(Circular) (Six-base) MAPSORT of: pFN21AB0015.seq Check: 3988 from: 1 to: 9478 pFN21AB0015 9478 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5164 5617 5164 Size: 453 9025 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3720 4119 7288 278 Size: 53 83 186 936 2184 399 3169 2468 Fragments arranged by size: 3169 2468 2184 936 399 186 83 53 AccI GT'mk_AC Cuts at: 1937 4232 6821 1937 Size: 2295 2589 4594 Fragments arranged by size: 4594 2589 2295 Acc65I G'GTAC_C Cuts at: 6799 6799 Size: 9478 AclI AA'CG_TT Cuts at: 1563 7605 7978 1563 Size: 6042 373 3063 Fragments arranged by size: 6042 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2270 4323 4649 4696 4871 5636 5891 5903 Size: 638 2053 326 47 175 765 255 12 Cuts at: 5903 6143 7541 8925 9193 1632 Size: 240 1398 1384 268 1917 Fragments arranged by size: 2053 1917 1398 1384 765 638 326 268 255 240 175 47 12 AflII C'TTAA_G Cuts at: 820 1017 3356 820 Size: 197 2339 6942 Fragments arranged by size: 6942 2339 197 AflIII A'CryG_T Cuts at: 1203 3381 4235 4440 8377 9354 1203 Size: 2178 854 205 3937 977 1327 Fragments arranged by size: 3937 2178 1327 977 854 205 AgeI A'CCGG_T Cuts at: 8309 8309 Size: 9478 AhdI GACnn_n'nnGTC Cuts at: 2259 4024 5470 8207 2259 Size: 1765 1446 2737 3530 Fragments arranged by size: 3530 2737 1765 1446 AleI CACnn'nnGTG Cuts at: 1166 2072 2225 6417 1166 Size: 906 153 4192 4227 Fragments arranged by size: 4227 4192 906 153 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2466 3612 4010 5052 1184 Size: 1282 1146 398 1042 5610 Fragments arranged by size: 5610 1282 1146 1042 398 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3938 3970 3938 Size: 32 9446 AlwNI CAG_nnn'CTG Cuts at: 2680 3568 5542 7221 8793 2680 Size: 888 1974 1679 1572 3365 Fragments arranged by size: 3365 1974 1679 1572 888 ApaI G_GGCC'C Cuts at: 4884 5266 6469 6519 4884 Size: 382 1203 50 7843 Fragments arranged by size: 7843 1203 382 50 ApaLI G'TGCA_C Cuts at: 2642 3424 7535 8691 2642 Size: 782 4111 1156 3429 Fragments arranged by size: 4111 3429 1156 782 ApoI r'AATT_y Cuts at: 1493 2039 2060 2364 4367 4954 5249 6731 6787 Size: 546 21 304 2003 587 295 1482 56 Cuts at: 6787 7013 7165 9274 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 2003 1697 1482 587 546 304 295 226 152 56 21 AseI AT'TA_AT Cuts at: 160 1032 8032 160 Size: 872 7000 1606 Fragments arranged by size: 7000 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9478 AvaI C'yCGr_G Cuts at: 1870 1943 2700 4308 6518 6564 6726 6803 1870 Size: 73 757 1608 2210 46 162 77 4545 Fragments arranged by size: 4545 2210 1608 757 162 77 73 46 AvrII C'CTAG_G Cuts at: 3064 3064 Size: 9478 BamHI G'GATC_C Cuts at: 3132 3860 6808 7175 3132 Size: 728 2948 367 5435 Fragments arranged by size: 5435 2948 728 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3785 5176 5957 6639 6799 Size: 325 217 627 1998 1391 781 682 160 Cuts at: 6799 8254 618 Size: 1455 1842 Fragments arranged by size: 1998 1842 1455 1391 781 682 627 325 217 160 BanII G_rGCy'C Cuts at: 1391 3234 4884 5266 5658 5892 6149 6469 6519 Size: 1843 1650 382 392 234 257 320 50 Cuts at: 6519 6797 1391 Size: 278 4072 Fragments arranged by size: 4072 1843 1650 392 382 320 278 257 234 50 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2258 2544 3712 5332 5867 928 Size: 971 359 286 1168 1620 535 4539 Fragments arranged by size: 4539 1620 1168 971 535 359 286 BbvCI CC'TCA_GC Cuts at: 3624 5773 3624 Size: 2149 7329 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7669 7703 1903 Size: 34 5732 34 3678 Fragments arranged by size: 5732 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5251 7370 8580 5251 Size: 2119 1210 6149 Fragments arranged by size: 6149 2119 1210 BclI T'GATC_A Cuts at: 1548 2125 1548 Size: 577 8901 BfrBI ATG'CAT Cuts at: 3098 5057 5753 3098 Size: 1959 696 6823 Fragments arranged by size: 6823 1959 696 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3205 5777 6648 Size: 107 122 71 631 605 1533 2572 871 Cuts at: 6648 8089 136 Size: 1441 1525 Fragments arranged by size: 2572 1533 1525 1441 871 631 605 122 107 71 BglII A'GATC_T Cuts at: 2331 3037 3696 6151 9473 2331 Size: 706 659 2455 3322 2336 Fragments arranged by size: 3322 2455 2336 706 659 BlpI GC'TnA_GC Cuts at: 5657 5990 6895 5657 Size: 333 905 8240 Fragments arranged by size: 8240 905 333 Bme1580I G_kGCm'C Cuts at: 1790 2646 3428 3832 4884 5266 5744 5960 6469 Size: 856 782 404 1052 382 478 216 509 Cuts at: 6469 6519 6644 7539 8695 1790 Size: 50 125 895 1156 2573 Fragments arranged by size: 2573 1156 1052 895 856 782 509 478 404 382 216 125 50 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9478 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2518 6318 6472 Size: 416 53 83 399 24 1101 3800 154 Cuts at: 6472 7209 8167 442 Size: 737 958 1753 Fragments arranged by size: 3800 1753 1101 958 737 416 399 154 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9478 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2893 2893 Size: 9478 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3143 4851 5150 5576 5742 6158 8138 1239 Size: 1904 1708 299 426 166 416 1980 2579 Fragments arranged by size: 2579 1980 1904 1708 426 416 299 166 Bpu10I CC'TnA_GC Cuts at: 3624 5773 5781 3624 Size: 2149 8 7321 Fragments arranged by size: 7321 2149 8 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5993 6338 7603 8468 8766 5993 Size: 345 1265 865 298 6705 Fragments arranged by size: 6705 1265 865 345 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2579 8141 882 Size: 617 1080 5562 2219 Fragments arranged by size: 5562 2219 1080 617 BsaAI yAC'GTr Cuts at: 493 1204 3382 3877 4225 5146 7269 493 Size: 711 2178 495 348 921 2123 2702 Fragments arranged by size: 2702 2178 2123 921 711 495 348 BsaBI GATnn'nnATC Cuts at: 9472 9472 Size: 9478 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3717 4116 5763 7285 Size: 53 83 186 936 2184 399 1647 1522 Cuts at: 7285 7667 8302 275 Size: 382 635 1451 Fragments arranged by size: 2184 1647 1522 1451 936 635 399 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3630 5373 6690 7911 8309 8583 8730 9141 3630 Size: 1743 1317 1221 398 274 147 411 3967 Fragments arranged by size: 3967 1743 1317 1221 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3013 3043 3938 3968 5549 Size: 30 165 30 1610 30 895 30 1581 Cuts at: 5549 5579 1178 Size: 30 5077 Fragments arranged by size: 5077 1610 1581 895 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2346 2423 2782 3104 3423 3962 3967 4217 Size: 1160 77 359 322 319 539 5 250 Cuts at: 4217 4302 4326 5078 5434 6524 6721 6782 1186 Size: 85 24 752 356 1090 197 61 3882 Fragments arranged by size: 3882 1160 1090 752 539 359 356 322 319 250 197 85 77 61 24 5 BseYI C'CCAG_C Cuts at: 2625 4646 4883 5188 5396 5485 5961 8681 9177 Size: 2021 237 305 208 89 476 2720 496 Cuts at: 9177 2625 Size: 2926 Fragments arranged by size: 2926 2720 2021 496 476 305 237 208 89 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3767 4247 5096 5555 6081 1163 Size: 573 2031 480 849 459 526 4560 Fragments arranged by size: 4560 2031 849 573 526 480 459 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6928 7689 8717 1540 Size: 275 186 4927 761 1028 2301 Fragments arranged by size: 4927 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2105 2646 3127 3428 4472 5658 5892 6318 7539 Size: 541 481 301 1044 1186 234 426 1221 Cuts at: 7539 7624 8695 2105 Size: 85 1071 2888 Fragments arranged by size: 2888 1221 1186 1071 1044 541 481 426 301 234 85 BsmI GAATG_Cn' Cuts at: 3100 4887 5390 5755 6989 7082 3100 Size: 1787 503 365 1234 93 5496 Fragments arranged by size: 5496 1787 1234 503 365 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2303 2864 4162 4308 2303 Size: 561 1298 146 7473 Fragments arranged by size: 7473 1298 561 146 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2105 2646 3127 3234 3428 3832 4472 Size: 399 315 541 481 107 194 404 640 Cuts at: 4472 4884 5266 5658 5744 5892 5960 6149 6318 Size: 412 382 392 86 148 68 189 169 Cuts at: 6318 6469 6519 6644 6797 7539 7624 8695 1391 Size: 151 50 125 153 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 640 541 481 412 404 399 392 382 315 194 189 169 153 151 148 125 107 86 85 68 50 BspEI T'CCGG_A Cuts at: 5373 6690 9141 5373 Size: 1317 2451 5710 Fragments arranged by size: 5710 2451 1317 BspHI T'CATG_A Cuts at: 7367 9097 7367 Size: 1730 7748 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2918 5118 5164 5617 6833 844 Size: 2074 2200 46 453 1216 3489 Fragments arranged by size: 3489 2200 2074 1216 453 46 BsrBI CCG'CTC Cuts at: 1394 1823 2752 7365 1394 Size: 429 929 4613 3507 Fragments arranged by size: 4613 3507 929 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2504 3342 3504 6121 6718 7973 8147 Size: 1183 1256 838 162 2617 597 1255 174 Cuts at: 8147 65 Size: 1396 Fragments arranged by size: 2617 1396 1256 1255 1183 838 597 174 162 BsrFI r'CCGG_y Cuts at: 1700 1810 2659 8122 8309 1700 Size: 110 849 5463 187 2869 Fragments arranged by size: 5463 2869 849 187 110 BsrGI T'GTAC_A Cuts at: 96 6183 96 Size: 6087 3391 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9478 BssSI C'ACGA_G Cuts at: 7538 8550 7538 Size: 1012 8466 BstAPI GCAn_nnn'nTGC Cuts at: 794 3520 3525 5209 794 Size: 2726 5 1684 5063 Fragments arranged by size: 5063 2726 1684 5 BstBI TT'CG_AA Cuts at: 8282 8282 Size: 9478 BstEII G'GTnAC_C Cuts at: 1184 3185 1184 Size: 2001 7477 BstXI CCAn_nnnn'nTGG Cuts at: 3820 4759 6245 3820 Size: 939 1486 7053 Fragments arranged by size: 7053 1486 939 BstYI r'GATC_y Cuts at: 1972 2331 3037 3132 3565 3672 3696 3860 5152 Size: 359 706 95 433 107 24 164 1292 Cuts at: 5152 6151 6657 6808 7175 7560 7577 9029 9115 Size: 999 506 151 367 385 17 1452 86 Cuts at: 9115 9127 9473 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1292 999 706 506 433 385 367 359 346 164 151 107 95 86 24 17 12 BstZ17I GTA'TAC Cuts at: 4233 4233 Size: 9478 Bsu36I CC'TnA_GG Cuts at: 3087 4986 3087 Size: 1899 7579 BtgI C'CryG_G Cuts at: 513 1065 2223 5574 6762 513 Size: 552 1158 3351 1188 3229 Fragments arranged by size: 3351 3229 1188 1158 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8739 Fragments arranged by size: 8739 683 56 BtsI GCAGTG_nn' Cuts at: 2009 2511 2587 3275 4288 4467 5973 6062 6994 Size: 502 76 688 1013 179 1506 89 932 Cuts at: 6994 7788 7808 2009 Size: 794 20 3679 Fragments arranged by size: 3679 1506 1013 932 794 688 502 179 89 76 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9478 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9443 DraI TTT'AAA Cuts at: 6782 6782 Size: 9478 DraIII CAC_nnn'GTG Cuts at: 5853 5853 Size: 9478 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8485 809 Size: 507 7169 1802 Fragments arranged by size: 7169 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2920 3456 3651 Size: 54 1425 50 275 18 1090 536 195 Cuts at: 3651 4072 4479 5715 6925 7814 9443 8 Size: 421 407 1236 1210 889 1629 43 Fragments arranged by size: 1629 1425 1236 1210 1090 889 536 421 407 275 195 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6925 1537 Size: 275 5113 4090 Fragments arranged by size: 5113 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2093 3360 4130 6137 6422 7408 1353 Size: 740 1267 770 2007 285 986 3423 Fragments arranged by size: 3423 2007 1267 986 770 740 285 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8065 8439 8585 387 Size: 1064 138 6476 374 146 1280 Fragments arranged by size: 6476 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5656 5890 5656 Size: 234 9244 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2270 3143 4323 4649 4696 4851 4871 Size: 393 638 873 1180 326 47 155 20 Cuts at: 4871 5150 5576 5636 5742 5891 5903 6143 6158 Size: 279 426 60 106 149 12 240 15 Cuts at: 6158 7541 8138 8925 9193 1239 Size: 1383 597 787 268 1524 Fragments arranged by size: 1524 1383 1180 873 787 638 597 426 393 326 279 268 240 155 149 106 60 47 20 15 12 EcoNI CCTnn'n_nnAGG Cuts at: 5729 5729 Size: 9478 EcoO109I rG'GnC_Cy Cuts at: 2279 3068 3835 4880 5262 5263 5769 6466 6515 Size: 789 767 1045 382 1 506 697 49 Cuts at: 6515 2279 Size: 5242 Fragments arranged by size: 5242 1045 789 767 697 506 382 49 1 EcoRI G'AATT_C Cuts at: 4954 5249 6787 7165 4954 Size: 295 1538 378 7267 Fragments arranged by size: 7267 1538 378 295 EcoRV GAT'ATC Cuts at: 2401 2401 Size: 9478 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4351 6115 6148 4351 Size: 1764 33 7681 Fragments arranged by size: 7681 1764 33 FspI TGC'GCA Cuts at: 7984 7984 Size: 9478 HaeII r_GCGC'y Cuts at: 1678 8625 1678 Size: 6947 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2245 2277 3083 3115 3359 Size: 32 135 32 673 32 806 32 244 Cuts at: 3359 3391 3773 3805 4010 4042 4164 4196 4281 Size: 32 382 32 205 32 122 32 85 Cuts at: 4281 4313 4661 4693 5225 5257 5456 5488 6136 Size: 32 348 32 532 32 199 32 648 Cuts at: 6136 6143 6168 6175 6685 6717 8119 8151 8193 Size: 7 25 7 510 32 1402 32 42 Cuts at: 8193 8225 1373 Size: 32 2626 Fragments arranged by size: 2626 1402 806 673 648 532 510 382 348 244 205 199 135 122 85 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 6822 7068 669 Size: 988 281 4884 246 3079 Fragments arranged by size: 4884 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9478 HpaI GTT'AAC Cuts at: 7068 7068 Size: 9478 KpnI G_GTAC'C Cuts at: 6803 6803 Size: 9478 MfeI C'AATT_G Cuts at: 7077 7077 Size: 9478 MluI A'CGCG_T Cuts at: 9354 9354 Size: 9478 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2247 4195 4578 8592 8776 1305 Size: 942 1948 383 4014 184 2007 Fragments arranged by size: 4014 2007 1948 942 383 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2922 3653 4074 4481 10 Size: 54 1425 343 1090 731 421 407 5007 Fragments arranged by size: 5007 1425 1090 731 421 407 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2072 2225 3647 3653 3818 3992 5522 Size: 648 906 153 1422 6 165 174 1530 Cuts at: 5522 6417 6666 7436 7795 7954 518 Size: 895 249 770 359 159 2042 Fragments arranged by size: 2042 1530 1422 906 895 770 648 359 249 174 165 159 153 6 MspA1I CmG'CkG Cuts at: 1606 2680 3163 3592 4849 5554 5777 5965 6368 Size: 1074 483 429 1257 705 223 188 403 Cuts at: 6368 6627 6894 7571 8719 8964 1606 Size: 259 267 677 1148 245 2120 Fragments arranged by size: 2120 1257 1148 1074 705 677 483 429 403 267 259 245 223 188 NcoI C'CATG_G Cuts at: 513 1065 2223 5574 513 Size: 552 1158 3351 4417 Fragments arranged by size: 4417 3351 1158 552 NdeI CA'TA_TG Cuts at: 387 2071 4798 6663 387 Size: 1684 2727 1865 3202 Fragments arranged by size: 3202 2727 1865 1684 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9478 NotI GC'GGCC_GC Cuts at: 6925 6925 Size: 9478 NsiI A_TGCA'T Cuts at: 3100 5059 5755 3100 Size: 1959 696 6823 Fragments arranged by size: 6823 1959 696 NspI r_CATG'y Cuts at: 1132 1229 2991 3253 3523 4239 4425 4444 5312 Size: 97 1762 262 270 716 186 19 868 Cuts at: 5312 6836 8381 1132 Size: 1524 1545 2229 Fragments arranged by size: 2229 1762 1545 1524 868 716 270 262 186 97 19 PasI CC'CwG_GG Cuts at: 1348 3832 1348 Size: 2484 6994 PciI A'CATG_T Cuts at: 4235 4440 8377 4235 Size: 205 3937 5336 Fragments arranged by size: 5336 3937 205 PflMI CCAn_nnn'nTGG Cuts at: 1108 2424 3182 1108 Size: 1316 758 7404 Fragments arranged by size: 7404 1316 758 PfoI T'CCnGG_A Cuts at: 3210 3711 5820 6135 3210 Size: 501 2109 315 6553 Fragments arranged by size: 6553 2109 501 315 PmeI GTTT'AAAC Cuts at: 6782 6782 Size: 9478 PmlI CAC'GTG Cuts at: 3382 4225 3382 Size: 843 8635 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3938 3970 7547 7579 9060 9092 3938 Size: 32 3577 32 1481 32 4324 Fragments arranged by size: 4324 3577 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3068 3068 Size: 9478 PshAI GACnn'nnGTC Cuts at: 1613 2178 1613 Size: 565 8913 PsiI TTA'TAA Cuts at: 7048 7048 Size: 9478 PspOMI G'GGCC_C Cuts at: 4880 5262 6465 6515 4880 Size: 382 1203 50 7843 Fragments arranged by size: 7843 1203 382 50 PspXI vC'TCGA_Gb Cuts at: 4308 4308 Size: 9478 PstI C_TGCA'G Cuts at: 830 3168 3402 3530 3591 3601 5608 5628 6159 Size: 2338 234 128 61 10 2007 20 531 Cuts at: 6159 6830 830 Size: 671 3478 Fragments arranged by size: 3478 2338 2007 671 531 234 128 61 20 10 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9478 PvuII CAG'CTG Cuts at: 2680 3163 4849 5554 5965 6627 2680 Size: 483 1686 705 411 662 5531 Fragments arranged by size: 5531 1686 705 662 483 411 SacI G_AGCT'C Cuts at: 5658 5892 5658 Size: 234 9244 SalI G'TCGA_C Cuts at: 1936 6820 1936 Size: 4884 4594 SapI GCTCTTCn'nnn_ Cuts at: 2093 3360 6137 2093 Size: 1267 2777 5434 Fragments arranged by size: 5434 2777 1267 SbfI CC_TGCA'GG Cuts at: 3402 6830 3402 Size: 3428 6050 ScaI AGT'ACT Cuts at: 7726 9438 7726 Size: 1712 7766 SfcI C'TryA_G Cuts at: 826 1045 2109 3164 3398 3526 3587 3597 5604 Size: 219 1064 1055 234 128 61 10 2007 Cuts at: 5604 5624 6155 6826 7961 8642 8833 826 Size: 20 531 671 1135 681 191 1471 Fragments arranged by size: 2007 1471 1135 1064 1055 681 671 531 234 219 191 128 61 20 10 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9478 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9478 SmaI CCC'GGG Cuts at: 1872 2702 6566 6805 1872 Size: 830 3864 239 4545 Fragments arranged by size: 4545 3864 830 239 SmlI C'TyrA_G Cuts at: 820 1017 1943 3356 4308 6008 6353 7582 8483 Size: 197 926 1413 952 1700 345 1229 901 Cuts at: 8483 8745 820 Size: 262 1553 Fragments arranged by size: 1700 1553 1413 1229 952 926 901 345 262 197 SnaBI TAC'GTA Cuts at: 493 7269 493 Size: 6776 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9478 SphI G_CATG'C Cuts at: 1132 3253 3523 5312 6836 1132 Size: 2121 270 1789 1524 3774 Fragments arranged by size: 3774 2121 1789 1524 270 SspI AAT'ATT Cuts at: 5 52 7402 5 Size: 47 7350 2081 Fragments arranged by size: 7350 2081 47 StuI AGG'CCT Cuts at: 1517 3404 3492 4781 6678 1517 Size: 1887 88 1289 1897 4317 Fragments arranged by size: 4317 1897 1887 1289 88 StyI C'CwwG_G Cuts at: 513 1065 2223 2454 2952 2997 3064 3147 3846 Size: 552 1158 231 498 45 67 83 699 Cuts at: 3846 4069 5574 5982 6238 6618 6645 6917 513 Size: 223 1505 408 256 380 27 272 3074 Fragments arranged by size: 3074 1505 1158 699 552 498 408 380 272 256 231 223 83 67 45 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2577 5975 6251 7505 7522 7675 7860 Size: 1255 997 3398 276 1254 17 153 185 Cuts at: 7860 8305 325 Size: 445 1498 Fragments arranged by size: 3398 1498 1255 1254 997 445 276 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2086 3775 4332 Size: 274 80 33 51 1443 109 1689 557 Cuts at: 4332 4437 5878 6183 6474 7724 9436 96 Size: 105 1441 305 291 1250 1712 138 Fragments arranged by size: 1712 1689 1443 1441 1250 557 305 291 274 138 109 105 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2894 2926 3117 3149 4087 4119 9438 9470 2894 Size: 32 191 32 938 32 5319 32 2902 Fragments arranged by size: 5319 2902 938 191 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9478 XbaI T'CTAG_A Cuts at: 6814 6814 Size: 9478 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2930 2930 Size: 9478 XhoI C'TCGA_G Cuts at: 1943 4308 1943 Size: 2365 7113 XmaI C'CCGG_G Cuts at: 1870 2700 6564 6803 1870 Size: 830 3864 239 4545 Fragments arranged by size: 4545 3864 830 239 XmnI GAAnn'nnTTC Cuts at: 3328 4253 4637 4662 6131 7607 3328 Size: 925 384 25 1469 1476 5199 Fragments arranged by size: 5199 1476 1469 925 384 25 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3718 4117 7286 276 Size: 53 83 186 936 2184 399 3169 2468 Fragments arranged by size: 3169 2468 2184 936 399 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbeI BsiWI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI