(Circular) (Six-base) MAPSORT of: pFN21AB4714.seq Check: 4547 from: 1 to: 7327 pFN21AB4714 7327 bp With 182 enzymes: SgfI * October 20, 2009 10:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2508 3461 4558 2508 Size: 953 1097 5277 Fragments arranged by size: 5277 1097 953 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5137 278 Size: 53 83 186 936 3601 2468 Fragments arranged by size: 3601 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4379 4602 4670 1937 Size: 2442 223 68 4594 Fragments arranged by size: 4594 2442 223 68 Acc65I G'GTAC_C Cuts at: 4648 4648 Size: 7327 AclI AA'CG_TT Cuts at: 1563 4367 5454 5827 1563 Size: 2804 1087 373 3063 Fragments arranged by size: 3063 2804 1087 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2525 3563 5390 6774 7042 1632 Size: 893 1038 1827 1384 268 1917 Fragments arranged by size: 1917 1827 1384 1038 893 268 AfeI AGC'GCT Cuts at: 2429 2531 2429 Size: 102 7225 AflII C'TTAA_G Cuts at: 820 1017 2672 3050 820 Size: 197 1655 378 5097 Fragments arranged by size: 5097 1655 378 197 AflIII A'CryG_T Cuts at: 1203 4482 6226 7203 1203 Size: 3279 1744 977 1327 Fragments arranged by size: 3279 1744 1327 977 AgeI A'CCGG_T Cuts at: 6158 6158 Size: 7327 AhdI GACnn_n'nnGTC Cuts at: 3733 6056 3733 Size: 2323 5004 AleI CACnn'nnGTG Cuts at: 1166 3319 4130 4160 4440 1166 Size: 2153 811 30 280 4053 Fragments arranged by size: 4053 2153 811 280 30 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2429 1184 Size: 1245 6082 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3329 3361 4352 4384 3329 Size: 32 991 32 6272 Fragments arranged by size: 6272 991 32 32 AlwNI CAG_nnn'CTG Cuts at: 3476 5070 6642 3476 Size: 1594 1572 4161 Fragments arranged by size: 4161 1594 1572 ApaI G_GGCC'C Cuts at: 2372 3396 3888 2372 Size: 1024 492 5811 Fragments arranged by size: 5811 1024 492 ApaLI G'TGCA_C Cuts at: 5384 6540 5384 Size: 1156 6171 ApoI r'AATT_y Cuts at: 1493 2015 3125 4349 4636 4862 5014 7123 1493 Size: 522 1110 1224 287 226 152 2109 1697 Fragments arranged by size: 2109 1697 1224 1110 522 287 226 152 AseI AT'TA_AT Cuts at: 160 1032 5881 160 Size: 872 4849 1606 Fragments arranged by size: 4849 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7327 AvaI C'yCGr_G Cuts at: 1870 1943 4112 4652 1870 Size: 73 2169 540 4545 Fragments arranged by size: 4545 2169 540 73 BamHI G'GATC_C Cuts at: 4657 5024 4657 Size: 367 6960 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2458 3754 4648 6103 618 Size: 325 217 627 671 1296 894 1455 1842 Fragments arranged by size: 1842 1455 1296 894 671 627 325 217 BanII G_rGCy'C Cuts at: 1391 2372 2386 2601 3396 3505 3688 3888 3912 Size: 981 14 215 795 109 183 200 24 Cuts at: 3912 4053 4646 1391 Size: 141 593 4072 Fragments arranged by size: 4072 981 795 593 215 200 183 141 109 24 14 BbeI G_GCGC'C Cuts at: 3758 3758 Size: 7327 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2361 2481 4155 928 Size: 971 462 120 1674 4100 Fragments arranged by size: 4100 1674 971 462 120 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2370 2404 5518 5552 1903 Size: 34 433 34 3114 34 3678 Fragments arranged by size: 3678 3114 433 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5219 6429 5219 Size: 1210 6117 BclI T'GATC_A Cuts at: 1548 2646 4288 1548 Size: 1098 1642 4587 Fragments arranged by size: 4587 1642 1098 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2819 3032 5938 Size: 107 122 71 631 605 1147 213 2906 Cuts at: 5938 136 Size: 1525 Fragments arranged by size: 2906 1525 1147 631 605 213 122 107 71 BglII A'GATC_T Cuts at: 3093 3837 4267 7322 3093 Size: 744 430 3055 3098 Fragments arranged by size: 3098 3055 744 430 BlpI GC'TnA_GC Cuts at: 4744 4744 Size: 7327 Bme1580I G_kGCm'C Cuts at: 1790 2140 2372 2463 3160 3396 3744 3888 5388 Size: 350 232 91 697 236 348 144 1500 Cuts at: 5388 6544 1790 Size: 1156 2573 Fragments arranged by size: 2573 1500 1156 697 350 348 236 232 144 91 BmgBI CAC'GTC Cuts at: 1318 2521 1318 Size: 1203 6124 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2355 2457 3519 Size: 416 53 83 399 24 938 102 1062 Cuts at: 3519 4230 5058 6016 442 Size: 711 828 958 1753 Fragments arranged by size: 1753 1062 958 938 828 711 416 399 102 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7327 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2072 2455 2864 3011 3629 3698 5987 1239 Size: 833 383 409 147 618 69 2289 2579 Fragments arranged by size: 2579 2289 833 618 409 383 147 69 Bpu10I CC'TnA_GC Cuts at: 2547 3906 2547 Size: 1359 5968 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2090 3716 5452 6317 6615 2090 Size: 1626 1736 865 298 2802 Fragments arranged by size: 2802 1736 1626 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5990 882 Size: 617 4491 2219 Fragments arranged by size: 4491 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4081 5118 493 Size: 711 2877 1037 2702 Fragments arranged by size: 2877 2702 1037 711 BsaBI GATnn'nnATC Cuts at: 7321 7321 Size: 7327 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3755 5134 5516 6151 Size: 53 83 186 936 2222 1379 382 635 Cuts at: 6151 275 Size: 1451 Fragments arranged by size: 2222 1451 1379 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5760 6158 6432 6579 6990 5760 Size: 398 274 147 411 6097 Fragments arranged by size: 6097 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2583 2613 4352 4382 1178 Size: 30 165 30 1180 30 1739 30 4123 Fragments arranged by size: 4123 1739 1180 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2048 2744 3088 3473 4604 1186 Size: 862 696 344 385 1131 3909 Fragments arranged by size: 3909 1131 862 696 385 344 BseYI C'CCAG_C Cuts at: 2532 6530 7026 2532 Size: 3998 496 2833 Fragments arranged by size: 3998 2833 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2530 3937 3982 4061 4153 1163 Size: 573 794 1407 45 79 92 4337 Fragments arranged by size: 4337 1407 794 573 92 79 45 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2205 2376 3079 4777 5538 6566 Size: 275 186 204 171 703 1698 761 1028 Cuts at: 6566 1540 Size: 2301 Fragments arranged by size: 2301 1698 1028 761 703 275 204 186 171 BsiHKAI G_wGCw'C Cuts at: 2184 2880 3487 4074 5388 5473 6544 2184 Size: 696 607 587 1314 85 1071 2967 Fragments arranged by size: 2967 1314 1071 696 607 587 85 BsmI GAATG_Cn' Cuts at: 4437 4838 4931 4437 Size: 401 93 6833 Fragments arranged by size: 6833 401 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3926 3926 Size: 7327 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2140 2184 2372 2386 2463 2601 2880 Size: 399 350 44 188 14 77 138 279 Cuts at: 2880 3160 3396 3487 3505 3688 3744 3888 3912 Size: 280 236 91 18 183 56 144 24 Cuts at: 3912 4053 4074 4646 5388 5473 6544 1391 Size: 141 21 572 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 572 399 350 280 279 236 188 183 144 141 138 91 85 77 56 44 24 21 18 14 BspEI T'CCGG_A Cuts at: 6990 6990 Size: 7327 BspHI T'CATG_A Cuts at: 3084 4305 4516 5216 6946 3084 Size: 1221 211 700 1730 3465 Fragments arranged by size: 3465 1730 1221 700 211 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2508 3461 4127 4558 4682 844 Size: 1664 953 666 431 124 3489 Fragments arranged by size: 3489 1664 953 666 431 124 BsrBI CCG'CTC Cuts at: 1394 1823 5214 1394 Size: 429 3391 3507 Fragments arranged by size: 3507 3391 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2295 3021 5822 5996 65 Size: 1183 1047 726 2801 174 1396 Fragments arranged by size: 2801 1396 1183 1047 726 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5971 6158 1700 Size: 110 4161 187 2869 Fragments arranged by size: 4161 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7327 BssHII G'CGCG_C Cuts at: 1925 3247 1925 Size: 1322 6005 BssSI C'ACGA_G Cuts at: 2483 2792 5387 6399 2483 Size: 309 2595 1012 3411 Fragments arranged by size: 3411 2595 1012 309 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7327 BstBI TT'CG_AA Cuts at: 4320 6131 4320 Size: 1811 5516 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7327 BstXI CCAn_nnnn'nTGG Cuts at: 2857 3532 3979 4162 4585 2857 Size: 675 447 183 423 5599 Fragments arranged by size: 5599 675 447 423 183 BstYI r'GATC_y Cuts at: 1972 2986 3093 3837 4267 4657 5024 5409 5426 Size: 1014 107 744 430 390 367 385 17 Cuts at: 5426 6878 6964 6976 7322 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1014 744 430 390 385 367 346 107 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2193 3750 3759 3874 3912 513 Size: 552 1128 1557 9 115 38 3928 Fragments arranged by size: 3928 1557 1128 552 115 38 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6588 Fragments arranged by size: 6588 683 56 BtsI GCAGTG_nn' Cuts at: 2851 3867 4136 4843 5637 5657 2851 Size: 1016 269 707 794 20 4521 Fragments arranged by size: 4521 1016 794 707 269 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7327 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7292 DraI TTT'AAA Cuts at: 4631 4631 Size: 7327 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3310 6334 809 Size: 507 1994 3024 1802 Fragments arranged by size: 3024 1994 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2373 2709 2820 Size: 54 1425 50 275 18 543 336 111 Cuts at: 2820 3033 3357 3747 4774 5663 7292 8 Size: 213 324 390 1027 889 1629 43 Fragments arranged by size: 1629 1425 1027 889 543 390 336 324 275 213 111 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2373 4774 1537 Size: 275 561 2401 4090 Fragments arranged by size: 4090 2401 561 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2069 2721 3219 3276 3507 3539 3896 4392 Size: 716 652 498 57 231 32 357 496 Cuts at: 4392 5257 1353 Size: 865 3423 Fragments arranged by size: 3423 865 716 652 498 496 357 231 57 32 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5914 6288 6434 387 Size: 1064 138 4325 374 146 1280 Fragments arranged by size: 4325 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 2455 2525 2864 3011 3563 3629 Size: 393 440 383 70 339 147 552 66 Cuts at: 3629 3698 5390 5987 6774 7042 1239 Size: 69 1692 597 787 268 1524 Fragments arranged by size: 1692 1524 787 597 552 440 393 383 339 268 147 70 69 66 EcoNI CCTnn'n_nnAGG Cuts at: 2939 3383 2939 Size: 444 6883 EcoO109I rG'GnC_Cy Cuts at: 2368 3392 3625 3885 4116 2368 Size: 1024 233 260 231 5579 Fragments arranged by size: 5579 1024 260 233 231 EcoRI G'AATT_C Cuts at: 3125 4636 5014 3125 Size: 1511 378 5438 Fragments arranged by size: 5438 1511 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2530 2698 2530 Size: 168 7159 FspI TGC'GCA Cuts at: 2783 5833 2783 Size: 3050 4277 HaeII r_GCGC'y Cuts at: 1678 2431 2533 3758 6474 1678 Size: 753 102 1225 2716 2531 Fragments arranged by size: 2716 2531 1225 753 102 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2021 2053 2146 2178 2543 Size: 32 135 32 449 32 93 32 365 Cuts at: 2543 2575 3391 3423 3719 3751 5968 6000 6042 Size: 32 816 32 296 32 2217 32 42 Cuts at: 6042 6074 1373 Size: 32 2626 Fragments arranged by size: 2626 2217 816 449 365 296 135 93 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4671 4917 669 Size: 988 281 2733 246 3079 Fragments arranged by size: 3079 2733 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7327 HpaI GTT'AAC Cuts at: 4917 4917 Size: 7327 KasI G'GCGC_C Cuts at: 3754 3754 Size: 7327 KpnI G_GTAC'C Cuts at: 4652 4652 Size: 7327 MfeI C'AATT_G Cuts at: 4926 4926 Size: 7327 MluI A'CGCG_T Cuts at: 7203 7203 Size: 7327 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2076 2240 6441 6625 1305 Size: 771 164 4201 184 2007 Fragments arranged by size: 4201 2007 771 184 164 MscI TGG'CCA Cuts at: 10 64 1489 1832 2711 3035 3359 3749 10 Size: 54 1425 343 879 324 324 390 3588 Fragments arranged by size: 3588 1425 879 390 343 324 324 54 MslI CAynn'nnrTG Cuts at: 518 1166 3319 4130 4160 4440 4526 5285 5644 Size: 648 2153 811 30 280 86 759 359 Cuts at: 5644 5803 518 Size: 159 2042 Fragments arranged by size: 2153 2042 811 759 648 359 280 159 86 30 MspA1I CmG'CkG Cuts at: 1606 2722 2966 3876 3951 4160 4179 4743 5420 Size: 1116 244 910 75 209 19 564 677 Cuts at: 5420 6568 6813 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1116 910 677 564 245 244 209 75 19 NarI GG'CG_CC Cuts at: 3755 3755 Size: 7327 NcoI C'CATG_G Cuts at: 513 1065 3759 513 Size: 552 2694 4081 Fragments arranged by size: 4081 2694 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7327 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7327 NotI GC'GGCC_GC Cuts at: 4774 4774 Size: 7327 NspI r_CATG'y Cuts at: 1132 1229 4685 6230 1132 Size: 97 3456 1545 2229 Fragments arranged by size: 3456 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7327 PciI A'CATG_T Cuts at: 6226 6226 Size: 7327 PflMI CCAn_nnn'nTGG Cuts at: 1108 4024 1108 Size: 2916 4411 PfoI T'CCnGG_A Cuts at: 2088 3606 3843 2088 Size: 1518 237 5572 Fragments arranged by size: 5572 1518 237 PmeI GTTT'AAAC Cuts at: 4631 4631 Size: 7327 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4352 4384 5396 5428 6909 6941 4352 Size: 32 1012 32 1481 32 4738 Fragments arranged by size: 4738 1481 1012 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4116 4116 Size: 7327 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7327 PsiI TTA'TAA Cuts at: 4897 4897 Size: 7327 PspOMI G'GGCC_C Cuts at: 2368 3392 3884 2368 Size: 1024 492 5811 Fragments arranged by size: 5811 1024 492 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3766 3798 3766 Size: 32 7295 PstI C_TGCA'G Cuts at: 830 2418 2846 2894 3693 4679 830 Size: 1588 428 48 799 986 3478 Fragments arranged by size: 3478 1588 986 799 428 48 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7327 PvuII CAG'CTG Cuts at: 2722 2966 4160 4179 2722 Size: 244 1194 19 5870 Fragments arranged by size: 5870 1194 244 19 SacII CC_GC'GG Cuts at: 3877 3877 Size: 7327 SalI G'TCGA_C Cuts at: 1936 4669 1936 Size: 2733 4594 SbfI CC_TGCA'GG Cuts at: 4679 4679 Size: 7327 ScaI AGT'ACT Cuts at: 5575 7287 5575 Size: 1712 5615 SexAI A'CCwGG_T Cuts at: 2997 2997 Size: 7327 SfcI C'TryA_G Cuts at: 826 1045 2414 2842 2890 3689 4281 4675 5810 Size: 219 1369 428 48 799 592 394 1135 Cuts at: 5810 6491 6682 826 Size: 681 191 1471 Fragments arranged by size: 1471 1369 1135 799 681 592 428 394 219 191 48 SfoI GGC'GCC Cuts at: 3756 3756 Size: 7327 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7327 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7327 SmaI CCC'GGG Cuts at: 1872 4114 4654 1872 Size: 2242 540 4545 Fragments arranged by size: 4545 2242 540 SmlI C'TyrA_G Cuts at: 820 1017 1943 2069 2672 3050 3731 5431 6332 Size: 197 926 126 603 378 681 1700 901 Cuts at: 6332 6594 820 Size: 262 1553 Fragments arranged by size: 1700 1553 926 901 681 603 378 262 197 126 SnaBI TAC'GTA Cuts at: 493 5118 493 Size: 4625 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7327 SphI G_CATG'C Cuts at: 1132 4685 1132 Size: 3553 3774 SspI AAT'ATT Cuts at: 5 52 4414 5251 5 Size: 47 4362 837 2081 Fragments arranged by size: 4362 2081 837 47 StuI AGG'CCT Cuts at: 1517 2210 4061 1517 Size: 693 1851 4783 Fragments arranged by size: 4783 1851 693 StyI C'CwwG_G Cuts at: 513 1065 2901 3759 4023 4766 513 Size: 552 1836 858 264 743 3074 Fragments arranged by size: 3074 1836 858 743 552 264 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2219 2524 2914 3597 4493 5354 5371 Size: 1255 639 305 390 683 896 861 17 Cuts at: 5371 5524 5709 6154 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 896 861 683 639 445 390 305 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3417 3696 5573 Size: 274 80 33 51 1443 1440 279 1877 Cuts at: 5573 7285 96 Size: 1712 138 Fragments arranged by size: 1877 1712 1443 1440 279 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2583 2615 7287 7319 2583 Size: 32 4672 32 2591 Fragments arranged by size: 4672 2591 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2522 3661 1316 Size: 1206 1139 4982 Fragments arranged by size: 4982 1206 1139 XbaI T'CTAG_A Cuts at: 3703 4663 3703 Size: 960 6367 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2719 3673 3757 2719 Size: 954 84 6289 Fragments arranged by size: 6289 954 84 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7327 XmaI C'CCGG_G Cuts at: 1870 4112 4652 1870 Size: 2242 540 4545 Fragments arranged by size: 4545 2242 540 XmnI GAAnn'nnTTC Cuts at: 2015 5456 2015 Size: 3441 3886 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5135 276 Size: 53 83 186 936 3601 2468 Fragments arranged by size: 3601 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SacI SanDI SapI SfiI SrfI SwaI