(Circular) (Six-base) MAPSORT of: pFN21AB8018.seq Check: 6859 from: 1 to: 6370 pFN21AB8018 6370 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3504 3504 Size: 6370 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4180 278 Size: 53 83 186 936 2644 2468 Fragments arranged by size: 2644 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3713 1937 Size: 1776 4594 Acc65I G'GTAC_C Cuts at: 3691 3691 Size: 6370 AclI AA'CG_TT Cuts at: 1563 4497 4870 1563 Size: 2934 373 3063 Fragments arranged by size: 3063 2934 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3026 4433 5817 6085 1632 Size: 1394 1407 1384 268 1917 Fragments arranged by size: 1917 1407 1394 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2870 820 Size: 197 1853 4320 Fragments arranged by size: 4320 1853 197 AflIII A'CryG_T Cuts at: 1203 2707 5269 6246 1203 Size: 1504 2562 977 1327 Fragments arranged by size: 2562 1504 1327 977 AgeI A'CCGG_T Cuts at: 3450 5201 3450 Size: 1751 4619 AhdI GACnn_n'nnGTC Cuts at: 5099 5099 Size: 6370 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6370 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6370 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3612 3644 3612 Size: 32 6338 AlwNI CAG_nnn'CTG Cuts at: 2125 2146 2606 4113 5685 2125 Size: 21 460 1507 1572 2810 Fragments arranged by size: 2810 1572 1507 460 21 ApaLI G'TGCA_C Cuts at: 2214 4427 5583 2214 Size: 2213 1156 3001 Fragments arranged by size: 3001 2213 1156 ApoI r'AATT_y Cuts at: 1493 2731 3157 3679 3905 4057 6166 1493 Size: 1238 426 522 226 152 2109 1697 Fragments arranged by size: 2109 1697 1238 522 426 226 152 AseI AT'TA_AT Cuts at: 160 1032 4924 160 Size: 872 3892 1606 Fragments arranged by size: 3892 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6370 AvaI C'yCGr_G Cuts at: 1870 1943 3072 3354 3695 1870 Size: 73 1129 282 341 4545 Fragments arranged by size: 4545 1129 341 282 73 BamHI G'GATC_C Cuts at: 3700 4067 3700 Size: 367 6003 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2390 3691 5146 618 Size: 325 217 627 603 1301 1455 1842 Fragments arranged by size: 1842 1455 1301 627 603 325 217 BanII G_rGCy'C Cuts at: 1391 2030 2385 2428 3437 3689 1391 Size: 639 355 43 1009 252 4072 Fragments arranged by size: 4072 1009 639 355 252 43 BbeI G_GCGC'C Cuts at: 2394 2394 Size: 6370 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5399 BbvCI CC'TCA_GC Cuts at: 3595 3595 Size: 6370 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4561 4595 1903 Size: 34 2624 34 3678 Fragments arranged by size: 3678 2624 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2989 4262 5472 2989 Size: 1273 1210 3887 Fragments arranged by size: 3887 1273 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6370 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2399 2408 4981 Size: 107 122 71 631 605 727 9 2573 Cuts at: 4981 136 Size: 1525 Fragments arranged by size: 2573 1525 727 631 605 122 107 71 9 BglII A'GATC_T Cuts at: 2747 3532 6365 2747 Size: 785 2833 2752 Fragments arranged by size: 2833 2752 785 BlpI GC'TnA_GC Cuts at: 3787 3787 Size: 6370 Bme1580I G_kGCm'C Cuts at: 1790 2218 4431 5587 1790 Size: 428 2213 1156 2573 Fragments arranged by size: 2573 2213 1156 428 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6370 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2968 4101 5059 Size: 416 53 83 399 24 1551 1133 958 Cuts at: 5059 442 Size: 1753 Fragments arranged by size: 1753 1551 1133 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6370 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3454 3456 3454 Size: 2 6368 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2168 2651 3442 5030 1239 Size: 929 483 791 1588 2579 Fragments arranged by size: 2579 1588 929 791 483 Bpu10I CC'TnA_GC Cuts at: 3595 3595 Size: 6370 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4495 5360 5658 4495 Size: 865 298 5207 Fragments arranged by size: 5207 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5033 882 Size: 617 3534 2219 Fragments arranged by size: 3534 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2710 4161 493 Size: 711 1506 1451 2702 Fragments arranged by size: 2702 1506 1451 711 BsaBI GATnn'nnATC Cuts at: 6364 6364 Size: 6370 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2391 4177 4559 5194 Size: 53 83 186 936 858 1786 382 635 Cuts at: 5194 275 Size: 1451 Fragments arranged by size: 1786 1451 936 858 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2852 3450 4803 5201 5475 5622 6033 2852 Size: 598 1353 398 274 147 411 3189 Fragments arranged by size: 3189 1353 598 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2174 2204 2893 2923 2941 Size: 30 165 30 771 30 689 30 18 Cuts at: 2941 2971 3440 3470 3612 3642 1178 Size: 30 469 30 142 30 3906 Fragments arranged by size: 3906 771 689 469 165 142 30 30 30 30 30 30 30 18 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2165 3036 3427 3583 1186 Size: 979 871 391 156 3973 Fragments arranged by size: 3973 979 871 391 156 BseYI C'CCAG_C Cuts at: 2155 5573 6069 2155 Size: 3418 496 2456 Fragments arranged by size: 3418 2456 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5797 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3471 3820 4581 5609 1540 Size: 275 186 1470 349 761 1028 2301 Fragments arranged by size: 2301 1470 1028 761 349 275 186 BsiHKAI G_wGCw'C Cuts at: 2218 2385 3437 4431 4516 5587 2218 Size: 167 1052 994 85 1071 3001 Fragments arranged by size: 3001 1071 1052 994 167 85 BsmI GAATG_Cn' Cuts at: 3881 3974 3881 Size: 93 6277 BsmBI CGTCTCn'nnnn_ Cuts at: 3178 3178 Size: 6370 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2218 2385 2428 3437 3689 4431 Size: 399 240 188 167 43 1009 252 742 Cuts at: 4431 4516 5587 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 1009 742 399 252 240 188 167 85 43 BspEI T'CCGG_A Cuts at: 6033 6033 Size: 6370 BspHI T'CATG_A Cuts at: 4259 5989 4259 Size: 1730 4640 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3504 3725 844 Size: 2660 221 3489 Fragments arranged by size: 3489 2660 221 BsrBI CCG'CTC Cuts at: 1394 1823 3409 3475 4257 1394 Size: 429 1586 66 782 3507 Fragments arranged by size: 3507 1586 782 429 66 BsrDI GCAATG_nn' Cuts at: 65 1248 3125 3198 4865 5039 65 Size: 1183 1877 73 1667 174 1396 Fragments arranged by size: 1877 1667 1396 1183 174 73 BsrFI r'CCGG_y Cuts at: 1700 1810 3390 3450 5014 5201 1700 Size: 110 1580 60 1564 187 2869 Fragments arranged by size: 2869 1580 1564 187 110 60 BsrGI T'GTAC_A Cuts at: 96 2679 2944 96 Size: 2583 265 3522 Fragments arranged by size: 3522 2583 265 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6370 BssSI C'ACGA_G Cuts at: 4430 5442 4430 Size: 1012 5358 BstAPI GCAn_nnn'nTGC Cuts at: 794 2125 794 Size: 1331 5039 BstBI TT'CG_AA Cuts at: 5174 5174 Size: 6370 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6370 BstXI CCAn_nnnn'nTGG Cuts at: 2038 2038 Size: 6370 BstYI r'GATC_y Cuts at: 1972 2602 2747 3031 3532 3700 4067 4452 4469 Size: 630 145 284 501 168 367 385 17 Cuts at: 4469 5921 6007 6019 6365 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 630 501 385 367 346 284 168 145 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3138 3138 Size: 6370 BtgI C'CryG_G Cuts at: 513 1065 2004 3290 513 Size: 552 939 1286 3593 Fragments arranged by size: 3593 1286 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5631 Fragments arranged by size: 5631 683 56 BtsI GCAGTG_nn' Cuts at: 3886 4680 4700 3886 Size: 794 20 5556 Fragments arranged by size: 5556 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6370 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2839 2874 3279 3314 529 Size: 35 2275 35 405 35 3585 Fragments arranged by size: 3585 2275 405 35 35 35 DraI TTT'AAA Cuts at: 2565 3674 2565 Size: 1109 5261 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2316 2974 3357 5377 809 Size: 507 1000 658 383 2020 1802 Fragments arranged by size: 2020 1802 1000 658 507 383 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3817 4706 6335 Size: 54 1425 50 275 18 1987 889 1629 Cuts at: 6335 8 Size: 43 Fragments arranged by size: 1987 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3817 1537 Size: 275 2005 4090 Fragments arranged by size: 4090 2005 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3423 4300 1353 Size: 2070 877 3423 Fragments arranged by size: 3423 2070 877 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3039 4957 5331 5477 387 Size: 1064 138 1450 1918 374 146 1280 Fragments arranged by size: 1918 1450 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2383 3435 2383 Size: 1052 5318 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2168 2651 3026 3442 4433 5030 5817 Size: 393 536 483 375 416 991 597 787 Cuts at: 5817 6085 1239 Size: 268 1524 Fragments arranged by size: 1524 991 787 597 536 483 416 393 375 268 EcoNI CCTnn'n_nnAGG Cuts at: 2664 2664 Size: 6370 EcoO109I rG'GnC_Cy Cuts at: 2143 2350 2401 2143 Size: 207 51 6112 Fragments arranged by size: 6112 207 51 EcoRI G'AATT_C Cuts at: 3157 3679 4057 3157 Size: 522 378 5470 Fragments arranged by size: 5470 522 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2340 2533 2340 Size: 193 6177 FspI TGC'GCA Cuts at: 4876 4876 Size: 6370 HaeII r_GCGC'y Cuts at: 1678 2394 2881 3549 5517 1678 Size: 716 487 668 1968 2531 Fragments arranged by size: 2531 1968 716 668 487 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2091 2122 2123 2154 2830 Size: 32 135 32 519 31 1 31 676 Cuts at: 2830 2862 2941 2973 5011 5043 5085 5117 1373 Size: 32 79 32 2038 32 42 32 2626 Fragments arranged by size: 2626 2038 676 519 135 79 42 32 32 32 32 32 32 31 31 1 HincII GTy'rAC Cuts at: 669 1657 1938 3663 3714 3960 669 Size: 988 281 1725 51 246 3079 Fragments arranged by size: 3079 1725 988 281 246 51 HindIII A'AGCT_T Cuts at: 748 748 Size: 6370 HpaI GTT'AAC Cuts at: 3960 3960 Size: 6370 KasI G'GCGC_C Cuts at: 2390 2390 Size: 6370 KpnI G_GTAC'C Cuts at: 3695 3695 Size: 6370 MfeI C'AATT_G Cuts at: 3969 3969 Size: 6370 MluI A'CGCG_T Cuts at: 6246 6246 Size: 6370 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2040 2172 5484 5668 1305 Size: 735 132 3312 184 2007 Fragments arranged by size: 3312 2007 735 184 132 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4548 Fragments arranged by size: 4548 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2766 3257 4328 4687 4846 518 Size: 648 1600 491 1071 359 159 2042 Fragments arranged by size: 2042 1600 1071 648 491 359 159 MspA1I CmG'CkG Cuts at: 1606 2122 2815 3639 3786 4463 5611 5856 1606 Size: 516 693 824 147 677 1148 245 2120 Fragments arranged by size: 2120 1148 824 693 677 516 245 147 NarI GG'CG_CC Cuts at: 2391 2391 Size: 6370 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4879 Fragments arranged by size: 4879 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6370 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6370 NotI GC'GGCC_GC Cuts at: 3817 3817 Size: 6370 NspI r_CATG'y Cuts at: 1132 1229 2628 2680 3728 5273 1132 Size: 97 1399 52 1048 1545 2229 Fragments arranged by size: 2229 1545 1399 1048 97 52 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6370 PciI A'CATG_T Cuts at: 5269 5269 Size: 6370 PflMI CCAn_nnn'nTGG Cuts at: 1108 3131 3281 1108 Size: 2023 150 4197 Fragments arranged by size: 4197 2023 150 PfoI T'CCnGG_A Cuts at: 3027 3561 3027 Size: 534 5836 PmeI GTTT'AAAC Cuts at: 3674 3674 Size: 6370 PmlI CAC'GTG Cuts at: 2710 2710 Size: 6370 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3612 3644 4439 4471 5952 5984 3612 Size: 32 795 32 1481 32 3998 Fragments arranged by size: 3998 1481 795 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2143 2143 Size: 6370 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6370 PsiI TTA'TAA Cuts at: 3940 3940 Size: 6370 PspXI vC'TCGA_Gb Cuts at: 3354 3354 Size: 6370 PstI C_TGCA'G Cuts at: 830 2130 2743 3722 830 Size: 1300 613 979 3478 Fragments arranged by size: 3478 1300 979 613 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6370 PvuII CAG'CTG Cuts at: 2122 2815 3639 2122 Size: 693 824 4853 Fragments arranged by size: 4853 824 693 SacI G_AGCT'C Cuts at: 2385 3437 2385 Size: 1052 5318 SalI G'TCGA_C Cuts at: 1936 3712 1936 Size: 1776 4594 SbfI CC_TGCA'GG Cuts at: 3722 3722 Size: 6370 ScaI AGT'ACT Cuts at: 4618 6330 4618 Size: 1712 4658 SfcI C'TryA_G Cuts at: 826 1045 2126 2499 2739 3718 4853 5534 5725 Size: 219 1081 373 240 979 1135 681 191 Cuts at: 5725 826 Size: 1471 Fragments arranged by size: 1471 1135 1081 979 681 373 240 219 191 SfoI GGC'GCC Cuts at: 2392 2392 Size: 6370 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6370 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6370 SmaI CCC'GGG Cuts at: 1872 3074 3697 1872 Size: 1202 623 4545 Fragments arranged by size: 4545 1202 623 SmlI C'TyrA_G Cuts at: 820 1017 1943 2870 3354 4474 5375 5637 820 Size: 197 926 927 484 1120 901 262 1553 Fragments arranged by size: 1553 1120 927 926 901 484 262 197 SnaBI TAC'GTA Cuts at: 493 4161 493 Size: 3668 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6370 SphI G_CATG'C Cuts at: 1132 3728 1132 Size: 2596 3774 SspI AAT'ATT Cuts at: 5 52 4294 5 Size: 47 4242 2081 Fragments arranged by size: 4242 2081 47 StuI AGG'CCT Cuts at: 1517 2670 1517 Size: 1153 5217 StyI C'CwwG_G Cuts at: 513 1065 2004 2397 3809 513 Size: 552 939 393 1412 3074 Fragments arranged by size: 3074 1412 939 552 393 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3485 4397 4414 4567 4752 5197 325 Size: 1255 1905 912 17 153 185 445 1498 Fragments arranged by size: 1905 1498 1255 912 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2679 2944 4616 Size: 274 80 33 51 1443 702 265 1672 Cuts at: 4616 6328 96 Size: 1712 138 Fragments arranged by size: 1712 1672 1443 702 274 265 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6330 6362 6330 Size: 32 6338 Tth111I GACn'n_nGTC Cuts at: 1316 2774 1316 Size: 1458 4912 XbaI T'CTAG_A Cuts at: 3706 3706 Size: 6370 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2038 2404 2038 Size: 366 6004 XhoI C'TCGA_G Cuts at: 1943 3354 1943 Size: 1411 4959 XmaI C'CCGG_G Cuts at: 1870 3072 3695 1870 Size: 1202 623 4545 Fragments arranged by size: 4545 1202 623 XmnI GAAnn'nnTTC Cuts at: 2778 3161 4499 2778 Size: 383 1338 4649 Fragments arranged by size: 4649 1338 383 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4178 276 Size: 53 83 186 936 2644 2468 Fragments arranged by size: 2644 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI