(Circular) (Six-base) MAPSORT of: pFN21AB4124.seq Check: 9273 from: 1 to: 7684 pFN21AB4124 7684 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5494 278 Size: 53 83 186 936 3958 2468 Fragments arranged by size: 3958 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3494 5027 1937 Size: 1557 1533 4594 Fragments arranged by size: 4594 1557 1533 Acc65I G'GTAC_C Cuts at: 2022 5005 2022 Size: 2983 4701 AclI AA'CG_TT Cuts at: 1563 5811 6184 1563 Size: 4248 373 3063 Fragments arranged by size: 4248 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2861 3483 5747 7131 7399 1632 Size: 1229 622 2264 1384 268 1917 Fragments arranged by size: 2264 1917 1384 1229 622 268 AflII C'TTAA_G Cuts at: 820 1017 3274 820 Size: 197 2257 5230 Fragments arranged by size: 5230 2257 197 AflIII A'CryG_T Cuts at: 1203 2702 4506 6583 7560 1203 Size: 1499 1804 2077 977 1327 Fragments arranged by size: 2077 1804 1499 1327 977 AgeI A'CCGG_T Cuts at: 6515 6515 Size: 7684 AhdI GACnn_n'nnGTC Cuts at: 6413 6413 Size: 7684 AleI CACnn'nnGTG Cuts at: 1166 2513 2754 1166 Size: 1347 241 6096 Fragments arranged by size: 6096 1347 241 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2772 1184 Size: 1588 6096 AlwNI CAG_nnn'CTG Cuts at: 2663 4355 5427 6999 2663 Size: 1692 1072 1572 3348 Fragments arranged by size: 3348 1692 1572 1072 ApaLI G'TGCA_C Cuts at: 5741 6897 5741 Size: 1156 6528 ApoI r'AATT_y Cuts at: 1493 2312 2672 3791 3917 4380 4424 4634 4653 Size: 819 360 1119 126 463 44 210 19 Cuts at: 4653 4993 5219 5371 7480 1493 Size: 340 226 152 2109 1697 Fragments arranged by size: 2109 1697 1119 819 463 360 340 226 210 152 126 44 19 AseI AT'TA_AT Cuts at: 160 1032 3510 6238 160 Size: 872 2478 2728 1606 Fragments arranged by size: 2728 2478 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7684 AvaI C'yCGr_G Cuts at: 1870 1943 4329 5009 1870 Size: 73 2386 680 4545 Fragments arranged by size: 4545 2386 680 73 BamHI G'GATC_C Cuts at: 5014 5381 5014 Size: 367 7317 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2022 3800 5005 6460 618 Size: 325 217 627 235 1778 1205 1455 1842 Fragments arranged by size: 1842 1778 1455 1205 627 325 235 217 BanII G_rGCy'C Cuts at: 1391 2241 2962 4144 5003 1391 Size: 850 721 1182 859 4072 Fragments arranged by size: 4072 1182 859 850 721 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2330 2853 928 Size: 971 431 523 5759 Fragments arranged by size: 5759 971 523 431 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3315 3349 5875 5909 1903 Size: 34 1378 34 2526 34 3678 Fragments arranged by size: 3678 2526 1378 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3158 5576 6786 3158 Size: 2418 1210 4056 Fragments arranged by size: 4056 2418 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7684 BfrBI ATG'CAT Cuts at: 4151 4151 Size: 7684 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4578 6295 136 Size: 107 122 71 631 605 2906 1717 1525 Fragments arranged by size: 2906 1717 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2872 7679 2872 Size: 4807 2877 BlpI GC'TnA_GC Cuts at: 5101 5101 Size: 7684 Bme1580I G_kGCm'C Cuts at: 1790 2781 5745 6901 1790 Size: 991 2964 1156 2573 Fragments arranged by size: 2964 2573 1156 991 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7684 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2360 5415 6373 Size: 416 53 83 399 24 943 3055 958 Cuts at: 6373 442 Size: 1753 Fragments arranged by size: 3055 1753 958 943 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3224 1056 Size: 2168 5516 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2444 2444 Size: 7684 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2364 2911 3619 6344 1239 Size: 1125 547 708 2725 2579 Fragments arranged by size: 2725 2579 1125 708 547 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2853 5809 6674 6972 2853 Size: 2956 865 298 3565 Fragments arranged by size: 3565 2956 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6347 882 Size: 617 4848 2219 Fragments arranged by size: 4848 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5475 493 Size: 711 4271 2702 Fragments arranged by size: 4271 2702 711 BsaBI GATnn'nnATC Cuts at: 7678 7678 Size: 7684 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5491 5873 6508 275 Size: 53 83 186 936 3958 382 635 1451 Fragments arranged by size: 3958 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4419 6117 6515 6789 6936 7347 4419 Size: 1698 398 274 147 411 4756 Fragments arranged by size: 4756 1698 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2980 3010 1178 Size: 30 165 30 1577 30 5852 Fragments arranged by size: 5852 1577 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2914 1186 Size: 1728 5956 BseYI C'CCAG_C Cuts at: 3174 3680 4573 6887 7383 3174 Size: 506 893 2314 496 3475 Fragments arranged by size: 3475 2314 893 506 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2736 2778 2988 3139 3930 1163 Size: 573 1000 42 210 151 791 4917 Fragments arranged by size: 4917 1000 791 573 210 151 42 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5134 5895 6923 1540 Size: 275 186 3133 761 1028 2301 Fragments arranged by size: 3133 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2885 4948 5745 5830 6901 2885 Size: 2063 797 85 1071 3668 Fragments arranged by size: 3668 2063 1071 797 85 BsmI GAATG_Cn' Cuts at: 4206 4929 4974 5195 5288 4206 Size: 723 45 221 93 6602 Fragments arranged by size: 6602 723 221 93 45 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2241 2781 2885 2962 4144 4948 5003 Size: 399 451 540 104 77 1182 804 55 Cuts at: 5003 5745 5830 6901 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1182 1071 804 742 540 451 399 104 85 77 55 BspEI T'CCGG_A Cuts at: 7347 7347 Size: 7684 BspHI T'CATG_A Cuts at: 4110 4920 5573 7303 4110 Size: 810 653 1730 4491 Fragments arranged by size: 4491 1730 810 653 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3225 5039 844 Size: 2381 1814 3489 Fragments arranged by size: 3489 2381 1814 BsrBI CCG'CTC Cuts at: 1394 1823 5571 1394 Size: 429 3748 3507 Fragments arranged by size: 3748 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2290 2448 3660 6179 6353 65 Size: 1183 1042 158 1212 2519 174 1396 Fragments arranged by size: 2519 1396 1212 1183 1042 174 158 BsrFI r'CCGG_y Cuts at: 1700 1810 6328 6515 1700 Size: 110 4518 187 2869 Fragments arranged by size: 4518 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7684 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7684 BssSI C'ACGA_G Cuts at: 5744 6756 5744 Size: 1012 6672 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7684 BstBI TT'CG_AA Cuts at: 4672 6488 4672 Size: 1816 5868 BstEII G'GTnAC_C Cuts at: 1184 2477 1184 Size: 1293 6391 BstXI CCAn_nnnn'nTGG Cuts at: 3182 3694 4392 3182 Size: 512 698 6474 Fragments arranged by size: 6474 698 512 BstYI r'GATC_y Cuts at: 1972 2872 5014 5381 5766 5783 7235 7321 7333 Size: 900 2142 367 385 17 1452 86 12 Cuts at: 7333 7679 1972 Size: 346 1977 Fragments arranged by size: 2142 1977 1452 900 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2321 2321 Size: 7684 BtgI C'CryG_G Cuts at: 513 1065 2004 2845 4563 513 Size: 552 939 841 1718 3634 Fragments arranged by size: 3634 1718 939 841 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3780 487 Size: 683 56 2554 4391 Fragments arranged by size: 4391 2554 683 56 BtsI GCAGTG_nn' Cuts at: 2719 2749 2761 3940 5200 5994 6014 2719 Size: 30 12 1179 1260 794 20 4389 Fragments arranged by size: 4389 1260 1179 794 30 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7684 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7649 DraI TTT'AAA Cuts at: 3790 4988 3790 Size: 1198 6486 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6691 809 Size: 507 5375 1802 Fragments arranged by size: 5375 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2842 3937 5131 Size: 54 1425 50 275 18 1012 1095 1194 Cuts at: 5131 6020 7649 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1194 1095 1012 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5131 1537 Size: 275 3319 4090 Fragments arranged by size: 4090 3319 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3765 4734 5614 1353 Size: 2412 969 880 3423 Fragments arranged by size: 3423 2412 969 880 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6271 6645 6791 387 Size: 1064 138 4682 374 146 1280 Fragments arranged by size: 4682 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2364 2861 2911 3483 3619 5747 6344 Size: 393 732 497 50 572 136 2128 597 Cuts at: 6344 7131 7399 1239 Size: 787 268 1524 Fragments arranged by size: 2128 1524 787 732 597 572 497 393 268 136 50 EcoO109I rG'GnC_Cy Cuts at: 3835 4261 3835 Size: 426 7258 EcoRI G'AATT_C Cuts at: 3917 4653 4993 5371 3917 Size: 736 340 378 6230 Fragments arranged by size: 6230 736 378 340 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2498 2498 Size: 7684 FspI TGC'GCA Cuts at: 6190 6190 Size: 7684 HaeII r_GCGC'y Cuts at: 1678 6831 1678 Size: 5153 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2137 2169 6325 6357 6399 Size: 32 135 32 565 32 4156 32 42 Cuts at: 6399 6431 1373 Size: 32 2626 Fragments arranged by size: 4156 2626 565 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4288 5028 5274 669 Size: 988 281 2350 740 246 3079 Fragments arranged by size: 3079 2350 988 740 281 246 HindIII A'AGCT_T Cuts at: 748 2501 748 Size: 1753 5931 HpaI GTT'AAC Cuts at: 5274 5274 Size: 7684 KpnI G_GTAC'C Cuts at: 2026 5009 2026 Size: 2983 4701 MfeI C'AATT_G Cuts at: 5283 5283 Size: 7684 MluI A'CGCG_T Cuts at: 7560 7560 Size: 7684 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2118 2918 4578 4630 4753 6798 6982 1305 Size: 813 800 1660 52 123 2045 184 2007 Fragments arranged by size: 2045 2007 1660 813 800 184 123 52 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5862 Fragments arranged by size: 5862 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2513 2754 3209 3411 3665 3695 4065 Size: 648 1347 241 455 202 254 30 370 Cuts at: 4065 4962 4979 5642 6001 6160 518 Size: 897 17 663 359 159 2042 Fragments arranged by size: 2042 1347 897 663 648 455 370 359 254 241 202 159 30 17 MspA1I CmG'CkG Cuts at: 1606 3373 3629 4577 4829 5100 5777 6925 7170 Size: 1767 256 948 252 271 677 1148 245 Cuts at: 7170 1606 Size: 2120 Fragments arranged by size: 2120 1767 1148 948 677 271 256 252 245 NcoI C'CATG_G Cuts at: 513 1065 2004 4563 513 Size: 552 939 2559 3634 Fragments arranged by size: 3634 2559 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7684 NheI G'CTAG_C Cuts at: 1052 3220 1052 Size: 2168 5516 NotI GC'GGCC_GC Cuts at: 5131 5131 Size: 7684 NsiI A_TGCA'T Cuts at: 4153 4153 Size: 7684 NspI r_CATG'y Cuts at: 1132 1229 2706 2988 3172 4510 5042 6587 1132 Size: 97 1477 282 184 1338 532 1545 2229 Fragments arranged by size: 2229 1545 1477 1338 532 282 184 97 PasI CC'CwG_GG Cuts at: 1348 4699 1348 Size: 3351 4333 PciI A'CATG_T Cuts at: 2702 4506 6583 2702 Size: 1804 2077 3803 Fragments arranged by size: 3803 2077 1804 PflMI CCAn_nnn'nTGG Cuts at: 1108 2162 2166 2233 1108 Size: 1054 4 67 6559 Fragments arranged by size: 6559 1054 67 4 PfoI T'CCnGG_A Cuts at: 2341 2341 Size: 7684 PmeI GTTT'AAAC Cuts at: 4988 4988 Size: 7684 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5753 5785 7266 7298 5753 Size: 32 1481 32 6139 Fragments arranged by size: 6139 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3835 4261 3835 Size: 426 7258 PshAI GACnn'nnGTC Cuts at: 1613 2533 2705 1613 Size: 920 172 6592 Fragments arranged by size: 6592 920 172 PsiI TTA'TAA Cuts at: 4526 5254 4526 Size: 728 6956 PstI C_TGCA'G Cuts at: 830 2458 2756 3070 3372 3935 5036 830 Size: 1628 298 314 302 563 1101 3478 Fragments arranged by size: 3478 1628 1101 563 314 302 298 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7684 PvuII CAG'CTG Cuts at: 3629 4577 4829 3629 Size: 948 252 6484 Fragments arranged by size: 6484 948 252 SalI G'TCGA_C Cuts at: 1936 5026 1936 Size: 3090 4594 SapI GCTCTTCn'nnn_ Cuts at: 3765 3765 Size: 7684 SbfI CC_TGCA'GG Cuts at: 5036 5036 Size: 7684 ScaI AGT'ACT Cuts at: 2103 2349 3366 5932 7644 2103 Size: 246 1017 2566 1712 2143 Fragments arranged by size: 2566 2143 1712 1017 246 SexAI A'CCwGG_T Cuts at: 2217 2217 Size: 7684 SfcI C'TryA_G Cuts at: 826 1045 2454 2752 3066 3097 3368 3618 3624 Size: 219 1409 298 314 31 271 250 6 Cuts at: 3624 3675 3931 5032 6167 6848 7039 826 Size: 51 256 1101 1135 681 191 1471 Fragments arranged by size: 1471 1409 1135 1101 681 314 298 271 256 250 219 191 51 31 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7684 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7684 SmaI CCC'GGG Cuts at: 1872 5011 1872 Size: 3139 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2868 3274 5788 6689 6951 820 Size: 197 926 925 406 2514 901 262 1553 Fragments arranged by size: 2514 1553 926 925 901 406 262 197 SnaBI TAC'GTA Cuts at: 493 5475 493 Size: 4982 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7684 SphI G_CATG'C Cuts at: 1132 2988 5042 1132 Size: 1856 2054 3774 Fragments arranged by size: 3774 2054 1856 SspI AAT'ATT Cuts at: 5 52 3105 4279 5608 5 Size: 47 3053 1174 1329 2081 Fragments arranged by size: 3053 2081 1329 1174 47 StuI AGG'CCT Cuts at: 1517 2995 1517 Size: 1478 6206 StyI C'CwwG_G Cuts at: 513 1065 2004 2473 2482 3864 4563 5123 513 Size: 552 939 469 9 1382 699 560 3074 Fragments arranged by size: 3074 1382 939 699 560 552 469 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2154 2173 4576 5711 5728 5881 6066 Size: 1255 574 19 2403 1135 17 153 185 Cuts at: 6066 6511 325 Size: 445 1498 Fragments arranged by size: 2403 1498 1255 1135 574 445 185 153 19 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2101 2347 2379 Size: 274 80 33 51 1443 124 246 32 Cuts at: 2379 2604 3364 5930 7642 96 Size: 225 760 2566 1712 138 Fragments arranged by size: 2566 1712 1443 760 274 246 225 138 124 80 51 33 32 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2980 3012 7644 7676 2980 Size: 32 4632 32 2988 Fragments arranged by size: 4632 2988 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7684 XbaI T'CTAG_A Cuts at: 4657 5020 4657 Size: 363 7321 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2163 2480 3046 2163 Size: 317 566 6801 Fragments arranged by size: 6801 566 317 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7684 XmaI C'CCGG_G Cuts at: 1870 5009 1870 Size: 3139 4545 XmnI GAAnn'nnTTC Cuts at: 3141 5813 3141 Size: 2672 5012 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5492 276 Size: 53 83 186 936 3958 2468 Fragments arranged by size: 3958 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI