(Circular) (Six-base) MAPSORT of: pFN21AB3289.seq Check: 4530 from: 1 to: 7114 pFN21AB3289 7114 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4924 278 Size: 53 83 186 936 3388 2468 Fragments arranged by size: 3388 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4457 1937 Size: 2520 4594 Acc65I G'GTAC_C Cuts at: 4435 4435 Size: 7114 AclI AA'CG_TT Cuts at: 1563 5241 5614 1563 Size: 3678 373 3063 Fragments arranged by size: 3678 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2454 2770 3103 3122 3187 3374 3523 3607 Size: 822 316 333 19 65 187 149 84 Cuts at: 3607 3710 3859 4111 4195 4279 5177 6561 6829 Size: 103 149 252 84 84 898 1384 268 Cuts at: 6829 1632 Size: 1917 Fragments arranged by size: 1917 1384 898 822 333 316 268 252 187 149 149 103 84 84 84 65 19 AflII C'TTAA_G Cuts at: 820 1017 2924 820 Size: 197 1907 5010 Fragments arranged by size: 5010 1907 197 AflIII A'CryG_T Cuts at: 1203 2846 2908 6013 6990 1203 Size: 1643 62 3105 977 1327 Fragments arranged by size: 3105 1643 1327 977 62 AgeI A'CCGG_T Cuts at: 5945 5945 Size: 7114 AhdI GACnn_n'nnGTC Cuts at: 5843 5843 Size: 7114 AleI CACnn'nnGTG Cuts at: 1166 2661 1166 Size: 1495 5619 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2340 1184 Size: 1156 5958 AlwNI CAG_nnn'CTG Cuts at: 4857 6429 4857 Size: 1572 5542 ApaI G_GGCC'C Cuts at: 2084 2084 Size: 7114 ApaLI G'TGCA_C Cuts at: 3969 4079 5171 6327 3969 Size: 110 1092 1156 4756 Fragments arranged by size: 4756 1156 1092 110 ApoI r'AATT_y Cuts at: 1493 2233 2427 2571 2948 3211 3547 3813 4423 Size: 740 194 144 377 263 336 266 610 Cuts at: 4423 4649 4801 6910 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 740 610 377 336 266 263 226 194 152 144 AseI AT'TA_AT Cuts at: 160 1032 5668 160 Size: 872 4636 1606 Fragments arranged by size: 4636 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7114 AvaI C'yCGr_G Cuts at: 1870 1943 4439 1870 Size: 73 2496 4545 Fragments arranged by size: 4545 2496 73 BamHI G'GATC_C Cuts at: 3825 4444 4811 3825 Size: 619 367 6128 Fragments arranged by size: 6128 619 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4435 5890 618 Size: 325 217 627 2648 1455 1842 Fragments arranged by size: 2648 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2084 3719 4433 1391 Size: 693 1635 714 4072 Fragments arranged by size: 4072 1635 714 693 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2230 3380 3865 4136 4201 4212 928 Size: 971 331 1150 485 271 65 11 3830 Fragments arranged by size: 3830 1150 971 485 331 271 65 11 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5305 5339 1903 Size: 34 3368 34 3678 Fragments arranged by size: 3678 3368 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5006 6216 5006 Size: 1210 5904 BclI T'GATC_A Cuts at: 1548 2358 2722 1548 Size: 810 364 5940 Fragments arranged by size: 5940 810 364 BfrBI ATG'CAT Cuts at: 3188 4069 3188 Size: 881 6233 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5725 136 Size: 107 122 71 631 605 4053 1525 Fragments arranged by size: 4053 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3805 7109 3805 Size: 3304 3810 BlpI GC'TnA_GC Cuts at: 4531 4531 Size: 7114 Bme1580I G_kGCm'C Cuts at: 1790 2084 3973 4083 5175 6331 1790 Size: 294 1889 110 1092 1156 2573 Fragments arranged by size: 2573 1889 1156 1092 294 110 BmgBI CAC'GTC Cuts at: 1318 2041 1318 Size: 723 6391 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2490 2876 3171 Size: 416 53 83 399 24 1073 386 295 Cuts at: 3171 4263 4845 5803 442 Size: 1092 582 958 1753 Fragments arranged by size: 1753 1092 1073 958 582 416 399 386 295 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7114 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2782 3435 3519 4023 5774 1239 Size: 1543 653 84 504 1751 2579 Fragments arranged by size: 2579 1751 1543 653 504 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2524 4123 5239 6104 6402 2524 Size: 1599 1116 865 298 3236 Fragments arranged by size: 3236 1599 1116 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5777 882 Size: 617 4278 2219 Fragments arranged by size: 4278 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2992 4905 493 Size: 711 1788 1913 2702 Fragments arranged by size: 2702 1913 1788 711 BsaBI GATnn'nnATC Cuts at: 2811 7108 2811 Size: 4297 2817 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4921 5303 5938 275 Size: 53 83 186 936 3388 382 635 1451 Fragments arranged by size: 3388 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5547 5945 6219 6366 6777 5547 Size: 398 274 147 411 5884 Fragments arranged by size: 5884 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6889 Fragments arranged by size: 6889 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2075 3116 3284 3704 3872 1186 Size: 889 1041 168 420 168 4428 Fragments arranged by size: 4428 1041 889 420 168 168 BseYI C'CCAG_C Cuts at: 2077 6317 6813 2077 Size: 4240 496 2378 Fragments arranged by size: 4240 2378 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6541 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3964 4564 5325 6353 1540 Size: 275 186 1963 600 761 1028 2301 Fragments arranged by size: 2301 1963 1028 761 600 275 186 BsiHKAI G_wGCw'C Cuts at: 3719 3973 4083 5175 5260 6331 3719 Size: 254 110 1092 85 1071 4502 Fragments arranged by size: 4502 1092 1071 254 110 85 BsmI GAATG_Cn' Cuts at: 4625 4718 4625 Size: 93 7021 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2084 3719 3973 4083 4433 5175 5260 Size: 399 294 1635 254 110 350 742 85 Cuts at: 5260 6331 1391 Size: 1071 2174 Fragments arranged by size: 2174 1635 1071 742 399 350 294 254 110 85 BspEI T'CCGG_A Cuts at: 6777 6777 Size: 7114 BspHI T'CATG_A Cuts at: 2638 5003 6733 2638 Size: 2365 1730 3019 Fragments arranged by size: 3019 2365 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2142 4469 844 Size: 1298 2327 3489 Fragments arranged by size: 3489 2327 1298 BsrBI CCG'CTC Cuts at: 1394 1823 5001 1394 Size: 429 3178 3507 Fragments arranged by size: 3507 3178 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3026 3061 3526 5609 5783 65 Size: 1183 1778 35 465 2083 174 1396 Fragments arranged by size: 2083 1778 1396 1183 465 174 35 BsrFI r'CCGG_y Cuts at: 1700 1810 5758 5945 1700 Size: 110 3948 187 2869 Fragments arranged by size: 3948 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7114 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7114 BssSI C'ACGA_G Cuts at: 5174 6186 5174 Size: 1012 6102 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7114 BstBI TT'CG_AA Cuts at: 5918 5918 Size: 7114 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7114 BstYI r'GATC_y Cuts at: 1972 3805 3825 4444 4811 5196 5213 6665 6751 Size: 1833 20 619 367 385 17 1452 86 Cuts at: 6751 6763 7109 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1833 1452 619 385 367 346 86 20 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6562 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6375 Fragments arranged by size: 6375 683 56 BtsI GCAGTG_nn' Cuts at: 2031 4630 5424 5444 2031 Size: 2599 794 20 3701 Fragments arranged by size: 3701 2599 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7114 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3508 3543 529 Size: 35 2944 35 4100 Fragments arranged by size: 4100 2944 35 35 DraI TTT'AAA Cuts at: 4418 4418 Size: 7114 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2416 6121 809 Size: 507 1100 3705 1802 Fragments arranged by size: 3705 1802 1100 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4561 5450 7079 Size: 54 1425 50 275 18 2731 889 1629 Cuts at: 7079 8 Size: 43 Fragments arranged by size: 2731 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4561 1537 Size: 275 2749 4090 Fragments arranged by size: 4090 2749 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2286 3527 3768 3947 5044 1353 Size: 933 1241 241 179 1097 3423 Fragments arranged by size: 3423 1241 1097 933 241 179 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5701 6075 6221 387 Size: 1064 138 4112 374 146 1280 Fragments arranged by size: 4112 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3717 3717 Size: 7114 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2454 2770 2782 3103 3122 3187 3374 Size: 393 822 316 12 321 19 65 187 Cuts at: 3374 3435 3519 3523 3607 3710 3859 4023 4111 Size: 61 84 4 84 103 149 164 88 Cuts at: 4111 4195 4279 5177 5774 6561 6829 1239 Size: 84 84 898 597 787 268 1524 Fragments arranged by size: 1524 898 822 787 597 393 321 316 268 187 164 149 103 88 84 84 84 84 65 61 19 12 4 EcoO109I rG'GnC_Cy Cuts at: 2081 3114 2081 Size: 1033 6081 EcoRI G'AATT_C Cuts at: 2948 3211 3547 4423 4801 2948 Size: 263 336 876 378 5261 Fragments arranged by size: 5261 876 378 336 263 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2312 4069 2312 Size: 1757 5357 FspI TGC'GCA Cuts at: 5620 5620 Size: 7114 HaeII r_GCGC'y Cuts at: 1678 2964 6261 1678 Size: 1286 3297 2531 Fragments arranged by size: 3297 2531 1286 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2267 2299 2620 2652 2707 Size: 32 135 32 695 32 321 32 55 Cuts at: 2707 2739 3190 3222 3454 3486 3946 3958 3978 Size: 32 451 32 232 32 460 12 20 Cuts at: 3978 3990 5755 5787 5829 5861 1373 Size: 12 1765 32 42 32 2626 Fragments arranged by size: 2626 1765 695 460 451 321 232 135 55 42 32 32 32 32 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 4458 4704 669 Size: 988 281 2520 246 3079 Fragments arranged by size: 3079 2520 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7114 HpaI GTT'AAC Cuts at: 4704 4704 Size: 7114 KpnI G_GTAC'C Cuts at: 4439 4439 Size: 7114 MfeI C'AATT_G Cuts at: 4713 4713 Size: 7114 MluI A'CGCG_T Cuts at: 6990 6990 Size: 7114 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2606 3188 3944 6228 6412 1305 Size: 1301 582 756 2284 184 2007 Fragments arranged by size: 2284 2007 1301 756 582 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5292 Fragments arranged by size: 5292 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2640 2661 3010 3178 3262 3430 Size: 648 843 631 21 349 168 84 168 Cuts at: 3430 3514 3750 3766 3934 4018 4102 4158 4186 Size: 84 236 16 168 84 84 56 28 Cuts at: 4186 4338 5072 5431 5590 518 Size: 152 734 359 159 2042 Fragments arranged by size: 2042 843 734 648 631 359 349 236 168 168 168 159 152 84 84 84 84 56 28 21 16 MspA1I CmG'CkG Cuts at: 1606 3739 4530 5207 6355 6600 1606 Size: 2133 791 677 1148 245 2120 Fragments arranged by size: 2133 2120 1148 791 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6562 NdeI CA'TA_TG Cuts at: 387 3431 387 Size: 3044 4070 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7114 NotI GC'GGCC_GC Cuts at: 4561 4561 Size: 7114 NsiI A_TGCA'T Cuts at: 3190 4071 3190 Size: 881 6233 NspI r_CATG'y Cuts at: 1132 1229 2850 2912 3192 4069 4472 6017 1132 Size: 97 1621 62 280 877 403 1545 2229 Fragments arranged by size: 2229 1621 1545 877 403 280 97 62 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7114 PciI A'CATG_T Cuts at: 2846 2908 6013 2846 Size: 62 3105 3947 Fragments arranged by size: 3947 3105 62 PflMI CCAn_nnn'nTGG Cuts at: 1108 2625 1108 Size: 1517 5597 PmeI GTTT'AAAC Cuts at: 4418 4418 Size: 7114 PmlI CAC'GTG Cuts at: 2992 2992 Size: 7114 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5183 5215 6696 6728 5183 Size: 32 1481 32 5569 Fragments arranged by size: 5569 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7114 PsiI TTA'TAA Cuts at: 3093 4269 4684 3093 Size: 1176 415 5523 Fragments arranged by size: 5523 1176 415 PspOMI G'GGCC_C Cuts at: 2080 2080 Size: 7114 PstI C_TGCA'G Cuts at: 830 4466 830 Size: 3636 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7114 PvuII CAG'CTG Cuts at: 3739 3739 Size: 7114 SacI G_AGCT'C Cuts at: 3719 3719 Size: 7114 SalI G'TCGA_C Cuts at: 1936 4456 1936 Size: 2520 4594 SapI GCTCTTCn'nnn_ Cuts at: 2286 3527 2286 Size: 1241 5873 SbfI CC_TGCA'GG Cuts at: 4466 4466 Size: 7114 ScaI AGT'ACT Cuts at: 4124 5362 7074 4124 Size: 1238 1712 4164 Fragments arranged by size: 4164 1712 1238 SexAI A'CCwGG_T Cuts at: 2339 2400 2339 Size: 61 7053 SfcI C'TryA_G Cuts at: 826 1045 2178 2969 3820 4229 4462 5597 6278 Size: 219 1133 791 851 409 233 1135 681 Cuts at: 6278 6469 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1133 851 791 681 409 233 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7114 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7114 SmaI CCC'GGG Cuts at: 1872 4441 1872 Size: 2569 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2503 2924 4138 5218 6119 6381 Size: 197 926 560 421 1214 1080 901 262 Cuts at: 6381 820 Size: 1553 Fragments arranged by size: 1553 1214 1080 926 901 560 421 262 197 SnaBI TAC'GTA Cuts at: 493 4905 493 Size: 4412 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7114 SphI G_CATG'C Cuts at: 1132 4472 1132 Size: 3340 3774 SspI AAT'ATT Cuts at: 5 52 5038 5 Size: 47 4986 2081 Fragments arranged by size: 4986 2081 47 StuI AGG'CCT Cuts at: 1517 3128 3884 4136 1517 Size: 1611 756 252 4495 Fragments arranged by size: 4495 1611 756 252 StyI C'CwwG_G Cuts at: 513 1065 4553 513 Size: 552 3488 3074 Fragments arranged by size: 3488 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3950 4206 5141 5158 5311 5496 5941 Size: 1255 2370 256 935 17 153 185 445 Cuts at: 5941 325 Size: 1498 Fragments arranged by size: 2370 1498 1255 935 445 256 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2653 2905 3434 Size: 274 80 33 51 1443 676 252 529 Cuts at: 3434 4122 5360 7072 96 Size: 688 1238 1712 138 Fragments arranged by size: 1712 1443 1238 688 676 529 274 252 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7074 7106 7074 Size: 32 7082 Tth111I GACn'n_nGTC Cuts at: 1316 2542 1316 Size: 1226 5888 XbaI T'CTAG_A Cuts at: 4450 4450 Size: 7114 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7114 XmaI C'CCGG_G Cuts at: 1870 4439 1870 Size: 2569 4545 XmnI GAAnn'nnTTC Cuts at: 2012 2531 3032 3173 3536 3620 3872 3956 4040 Size: 519 501 141 363 84 252 84 84 Cuts at: 4040 5243 2012 Size: 1203 3883 Fragments arranged by size: 3883 1203 519 501 363 252 141 84 84 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4922 276 Size: 53 83 186 936 3388 2468 Fragments arranged by size: 3388 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI