Restriction map for
pFN21AB7575
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# NO CUT ENZYMES ( 197 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstBI
BstEII BstPI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
ClaI CpoI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT22I FbaI Fnu4HI
FseI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII ItaI
KasI KpnI KspI MaeI MaeIII
MamI MfeI MflI MluI MluNI
MroI MscI MspA1I MspI MunI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI Sau96I SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TseI Tsp45I Tth111I TthHB8I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 443 GTATAC GT^[AC][GT]AC
AccII 72 CGCG CG^CG
AciI 71 CCGC CCGC(-3/-1)
AfaI 581 GTAC GT^AC
AlwI 172 GATCC GGATC(4/5)
BbvCI 93 CCTCAGC CCTCAGC(-5/-2)
BcgI 82 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 26 CTGGAG CTGGAG(16/14)
BsiYI 217 CCAACAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 217 CCAACAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspHI 374 TCATGA T^CATGA
BsrDI 33 GCAATG GCAATG(2/0)
BssKI 21 CCAGG ^CC[ACGT]GG
Bst1107I 443 GTATAC GTA^TAC
BstAPI 57 GCAACATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 144 GGATG GGATG(2/0)
BstNI 21 CCAGG CC^[AT]GG
BstUI 72 CGCG CG^CG
BstZ17I 443 GTATAC GTA^TAC
DdeI 94 CTCAG C^T[ACGT]AG
DraI 642 TTTAAA TTT^AAA
EarI 268 GAAGAG CTCTTC(1/4)
EcoRII 21 CCAGG ^CC[AT]GG
EcoT14I 99 CCTTGG C^C[AT][AT]GG
FauI 70 CCCGC CCCGC(4/6)
FokI 144 GGATG GGATG(9/13)
HphI 321 TCACC GGTGA(8/7)
Ksp632I 268 GAAGAG CTCTTC(1/4)
MaeII 606 ACGT A^CGT
MslI 280 CACAGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 21 CCAGG CC^[AT]GG
MvnI 72 CGCG CG^CG
PflMI 217 CCAACAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 240 GAGTC GAGTC(4/5)
RcaI 374 TCATGA T^CATGA
RsaI 581 GTAC GT^AC
SapI 268 GAAGAGC GCTCTTC(1/4)
ScrFI 21 CCAGG CC^[ACGT]GG
StyI 99 CCTTGG C^C[AT][AT]GG
Van91I 217 CCAACAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 11 enzymes) #
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AluI 470 AGCT AG^CT
619 AGCT
BsaJI 20 CCCAGG C^C[ACGT][ACGT]GG
99 CCTTGG
BsrI 223 ACTGG ACTGG(1/-1)
326 CCAGT
DpnII 131 GATC ^GATC
172 GATC
EcoRI 370 GAATTC G^AATTC
625 GAATTC
MboI 131 GATC ^GATC
172 GATC
MwoI 10 GCTCCCGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
57 GCAACATCTGC
NdeII 131 GATC ^GATC
172 GATC
NlaIII 375 CATG CATG^
417 CATG
Sau3AI 131 GATC ^GATC
172 GATC
SfaNI 499 GCATC GCATC(5/9)
601 GCATC
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# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
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AcsI ApoI HinfI MboII MnlI
MseI TfiI Tru9I Tsp509I TspRI