Restriction map for
pFN21AE0098
(Linear) (Six-base) MAPSORT of: KIEE0098.seq Check: 4143 from: 1 to: 1098
KIEE0098.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 1098
Size: 54 1044
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 562 594 1098
Size: 562 32 504
Fragments arranged by size:
562 504 32
AlwNI CAG_nnn'CTG
Cuts at: 0 30 1098
Size: 30 1068
ApaI G_GGCC'C
Cuts at: 0 650 1098
Size: 650 448
AvaI C'yCGr_G
Cuts at: 0 43 643 684 1098
Size: 43 600 41 414
Fragments arranged by size:
600 414 43 41
BamHI G'GATC_C
Cuts at: 0 394 1098
Size: 394 704
BanI G'GyrC_C
Cuts at: 0 791 853 1098
Size: 791 62 245
Fragments arranged by size:
791 245 62
BanII G_rGCy'C
Cuts at: 0 644 650 1098
Size: 644 6 448
Fragments arranged by size:
644 448 6
BbeI G_GCGC'C
Cuts at: 0 857 1098
Size: 857 241
BbvCI CC'TCA_GC
Cuts at: 0 206 1098
Size: 206 892
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 950 984 1098
Size: 950 34 114
Fragments arranged by size:
950 114 34
BglII A'GATC_T
Cuts at: 0 731 1098
Size: 731 367
Bme1580I G_kGCm'C
Cuts at: 0 650 874 1098
Size: 650 224 224
Fragments arranged by size:
650 224 224
BmrI ACTGGGnnnn_n'
Cuts at: 0 388 1098
Size: 388 710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 464 646 765 977 994 1098
Size: 320 144 182 119 212 17 104
Fragments arranged by size:
320 212 182 144 119 104 17
Bpu10I CC'TnA_GC
Cuts at: 0 206 1098
Size: 206 892
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4 124 607 1098
Size: 4 120 483 491
Fragments arranged by size:
491 483 120 4
BsaHI Gr'CG_yC
Cuts at: 0 287 854 1098
Size: 287 567 244
Fragments arranged by size:
567 287 244
BsaWI w'CCGG_w
Cuts at: 0 438 1098
Size: 438 660
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 992 1022 1098
Size: 992 30 76
Fragments arranged by size:
992 76 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 532 535 1098
Size: 532 3 563
Fragments arranged by size:
563 532 3
BseYI C'CCAG_C
Cuts at: 0 430 581 669 925 1098
Size: 430 151 88 256 173
Fragments arranged by size:
430 256 173 151 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 1098
Size: 118 980
BsiHKAI G_wGCw'C
Cuts at: 0 113 552 1098
Size: 113 439 546
Fragments arranged by size:
546 439 113
BsmI GAATG_Cn'
Cuts at: 0 434 1098
Size: 434 664
BsmBI CGTCTCn'nnnn_
Cuts at: 0 791 1098
Size: 791 307
Bsp1286I G_dGCh'C
Cuts at: 0 113 552 644 650 874 1098
Size: 113 439 92 6 224 224
Fragments arranged by size:
439 224 224 113 92 6
BspEI T'CCGG_A
Cuts at: 0 438 1098
Size: 438 660
BsrDI GCAATG_nn'
Cuts at: 0 744 1098
Size: 744 354
BsrFI r'CCGG_y
Cuts at: 0 785 1098
Size: 785 313
BstAPI GCAn_nnn'nTGC
Cuts at: 0 30 1098
Size: 30 1068
BstXI CCAn_nnnn'nTGG
Cuts at: 0 124 906 933 1098
Size: 124 782 27 165
Fragments arranged by size:
782 165 124 27
BstYI r'GATC_y
Cuts at: 0 26 303 394 731 1098
Size: 26 277 91 337 367
Fragments arranged by size:
367 337 277 91 26
BtgI C'CryG_G
Cuts at: 0 86 365 1098
Size: 86 279 733
Fragments arranged by size:
733 279 86
BtsI GCAGTG_nn'
Cuts at: 0 953 1098
Size: 953 145
EaeI y'GGCC_r
Cuts at: 0 806 1098
Size: 806 292
EarI CTCTTCn'nnn_
Cuts at: 0 73 509 1010 1090 1098
Size: 73 436 501 80 8
Fragments arranged by size:
501 436 80 73 8
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 320 464 646 765 977 994 1098
Size: 54 266 144 182 119 212 17 104
Fragments arranged by size:
266 212 182 144 119 104 54 17
EcoO109I rG'GnC_Cy
Cuts at: 0 130 673 1098
Size: 130 543 425
Fragments arranged by size:
543 425 130
FspI TGC'GCA
Cuts at: 0 1070 1098
Size: 1070 28
HaeII r_GCGC'y
Cuts at: 0 857 1098
Size: 857 241
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 122 154 1007 1039 1067 1098
Size: 122 32 853 32 28 31
Fragments arranged by size:
853 122 32 32 31 28
HincII GTy'rAC
Cuts at: 0 750 1098
Size: 750 348
HindIII A'AGCT_T
Cuts at: 0 656 1098
Size: 656 442
KasI G'GCGC_C
Cuts at: 0 853 1098
Size: 853 245
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 786 1098
Size: 786 312
MspA1I CmG'CkG
Cuts at: 0 958 975 1098
Size: 958 17 123
Fragments arranged by size:
958 123 17
NaeI GCC'GGC
Cuts at: 0 787 1098
Size: 787 311
NarI GG'CG_CC
Cuts at: 0 854 1098
Size: 854 244
NcoI C'CATG_G
Cuts at: 0 86 365 1098
Size: 86 279 733
Fragments arranged by size:
733 279 86
NgoMIV G'CCGG_C
Cuts at: 0 785 1098
Size: 785 313
PflMI CCAn_nnn'nTGG
Cuts at: 0 334 1098
Size: 334 764
PpuMI rG'GwC_Cy
Cuts at: 0 130 1098
Size: 130 968
PspOMI G'GGCC_C
Cuts at: 0 646 1098
Size: 646 452
PspXI vC'TCGA_Gb
Cuts at: 0 43 1098
Size: 43 1055
PstI C_TGCA'G
Cuts at: 0 948 1098
Size: 948 150
SanDI GG'GwC_CC
Cuts at: 0 130 1098
Size: 130 968
SfcI C'TryA_G
Cuts at: 0 78 944 1098
Size: 78 866 154
Fragments arranged by size:
866 154 78
SfoI GGC'GCC
Cuts at: 0 855 1098
Size: 855 243
SmaI CCC'GGG
Cuts at: 0 686 1098
Size: 686 412
SmlI C'TyrA_G
Cuts at: 0 19 43 139 586 1098
Size: 19 24 96 447 512
Fragments arranged by size:
512 447 96 24 19
StyI C'CwwG_G
Cuts at: 0 60 86 365 461 887 1098
Size: 60 26 279 96 426 211
Fragments arranged by size:
426 279 211 96 60 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 728 1098
Size: 728 370
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 992 1024 1098
Size: 992 32 74
Fragments arranged by size:
992 74 32
XhoI C'TCGA_G
Cuts at: 0 43 1098
Size: 43 1055
XmaI C'CCGG_G
Cuts at: 0 684 1098
Size: 684 414
Enzymes that do cut:
AcuI AloI AlwNI ApaI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BglII Bme1580I BmrI BpmI Bpu10I
BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BsrDI BsrFI BstAPI BstXI
BstYI BtgI BtsI EaeI EarI Eco57MI EcoO109I FspI
HaeII Hin4I HincII HindIII KasI MmeI MspA1I NaeI
NarI NcoI NgoMIV PflMI PpuMI PspOMI PspXI PstI
SanDI SfcI SfoI SmaI SmlI StyI TaqII TstI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbsI BciVI BclI BfrBI BglI
BlpI BmgBI BmtI BplI BsaI BsaAI BsaBI BsiEI
BsiWI BspHI BspMI BsrBI BsrGI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspAI HpaI KpnI MfeI MluI MscI MslI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PsrI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI
XmnI ZraI