Restriction map for
pFN21AE0098
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BalI BbrPI
BbsI BciVI BclI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsiEI BsiWI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HpaI
KpnI KspI MaeII MamI MfeI
MluI MluNI MscI MseI MslI
MunI MvnI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tth111I XbaI
XcmI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccIII 438 TCCGGA T^CCGGA
AlwNI 25 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 646 GGGCCC GGGCC^C
AviII 1068 TGCGCA TGC^GCA
BamHI 394 GGATCC G^GATCC
BbeI 853 GGCGCC GGCGC^C
BbvCI 205 GCTGAGG CCTCAGC(-5/-2)
BcgI 961 GCAGCAGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 848 CTAG C^TAG
BglII 731 AGATCT A^GATCT
BsaWI 438 TCCGGA [AT]^CCGG[AT]
BseAI 438 TCCGGA T^CCGGA
BsgI 97 GTGCAG GTGCAG(16/14)
BsmBI 797 GAGACG CGTCTC(1/5)
BsmI 434 GCATTC GAATGC(1/-1)
BspEI 438 TCCGGA T^CCGGA
BsrDI 737 GCAATG GCAATG(2/0)
BsrFI 785 GCCGGC [GA]^CCGG[CT]
BstAPI 24 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 785 GCCGGC [GA]^CCGG[CT]
EaeI 806 TGGCCG [CT]^GGCC[GA]
FspI 1068 TGCGCA TGC^GCA
HaeII 853 GGCGCC [GA]GCGC^[CT]
HgaI 287 GCGTC GACGC(5/10)
HincII 748 GTCAAC GT[CT]^[GA]AC
HindII 748 GTCAAC GT[CT]^[GA]AC
HindIII 656 AAGCTT A^AGCTT
KasI 853 GGCGCC G^GCGCC
MaeI 848 CTAG C^TAG
MroI 438 TCCGGA T^CCGGA
NaeI 785 GCCGGC GCC^GGC
NarI 853 GGCGCC GG^CGCC
NgoMIV 785 GCCGGC G^CCGGC
PaeR7I 43 CTCGAG C^TCGAG
PflMI 328 CCAACAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 129 GGGACCC [GA]G^G[AT]CC[CT]
PstI 944 CTGCAG CTGCA^G
SfoI 853 GGCGCC GGC^GCC
SmaI 684 CCCGGG CCC^GGG
TaqI 44 TCGA T^CGA
Tsp45I 836 GTCAC ^GT[GC]AC
TthHB8I 44 TCGA T^CGA
Van91I 328 CCAACAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 43 CTCGAG C^TCGAG
XmaCI 684 CCCGGG C^CCGGG
XmaI 684 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 286 GGCGTC G[GA]^CG[CT]C
853 GGCGCC
AfaI 605 GTAC GT^AC
1095 GTAC
AspHI 109 GTGCTC G[AT]GC[AT]^C
548 GTGCTC
BanI 791 GGCACC G^G[CT][GA]CC
853 GGCGCC
BanII 640 GGGCTC G[GA]GC[CT]^C
646 GGGCCC
BsaHI 286 GGCGTC G[GA]^CG[CT]C
853 GGCGCC
BseRI 517 GAGGAG GAGGAG(10/8)
520 GAGGAG
BsiHKAI 109 GTGCTC G[AT]GC[AT]^C
548 GTGCTC
BsmAI 442 GAGAC GTCTC(1/5)
797 GAGAC
DraII 129 GGGACCC [GA]G^G[ACGT]CC[CT]
672 GGGGCCT
DsaI 86 CCATGG C^C[GA][CT]GG
365 CCATGG
EcoO109I 129 GGGACCC [GA]G^G[ACGT]CC[CT]
672 GGGGCCT
FauI 868 GCGGG CCCGC(4/6)
910 GCGGG
Hin1I 286 GGCGTC G[GA]^CG[CT]C
853 GGCGCC
HinfI 242 GAGTC G^A[ACGT]TC
477 GACTC
MaeIII 457 GTAAC ^GT[ACGT]AC
836 GTCAC
MspA1I 956 CAGCGG C[AC]G^C[GT]G
973 CCGCTG
NcoI 86 CCATGG C^CATGG
365 CCATGG
NlaIII 87 CATG CATG^
366 CATG
PleI 477 GACTC GAGTC(4/5)
242 GAGTC
RsaI 605 GTAC GT^AC
1095 GTAC
SfcI 78 CTACAG C^T[GA][CT]AG
944 CTGCAG
Tsp509I 190 AATT ^AATT
621 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AlwI AvaI AvaII
BbvI BcnI BmyI BpmI BsaJI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI Ksp632I MboI
MboII MflI MnlI MspI MvaI
MwoI NciI NdeII NlaIV Sau3AI
Sau96I ScrFI SmlI StyI TseI
TspRI XhoII