(Circular) (Six-base) MAPSORT of: pFN21AB6698.seq Check: 4562 from: 1 to: 6040 pFN21AB6698 6040 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2726 2726 Size: 6040 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3850 278 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2343 3383 1937 Size: 406 1040 4594 Fragments arranged by size: 4594 1040 406 Acc65I G'GTAC_C Cuts at: 3361 3361 Size: 6040 AclI AA'CG_TT Cuts at: 1563 4167 4540 1563 Size: 2604 373 3063 Fragments arranged by size: 3063 2604 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2242 2488 2669 3107 4103 5487 5755 1632 Size: 610 246 181 438 996 1384 268 1917 Fragments arranged by size: 1917 1384 996 610 438 268 246 181 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5843 AflIII A'CryG_T Cuts at: 1203 2148 4939 5916 1203 Size: 945 2791 977 1327 Fragments arranged by size: 2791 1327 977 945 AgeI A'CCGG_T Cuts at: 4871 4871 Size: 6040 AhdI GACnn_n'nnGTC Cuts at: 3259 4769 3259 Size: 1510 4530 AleI CACnn'nnGTG Cuts at: 1166 2540 1166 Size: 1374 4666 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6040 AlwNI CAG_nnn'CTG Cuts at: 3133 3783 5355 3133 Size: 650 1572 3818 Fragments arranged by size: 3818 1572 650 ApaLI G'TGCA_C Cuts at: 2018 4097 5253 2018 Size: 2079 1156 2805 Fragments arranged by size: 2805 2079 1156 ApoI r'AATT_y Cuts at: 1493 3349 3575 3727 5836 1493 Size: 1856 226 152 2109 1697 Fragments arranged by size: 2109 1856 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4594 160 Size: 872 3562 1606 Fragments arranged by size: 3562 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 AvaI C'yCGr_G Cuts at: 1870 1943 2187 2731 2864 3365 1870 Size: 73 244 544 133 501 4545 Fragments arranged by size: 4545 544 501 244 133 73 BamHI G'GATC_C Cuts at: 3036 3370 3737 3036 Size: 334 367 5339 Fragments arranged by size: 5339 367 334 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2054 2216 2447 2776 2860 Size: 325 217 627 267 162 231 329 84 Cuts at: 2860 3361 4816 618 Size: 501 1455 1842 Fragments arranged by size: 1842 1455 627 501 329 325 267 231 217 162 84 BanII G_rGCy'C Cuts at: 1391 2550 2871 3359 1391 Size: 1159 321 488 4072 Fragments arranged by size: 4072 1159 488 321 BbeI G_GCGC'C Cuts at: 2058 2058 Size: 6040 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3059 928 Size: 971 1160 3909 Fragments arranged by size: 3909 1160 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2032 2066 3007 3041 4231 Size: 34 50 34 11 34 941 34 1190 Cuts at: 4231 4265 1903 Size: 34 3678 Fragments arranged by size: 3678 1190 941 50 34 34 34 34 34 11 BciVI GTATCCnnnnn_n' Cuts at: 3932 5142 3932 Size: 1210 4830 BclI T'GATC_A Cuts at: 1548 1548 Size: 6040 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2040 4651 136 Size: 107 122 71 631 605 368 2611 1525 Fragments arranged by size: 2611 1525 631 605 368 122 107 71 BglII A'GATC_T Cuts at: 2253 6035 2253 Size: 3782 2258 BlpI GC'TnA_GC Cuts at: 3457 3457 Size: 6040 Bme1580I G_kGCm'C Cuts at: 1790 2022 2450 2781 2865 4101 5257 1790 Size: 232 428 331 84 1236 1156 2573 Fragments arranged by size: 2573 1236 1156 428 331 232 84 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6040 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2314 3771 4729 Size: 416 53 83 399 24 897 1457 958 Cuts at: 4729 442 Size: 1753 Fragments arranged by size: 1753 1457 958 897 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6040 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2228 2378 4700 1239 Size: 917 72 150 2322 2579 Fragments arranged by size: 2579 2322 917 150 72 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4165 5030 5328 4165 Size: 865 298 4877 Fragments arranged by size: 4877 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4703 882 Size: 617 3204 2219 Fragments arranged by size: 3204 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3831 493 Size: 711 2627 2702 Fragments arranged by size: 2702 2627 711 BsaBI GATnn'nnATC Cuts at: 6034 6034 Size: 6040 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2055 3285 3847 4229 Size: 53 83 186 936 522 1230 562 382 Cuts at: 4229 4864 275 Size: 635 1451 Fragments arranged by size: 1451 1230 936 635 562 522 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4473 4871 5145 5292 5703 4473 Size: 398 274 147 411 4810 Fragments arranged by size: 4810 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5815 Fragments arranged by size: 5815 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3329 3332 3335 1186 Size: 2143 3 3 3891 Fragments arranged by size: 3891 2143 3 3 BseYI C'CCAG_C Cuts at: 2874 5243 5739 2874 Size: 2369 496 3175 Fragments arranged by size: 3175 2369 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2777 3023 3173 1163 Size: 573 1041 246 150 4030 Fragments arranged by size: 4030 1041 573 246 150 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2929 3060 3294 3490 4251 5279 Size: 275 186 928 131 234 196 761 1028 Cuts at: 5279 1540 Size: 2301 Fragments arranged by size: 2301 1028 928 761 275 234 196 186 131 BsiHKAI G_wGCw'C Cuts at: 2022 2871 4101 4186 5257 2022 Size: 849 1230 85 1071 2805 Fragments arranged by size: 2805 1230 1071 849 85 BsmI GAATG_Cn' Cuts at: 3551 3644 3551 Size: 93 5947 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2450 2550 2781 2865 2871 3359 Size: 399 232 428 100 231 84 6 488 Cuts at: 3359 4101 4186 5257 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 488 428 399 232 231 100 85 84 6 BspEI T'CCGG_A Cuts at: 5703 5703 Size: 6040 BspHI T'CATG_A Cuts at: 2493 3929 5659 2493 Size: 1436 1730 2874 Fragments arranged by size: 2874 1730 1436 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2726 2760 3395 844 Size: 1882 34 635 3489 Fragments arranged by size: 3489 1882 635 34 BsrBI CCG'CTC Cuts at: 1394 1823 2706 3927 1394 Size: 429 883 1221 3507 Fragments arranged by size: 3507 1221 883 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4535 4709 65 Size: 1183 3287 174 1396 Fragments arranged by size: 3287 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4684 4871 1700 Size: 110 2874 187 2869 Fragments arranged by size: 2874 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6040 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6040 BssSI C'ACGA_G Cuts at: 4100 5112 4100 Size: 1012 5028 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6040 BstBI TT'CG_AA Cuts at: 4844 4844 Size: 6040 BstEII G'GTnAC_C Cuts at: 1184 2710 1184 Size: 1526 4514 BstXI CCAn_nnnn'nTGG Cuts at: 2200 2200 Size: 6040 BstYI r'GATC_y Cuts at: 1972 2203 2253 2466 3036 3370 3737 4122 4139 Size: 231 50 213 570 334 367 385 17 Cuts at: 4139 5591 5677 5689 6035 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 570 385 367 346 334 231 213 86 50 17 12 BtgI C'CryG_G Cuts at: 513 1065 2113 2220 2923 3079 3288 513 Size: 552 1048 107 703 156 209 3265 Fragments arranged by size: 3265 1048 703 552 209 156 107 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2094 487 Size: 683 56 868 4433 Fragments arranged by size: 4433 868 683 56 BtsI GCAGTG_nn' Cuts at: 3556 4350 4370 3556 Size: 794 20 5226 Fragments arranged by size: 5226 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6040 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6005 DraI TTT'AAA Cuts at: 3344 3344 Size: 6040 DraIII CAC_nnn'GTG Cuts at: 2689 2689 Size: 6040 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5047 809 Size: 507 3731 1802 Fragments arranged by size: 3731 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2248 2281 2694 Size: 54 1425 50 275 18 418 33 413 Cuts at: 2694 2904 2926 3057 3096 3120 3162 3291 3487 Size: 210 22 131 39 24 42 129 196 Cuts at: 3487 4376 6005 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 418 413 275 210 196 131 129 54 50 43 42 39 33 24 22 18 EagI C'GGCC_G Cuts at: 1537 1812 2926 3057 3291 3487 1537 Size: 275 1114 131 234 196 4090 Fragments arranged by size: 4090 1114 275 234 196 131 EarI CTCTTCn'nnn_ Cuts at: 1353 2469 3970 1353 Size: 1116 1501 3423 Fragments arranged by size: 3423 1501 1116 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4627 5001 5147 387 Size: 1064 138 3038 374 146 1280 Fragments arranged by size: 3038 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2869 2869 Size: 6040 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2228 2242 2378 2488 2669 3107 Size: 393 524 72 14 136 110 181 438 Cuts at: 3107 4103 4700 5487 5755 1239 Size: 996 597 787 268 1524 Fragments arranged by size: 1524 996 787 597 524 438 393 268 181 136 110 72 14 EcoO109I rG'GnC_Cy Cuts at: 2620 2674 2620 Size: 54 5986 EcoRI G'AATT_C Cuts at: 3349 3727 3349 Size: 378 5662 FspI TGC'GCA Cuts at: 2756 4546 2756 Size: 1790 4250 HaeII r_GCGC'y Cuts at: 1678 2058 2855 5187 1678 Size: 380 797 2332 2531 Fragments arranged by size: 2531 2332 797 380 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2401 2433 3245 3277 4681 Size: 32 135 32 829 32 812 32 1404 Cuts at: 4681 4713 4755 4787 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1404 829 812 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2344 3384 3630 669 Size: 988 281 406 1040 246 3079 Fragments arranged by size: 3079 1040 988 406 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6040 HpaI GTT'AAC Cuts at: 3630 3630 Size: 6040 KasI G'GCGC_C Cuts at: 2054 2054 Size: 6040 KpnI G_GTAC'C Cuts at: 3365 3365 Size: 6040 MfeI C'AATT_G Cuts at: 3639 3639 Size: 6040 MluI A'CGCG_T Cuts at: 5916 5916 Size: 6040 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5154 5338 1305 Size: 3849 184 2007 Fragments arranged by size: 3849 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4218 Fragments arranged by size: 4218 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2198 2540 2969 3164 3998 4357 4516 Size: 648 1032 342 429 195 834 359 159 Cuts at: 4516 518 Size: 2042 Fragments arranged by size: 2042 1032 834 648 429 359 342 195 159 MspA1I CmG'CkG Cuts at: 1606 2134 2407 2578 2740 3081 3456 4133 5281 Size: 528 273 171 162 341 375 677 1148 Cuts at: 5281 5526 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 528 375 341 273 245 171 162 NarI GG'CG_CC Cuts at: 2055 2055 Size: 6040 NcoI C'CATG_G Cuts at: 513 1065 2113 2220 513 Size: 552 1048 107 4333 Fragments arranged by size: 4333 1048 552 107 NdeI CA'TA_TG Cuts at: 387 387 Size: 6040 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6040 NotI GC'GGCC_GC Cuts at: 3487 3487 Size: 6040 NspI r_CATG'y Cuts at: 1132 1229 2968 3398 4943 1132 Size: 97 1739 430 1545 2229 Fragments arranged by size: 2229 1739 1545 430 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6040 PciI A'CATG_T Cuts at: 4939 4939 Size: 6040 PflMI CCAn_nnn'nTGG Cuts at: 1108 2064 2419 2578 2581 1108 Size: 956 355 159 3 4567 Fragments arranged by size: 4567 956 355 159 3 PfoI T'CCnGG_A Cuts at: 2352 2438 2469 3126 2352 Size: 86 31 657 5266 Fragments arranged by size: 5266 657 86 31 PmeI GTTT'AAAC Cuts at: 3344 3344 Size: 6040 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3209 3241 4109 4141 5622 5654 3209 Size: 32 868 32 1481 32 3595 Fragments arranged by size: 3595 1481 868 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2674 2674 Size: 6040 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6040 PsiI TTA'TAA Cuts at: 3610 3610 Size: 6040 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3005 3037 3005 Size: 32 6008 PstI C_TGCA'G Cuts at: 830 3392 830 Size: 2562 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6040 PvuII CAG'CTG Cuts at: 2134 2407 2578 2740 2134 Size: 273 171 162 5434 Fragments arranged by size: 5434 273 171 162 SacI G_AGCT'C Cuts at: 2871 2871 Size: 6040 SacII CC_GC'GG Cuts at: 3082 3082 Size: 6040 SalI G'TCGA_C Cuts at: 1936 2342 3382 1936 Size: 406 1040 4594 Fragments arranged by size: 4594 1040 406 SbfI CC_TGCA'GG Cuts at: 3392 3392 Size: 6040 ScaI AGT'ACT Cuts at: 4288 6000 4288 Size: 1712 4328 SexAI A'CCwGG_T Cuts at: 2685 3255 2685 Size: 570 5470 SfcI C'TryA_G Cuts at: 826 1045 3388 4523 5204 5395 826 Size: 219 2343 1135 681 191 1471 Fragments arranged by size: 2343 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2056 2056 Size: 6040 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6040 SmaI CCC'GGG Cuts at: 1872 2733 3367 1872 Size: 861 634 4545 Fragments arranged by size: 4545 861 634 SmlI C'TyrA_G Cuts at: 820 1017 1943 4144 5045 5307 820 Size: 197 926 2201 901 262 1553 Fragments arranged by size: 2201 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3831 493 Size: 3338 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6040 SphI G_CATG'C Cuts at: 1132 3398 1132 Size: 2266 3774 SspI AAT'ATT Cuts at: 5 52 3964 5 Size: 47 3912 2081 Fragments arranged by size: 3912 2081 47 StuI AGG'CCT Cuts at: 1517 3182 1517 Size: 1665 4375 StyI C'CwwG_G Cuts at: 513 1065 2113 2220 2310 2523 3479 513 Size: 552 1048 107 90 213 956 3074 Fragments arranged by size: 3074 1048 956 552 213 107 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2338 2536 2557 2888 4067 4084 4237 Size: 1255 758 198 21 331 1179 17 153 Cuts at: 4237 4422 4867 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1179 758 445 331 198 185 153 21 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2151 4286 5998 Size: 274 80 33 51 1443 174 2135 1712 Cuts at: 5998 96 Size: 138 Fragments arranged by size: 2135 1712 1443 274 174 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6000 6032 6000 Size: 32 6008 Tth111I GACn'n_nGTC Cuts at: 1316 2228 2348 1316 Size: 912 120 5008 Fragments arranged by size: 5008 912 120 XbaI T'CTAG_A Cuts at: 3376 3376 Size: 6040 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2899 2977 2899 Size: 78 5962 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6040 XmaI C'CCGG_G Cuts at: 1870 2731 3365 1870 Size: 861 634 4545 Fragments arranged by size: 4545 861 634 XmnI GAAnn'nnTTC Cuts at: 2501 4169 2501 Size: 1668 4372 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3848 276 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SanDI SapI SfiI SrfI SwaI