Restriction map for
pFN21AB4094
(Linear) (Six-base) MAPSORT of: KIBB4094.seq Check: 8369 from: 1 to: 1845
KIBB4094.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1259 1845
Size: 1259 586
AccI GT'mk_AC
Cuts at: 0 302 1845
Size: 302 1543
Acc65I G'GTAC_C
Cuts at: 0 839 1845
Size: 839 1006
AclI AA'CG_TT
Cuts at: 0 170 1845
Size: 170 1675
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 414 1162 1622 1845
Size: 28 386 748 460 223
Fragments arranged by size:
748 460 386 223 28
AflIII A'CryG_T
Cuts at: 0 977 1845
Size: 977 868
AleI CACnn'nnGTG
Cuts at: 0 1303 1845
Size: 1303 542
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 709 741 1845
Size: 709 32 1104
Fragments arranged by size:
1104 709 32
AlwNI CAG_nnn'CTG
Cuts at: 0 516 801 1845
Size: 516 285 1044
Fragments arranged by size:
1044 516 285
ApaI G_GGCC'C
Cuts at: 0 414 578 1462 1845
Size: 414 164 884 383
Fragments arranged by size:
884 414 383 164
AvaI C'yCGr_G
Cuts at: 0 175 320 1439 1592 1728 1845
Size: 175 145 1119 153 136 117
Fragments arranged by size:
1119 175 153 145 136 117
AvrII C'CTAG_G
Cuts at: 0 1344 1845
Size: 1344 501
BanI G'GyrC_C
Cuts at: 0 295 460 839 1201 1528 1623 1845
Size: 295 165 379 362 327 95 222
Fragments arranged by size:
379 362 327 295 222 165 95
BanII G_rGCy'C
Cuts at: 0 182 414 454 578 887 1462 1490 1845
Size: 182 232 40 124 309 575 28 355
Fragments arranged by size:
575 355 309 232 182 124 40 28
BbeI G_GCGC'C
Cuts at: 0 299 1845
Size: 299 1546
BbsI GAAGACnn'nnnn_
Cuts at: 0 28 1088 1845
Size: 28 1060 757
Fragments arranged by size:
1060 757 28
BbvCI CC'TCA_GC
Cuts at: 0 385 596 936 1845
Size: 385 211 340 909
Fragments arranged by size:
909 385 340 211
BglI GCCn_nnn'nGGC
Cuts at: 0 458 511 658 1030 1405 1845
Size: 458 53 147 372 375 440
Fragments arranged by size:
458 440 375 372 147 53
BglII A'GATC_T
Cuts at: 0 867 1091 1845
Size: 867 224 754
Fragments arranged by size:
867 754 224
BlpI GC'TnA_GC
Cuts at: 0 662 1845
Size: 662 1183
Bme1580I G_kGCm'C
Cuts at: 0 414 578 1204 1462 1531 1845
Size: 414 164 626 258 69 314
Fragments arranged by size:
626 414 314 258 164 69
BmgBI CAC'GTC
Cuts at: 0 54 1845
Size: 54 1791
BmrI ACTGGGnnnn_n'
Cuts at: 0 448 1845
Size: 448 1397
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 321 796 901 1183 1210 1845
Size: 321 475 105 282 27 635
Fragments arranged by size:
635 475 321 282 105 27
Bpu10I CC'TnA_GC
Cuts at: 0 385 596 936 1619 1845
Size: 385 211 340 683 226
Fragments arranged by size:
683 385 340 226 211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1807 1845
Size: 1807 38
BsaI GGTCTCn'nnnn_
Cuts at: 0 366 526 1845
Size: 366 160 1319
Fragments arranged by size:
1319 366 160
BsaAI yAC'GTr
Cuts at: 0 1314 1845
Size: 1314 531
BsaHI Gr'CG_yC
Cuts at: 0 296 1845
Size: 296 1549
BsaWI w'CCGG_w
Cuts at: 0 478 1845
Size: 478 1367
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 709 739 1845
Size: 709 30 1106
Fragments arranged by size:
1106 709 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 103 889 1102 1189 1300 1432 1498 1845
Size: 103 786 213 87 111 132 66 347
Fragments arranged by size:
786 347 213 132 111 103 87 66
BseYI C'CCAG_C
Cuts at: 0 78 120 193 1319 1845
Size: 78 42 73 1126 526
Fragments arranged by size:
1126 526 78 73 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 745 1294 1357 1562 1789 1845
Size: 745 549 63 205 227 56
Fragments arranged by size:
745 549 227 205 63 56
BsiEI CG_ry'CG
Cuts at: 0 1183 1409 1845
Size: 1183 226 436
Fragments arranged by size:
1183 436 226
BsiHKAI G_wGCw'C
Cuts at: 0 107 182 1667 1845
Size: 107 75 1485 178
Fragments arranged by size:
1485 178 107 75
Bsp1286I G_dGCh'C
Cuts at: 0 107 182 414 454 578 887 1204 1462
Size: 107 75 232 40 124 309 317 258
Cuts at: 1462 1490 1531 1667 1845
Size: 28 41 136 178
Fragments arranged by size:
317 309 258 232 178 136 124 107
75 41 40 28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 767 1145 1259 1845
Size: 767 378 114 586
Fragments arranged by size:
767 586 378 114
BsrBI CCG'CTC
Cuts at: 0 392 666 1179 1845
Size: 392 274 513 666
Fragments arranged by size:
666 513 392 274
BsrDI GCAATG_nn'
Cuts at: 0 929 1017 1845
Size: 929 88 828
Fragments arranged by size:
929 828 88
BsrFI r'CCGG_y
Cuts at: 0 652 896 1448 1531 1845
Size: 652 244 552 83 314
Fragments arranged by size:
652 552 314 244 83
BstAPI GCAn_nnn'nTGC
Cuts at: 0 801 1377 1845
Size: 801 576 468
Fragments arranged by size:
801 576 468
BstEII G'GTnAC_C
Cuts at: 0 600 1845
Size: 600 1245
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1161 1845
Size: 1161 684
BstYI r'GATC_y
Cuts at: 0 287 867 1091 1845
Size: 287 580 224 754
Fragments arranged by size:
754 580 287 224
Bsu36I CC'TnA_GG
Cuts at: 0 852 1845
Size: 852 993
BtgI C'CryG_G
Cuts at: 0 236 845 1423 1462 1845
Size: 236 609 578 39 383
Fragments arranged by size:
609 578 383 236 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 240 1845
Size: 240 1605
BtsI GCAGTG_nn'
Cuts at: 0 1550 1845
Size: 1550 295
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1314 1349 1845
Size: 1314 35 496
Fragments arranged by size:
1314 496 35
EaeI y'GGCC_r
Cuts at: 0 239 894 898 1046 1180 1406 1533 1712
Size: 239 655 4 148 134 226 127 179
Cuts at: 1712 1845
Size: 133
Fragments arranged by size:
655 239 226 179 148 134 133 127
4
EagI C'GGCC_G
Cuts at: 0 1180 1406 1845
Size: 1180 226 439
Fragments arranged by size:
1180 439 226
EarI CTCTTCn'nnn_
Cuts at: 0 433 1845
Size: 433 1412
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1054 1209 1845
Size: 1054 155 636
Fragments arranged by size:
1054 636 155
EcoICRI GAG'CTC
Cuts at: 0 180 1845
Size: 180 1665
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 321 414 796 901 1162 1183 1210
Size: 28 293 93 382 105 261 21 27
Cuts at: 1210 1622 1845
Size: 412 223
Fragments arranged by size:
412 382 293 261 223 105 93 28
27 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 323 547 1141 1845
Size: 323 224 594 704
Fragments arranged by size:
704 594 323 224
EcoO109I rG'GnC_Cy
Cuts at: 0 503 513 1230 1458 1521 1552 1597 1845
Size: 503 10 717 228 63 31 45 248
Fragments arranged by size:
717 503 248 228 63 45 31 10
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1122 1845
Size: 1122 723
FseI GG_CCGG'CC
Cuts at: 0 900 1845
Size: 900 945
FspI TGC'GCA
Cuts at: 0 1381 1845
Size: 1381 464
FspAI rTGC'GCAy
Cuts at: 0 1381 1845
Size: 1381 464
HaeII r_GCGC'y
Cuts at: 0 299 1736 1845
Size: 299 1437 109
Fragments arranged by size:
1437 299 109
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 284 316 936 968 1845
Size: 284 32 620 32 877
Fragments arranged by size:
877 620 284 32 32
HincII GTy'rAC
Cuts at: 0 150 303 1845
Size: 150 153 1542
Fragments arranged by size:
1542 153 150
KasI G'GCGC_C
Cuts at: 0 295 1845
Size: 295 1550
KpnI G_GTAC'C
Cuts at: 0 843 1845
Size: 843 1002
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 1845
Size: 427 1418
MscI TGG'CCA
Cuts at: 0 1714 1845
Size: 1714 131
MslI CAynn'nnrTG
Cuts at: 0 1303 1845
Size: 1303 542
MspA1I CmG'CkG
Cuts at: 0 36 595 789 1845
Size: 36 559 194 1056
Fragments arranged by size:
1056 559 194 36
NaeI GCC'GGC
Cuts at: 0 898 1450 1845
Size: 898 552 395
Fragments arranged by size:
898 552 395
NarI GG'CG_CC
Cuts at: 0 296 1845
Size: 296 1549
NcoI C'CATG_G
Cuts at: 0 845 1845
Size: 845 1000
NdeI CA'TA_TG
Cuts at: 0 373 1845
Size: 373 1472
NgoMIV G'CCGG_C
Cuts at: 0 896 1448 1845
Size: 896 552 397
Fragments arranged by size:
896 552 397
NspI r_CATG'y
Cuts at: 0 699 981 1845
Size: 699 282 864
Fragments arranged by size:
864 699 282
PasI CC'CwG_GG
Cuts at: 0 516 1845
Size: 516 1329
PciI A'CATG_T
Cuts at: 0 977 1845
Size: 977 868
PflMI CCAn_nnn'nTGG
Cuts at: 0 516 1845
Size: 516 1329
PfoI T'CCnGG_A
Cuts at: 0 524 1058 1226 1845
Size: 524 534 168 619
Fragments arranged by size:
619 534 524 168
PmlI CAC'GTG
Cuts at: 0 1314 1845
Size: 1314 531
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 709 741 1200 1232 1845
Size: 709 32 459 32 613
Fragments arranged by size:
709 613 459 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1230 1552 1845
Size: 1230 322 293
Fragments arranged by size:
1230 322 293
PspOMI G'GGCC_C
Cuts at: 0 410 574 1458 1845
Size: 410 164 884 387
Fragments arranged by size:
884 410 387 164
PspXI vC'TCGA_Gb
Cuts at: 0 1728 1845
Size: 1728 117
PstI C_TGCA'G
Cuts at: 0 788 794 1031 1845
Size: 788 6 237 814
Fragments arranged by size:
814 788 237 6
PvuII CAG'CTG
Cuts at: 0 595 789 1845
Size: 595 194 1056
Fragments arranged by size:
1056 595 194
SacI G_AGCT'C
Cuts at: 0 182 1845
Size: 182 1663
SalI G'TCGA_C
Cuts at: 0 301 1845
Size: 301 1544
SbfI CC_TGCA'GG
Cuts at: 0 1031 1845
Size: 1031 814
SexAI A'CCwGG_T
Cuts at: 0 98 1845
Size: 98 1747
SfcI C'TryA_G
Cuts at: 0 784 790 1027 1845
Size: 784 6 237 818
Fragments arranged by size:
818 784 237 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 511 1405 1845
Size: 511 894 440
Fragments arranged by size:
894 511 440
SfoI GGC'GCC
Cuts at: 0 297 1845
Size: 297 1548
SmaI CCC'GGG
Cuts at: 0 1441 1845
Size: 1441 404
SmlI C'TyrA_G
Cuts at: 0 320 1728 1822 1845
Size: 320 1408 94 23
Fragments arranged by size:
1408 320 94 23
StuI AGG'CCT
Cuts at: 0 1033 1841 1845
Size: 1033 808 4
Fragments arranged by size:
1033 808 4
StyI C'CwwG_G
Cuts at: 0 113 845 1212 1344 1845
Size: 113 732 367 132 501
Fragments arranged by size:
732 501 367 132 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1219 1845
Size: 1219 626
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 216 248 406 438 1222 1254 1845
Size: 216 32 158 32 784 32 591
Fragments arranged by size:
784 591 216 158 32 32 32
XbaI T'CTAG_A
Cuts at: 0 870 1845
Size: 870 975
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 51 345 1845
Size: 51 294 1500
Fragments arranged by size:
1500 294 51
XhoI C'TCGA_G
Cuts at: 0 320 1728 1845
Size: 320 1408 117
Fragments arranged by size:
1408 320 117
XmaI C'CCGG_G
Cuts at: 0 1439 1845
Size: 1439 406
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AloI
AlwNI ApaI AvaI AvrII BanI BanII BbeI BbsI
BbvCI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI
BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI FseI FspI FspAI HaeII Hin4I
HincII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI
PfoI PmlI PpiI PpuMI PspOMI PspXI PstI PvuII
SacI SalI SbfI SexAI SfcI SfiI SfoI SmaI
SmlI StuI StyI TaqII TstI XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI ApaLI ApoI
AscI AseI AsiSI BaeI BamHI BcgI BciVI BclI
BfrBI BmtI BplI BsaBI BsiWI BsmI BsmBI BspEI
BspHI BsrGI BssHII BssSI BstBI BstZ17I ClaI DraI
DraIII DrdI EcoRI EcoRV HindIII HpaI MfeI MluI
NheI NotI NruI NsiI PacI PmeI PshAI PsiI
PsrI PvuI RsrII SacII SanDI SapI ScaI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI
Tth111I XmnI ZraI