Restriction map for
pFN21AB6699
(Circular) (Six-base) MAPSORT of: pFN21AB6699.seq Check: 8072 from: 1 to: 5617
pFN21AB6699 5617 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2742 2742
Size: 5617
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3427 278
Size: 53 83 186 936 1891 2468
Fragments arranged by size:
2468 1891 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2960 1937
Size: 1023 4594
Acc65I G'GTAC_C
Cuts at: 2938 2938
Size: 5617
AclI AA'CG_TT
Cuts at: 1563 3744 4117 1563
Size: 2181 373 3063
Fragments arranged by size:
3063 2181 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3680 5064 5332 1632
Size: 2048 1384 268 1917
Fragments arranged by size:
2048 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5420
AflIII A'CryG_T
Cuts at: 1203 2472 4516 5493 1203
Size: 1269 2044 977 1327
Fragments arranged by size:
2044 1327 1269 977
AgeI A'CCGG_T
Cuts at: 2717 4448 2717
Size: 1731 3886
AhdI GACnn_n'nnGTC
Cuts at: 4346 4346
Size: 5617
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5617
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2301 1184
Size: 1117 4500
AlwNI CAG_nnn'CTG
Cuts at: 3360 4932 3360
Size: 1572 4045
ApaI G_GGCC'C
Cuts at: 2414 2529 2570 2414
Size: 115 41 5461
Fragments arranged by size:
5461 115 41
ApaLI G'TGCA_C
Cuts at: 3674 4830 3674
Size: 1156 4461
ApoI r'AATT_y
Cuts at: 1493 2926 3152 3304 5413 1493
Size: 1433 226 152 2109 1697
Fragments arranged by size:
2109 1697 1433 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4171 160
Size: 872 3139 1606
Fragments arranged by size:
3139 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5617
AvaI C'yCGr_G
Cuts at: 1870 1943 2638 2942 1870
Size: 73 695 304 4545
Fragments arranged by size:
4545 695 304 73
BamHI G'GATC_C
Cuts at: 2947 3314 2947
Size: 367 5250
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2938 4393 618
Size: 325 217 627 1151 1455 1842
Fragments arranged by size:
1842 1455 1151 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2414 2465 2529 2570 2936 1391
Size: 1023 51 64 41 366 4072
Fragments arranged by size:
4072 1023 366 64 51 41
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4646
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3808 3842 1903
Size: 34 1871 34 3678
Fragments arranged by size:
3678 1871 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3509 4719 3509
Size: 1210 4407
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5617
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4228 136
Size: 107 122 71 631 605 2556 1525
Fragments arranged by size:
2556 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5612 5612
Size: 5617
BlpI GC'TnA_GC
Cuts at: 3034 3034
Size: 5617
Bme1580I G_kGCm'C
Cuts at: 1790 2414 2529 2570 3678 4834 1790
Size: 624 115 41 1108 1156 2573
Fragments arranged by size:
2573 1156 1108 624 115 41
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5617
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2340 3348 4306
Size: 416 53 83 399 24 923 1008 958
Cuts at: 4306 442
Size: 1753
Fragments arranged by size:
1753 1008 958 923 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5617
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4277 1239
Size: 3038 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3742 4607 4905 3742
Size: 865 298 4454
Fragments arranged by size:
4454 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2248 4280 882
Size: 617 749 2032 2219
Fragments arranged by size:
2219 2032 749 617
BsaAI yAC'GTr
Cuts at: 493 1204 3408 493
Size: 711 2204 2702
Fragments arranged by size:
2702 2204 711
BsaBI GATnn'nnATC
Cuts at: 5611 5611
Size: 5617
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3424 3806 4441 275
Size: 53 83 186 936 1891 382 635 1451
Fragments arranged by size:
1891 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2717 4050 4448 4722 4869 5280 2717
Size: 1333 398 274 147 411 3054
Fragments arranged by size:
3054 1333 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2079 2109 2421 2451 1178
Size: 30 165 30 676 30 312 30 4344
Fragments arranged by size:
4344 676 312 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2774 1186
Size: 1588 4029
BseYI C'CCAG_C
Cuts at: 2403 4820 5316 2403
Size: 2417 496 2704
Fragments arranged by size:
2704 2417 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2168 1163
Size: 573 432 4612
Fragments arranged by size:
4612 573 432
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3067 3828 4856 1540
Size: 275 186 1066 761 1028 2301
Fragments arranged by size:
2301 1066 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2175 2232 2465 2813 3678 3763 4834 2175
Size: 57 233 348 865 85 1071 2958
Fragments arranged by size:
2958 1071 865 348 233 85 57
BsmI GAATG_Cn'
Cuts at: 3128 3221 3128
Size: 93 5524
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2175 2232 2414 2465 2529 2570 2813
Size: 399 385 57 182 51 64 41 243
Cuts at: 2813 2936 3678 3763 4834 1391
Size: 123 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 385 243 182 123
85 64 57 51 41
BspEI T'CCGG_A
Cuts at: 5280 5280
Size: 5617
BspHI T'CATG_A
Cuts at: 3506 5236 3506
Size: 1730 3887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2742 2972 844
Size: 1898 230 3489
Fragments arranged by size:
3489 1898 230
BsrBI CCG'CTC
Cuts at: 1394 1823 3504 1394
Size: 429 1681 3507
Fragments arranged by size:
3507 1681 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2153 4112 4286 65
Size: 1183 905 1959 174 1396
Fragments arranged by size:
1959 1396 1183 905 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2717 4261 4448 1700
Size: 110 907 1544 187 2869
Fragments arranged by size:
2869 1544 907 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5617
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5617
BssSI C'ACGA_G
Cuts at: 3677 4689 3677
Size: 1012 4605
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5617
BstBI TT'CG_AA
Cuts at: 4421 4421
Size: 5617
BstEII G'GTnAC_C
Cuts at: 1184 2720 1184
Size: 1536 4081
BstXI CCAn_nnnn'nTGG
Cuts at: 2422 2422
Size: 5617
BstYI r'GATC_y
Cuts at: 1972 2947 3314 3699 3716 5168 5254 5266 5612
Size: 975 367 385 17 1452 86 12 346
Cuts at: 5612 1972
Size: 1977
Fragments arranged by size:
1977 1452 975 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2415 2578 513
Size: 552 1350 163 3552
Fragments arranged by size:
3552 1350 552 163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2786 487
Size: 683 56 1560 3318
Fragments arranged by size:
3318 1560 683 56
BtsI GCAGTG_nn'
Cuts at: 2648 3133 3927 3947 2648
Size: 485 794 20 4318
Fragments arranged by size:
4318 794 485 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5617
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5582
DraI TTT'AAA
Cuts at: 2921 2921
Size: 5617
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4624 809
Size: 507 3308 1802
Fragments arranged by size:
3308 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2063 3064 3953
Size: 54 1425 50 275 18 233 1001 889
Cuts at: 3953 5582 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1001 889 275 233 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3064 1537
Size: 275 1252 4090
Fragments arranged by size:
4090 1252 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2124 2792 3547 1353
Size: 771 668 755 3423
Fragments arranged by size:
3423 771 755 668
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4204 4578 4724 387
Size: 1064 138 2615 374 146 1280
Fragments arranged by size:
2615 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2463 2463
Size: 5617
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3680 4277 5064 5332 1239
Size: 393 2048 597 787 268 1524
Fragments arranged by size:
2048 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2058 2111 2292 2411 2525 2567 2751 2058
Size: 53 181 119 114 42 184 4924
Fragments arranged by size:
4924 184 181 119 114 53 42
EcoRI G'AATT_C
Cuts at: 2926 3304 2926
Size: 378 5239
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2272 2272
Size: 5617
FspI TGC'GCA
Cuts at: 4123 4123
Size: 5617
HaeII r_GCGC'y
Cuts at: 1678 4764 1678
Size: 3086 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2228 2260 4258 4290 4332
Size: 32 135 32 656 32 1998 32 42
Cuts at: 4332 4364 1373
Size: 32 2626
Fragments arranged by size:
2626 1998 656 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2536 2961 3207 669
Size: 988 281 598 425 246 3079
Fragments arranged by size:
3079 988 598 425 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5617
HpaI GTT'AAC
Cuts at: 3207 3207
Size: 5617
KpnI G_GTAC'C
Cuts at: 2942 2942
Size: 5617
MfeI C'AATT_G
Cuts at: 3216 3216
Size: 5617
MluI A'CGCG_T
Cuts at: 5493 5493
Size: 5617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2838 4731 4915 1305
Size: 1533 1893 184 2007
Fragments arranged by size:
2007 1893 1533 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2065 10
Size: 54 1425 343 233 3562
Fragments arranged by size:
3562 1425 343 233 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2235 2736 3575 3934 4093 518
Size: 648 1069 501 839 359 159 2042
Fragments arranged by size:
2042 1069 839 648 501 359 159
MspA1I CmG'CkG
Cuts at: 1606 3033 3710 4858 5103 1606
Size: 1427 677 1148 245 2120
Fragments arranged by size:
2120 1427 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5065
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5617
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5617
NotI GC'GGCC_GC
Cuts at: 3064 3064
Size: 5617
NspI r_CATG'y
Cuts at: 1132 1229 2476 2741 2975 4520 1132
Size: 97 1247 265 234 1545 2229
Fragments arranged by size:
2229 1545 1247 265 234 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5617
PciI A'CATG_T
Cuts at: 2472 4516 2472
Size: 2044 3573
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5617
PfoI T'CCnGG_A
Cuts at: 2456 2456
Size: 5617
PmeI GTTT'AAAC
Cuts at: 2921 2921
Size: 5617
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3686 3718 5199 5231 3686
Size: 32 1481 32 4072
Fragments arranged by size:
4072 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2111 2292 2751 2111
Size: 181 459 4977
Fragments arranged by size:
4977 459 181
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5617
PsiI TTA'TAA
Cuts at: 3187 3187
Size: 5617
PspOMI G'GGCC_C
Cuts at: 2410 2525 2566 2410
Size: 115 41 5461
Fragments arranged by size:
5461 115 41
PspXI vC'TCGA_Gb
Cuts at: 2638 2638
Size: 5617
PstI C_TGCA'G
Cuts at: 830 2055 2453 2524 2854 2969 830
Size: 1225 398 71 330 115 3478
Fragments arranged by size:
3478 1225 398 330 115 71
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5617
SacI G_AGCT'C
Cuts at: 2465 2465
Size: 5617
SalI G'TCGA_C
Cuts at: 1936 2959 1936
Size: 1023 4594
SbfI CC_TGCA'GG
Cuts at: 2854 2969 2854
Size: 115 5502
ScaI AGT'ACT
Cuts at: 3865 5577 3865
Size: 1712 3905
SfcI C'TryA_G
Cuts at: 826 1045 2051 2296 2449 2520 2590 2850 2965
Size: 219 1006 245 153 71 70 260 115
Cuts at: 2965 4100 4781 4972 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1006 681 260 245 219 191
153 115 71 70
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5617
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5617
SmaI CCC'GGG
Cuts at: 1872 2944 1872
Size: 1072 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2638 3721 4622 4884 820
Size: 197 926 695 1083 901 262 1553
Fragments arranged by size:
1553 1083 926 901 695 262 197
SnaBI TAC'GTA
Cuts at: 493 3408 493
Size: 2915 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5617
SphI G_CATG'C
Cuts at: 1132 2975 1132
Size: 1843 3774
SspI AAT'ATT
Cuts at: 5 52 3541 5
Size: 47 3489 2081
Fragments arranged by size:
3489 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5617
StyI C'CwwG_G
Cuts at: 513 1065 2247 2529 3056 513
Size: 552 1182 282 527 3074
Fragments arranged by size:
3074 1182 552 527 282
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2121 2255 2591 2710 3644 3661 3814
Size: 1255 541 134 336 119 934 17 153
Cuts at: 3814 3999 4444 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 934 541 445 336 185 153
134 119 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3863 5575 96
Size: 274 80 33 51 1443 1886 1712 138
Fragments arranged by size:
1886 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2421 2453 5577 5609 2421
Size: 32 3124 32 2429
Fragments arranged by size:
3124 2429 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2363 2443 1316
Size: 1047 80 4490
Fragments arranged by size:
4490 1047 80
XbaI T'CTAG_A
Cuts at: 2953 2953
Size: 5617
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2353 2011
Size: 342 5275
XhoI C'TCGA_G
Cuts at: 1943 2638 1943
Size: 695 4922
XmaI C'CCGG_G
Cuts at: 1870 2942 1870
Size: 1072 4545
XmnI GAAnn'nnTTC
Cuts at: 2089 2674 3746 2089
Size: 585 1072 3960
Fragments arranged by size:
3960 1072 585
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3425 276
Size: 53 83 186 936 1891 2468
Fragments arranged by size:
2468 1891 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI