Restriction map for
pFN21AB6519
(Circular) (Six-base) MAPSORT of: pFN21AB6519.seq Check: 4229 from: 1 to: 5791
pFN21AB6519 5791 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3601 278
Size: 53 83 186 936 2065 2468
Fragments arranged by size:
2468 2065 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2888 3134 1937
Size: 951 246 4594
Fragments arranged by size:
4594 951 246
Acc65I G'GTAC_C
Cuts at: 3112 3112
Size: 5791
AclI AA'CG_TT
Cuts at: 1563 3918 4291 1563
Size: 2355 373 3063
Fragments arranged by size:
3063 2355 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2334 3854 5238 5506 1632
Size: 702 1520 1384 268 1917
Fragments arranged by size:
1917 1520 1384 702 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5594
AflIII A'CryG_T
Cuts at: 1203 4690 5667 1203
Size: 3487 977 1327
Fragments arranged by size:
3487 1327 977
AgeI A'CCGG_T
Cuts at: 4622 4622
Size: 5791
AhdI GACnn_n'nnGTC
Cuts at: 4520 4520
Size: 5791
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5791
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5791
AlwNI CAG_nnn'CTG
Cuts at: 3534 5106 3534
Size: 1572 4219
ApaLI G'TGCA_C
Cuts at: 3848 5004 3848
Size: 1156 4635
ApoI r'AATT_y
Cuts at: 1493 3100 3326 3478 5587 1493
Size: 1607 226 152 2109 1697
Fragments arranged by size:
2109 1697 1607 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4345 160
Size: 872 3313 1606
Fragments arranged by size:
3313 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5791
AvaI C'yCGr_G
Cuts at: 1870 1943 2403 3116 1870
Size: 73 460 713 4545
Fragments arranged by size:
4545 713 460 73
BamHI G'GATC_C
Cuts at: 3121 3488 3121
Size: 367 5424
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3112 4567 618
Size: 325 217 627 1325 1455 1842
Fragments arranged by size:
1842 1455 1325 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2176 2247 2404 3110 1391
Size: 785 71 157 706 4072
Fragments arranged by size:
4072 785 706 157 71
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3056 928
Size: 971 1157 3663
Fragments arranged by size:
3663 1157 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3982 4016 1903
Size: 34 2045 34 3678
Fragments arranged by size:
3678 2045 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2890 3683 4893 2890
Size: 793 1210 3788
Fragments arranged by size:
3788 1210 793
BclI T'GATC_A
Cuts at: 1548 3052 1548
Size: 1504 4287
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2028 2129 4402
Size: 107 122 71 631 605 356 101 2273
Cuts at: 4402 136
Size: 1525
Fragments arranged by size:
2273 1525 631 605 356 122 107 101
71
BglII A'GATC_T
Cuts at: 2377 5786 2377
Size: 3409 2382
BlpI GC'TnA_GC
Cuts at: 3208 3208
Size: 5791
Bme1580I G_kGCm'C
Cuts at: 1790 3852 5008 1790
Size: 2062 1156 2573
Fragments arranged by size:
2573 2062 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5791
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2167 3067 3522
Size: 416 53 83 399 24 750 900 455
Cuts at: 3522 4480 442
Size: 958 1753
Fragments arranged by size:
1753 958 900 750 455 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5791
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2119 2119
Size: 5791
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2045 2742 4451 1239
Size: 806 697 1709 2579
Fragments arranged by size:
2579 1709 806 697
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3916 4781 5079 3916
Size: 865 298 4628
Fragments arranged by size:
4628 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4454 882
Size: 617 2955 2219
Fragments arranged by size:
2955 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3582 493
Size: 711 2378 2702
Fragments arranged by size:
2702 2378 711
BsaBI GATnn'nnATC
Cuts at: 2793 5785 2793
Size: 2992 2799
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3598 3980 4615 275
Size: 53 83 186 936 2065 382 635 1451
Fragments arranged by size:
2065 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2197 4224 4622 4896 5043 5454 2197
Size: 2027 398 274 147 411 2534
Fragments arranged by size:
2534 2027 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5566
Fragments arranged by size:
5566 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2219 1186
Size: 1033 4758
BseYI C'CCAG_C
Cuts at: 4994 5490 4994
Size: 496 5295
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5218
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2109 3241 4002 5030 1540
Size: 275 186 108 1132 761 1028 2301
Fragments arranged by size:
2301 1132 1028 761 275 186 108
BsiHKAI G_wGCw'C
Cuts at: 2693 3852 3937 5008 2693
Size: 1159 85 1071 3476
Fragments arranged by size:
3476 1159 1071 85
BsmI GAATG_Cn'
Cuts at: 3302 3395 3302
Size: 93 5698
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2176 2247 2404 2693 3110 3852 3937
Size: 399 386 71 157 289 417 742 85
Cuts at: 3937 5008 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 417 399 386 289 157
85 71
BspEI T'CCGG_A
Cuts at: 5454 5454
Size: 5791
BspHI T'CATG_A
Cuts at: 2524 3680 5410 2524
Size: 1156 1730 2905
Fragments arranged by size:
2905 1730 1156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3146 844
Size: 2302 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3678 1394
Size: 429 1855 3507
Fragments arranged by size:
3507 1855 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2878 4286 4460 65
Size: 1183 1630 1408 174 1396
Fragments arranged by size:
1630 1408 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4435 4622 1700
Size: 110 2625 187 2869
Fragments arranged by size:
2869 2625 187 110
BsrGI T'GTAC_A
Cuts at: 96 2430 96
Size: 2334 3457
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5791
BssSI C'ACGA_G
Cuts at: 3851 4863 3851
Size: 1012 4779
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5791
BstBI TT'CG_AA
Cuts at: 4595 4595
Size: 5791
BstEII G'GTnAC_C
Cuts at: 1184 2812 1184
Size: 1628 4163
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5791
BstYI r'GATC_y
Cuts at: 1972 2377 3121 3488 3873 3890 5342 5428 5440
Size: 405 744 367 385 17 1452 86 12
Cuts at: 5440 5786 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 744 405 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2889 2889
Size: 5791
Bsu36I CC'TnA_GG
Cuts at: 2064 2064
Size: 5791
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4300
Fragments arranged by size:
4300 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2133 487
Size: 683 56 907 4145
Fragments arranged by size:
4145 907 683 56
BtsI GCAGTG_nn'
Cuts at: 2133 3307 4101 4121 2133
Size: 1174 794 20 3803
Fragments arranged by size:
3803 1174 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5791
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5756
DraI TTT'AAA
Cuts at: 2739 3095 2739
Size: 356 5435
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4798 809
Size: 507 3482 1802
Fragments arranged by size:
3482 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 3238 4127
Size: 54 1425 50 275 18 315 1093 889
Cuts at: 4127 5756 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1093 889 315 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3238 1537
Size: 275 1426 4090
Fragments arranged by size:
4090 1426 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2046 2486 2973 3721 1353
Size: 693 440 487 748 3423
Fragments arranged by size:
3423 748 693 487 440
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2060 4378 4752 4898 387
Size: 1064 138 471 2318 374 146 1280
Fragments arranged by size:
2318 1280 1064 471 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2334 2742 3854 4451 5238 5506
Size: 393 413 289 408 1112 597 787 268
Cuts at: 5506 1239
Size: 1524
Fragments arranged by size:
1524 1112 787 597 413 408 393 289
268
EcoRI G'AATT_C
Cuts at: 3100 3478 3100
Size: 378 5413
FspI TGC'GCA
Cuts at: 4297 4297
Size: 5791
HaeII r_GCGC'y
Cuts at: 1678 2214 4938 1678
Size: 536 2724 2531
Fragments arranged by size:
2724 2531 536
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4432 4464 4506 4538 1373
Size: 32 135 32 2860 32 42 32 2626
Fragments arranged by size:
2860 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2260 3135 3381 669
Size: 988 281 322 875 246 3079
Fragments arranged by size:
3079 988 875 322 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5791
HpaI GTT'AAC
Cuts at: 3381 3381
Size: 5791
KpnI G_GTAC'C
Cuts at: 3116 3116
Size: 5791
MfeI C'AATT_G
Cuts at: 3390 3390
Size: 5791
MluI A'CGCG_T
Cuts at: 5667 5667
Size: 5791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4905 5089 1305
Size: 3600 184 2007
Fragments arranged by size:
3600 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3969
Fragments arranged by size:
3969 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2643 3749 4108 4267 518
Size: 648 1477 1106 359 159 2042
Fragments arranged by size:
2042 1477 1106 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3207 3884 5032 5277 1606
Size: 1601 677 1148 245 2120
Fragments arranged by size:
2120 1601 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4300
Fragments arranged by size:
4300 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5791
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5791
NotI GC'GGCC_GC
Cuts at: 3238 3238
Size: 5791
NspI r_CATG'y
Cuts at: 1132 1229 2344 2642 2705 3149 4694 1132
Size: 97 1115 298 63 444 1545 2229
Fragments arranged by size:
2229 1545 1115 444 298 97 63
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5791
PciI A'CATG_T
Cuts at: 4690 4690
Size: 5791
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5791
PmeI GTTT'AAAC
Cuts at: 3095 3095
Size: 5791
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3860 3892 5373 5405 3860
Size: 32 1481 32 4246
Fragments arranged by size:
4246 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5791
PsiI TTA'TAA
Cuts at: 3361 3361
Size: 5791
PspXI vC'TCGA_Gb
Cuts at: 2403 2403
Size: 5791
PstI C_TGCA'G
Cuts at: 830 3143 830
Size: 2313 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5791
SalI G'TCGA_C
Cuts at: 1936 3133 1936
Size: 1197 4594
SbfI CC_TGCA'GG
Cuts at: 3143 3143
Size: 5791
ScaI AGT'ACT
Cuts at: 4039 5751 4039
Size: 1712 4079
SfcI C'TryA_G
Cuts at: 826 1045 2453 2769 3139 4274 4955 5146 826
Size: 219 1408 316 370 1135 681 191 1471
Fragments arranged by size:
1471 1408 1135 681 370 316 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5791
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5791
SmaI CCC'GGG
Cuts at: 1872 3118 1872
Size: 1246 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2403 3895 4796 5058 820
Size: 197 926 460 1492 901 262 1553
Fragments arranged by size:
1553 1492 926 901 460 262 197
SnaBI TAC'GTA
Cuts at: 493 3582 493
Size: 3089 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5791
SphI G_CATG'C
Cuts at: 1132 2705 3149 1132
Size: 1573 444 3774
Fragments arranged by size:
3774 1573 444
SspI AAT'ATT
Cuts at: 5 52 2365 2958 3087 3715 5
Size: 47 2313 593 129 628 2081
Fragments arranged by size:
2313 2081 628 593 129 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5791
StyI C'CwwG_G
Cuts at: 513 1065 2004 2214 3230 513
Size: 552 939 210 1016 3074
Fragments arranged by size:
3074 1016 939 552 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2095 3818 3835 3988 4173 4618 325
Size: 1255 515 1723 17 153 185 445 1498
Fragments arranged by size:
1723 1498 1255 515 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2430 4037 5749
Size: 274 80 33 51 1443 453 1607 1712
Cuts at: 5749 96
Size: 138
Fragments arranged by size:
1712 1607 1443 453 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5751 5783 5751
Size: 32 5759
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5791
XbaI T'CTAG_A
Cuts at: 3127 3127
Size: 5791
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2842 2842
Size: 5791
XhoI C'TCGA_G
Cuts at: 1943 2403 1943
Size: 460 5331
XmaI C'CCGG_G
Cuts at: 1870 3116 1870
Size: 1246 4545
XmnI GAAnn'nnTTC
Cuts at: 2463 3920 2463
Size: 1457 4334
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3599 276
Size: 53 83 186 936 2065 2468
Fragments arranged by size:
2468 2065 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI