(Circular) (Six-base) MAPSORT of: pFN21AB4094.seq Check: 8313 from: 1 to: 6550 pFN21AB4094 6550 bp With 182 enzymes: SgfI * June 13, 2018 20:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3264 3264 Size: 6550 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4360 278 Size: 53 83 186 936 2824 2468 Fragments arranged by size: 2824 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2307 3893 1937 Size: 370 1586 4594 Fragments arranged by size: 4594 1586 370 Acc65I G'GTAC_C Cuts at: 2844 3871 2844 Size: 1027 5523 AclI AA'CG_TT Cuts at: 1563 2175 4677 5050 1563 Size: 612 2502 373 3063 Fragments arranged by size: 3063 2502 612 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2033 2419 3167 3627 4613 5997 6265 1632 Size: 401 386 748 460 986 1384 268 1917 Fragments arranged by size: 1917 1384 986 748 460 401 386 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6353 AflIII A'CryG_T Cuts at: 1203 2982 5449 6426 1203 Size: 1779 2467 977 1327 Fragments arranged by size: 2467 1779 1327 977 AgeI A'CCGG_T Cuts at: 5381 5381 Size: 6550 AhdI GACnn_n'nnGTC Cuts at: 5279 5279 Size: 6550 AleI CACnn'nnGTG Cuts at: 1166 3308 1166 Size: 2142 4408 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6550 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2714 2746 2714 Size: 32 6518 AlwNI CAG_nnn'CTG Cuts at: 2521 2806 4293 5865 2521 Size: 285 1487 1572 3206 Fragments arranged by size: 3206 1572 1487 285 ApaI G_GGCC'C Cuts at: 2419 2583 3467 2419 Size: 164 884 5502 Fragments arranged by size: 5502 884 164 ApaLI G'TGCA_C Cuts at: 4607 5763 4607 Size: 1156 5394 ApoI r'AATT_y Cuts at: 1493 3859 4085 4237 6346 1493 Size: 2366 226 152 2109 1697 Fragments arranged by size: 2366 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5104 160 Size: 872 4072 1606 Fragments arranged by size: 4072 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6550 AvaI C'yCGr_G Cuts at: 1870 1943 2180 2325 3444 3597 3733 3875 1870 Size: 73 237 145 1119 153 136 142 4545 Fragments arranged by size: 4545 1119 237 153 145 142 136 73 AvrII C'CTAG_G Cuts at: 3349 3349 Size: 6550 BamHI G'GATC_C Cuts at: 3880 4247 3880 Size: 367 6183 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2300 2465 2844 3206 3533 Size: 325 217 627 513 165 379 362 327 Cuts at: 3533 3628 3871 5326 618 Size: 95 243 1455 1842 Fragments arranged by size: 1842 1455 627 513 379 362 327 325 243 217 165 95 BanII G_rGCy'C Cuts at: 1391 2187 2419 2459 2583 2892 3467 3495 3869 Size: 796 232 40 124 309 575 28 374 Cuts at: 3869 1391 Size: 4072 Fragments arranged by size: 4072 796 575 374 309 232 124 40 28 BbeI G_GCGC'C Cuts at: 2304 2304 Size: 6550 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2033 3093 928 Size: 971 134 1060 4385 Fragments arranged by size: 4385 1060 971 134 BbvCI CC'TCA_GC Cuts at: 2390 2601 2941 2390 Size: 211 340 5999 Fragments arranged by size: 5999 340 211 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3829 3863 4741 4775 1903 Size: 34 1892 34 878 34 3678 Fragments arranged by size: 3678 1892 878 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4442 5652 4442 Size: 1210 5340 BclI T'GATC_A Cuts at: 1548 1548 Size: 6550 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2463 2516 2663 Size: 107 122 71 631 605 791 53 147 Cuts at: 2663 3035 3410 5161 136 Size: 372 375 1751 1525 Fragments arranged by size: 1751 1525 791 631 605 375 372 147 122 107 71 53 BglII A'GATC_T Cuts at: 2872 3096 6545 2872 Size: 224 3449 2877 Fragments arranged by size: 3449 2877 224 BlpI GC'TnA_GC Cuts at: 2667 3967 2667 Size: 1300 5250 Bme1580I G_kGCm'C Cuts at: 1790 2419 2583 3209 3467 3536 4611 5767 1790 Size: 629 164 626 258 69 1075 1156 2573 Fragments arranged by size: 2573 1156 1075 629 626 258 164 69 BmgBI CAC'GTC Cuts at: 1318 2059 1318 Size: 741 5809 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2453 4281 5239 Size: 416 53 83 399 24 1036 1828 958 Cuts at: 5239 442 Size: 1753 Fragments arranged by size: 1828 1753 1036 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6550 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2326 2801 2906 3188 3215 5210 1239 Size: 1087 475 105 282 27 1995 2579 Fragments arranged by size: 2579 1995 1087 475 282 105 27 Bpu10I CC'TnA_GC Cuts at: 2390 2601 2941 3624 2390 Size: 211 340 683 5316 Fragments arranged by size: 5316 683 340 211 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3812 4675 5540 5838 3812 Size: 863 865 298 4524 Fragments arranged by size: 4524 865 863 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2371 2531 5213 882 Size: 617 872 160 2682 2219 Fragments arranged by size: 2682 2219 872 617 160 BsaAI yAC'GTr Cuts at: 493 1204 3319 4341 493 Size: 711 2115 1022 2702 Fragments arranged by size: 2702 2115 1022 711 BsaBI GATnn'nnATC Cuts at: 6544 6544 Size: 6550 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2301 4357 4739 5374 Size: 53 83 186 936 768 2056 382 635 Cuts at: 5374 275 Size: 1451 Fragments arranged by size: 2056 1451 936 768 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2483 4983 5381 5655 5802 6213 2483 Size: 2500 398 274 147 411 2820 Fragments arranged by size: 2820 2500 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2714 2744 1178 Size: 30 165 30 1311 30 4984 Fragments arranged by size: 4984 1311 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2108 2894 3107 3194 3305 3437 3503 1186 Size: 922 786 213 87 111 132 66 4233 Fragments arranged by size: 4233 922 786 213 132 111 87 66 BseYI C'CCAG_C Cuts at: 2083 2125 2198 3324 5753 6249 2083 Size: 42 73 1126 2429 496 2384 Fragments arranged by size: 2429 2384 1126 496 73 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2750 3299 3362 3567 3794 1163 Size: 573 1014 549 63 205 227 3919 Fragments arranged by size: 3919 1014 573 549 227 205 63 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3188 3414 4000 4761 5789 1540 Size: 275 186 1187 226 586 761 1028 2301 Fragments arranged by size: 2301 1187 1028 761 586 275 226 186 BsiHKAI G_wGCw'C Cuts at: 2112 2187 3672 4611 4696 5767 2112 Size: 75 1485 939 85 1071 2895 Fragments arranged by size: 2895 1485 1071 939 85 75 BsmI GAATG_Cn' Cuts at: 4061 4154 4061 Size: 93 6457 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2112 2187 2419 2459 2583 2892 3209 Size: 399 322 75 232 40 124 309 317 Cuts at: 3209 3467 3495 3536 3672 3869 4611 4696 5767 Size: 258 28 41 136 197 742 85 1071 Cuts at: 5767 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 322 317 309 258 232 197 136 124 85 75 41 40 28 BspEI T'CCGG_A Cuts at: 6213 6213 Size: 6550 BspHI T'CATG_A Cuts at: 4439 6169 4439 Size: 1730 4820 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2772 3150 3264 3905 844 Size: 1928 378 114 641 3489 Fragments arranged by size: 3489 1928 641 378 114 BsrBI CCG'CTC Cuts at: 1394 1823 2397 2671 3184 4437 1394 Size: 429 574 274 513 1253 3507 Fragments arranged by size: 3507 1253 574 513 429 274 BsrDI GCAATG_nn' Cuts at: 65 1248 2934 3022 5045 5219 65 Size: 1183 1686 88 2023 174 1396 Fragments arranged by size: 2023 1686 1396 1183 174 88 BsrFI r'CCGG_y Cuts at: 1700 1810 2657 2901 3453 3536 5194 5381 1700 Size: 110 847 244 552 83 1658 187 2869 Fragments arranged by size: 2869 1658 847 552 244 187 110 83 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6550 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6550 BssSI C'ACGA_G Cuts at: 4610 5622 4610 Size: 1012 5538 BstAPI GCAn_nnn'nTGC Cuts at: 794 2806 3382 794 Size: 2012 576 3962 Fragments arranged by size: 3962 2012 576 BstBI TT'CG_AA Cuts at: 5354 5354 Size: 6550 BstEII G'GTnAC_C Cuts at: 1184 2605 1184 Size: 1421 5129 BstXI CCAn_nnnn'nTGG Cuts at: 3166 3166 Size: 6550 BstYI r'GATC_y Cuts at: 1972 2292 2872 3096 3880 4247 4632 4649 6101 Size: 320 580 224 784 367 385 17 1452 Cuts at: 6101 6187 6199 6545 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 784 580 385 367 346 320 224 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2857 2857 Size: 6550 BtgI C'CryG_G Cuts at: 513 1065 2004 2241 2850 3428 3467 513 Size: 552 939 237 609 578 39 3596 Fragments arranged by size: 3596 939 609 578 552 237 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2245 487 Size: 683 56 1019 4792 Fragments arranged by size: 4792 1019 683 56 BtsI GCAGTG_nn' Cuts at: 3555 4066 4860 4880 3555 Size: 511 794 20 5225 Fragments arranged by size: 5225 794 511 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6550 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3319 3354 529 Size: 35 2755 35 3725 Fragments arranged by size: 3725 2755 35 35 DraI TTT'AAA Cuts at: 3854 3854 Size: 6550 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5557 809 Size: 507 4241 1802 Fragments arranged by size: 4241 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2244 2899 2903 Size: 54 1425 50 275 18 414 655 4 Cuts at: 2903 3051 3185 3411 3538 3717 3997 4886 6515 Size: 148 134 226 127 179 280 889 1629 Cuts at: 6515 8 Size: 43 Fragments arranged by size: 1629 1425 889 655 414 280 275 226 179 148 134 127 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 3185 3411 3997 1537 Size: 275 1373 226 586 4090 Fragments arranged by size: 4090 1373 586 275 226 EarI CTCTTCn'nnn_ Cuts at: 1353 2438 4480 1353 Size: 1085 2042 3423 Fragments arranged by size: 3423 2042 1085 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3059 3214 5137 5511 5657 387 Size: 1064 138 1470 155 1923 374 146 1280 Fragments arranged by size: 1923 1470 1280 1064 374 155 146 138 EcoICRI GAG'CTC Cuts at: 2185 2185 Size: 6550 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2033 2326 2419 2801 2906 3167 3188 Size: 393 401 293 93 382 105 261 21 Cuts at: 3188 3215 3627 4613 5210 5997 6265 1239 Size: 27 412 986 597 787 268 1524 Fragments arranged by size: 1524 986 787 597 412 401 393 382 293 268 261 105 93 27 21 EcoNI CCTnn'n_nnAGG Cuts at: 2328 2552 3146 2328 Size: 224 594 5732 Fragments arranged by size: 5732 594 224 EcoO109I rG'GnC_Cy Cuts at: 2508 2518 3235 3463 3526 3557 3602 2508 Size: 10 717 228 63 31 45 5456 Fragments arranged by size: 5456 717 228 63 45 31 10 EcoRI G'AATT_C Cuts at: 3859 4237 3859 Size: 378 6172 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3127 3127 Size: 6550 FseI GG_CCGG'CC Cuts at: 2905 2905 Size: 6550 FspI TGC'GCA Cuts at: 3386 5056 3386 Size: 1670 4880 FspAI rTGC'GCAy Cuts at: 3386 3386 Size: 6550 HaeII r_GCGC'y Cuts at: 1678 2304 3741 5697 1678 Size: 626 1437 1956 2531 Fragments arranged by size: 2531 1956 1437 626 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2289 2321 2941 2973 5191 Size: 32 135 32 717 32 620 32 2218 Cuts at: 5191 5223 5265 5297 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2218 717 620 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2155 2308 3894 4140 669 Size: 988 281 217 153 1586 246 3079 Fragments arranged by size: 3079 1586 988 281 246 217 153 HindIII A'AGCT_T Cuts at: 748 748 Size: 6550 HpaI GTT'AAC Cuts at: 4140 4140 Size: 6550 KasI G'GCGC_C Cuts at: 2300 2300 Size: 6550 KpnI G_GTAC'C Cuts at: 2848 3875 2848 Size: 1027 5523 MfeI C'AATT_G Cuts at: 4149 4149 Size: 6550 MluI A'CGCG_T Cuts at: 6426 6426 Size: 6550 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2432 5664 5848 1305 Size: 1127 3232 184 2007 Fragments arranged by size: 3232 2007 1127 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3719 10 Size: 54 1425 343 1887 2841 Fragments arranged by size: 2841 1887 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3308 4508 4867 5026 518 Size: 648 2142 1200 359 159 2042 Fragments arranged by size: 2142 2042 1200 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2041 2600 2794 3966 4643 5791 6036 1606 Size: 435 559 194 1172 677 1148 245 2120 Fragments arranged by size: 2120 1172 1148 677 559 435 245 194 NaeI GCC'GGC Cuts at: 2903 3455 2903 Size: 552 5998 NarI GG'CG_CC Cuts at: 2301 2301 Size: 6550 NcoI C'CATG_G Cuts at: 513 1065 2004 2850 513 Size: 552 939 846 4213 Fragments arranged by size: 4213 939 846 552 NdeI CA'TA_TG Cuts at: 387 2378 387 Size: 1991 4559 NgoMIV G'CCGG_C Cuts at: 2901 3453 2901 Size: 552 5998 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6550 NotI GC'GGCC_GC Cuts at: 3997 3997 Size: 6550 NspI r_CATG'y Cuts at: 1132 1229 2704 2986 3908 5453 1132 Size: 97 1475 282 922 1545 2229 Fragments arranged by size: 2229 1545 1475 922 282 97 PasI CC'CwG_GG Cuts at: 1348 2521 1348 Size: 1173 5377 PciI A'CATG_T Cuts at: 2982 5449 2982 Size: 2467 4083 PflMI CCAn_nnn'nTGG Cuts at: 1108 2521 1108 Size: 1413 5137 PfoI T'CCnGG_A Cuts at: 2529 3063 3231 2529 Size: 534 168 5848 Fragments arranged by size: 5848 534 168 PmeI GTTT'AAAC Cuts at: 3854 3854 Size: 6550 PmlI CAC'GTG Cuts at: 3319 3319 Size: 6550 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2714 2746 3205 3237 4619 4651 6132 6164 2714 Size: 32 459 32 1382 32 1481 32 3100 Fragments arranged by size: 3100 1481 1382 459 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3235 3557 3235 Size: 322 6228 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6550 PsiI TTA'TAA Cuts at: 4120 4120 Size: 6550 PspOMI G'GGCC_C Cuts at: 2415 2579 3463 2415 Size: 164 884 5502 Fragments arranged by size: 5502 884 164 PspXI vC'TCGA_Gb Cuts at: 3733 3733 Size: 6550 PstI C_TGCA'G Cuts at: 830 2793 2799 3036 3902 830 Size: 1963 6 237 866 3478 Fragments arranged by size: 3478 1963 866 237 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6550 PvuII CAG'CTG Cuts at: 2600 2794 2600 Size: 194 6356 SacI G_AGCT'C Cuts at: 2187 2187 Size: 6550 SalI G'TCGA_C Cuts at: 1936 2306 3892 1936 Size: 370 1586 4594 Fragments arranged by size: 4594 1586 370 SbfI CC_TGCA'GG Cuts at: 3036 3902 3036 Size: 866 5684 ScaI AGT'ACT Cuts at: 4798 6510 4798 Size: 1712 4838 SexAI A'CCwGG_T Cuts at: 2103 2103 Size: 6550 SfcI C'TryA_G Cuts at: 826 1045 2789 2795 3032 3898 5033 5714 5905 Size: 219 1744 6 237 866 1135 681 191 Cuts at: 5905 826 Size: 1471 Fragments arranged by size: 1744 1471 1135 866 681 237 219 191 6 SfiI GGCCn_nnn'nGGCC Cuts at: 2516 3410 2516 Size: 894 5656 SfoI GGC'GCC Cuts at: 2302 2302 Size: 6550 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6550 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6550 SmaI CCC'GGG Cuts at: 1872 3446 3877 1872 Size: 1574 431 4545 Fragments arranged by size: 4545 1574 431 SmlI C'TyrA_G Cuts at: 820 1017 1943 2325 3733 3827 4654 5555 5817 Size: 197 926 382 1408 94 827 901 262 Cuts at: 5817 820 Size: 1553 Fragments arranged by size: 1553 1408 926 901 827 382 262 197 94 SnaBI TAC'GTA Cuts at: 493 4341 493 Size: 3848 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6550 SphI G_CATG'C Cuts at: 1132 3908 1132 Size: 2776 3774 SspI AAT'ATT Cuts at: 5 52 4474 5 Size: 47 4422 2081 Fragments arranged by size: 4422 2081 47 StuI AGG'CCT Cuts at: 1517 3038 3846 1517 Size: 1521 808 4221 Fragments arranged by size: 4221 1521 808 StyI C'CwwG_G Cuts at: 513 1065 2004 2118 2850 3217 3349 3989 513 Size: 552 939 114 732 367 132 640 3074 Fragments arranged by size: 3074 939 732 640 552 367 132 114 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3224 4577 4594 4747 4932 5377 325 Size: 1255 1644 1353 17 153 185 445 1498 Fragments arranged by size: 1644 1498 1353 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4796 6508 96 Size: 274 80 33 51 1443 2819 1712 138 Fragments arranged by size: 2819 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2221 2253 2411 2443 3227 3259 6510 6542 2221 Size: 32 158 32 784 32 3251 32 2229 Fragments arranged by size: 3251 2229 784 158 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6550 XbaI T'CTAG_A Cuts at: 2875 3886 2875 Size: 1011 5539 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2056 2350 2056 Size: 294 6256 XhoI C'TCGA_G Cuts at: 1943 2325 3733 1943 Size: 382 1408 4760 Fragments arranged by size: 4760 1408 382 XmaI C'CCGG_G Cuts at: 1870 3444 3875 1870 Size: 1574 431 4545 Fragments arranged by size: 4545 1574 431 XmnI GAAnn'nnTTC Cuts at: 4679 4679 Size: 6550 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4358 276 Size: 53 83 186 936 2824 2468 Fragments arranged by size: 2824 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV NruI NsiI PacI PsrI RsrII SacII SanDI SapI SrfI SwaI