(Circular) (Six-base) MAPSORT of: pFN21AB3214.seq Check: 8502 from: 1 to: 7228 pFN21AB3214 7228 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5038 278 Size: 53 83 186 936 3502 2468 Fragments arranged by size: 3502 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4571 1937 Size: 2634 4594 Acc65I G'GTAC_C Cuts at: 3508 4549 3508 Size: 1041 6187 AclI AA'CG_TT Cuts at: 1563 5355 5728 1563 Size: 3792 373 3063 Fragments arranged by size: 3792 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2365 5291 6675 6943 1632 Size: 733 2926 1384 268 1917 Fragments arranged by size: 2926 1917 1384 733 268 AfeI AGC'GCT Cuts at: 3313 3989 3313 Size: 676 6552 AflII C'TTAA_G Cuts at: 820 1017 3025 820 Size: 197 2008 5023 Fragments arranged by size: 5023 2008 197 AflIII A'CryG_T Cuts at: 1203 2673 6127 7104 1203 Size: 1470 3454 977 1327 Fragments arranged by size: 3454 1470 1327 977 AgeI A'CCGG_T Cuts at: 6059 6059 Size: 7228 AhdI GACnn_n'nnGTC Cuts at: 3808 5957 3808 Size: 2149 5079 AleI CACnn'nnGTG Cuts at: 1166 3335 1166 Size: 2169 5059 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3111 3353 4284 1184 Size: 1927 242 931 4128 Fragments arranged by size: 4128 1927 931 242 AlwNI CAG_nnn'CTG Cuts at: 2200 2404 3570 4180 4971 6543 2200 Size: 204 1166 610 791 1572 2885 Fragments arranged by size: 2885 1572 1166 791 610 204 ApaI G_GGCC'C Cuts at: 2155 2155 Size: 7228 ApaLI G'TGCA_C Cuts at: 5285 6441 5285 Size: 1156 6072 ApoI r'AATT_y Cuts at: 1493 4021 4537 4763 4915 7024 1493 Size: 2528 516 226 152 2109 1697 Fragments arranged by size: 2528 2109 1697 516 226 152 AseI AT'TA_AT Cuts at: 160 1032 5782 160 Size: 872 4750 1606 Fragments arranged by size: 4750 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7228 AvaI C'yCGr_G Cuts at: 1870 1943 2627 4163 4405 4553 1870 Size: 73 684 1536 242 148 4545 Fragments arranged by size: 4545 1536 684 242 148 73 BamHI G'GATC_C Cuts at: 4558 4925 4558 Size: 367 6861 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3388 3508 4348 4549 6004 Size: 325 217 627 1601 120 840 201 1455 Cuts at: 6004 618 Size: 1842 Fragments arranged by size: 1842 1601 1455 840 627 325 217 201 120 BanII G_rGCy'C Cuts at: 1391 2155 2571 2663 3611 4163 4547 1391 Size: 764 416 92 948 552 384 4072 Fragments arranged by size: 4072 948 764 552 416 384 92 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6257 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2462 2496 5419 5453 1903 Size: 34 525 34 2923 34 3678 Fragments arranged by size: 3678 2923 525 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5120 6330 5120 Size: 1210 6018 BclI T'GATC_A Cuts at: 1548 1548 Size: 7228 BfrBI ATG'CAT Cuts at: 2738 4098 2738 Size: 1360 5868 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2159 2537 5839 Size: 107 122 71 631 605 487 378 3302 Cuts at: 5839 136 Size: 1525 Fragments arranged by size: 3302 1525 631 605 487 378 122 107 71 BglII A'GATC_T Cuts at: 3874 7223 3874 Size: 3349 3879 BlpI GC'TnA_GC Cuts at: 4257 4645 4257 Size: 388 6840 Bme1580I G_kGCm'C Cuts at: 1790 2155 2643 4305 4351 5289 6445 1790 Size: 365 488 1662 46 938 1156 2573 Fragments arranged by size: 2573 1662 1156 938 488 365 46 BmgBI CAC'GTC Cuts at: 1318 2119 2638 1318 Size: 801 519 5908 Fragments arranged by size: 5908 801 519 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2838 3069 4959 Size: 416 53 83 399 24 1421 231 1890 Cuts at: 4959 5917 442 Size: 958 1753 Fragments arranged by size: 1890 1753 1421 958 416 399 231 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7228 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3658 4446 3658 Size: 788 6440 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2387 2907 3011 3292 3754 4213 5888 1239 Size: 1148 520 104 281 462 459 1675 2579 Fragments arranged by size: 2579 1675 1148 520 462 459 281 104 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2786 5353 6218 6516 2786 Size: 2567 865 298 3498 Fragments arranged by size: 3498 2567 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3036 3539 5891 882 Size: 617 1537 503 2352 2219 Fragments arranged by size: 2352 2219 1537 617 503 BsaAI yAC'GTr Cuts at: 493 1204 3193 5019 493 Size: 711 1989 1826 2702 Fragments arranged by size: 2702 1989 1826 711 BsaBI GATnn'nnATC Cuts at: 7222 7222 Size: 7228 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2901 5035 5417 6052 Size: 53 83 186 936 1368 2134 382 635 Cuts at: 6052 275 Size: 1451 Fragments arranged by size: 2134 1451 1368 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2934 3285 3782 5661 6059 6333 6480 6891 2934 Size: 351 497 1879 398 274 147 411 3271 Fragments arranged by size: 3271 1879 497 411 398 351 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4106 4136 1178 Size: 30 165 30 2703 30 4270 Fragments arranged by size: 4270 2703 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2258 2504 2507 2519 2522 3295 3304 3631 Size: 1072 246 3 12 3 773 9 327 Cuts at: 3631 3634 4399 4422 1186 Size: 3 765 23 3992 Fragments arranged by size: 3992 1072 773 765 327 246 23 12 9 3 3 3 BseYI C'CCAG_C Cuts at: 2397 2945 2971 2975 3428 3984 4352 6431 6927 Size: 548 26 4 453 556 368 2079 496 Cuts at: 6927 2397 Size: 2698 Fragments arranged by size: 2698 2079 556 548 496 453 368 26 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2047 3842 1163 Size: 573 311 1795 4549 Fragments arranged by size: 4549 1795 573 311 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4678 5439 6467 1540 Size: 275 186 2677 761 1028 2301 Fragments arranged by size: 2677 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2663 3332 5289 5374 6445 2663 Size: 669 1957 85 1071 3446 Fragments arranged by size: 3446 1957 1071 669 85 BsmI GAATG_Cn' Cuts at: 3340 4739 4832 3340 Size: 1399 93 5736 Fragments arranged by size: 5736 1399 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2504 2519 2504 Size: 15 7213 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2155 2571 2643 2663 3332 3611 4163 Size: 399 365 416 72 20 669 279 552 Cuts at: 4163 4305 4351 4547 5289 5374 6445 1391 Size: 142 46 196 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 669 552 416 399 365 279 196 142 85 72 46 20 BspEI T'CCGG_A Cuts at: 3782 6891 3782 Size: 3109 4119 BspHI T'CATG_A Cuts at: 3946 5117 6847 3946 Size: 1171 1730 4327 Fragments arranged by size: 4327 1730 1171 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4583 844 Size: 3739 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2915 3603 5115 1394 Size: 429 1092 688 1512 3507 Fragments arranged by size: 3507 1512 1092 688 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4266 5723 5897 65 Size: 1183 3018 1457 174 1396 Fragments arranged by size: 3018 1457 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3385 3413 5872 6059 1700 Size: 110 1575 28 2459 187 2869 Fragments arranged by size: 2869 2459 1575 187 110 28 BsrGI T'GTAC_A Cuts at: 96 3717 4493 96 Size: 3621 776 2831 Fragments arranged by size: 3621 2831 776 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7228 BssSI C'ACGA_G Cuts at: 2462 5288 6300 2462 Size: 2826 1012 3390 Fragments arranged by size: 3390 2826 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7228 BstBI TT'CG_AA Cuts at: 6032 6032 Size: 7228 BstEII G'GTnAC_C Cuts at: 1184 3140 1184 Size: 1956 5272 BstXI CCAn_nnnn'nTGG Cuts at: 2011 3007 2011 Size: 996 6232 BstYI r'GATC_y Cuts at: 1972 2996 3874 4170 4558 4925 5310 5327 6779 Size: 1024 878 296 388 367 385 17 1452 Cuts at: 6779 6865 6877 7223 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1024 878 388 385 367 346 296 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2241 2755 3236 2241 Size: 514 481 6233 Fragments arranged by size: 6233 514 481 BtgI C'CryG_G Cuts at: 513 1065 2964 3222 3448 4209 4221 4461 513 Size: 552 1899 258 226 761 12 240 3280 Fragments arranged by size: 3280 1899 761 552 258 240 226 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3961 487 Size: 683 56 2735 3754 Fragments arranged by size: 3754 2735 683 56 BtsI GCAGTG_nn' Cuts at: 2059 2185 2289 4293 4744 5538 5558 2059 Size: 126 104 2004 451 794 20 3729 Fragments arranged by size: 3729 2004 794 451 126 104 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7228 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7193 DraI TTT'AAA Cuts at: 4532 4532 Size: 7228 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6235 809 Size: 507 4919 1802 Fragments arranged by size: 4919 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2169 2529 3415 Size: 54 1425 50 275 18 339 360 886 Cuts at: 3415 3462 4675 5564 7193 8 Size: 47 1213 889 1629 43 Fragments arranged by size: 1629 1425 1213 889 886 360 339 275 54 50 47 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4675 1537 Size: 275 2863 4090 Fragments arranged by size: 4090 2863 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2496 2844 2856 3580 5158 1353 Size: 1143 348 12 724 1578 3423 Fragments arranged by size: 3423 1578 1143 724 348 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5815 6189 6335 387 Size: 1064 138 4226 374 146 1280 Fragments arranged by size: 4226 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2661 2661 Size: 7228 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2365 2387 2907 3011 3292 3754 4213 Size: 393 733 22 520 104 281 462 459 Cuts at: 4213 5291 5888 6675 6943 1239 Size: 1078 597 787 268 1524 Fragments arranged by size: 1524 1078 787 733 597 520 462 459 393 281 268 104 22 EcoNI CCTnn'n_nnAGG Cuts at: 3025 3168 4133 4381 3025 Size: 143 965 248 5872 Fragments arranged by size: 5872 965 248 143 EcoO109I rG'GnC_Cy Cuts at: 3242 4177 4427 3242 Size: 935 250 6043 Fragments arranged by size: 6043 935 250 EcoRI G'AATT_C Cuts at: 4537 4915 4537 Size: 378 6850 FspI TGC'GCA Cuts at: 2354 4285 5734 2354 Size: 1931 1449 3848 Fragments arranged by size: 3848 1931 1449 HaeII r_GCGC'y Cuts at: 1678 2201 2458 3315 3991 6375 1678 Size: 523 257 857 676 2384 2531 Fragments arranged by size: 2531 2384 857 676 523 257 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2304 2336 3794 3826 4106 Size: 32 135 32 732 32 1458 32 280 Cuts at: 4106 4138 5869 5901 5943 5975 1373 Size: 32 1731 32 42 32 2626 Fragments arranged by size: 2626 1731 1458 732 280 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2679 4572 4818 669 Size: 988 281 741 1893 246 3079 Fragments arranged by size: 3079 1893 988 741 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7228 HpaI GTT'AAC Cuts at: 2679 4818 2679 Size: 2139 5089 KpnI G_GTAC'C Cuts at: 3512 4553 3512 Size: 1041 6187 MfeI C'AATT_G Cuts at: 4827 4827 Size: 7228 MluI A'CGCG_T Cuts at: 2673 7104 2673 Size: 4431 2797 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2185 3231 3237 3979 4092 6342 6526 1305 Size: 880 1046 6 742 113 2250 184 2007 Fragments arranged by size: 2250 2007 1046 880 742 184 113 6 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5406 Fragments arranged by size: 5406 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2039 3335 5186 5545 5704 518 Size: 648 843 30 1296 1851 359 159 2042 Fragments arranged by size: 2042 1851 1296 843 648 359 159 30 MspA1I CmG'CkG Cuts at: 1606 2095 2272 2337 2975 3501 4644 5321 6469 Size: 489 177 65 638 526 1143 677 1148 Cuts at: 6469 6714 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1143 677 638 526 489 245 177 65 NaeI GCC'GGC Cuts at: 3415 3415 Size: 7228 NcoI C'CATG_G Cuts at: 513 1065 3222 4209 513 Size: 552 2157 987 3532 Fragments arranged by size: 3532 2157 987 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7228 NgoMIV G'CCGG_C Cuts at: 3413 3413 Size: 7228 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7228 NotI GC'GGCC_GC Cuts at: 4675 4675 Size: 7228 NsiI A_TGCA'T Cuts at: 2740 4100 2740 Size: 1360 5868 NspI r_CATG'y Cuts at: 1132 1229 2178 2359 2718 4098 4586 6131 1132 Size: 97 949 181 359 1380 488 1545 2229 Fragments arranged by size: 2229 1545 1380 949 488 359 181 97 PasI CC'CwG_GG Cuts at: 1348 2547 3658 4344 1348 Size: 1199 1111 686 4232 Fragments arranged by size: 4232 1199 1111 686 PciI A'CATG_T Cuts at: 6127 6127 Size: 7228 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7228 PfoI T'CCnGG_A Cuts at: 2775 2999 2775 Size: 224 7004 PmeI GTTT'AAAC Cuts at: 4532 4532 Size: 7228 PmlI CAC'GTG Cuts at: 3193 3193 Size: 7228 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5297 5329 6810 6842 5297 Size: 32 1481 32 5683 Fragments arranged by size: 5683 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4427 4427 Size: 7228 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7228 PsiI TTA'TAA Cuts at: 4798 4798 Size: 7228 PspOMI G'GGCC_C Cuts at: 2151 2151 Size: 7228 PstI C_TGCA'G Cuts at: 830 2394 2485 3620 4580 830 Size: 1564 91 1135 960 3478 Fragments arranged by size: 3478 1564 1135 960 91 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7228 PvuII CAG'CTG Cuts at: 2975 3501 2975 Size: 526 6702 SacI G_AGCT'C Cuts at: 2663 2663 Size: 7228 SalI G'TCGA_C Cuts at: 1936 4570 1936 Size: 2634 4594 SapI GCTCTTCn'nnn_ Cuts at: 3580 3580 Size: 7228 SbfI CC_TGCA'GG Cuts at: 4580 4580 Size: 7228 ScaI AGT'ACT Cuts at: 5476 7188 5476 Size: 1712 5516 SfcI C'TryA_G Cuts at: 826 1045 2390 2481 3616 3858 4576 5711 6392 Size: 219 1345 91 1135 242 718 1135 681 Cuts at: 6392 6583 826 Size: 191 1471 Fragments arranged by size: 1471 1345 1135 1135 718 681 242 219 191 91 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7228 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7228 SmaI CCC'GGG Cuts at: 1872 4407 4555 1872 Size: 2535 148 4545 Fragments arranged by size: 4545 2535 148 SmlI C'TyrA_G Cuts at: 820 1017 1943 2801 3025 5332 6233 6495 820 Size: 197 926 858 224 2307 901 262 1553 Fragments arranged by size: 2307 1553 926 901 858 262 224 197 SnaBI TAC'GTA Cuts at: 493 5019 493 Size: 4526 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7228 SphI G_CATG'C Cuts at: 1132 2178 2359 4098 4586 1132 Size: 1046 181 1739 488 3774 Fragments arranged by size: 3774 1739 1046 488 181 SspI AAT'ATT Cuts at: 5 52 2186 5152 5 Size: 47 2134 2966 2081 Fragments arranged by size: 2966 2134 2081 47 StuI AGG'CCT Cuts at: 1517 3440 3699 1517 Size: 1923 259 5046 Fragments arranged by size: 5046 1923 259 StyI C'CwwG_G Cuts at: 513 1065 2652 2904 3222 3540 3881 4209 4248 Size: 552 1587 252 318 318 341 328 39 Cuts at: 4248 4667 513 Size: 419 3074 Fragments arranged by size: 3074 1587 552 419 341 328 318 318 252 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3394 4241 4366 5255 5272 5425 5610 Size: 1255 1814 847 125 889 17 153 185 Cuts at: 5610 6055 325 Size: 445 1498 Fragments arranged by size: 1814 1498 1255 889 847 445 185 153 125 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3077 3717 4493 Size: 274 80 33 51 1443 1100 640 776 Cuts at: 4493 5474 7186 96 Size: 981 1712 138 Fragments arranged by size: 1712 1443 1100 981 776 640 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2432 2464 3862 3894 7188 7220 2432 Size: 32 1398 32 3294 32 2440 Fragments arranged by size: 3294 2440 1398 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7228 XbaI T'CTAG_A Cuts at: 4564 4564 Size: 7228 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2074 3220 3399 2074 Size: 1146 179 5903 Fragments arranged by size: 5903 1146 179 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7228 XmaI C'CCGG_G Cuts at: 1870 4405 4553 1870 Size: 2535 148 4545 Fragments arranged by size: 4545 2535 148 XmnI GAAnn'nnTTC Cuts at: 2144 3890 5357 2144 Size: 1746 1467 4015 Fragments arranged by size: 4015 1746 1467 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5036 276 Size: 53 83 186 936 3502 2468 Fragments arranged by size: 3502 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI NruI PacI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI