(Circular) (Six-base) MAPSORT of: pFN21AE0098.seq Check: 5480 from: 1 to: 5803 pFN21AE0098 5803 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3613 278 Size: 53 83 186 936 2077 2468 Fragments arranged by size: 2468 2077 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3146 1937 Size: 1209 4594 Acc65I G'GTAC_C Cuts at: 3124 3124 Size: 5803 AclI AA'CG_TT Cuts at: 1563 3930 4303 1563 Size: 2367 373 3063 Fragments arranged by size: 3063 2367 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2059 3866 5250 5518 1632 Size: 427 1807 1384 268 1917 Fragments arranged by size: 1917 1807 1384 427 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5606 AflIII A'CryG_T Cuts at: 1203 4702 5679 1203 Size: 3499 977 1327 Fragments arranged by size: 3499 1327 977 AgeI A'CCGG_T Cuts at: 4634 4634 Size: 5803 AhdI GACnn_n'nnGTC Cuts at: 4532 4532 Size: 5803 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5803 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5803 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2567 2599 2567 Size: 32 5771 AlwNI CAG_nnn'CTG Cuts at: 2035 3546 5118 2035 Size: 1511 1572 2720 Fragments arranged by size: 2720 1572 1511 ApaI G_GGCC'C Cuts at: 2655 2655 Size: 5803 ApaLI G'TGCA_C Cuts at: 3860 5016 3860 Size: 1156 4647 ApoI r'AATT_y Cuts at: 1493 3112 3338 3490 5599 1493 Size: 1619 226 152 2109 1697 Fragments arranged by size: 2109 1697 1619 226 152 AseI AT'TA_AT Cuts at: 160 1032 4357 160 Size: 872 3325 1606 Fragments arranged by size: 3325 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5803 AvaI C'yCGr_G Cuts at: 1870 1943 2048 2648 2689 3128 1870 Size: 73 105 600 41 439 4545 Fragments arranged by size: 4545 600 439 105 73 41 BamHI G'GATC_C Cuts at: 2399 3133 3500 2399 Size: 734 367 4702 Fragments arranged by size: 4702 734 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2796 2858 3124 4579 618 Size: 325 217 627 1009 62 266 1455 1842 Fragments arranged by size: 1842 1455 1009 627 325 266 217 62 BanII G_rGCy'C Cuts at: 1391 2649 2655 3122 1391 Size: 1258 6 467 4072 Fragments arranged by size: 4072 1258 467 6 BbeI G_GCGC'C Cuts at: 2862 2862 Size: 5803 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4832 BbvCI CC'TCA_GC Cuts at: 2211 2211 Size: 5803 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2955 2989 3994 4028 1903 Size: 34 50 34 934 34 1005 34 3678 Fragments arranged by size: 3678 1005 934 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3695 4905 3695 Size: 1210 4593 BclI T'GATC_A Cuts at: 1548 1548 Size: 5803 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4414 136 Size: 107 122 71 631 605 2742 1525 Fragments arranged by size: 2742 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2736 5798 2736 Size: 3062 2741 BlpI GC'TnA_GC Cuts at: 3220 3220 Size: 5803 Bme1580I G_kGCm'C Cuts at: 1790 2655 2879 3864 5020 1790 Size: 865 224 985 1156 2573 Fragments arranged by size: 2573 1156 985 865 224 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5803 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2393 3534 4492 Size: 416 53 83 399 24 976 1141 958 Cuts at: 4492 442 Size: 1753 Fragments arranged by size: 1753 1141 976 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5803 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2325 2469 2651 2770 2982 2999 4463 1239 Size: 1086 144 182 119 212 17 1464 2579 Fragments arranged by size: 2579 1464 1086 212 182 144 119 17 Bpu10I CC'TnA_GC Cuts at: 2211 2211 Size: 5803 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2009 2129 2612 3928 4793 5091 2009 Size: 120 483 1316 865 298 2721 Fragments arranged by size: 2721 1316 865 483 298 120 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4466 882 Size: 617 2967 2219 Fragments arranged by size: 2967 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3594 493 Size: 711 2390 2702 Fragments arranged by size: 2702 2390 711 BsaBI GATnn'nnATC Cuts at: 5797 5797 Size: 5803 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2292 2859 3610 3992 Size: 53 83 186 936 759 567 751 382 Cuts at: 3992 4627 275 Size: 635 1451 Fragments arranged by size: 1451 936 759 751 635 567 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2443 4236 4634 4908 5055 5466 2443 Size: 1793 398 274 147 411 2780 Fragments arranged by size: 2780 1793 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2997 3027 1178 Size: 30 165 30 1594 30 3954 Fragments arranged by size: 3954 1594 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2537 2540 1186 Size: 1351 3 4449 Fragments arranged by size: 4449 1351 3 BseYI C'CCAG_C Cuts at: 2435 2586 2674 2930 5006 5502 2435 Size: 151 88 256 2076 496 2736 Fragments arranged by size: 2736 2076 496 256 151 88 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2123 1163 Size: 573 387 4843 Fragments arranged by size: 4843 573 387 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3253 4014 5042 1540 Size: 275 186 1252 761 1028 2301 Fragments arranged by size: 2301 1252 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2118 2557 3864 3949 5020 2118 Size: 439 1307 85 1071 2901 Fragments arranged by size: 2901 1307 1071 439 85 BsiWI C'GTAC_G Cuts at: 3099 3099 Size: 5803 BsmI GAATG_Cn' Cuts at: 2439 3314 3407 2439 Size: 875 93 4835 Fragments arranged by size: 4835 875 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2796 2796 Size: 5803 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2557 2649 2655 2879 3122 3864 Size: 399 328 439 92 6 224 243 742 Cuts at: 3864 3949 5020 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 439 399 328 243 224 92 85 6 BspEI T'CCGG_A Cuts at: 2443 5466 2443 Size: 3023 2780 BspHI T'CATG_A Cuts at: 3692 5422 3692 Size: 1730 4073 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3158 844 Size: 2314 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3690 1394 Size: 429 1867 3507 Fragments arranged by size: 3507 1867 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2749 4298 4472 65 Size: 1183 1501 1549 174 1396 Fragments arranged by size: 1549 1501 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2790 4447 4634 1700 Size: 110 980 1657 187 2869 Fragments arranged by size: 2869 1657 980 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5803 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5803 BssSI C'ACGA_G Cuts at: 3863 4875 3863 Size: 1012 4791 BstAPI GCAn_nnn'nTGC Cuts at: 794 2035 794 Size: 1241 4562 BstBI TT'CG_AA Cuts at: 4607 4607 Size: 5803 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5803 BstXI CCAn_nnnn'nTGG Cuts at: 2129 2911 2938 2129 Size: 782 27 4994 Fragments arranged by size: 4994 782 27 BstYI r'GATC_y Cuts at: 1972 2031 2308 2399 2736 3133 3500 3885 3902 Size: 59 277 91 337 397 367 385 17 Cuts at: 3902 5354 5440 5452 5798 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 397 385 367 346 337 277 91 86 59 17 12 BtgI C'CryG_G Cuts at: 513 1065 2091 2370 513 Size: 552 1026 279 3946 Fragments arranged by size: 3946 1026 552 279 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5064 Fragments arranged by size: 5064 683 56 BtsI GCAGTG_nn' Cuts at: 2958 3319 4113 4133 2958 Size: 361 794 20 4628 Fragments arranged by size: 4628 794 361 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5803 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5768 DraI TTT'AAA Cuts at: 3107 3107 Size: 5803 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4810 809 Size: 507 3494 1802 Fragments arranged by size: 3494 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2811 3250 4139 Size: 54 1425 50 275 18 981 439 889 Cuts at: 4139 5768 8 Size: 1629 43 Fragments arranged by size: 1629 1425 981 889 439 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3250 1537 Size: 275 1438 4090 Fragments arranged by size: 4090 1438 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2078 2514 3015 3095 3733 1353 Size: 725 436 501 80 638 3423 Fragments arranged by size: 3423 725 638 501 436 80 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4390 4764 4910 387 Size: 1064 138 2801 374 146 1280 Fragments arranged by size: 2801 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2059 2325 2469 2651 2770 2982 2999 Size: 393 427 266 144 182 119 212 17 Cuts at: 2999 3866 4463 5250 5518 1239 Size: 867 597 787 268 1524 Fragments arranged by size: 1524 867 787 597 427 393 268 266 212 182 144 119 17 EcoO109I rG'GnC_Cy Cuts at: 2135 2678 2135 Size: 543 5260 EcoRI G'AATT_C Cuts at: 3112 3490 3112 Size: 378 5425 FspI TGC'GCA Cuts at: 3075 4309 3075 Size: 1234 4569 HaeII r_GCGC'y Cuts at: 1678 2862 4950 1678 Size: 1184 2088 2531 Fragments arranged by size: 2531 2088 1184 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2127 2159 3012 3044 3072 Size: 32 135 32 555 32 853 32 28 Cuts at: 3072 3104 4444 4476 4518 4550 1373 Size: 32 1340 32 42 32 2626 Fragments arranged by size: 2626 1340 853 555 135 42 32 32 32 32 32 32 32 28 HincII GTy'rAC Cuts at: 669 1657 1938 2755 3147 3393 669 Size: 988 281 817 392 246 3079 Fragments arranged by size: 3079 988 817 392 281 246 HindIII A'AGCT_T Cuts at: 748 2661 748 Size: 1913 3890 HpaI GTT'AAC Cuts at: 3393 3393 Size: 5803 KasI G'GCGC_C Cuts at: 2858 2858 Size: 5803 KpnI G_GTAC'C Cuts at: 3128 3128 Size: 5803 MfeI C'AATT_G Cuts at: 3402 3402 Size: 5803 MluI A'CGCG_T Cuts at: 5679 5679 Size: 5803 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2791 4917 5101 1305 Size: 1486 2126 184 2007 Fragments arranged by size: 2126 2007 1486 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3981 Fragments arranged by size: 3981 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3761 4120 4279 518 Size: 648 2595 359 159 2042 Fragments arranged by size: 2595 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2963 2980 3219 3896 5044 5289 1606 Size: 1357 17 239 677 1148 245 2120 Fragments arranged by size: 2120 1357 1148 677 245 239 17 NaeI GCC'GGC Cuts at: 2792 2792 Size: 5803 NarI GG'CG_CC Cuts at: 2859 2859 Size: 5803 NcoI C'CATG_G Cuts at: 513 1065 2091 2370 513 Size: 552 1026 279 3946 Fragments arranged by size: 3946 1026 552 279 NdeI CA'TA_TG Cuts at: 387 387 Size: 5803 NgoMIV G'CCGG_C Cuts at: 2790 2790 Size: 5803 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5803 NotI GC'GGCC_GC Cuts at: 3250 3250 Size: 5803 NspI r_CATG'y Cuts at: 1132 1229 3161 4706 1132 Size: 97 1932 1545 2229 Fragments arranged by size: 2229 1932 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5803 PciI A'CATG_T Cuts at: 4702 4702 Size: 5803 PflMI CCAn_nnn'nTGG Cuts at: 1108 2339 1108 Size: 1231 4572 PmeI GTTT'AAAC Cuts at: 3107 3107 Size: 5803 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3872 3904 5385 5417 3872 Size: 32 1481 32 4258 Fragments arranged by size: 4258 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2135 2135 Size: 5803 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5803 PsiI TTA'TAA Cuts at: 3373 3373 Size: 5803 PspOMI G'GGCC_C Cuts at: 2651 2651 Size: 5803 PspXI vC'TCGA_Gb Cuts at: 2048 2048 Size: 5803 PstI C_TGCA'G Cuts at: 830 2953 3155 830 Size: 2123 202 3478 Fragments arranged by size: 3478 2123 202 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5803 SalI G'TCGA_C Cuts at: 1936 3145 1936 Size: 1209 4594 SanDI GG'GwC_CC Cuts at: 2135 2135 Size: 5803 SbfI CC_TGCA'GG Cuts at: 3155 3155 Size: 5803 ScaI AGT'ACT Cuts at: 4051 5763 4051 Size: 1712 4091 SfcI C'TryA_G Cuts at: 826 1045 2083 2949 3151 4286 4967 5158 826 Size: 219 1038 866 202 1135 681 191 1471 Fragments arranged by size: 1471 1135 1038 866 681 219 202 191 SfoI GGC'GCC Cuts at: 2860 2860 Size: 5803 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5803 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5803 SmaI CCC'GGG Cuts at: 1872 2691 3130 1872 Size: 819 439 4545 Fragments arranged by size: 4545 819 439 SmlI C'TyrA_G Cuts at: 820 1017 1943 2024 2048 2144 2591 3907 4808 Size: 197 926 81 24 96 447 1316 901 Cuts at: 4808 5070 820 Size: 262 1553 Fragments arranged by size: 1553 1316 926 901 447 262 197 96 81 24 SnaBI TAC'GTA Cuts at: 493 3594 493 Size: 3101 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5803 SphI G_CATG'C Cuts at: 1132 3161 1132 Size: 2029 3774 SspI AAT'ATT Cuts at: 5 52 3727 5 Size: 47 3675 2081 Fragments arranged by size: 3675 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5803 StyI C'CwwG_G Cuts at: 513 1065 2065 2091 2370 2466 2892 3242 513 Size: 552 1000 26 279 96 426 350 3074 Fragments arranged by size: 3074 1000 552 426 350 279 96 26 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2733 3830 3847 4000 4185 4630 325 Size: 1255 1153 1097 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1153 1097 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4049 5761 96 Size: 274 80 33 51 1443 2072 1712 138 Fragments arranged by size: 2072 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2997 3029 5763 5795 2997 Size: 32 2734 32 3005 Fragments arranged by size: 3005 2734 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5803 XbaI T'CTAG_A Cuts at: 3139 3139 Size: 5803 XhoI C'TCGA_G Cuts at: 1943 2048 1943 Size: 105 5698 XmaI C'CCGG_G Cuts at: 1870 2689 3128 1870 Size: 819 439 4545 Fragments arranged by size: 4545 819 439 XmnI GAAnn'nnTTC Cuts at: 3932 3932 Size: 5803 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3611 276 Size: 53 83 186 936 2077 2468 Fragments arranged by size: 2468 2077 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BfrBI BplI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SrfI SwaI XcmI