A Cuts at: 4319 6049 4319 Size: 1730 4700 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2452 2583 2587 3460 3519 3785 844 Size: 1608 131 4 873 59 266 3489 Fragments arranged by size: 3489 1608 873 266 131 59 4 BsrBI CCG'CTC Cuts at: 1394 1823 4317 1394 Size: 429 2494 3507 Fragments arranged by size: 3507 2494 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4925 5099 65 Size: 1183 3677 174 1396 Fragments arranged by size: 3677 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2095 2589 2714 3237 5074 5261 1700 Size: 110 285 494 125 523 1837 187 2869 Fragments arranged by size: 2869 1837 523 494 285 187 125 110 BsrGI T'GTAC_A Cuts at: 96 2919 96 Size: 2823 3607 BssHII G'CGCG_C Cuts at: 1925 2585 1925 Size: 660 5770 BssSI C'ACGA_G Cuts at: 4490 5502 4490 Size: 1012 5418 BstAPI GCAn_nnn'nTGC Cuts at: 794 2365 2967 794 Size: 1571 602 4257 Fragments arranged by size: 4257 1571 602 BstBI TT'CG_AA Cuts at: 5234 5234 Size: 6430 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6430 BstXI CCAn_nnnn'nTGG Cuts at: 2464 3229 3314 2464 Size: 765 85 5580 Fragments arranged by size: 5580 765 85 BstYI r'GATC_y Cuts at: 1972 3000 3309 3760 4127 4512 4529 5981 6067 Size: 1028 309 451 367 385 17 1452 86 Cuts at: 6067 6079 6425 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1028 451 385 367 346 309 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2697 2697 Size: 6430 BtgI C'CryG_G Cuts at: 513 1065 2418 2745 3313 513 Size: 552 1353 327 568 3630 Fragments arranged by size: 3630 1353 568 552 327 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5691 Fragments arranged by size: 5691 683 56 BtsI GCAGTG_nn' Cuts at: 2246 2684 2734 3632 3946 4740 4760 2246 Size: 438 50 898 314 794 20 3916 Fragments arranged by size: 3916 898 794 438 314 50 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6430 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6395 DraI TTT'AAA Cuts at: 3734 3734 Size: 6430 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5437 809 Size: 507 4121 1802 Fragments arranged by size: 4121 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2016 2097 2937 Size: 54 1425 50 275 18 186 81 840 Cuts at: 2937 3354 3877 4766 6395 8 Size: 417 523 889 1629 43 Fragments arranged by size: 1629 1425 889 840 523 417 275 186 81 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2016 2097 3877 1537 Size: 275 204 81 1780 4090 Fragments arranged by size: 4090 1780 275 204 81 EarI CTCTTCn'nnn_ Cuts at: 1353 3667 3714 4360 1353 Size: 2314 47 646 3423 Fragments arranged by size: 3423 2314 646 47 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5017 5391 5537 387 Size: 1064 138 3428 374 146 1280 Fragments arranged by size: 3428 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2203 2203 Size: 6430 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2290 2573 2837 3191 3251 3272 3321 Size: 393 658 283 264 354 60 21 49 Cuts at: 3321 3587 3648 3694 4493 5090 5877 6145 1239 Size: 266 61 46 799 597 787 268 1524 Fragments arranged by size: 1524 799 787 658 597 393 354 283 268 266 264 61 60 49 46 21 EcoNI CCTnn'n_nnAGG Cuts at: 2579 2898 3029 3254 2579 Size: 319 131 225 5755 Fragments arranged by size: 5755 319 225 131 EcoO109I rG'GnC_Cy Cuts at: 2056 2057 2310 2647 2666 3022 3449 3538 2056 Size: 1 253 337 19 356 427 89 4948 Fragments arranged by size: 4948 427 356 337 253 89 19 1 EcoRI G'AATT_C Cuts at: 3282 3739 4117 3282 Size: 457 378 5595 Fragments arranged by size: 5595 457 378 FseI GG_CCGG'CC Cuts at: 2099 2099 Size: 6430 FspI TGC'GCA Cuts at: 4936 4936 Size: 6430 HaeII r_GCGC'y Cuts at: 1678 2753 3220 5577 1678 Size: 1075 467 2357 2531 Fragments arranged by size: 2531 2357 1075 467 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2862 2894 5071 5103 5145 Size: 32 135 32 1290 32 2177 32 42 Cuts at: 5145 5177 1373 Size: 32 2626 Fragments arranged by size: 2626 2177 1290 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3774 4020 669 Size: 988 281 1836 246 3079 Fragments arranged by size: 3079 1836 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6430 HpaI GTT'AAC Cuts at: 4020 4020 Size: 6430 KpnI G_GTAC'C Cuts at: 3755 3755 Size: 6430 MfeI C'AATT_G Cuts at: 4029 4029 Size: 6430 MluI A'CGCG_T Cuts at: 6306 6306 Size: 6430 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2061 5544 5728 1305 Size: 756 3483 184 2007 Fragments arranged by size: 3483 2007 756 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2939 3356 10 Size: 54 1425 343 1107 417 3084 Fragments arranged by size: 3084 1425 1107 417 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2462 3578 4388 4747 4906 518 Size: 648 1296 1116 810 359 159 2042 Fragments arranged by size: 2042 1296 1116 810 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2113 2420 2975 3394 3846 4523 5671 5916 Size: 507 307 555 419 452 677 1148 245 Cuts at: 5916 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 555 507 452 419 307 245 NaeI GCC'GGC Cuts at: 2097 2591 3239 2097 Size: 494 648 5288 Fragments arranged by size: 5288 648 494 NcoI C'CATG_G Cuts at: 513 1065 2745 3313 513 Size: 552 1680 568 3630 Fragments arranged by size: 3630 1680 568 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6430 NgoMIV G'CCGG_C Cuts at: 2095 2589 3237 2095 Size: 494 648 5288 Fragments arranged by size: 5288 648 494 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6430 NotI GC'GGCC_GC Cuts at: 3877 3877 Size: 6430 NspI r_CATG'y Cuts at: 1132 1229 3788 5333 1132 Size: 97 2559 1545 2229 Fragments arranged by size: 2559 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2523 1348 Size: 1175 5255 PciI A'CATG_T Cuts at: 5329 5329 Size: 6430 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6430 PfoI T'CCnGG_A Cuts at: 2144 2306 2144 Size: 162 6268 PmeI GTTT'AAAC Cuts at: 3734 3734 Size: 6430 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4499 4531 6012 6044 4499 Size: 32 1481 32 4885 Fragments arranged by size: 4885 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2310 3449 3538 2310 Size: 1139 89 5202 Fragments arranged by size: 5202 1139 89 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6430 PsiI TTA'TAA Cuts at: 4000 4000 Size: 6430 PspOMI G'GGCC_C Cuts at: 2056 2370 2627 2666 2056 Size: 314 257 39 5820 Fragments arranged by size: 5820 314 257 39 PspXI vC'TCGA_Gb Cuts at: 2061 2061 Size: 6430 PstI C_TGCA'G Cuts at: 830 2253 2299 2580 2877 3204 3276 3303 3782 Size: 1423 46 281 297 327 72 27 479 Cuts at: 3782 830 Size: 3478 Fragments arranged by size: 3478 1423 479 327 297 281 72 46 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6430 PvuII CAG'CTG Cuts at: 2113 2975 3394 2113 Size: 862 419 5149 Fragments arranged by size: 5149 862 419 SacI G_AGCT'C Cuts at: 2205 2205 Size: 6430 SacII CC_GC'GG Cuts at: 2421 2421 Size: 6430 SalI G'TCGA_C Cuts at: 1936 3772 1936 Size: 1836 4594 SbfI CC_TGCA'GG Cuts at: 3782 3782 Size: 6430 ScaI AGT'ACT Cuts at: 4678 6390 4678 Size: 1712 4718 SfcI C'TryA_G Cuts at: 826 1045 2249 2295 2576 2873 3200 3272 3299 Size: 219 1204 46 281 297 327 72 27 Cuts at: 3299 3778 4913 5594 5785 826 Size: 479 1135 681 191 1471 Fragments arranged by size: 1471 1204 1135 681 479 327 297 281 219 191 72 46 27 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6430 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6430 SmaI CCC'GGG Cuts at: 1872 3757 1872 Size: 1885 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2061 2230 2534 3380 4534 5435 Size: 197 926 118 169 304 846 1154 901 Cuts at: 5435 5697 820 Size: 262 1553 Fragments arranged by size: 1553 1154 926 901 846 304 262 197 169 118 SnaBI TAC'GTA Cuts at: 493 4221 493 Size: 3728 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6430 SphI G_CATG'C Cuts at: 1132 3788 1132 Size: 2656 3774 SspI AAT'ATT Cuts at: 5 52 4354 5 Size: 47 4302 2081 Fragments arranged by size: 4302 2081 47 StuI AGG'CCT Cuts at: 1517 3176 1517 Size: 1659 4771 StyI C'CwwG_G Cuts at: 513 1065 2745 3313 3351 3684 3869 513 Size: 552 1680 568 38 333 185 3074 Fragments arranged by size: 3074 1680 568 552 333 185 38 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3026 4457 4474 4627 4812 5257 325 Size: 1255 1446 1431 17 153 185 445 1498 Fragments arranged by size: 1498 1446 1431 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2919 4676 6388 Size: 274 80 33 51 1443 942 1757 1712 Cuts at: 6388 96 Size: 138 Fragments arranged by size: 1757 1712 1443 942 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6390 6422 6390 Size: 32 6398 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6430 XbaI T'CTAG_A Cuts at: 3766 3766 Size: 6430 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2743 3529 2743 Size: 786 5644 XhoI C'TCGA_G Cuts at: 1943 2061 1943 Size: 118 6312 XmaI C'CCGG_G Cuts at: 1870 3755 1870 Size: 1885 4545 XmnI GAAnn'nnTTC Cuts at: 4559 4559 Size: 6430 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4238 276 Size: 53 83 186 936 2704 2468 Fragments arranged by size: 2704 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FalI FspAI KasI NarI NruI NsiI PacI PmlI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI